scholarly journals Spatiotemporal Dynamics of Free-Living and Particle-Associated Vibrio Communities in the Northern Chinese Marginal Seas

2019 ◽  
Vol 85 (9) ◽  
Author(s):  
Jinchang Liang ◽  
Jiwen Liu ◽  
Xiaolei Wang ◽  
Heyu Lin ◽  
Jingli Liu ◽  
...  

ABSTRACT Vibrio species are associated with human health and play important roles in the carbon cycle. The interest in the Vibrio ecology in marine pelagic environments has increased in recent years, and the correlations between the Vibrio community structure and various environmental factors have been demonstrated. However, the identification of planktonic Vibrio species and their relationship with particulate matter are unclear. Here, we elucidated the spatiotemporal dynamics of Vibrio diversity and in relation to environmental factors in the northern Chinese marginal seas, which feature complex and ever-changing environmental conditions. Vibrio abundance derived from quantitative PCR analysis was higher in summer (∼1.4 × 106 copies liter−1) than in winter (∼1.9 × 105 copies liter−1). Interestingly, the average amount of free-living (on a 0.22-μm-pore-size filter membrane) Vibrio was higher (∼3.89 times) than that of particle-associated Vibrio (on a 3-μm-pore-size filter membrane), making it likely that the preferential lifestyle of the planktonic Vibrio community was free living. Shifts in Vibrio community composition identified by high-throughput amplicon sequencing of the Vibrio-specific 16S rRNA gene were observed at both spatial and temporal scales, which were mainly driven by temperature, dissolved oxygen, ammonium, salinity, nitrite, and phosphate. The most prominent operational taxonomic units in summer were closely related to Vibrio campbellii and Vibrio caribbeanicus and shifted to those affiliated with Vibrio atlanticus in winter. Our study demonstrated abundant and diverse Vibrio populations in the northern Chinese marginal seas, contributing to a better understanding of their potential ecological roles in these ecosystems. IMPORTANCE The dynamics of Vibrio communities have been shown in many marine habitats that are close to land, including estuary or harbor areas. Here, we investigated the spatiotemporal dynamics of Vibrio populations in the northern Chinese marginal seas, spanning a wide spatial scale. We showed that the abundances of the Vibrio population in the present study were higher than those in most previously studied areas and that Vibrio species are more likely to adopt a free-living lifestyle. Moreover, our results expanded upon previous results by showing a clear shift in the dominant Vibrio species from summer to winter, which was mainly attributable to the reduction in the abundance of dominant species in summer. Overall, this work contributes to the understanding of the ecology of the Vibrio communities in the marginal seas.

2019 ◽  
Vol 85 (10) ◽  
Author(s):  
Xiaolei Wang ◽  
Jiwen Liu ◽  
Bei Li ◽  
Jinchang Liang ◽  
Hao Sun ◽  
...  

ABSTRACTVibriospp. are ubiquitous marine bacteria with high metabolism flexibility and genome plasticity. Previous studies have revealed the dynamics of planktonic vibrios in relation to environmental forces, such as temperature and salinity. However, little is known aboutVibrioecology in benthic environments. Here, we elucidate the abundance, diversity, and spatial distribution ofVibriospp. in sediments of the Chinese marginal seas, with a wide spatial range from north to south covering the Yellow Sea (YS), East China Sea (ECS), and South China Sea (SCS). Quantitative analysis showed thatVibriospp. were most abundant in the SCS (∼9.04 × 105copies/g) compared to the YS (∼1.00 × 105copies/g) and ECS (∼8.86 × 105copies/g).Vibriocommunity compositions derived from Illumina sequencing ofVibrio-specific 16S rRNA genes varied significantly between sampling areas, which was reflected by a strong distance-decay pattern. The spatial distribution ofVibriowas governed by a joint effect of spatial and environmental factors (especially temperature, salinity, and SiO32−), and their respective pure effects explained only a small fraction of the community variation. Moreover, we identified the most prominent operational taxonomic units (OTUs) that were partitioned in these sea areas. WhereasVibrionaceaeOTU20 andPhotobacterium lipolyticumwere prevalent in the YS,Vibrio gigantisandPhotobacterium piscicola, andP. piscicola,Photobacterium lutimaris, andPhotobacterium alginatilyticumwere prevalent in the ECS and SCS, respectively. Our study demonstrated clear spatial heterogeneity ofVibriospp. in sediments of the Chinese marginal seas, laying a foundation for fully understanding the marineVibrioecology and the ecological roles of the species.IMPORTANCEVibriois an important component of natural marine microbial populations in terms of pathogenicity and roles in carbon cycling. Compared to the marine pelagic environment, our knowledge of the diversity and distribution pattern ofVibriospp. in sediment is limited. Here, we show higherVibrioabundance in Chinese marginal seas than in other studied sediments. There was a clear spatial differentiation ofVibrioabundance and community composition in different sea areas. The benthicVibriocommunity displayed a strong distance-decay pattern across a wide spatial range, which was formed under the combined effects of spatial and environmental factors. These results provide deep insights into the ecological dynamics ofVibrioand its environmental controls, facilitating a more comprehensive understanding of the marineVibrioecology.


2020 ◽  
Vol 9 (8) ◽  
Author(s):  
Alecia N. Septer ◽  
Lauren Speare ◽  
Collin K. Coleman ◽  
Stephanie Smith ◽  
Coby Dorsey ◽  
...  

Vibrio species of the Harveyi clade are commonly found in free-living and host-associated marine habitats. Here, we report the draft genome sequence for a Harveyi clade bacterium, Vibrio sp. strain LB10LO1, which was isolated from the Atlantic brief squid Lolliguncula brevis.


2021 ◽  
pp. 104244
Author(s):  
Yongsheng Liu ◽  
Wenjie Xiao ◽  
Jianqiang Wu ◽  
Lulu Han ◽  
Hongrui Zhang ◽  
...  

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Younes Bahammou ◽  
Mounir Kouhila ◽  
Haytem Moussaoui ◽  
Hamza Lamsyehe ◽  
Zakaria Tagnamas ◽  
...  

PurposeThis work aims to study the hydrothermal behavior of mortar cement toward certain environmental factors (ambient air temperature and air velocity) based on its drying kinetics data. The objective is to provide a better understanding and controlling the stability of mortar structures, which integrate the sorption phenomenon, drying process, air pressure and intrinsic characteristics. This leads to predict the comportment of mortar structures in relation with main environmental factors and minimize the risk of cracking mortar structures at an early age.Design/methodology/approachThermokinetic study was carried out in natural and forced convection solar drying at three temperatures 20, 30 and 40°C and three air velocities (1, 3 and 5 m.s-1). The empirical and semiempirical models tested successfully describe the drying kinetics of mortar. These models simulate the drying process of water absorbed by capillarity, which is the most common humidity transfer mechanism in building materials and contain parameters with physical significance, which integrate the effect of several environmental factors and intrinsic characteristics of mortar structures.FindingsThe models simulate the drying process of water absorbed by capillarity, which is the most common humidity transfer mechanism in building materials and contain parameters with physical significance, which integrate the effect of several environmental factors and intrinsic characteristics of mortar structures. The average activation energy obtained expressed the temperature effect on the mortar diffusivity. The drying constant and the diffusion coefficient can be used to predict the influence of these environmental factors on the drying behavior of various building materials and therefore on their durability.Originality/valueEvaluation of the effect of several environmental factors and intrinsic characteristics of mortar structures on their durability.


2021 ◽  
Vol 26 (2) ◽  
pp. 171-194
Author(s):  
Anne-France Pinget

Abstract In Belgium, Dutch as spoken by Francophone learners is relatively frequent in political, commercial or educational contexts. While the characteristics of this second language (L2) variety have been studied extensively, there is to date no systematic report of how it is evaluated by either native speakers of Dutch or non-natives. Previous studies conducted in other language contexts have found that non-natives tend to be very critical towards L2 accents similar to their own. The main goal of the present study is to investigate the extent to which the listener’s first language (L1) impacts ratings of the fluency, accentedness and comprehensibility of L2 Dutch as spoken by Francophone learners and how it impacts the identification of the speakers’ L1. Specifically, we compared ratings by three groups of listeners: Francophone learners of Dutch, native speakers of Belgian Dutch and native speakers of Netherlandic Dutch. Moreover, the extent to which three additional cognitive and environmental factors influence L2 ratings is examined: listeners’ familiarity with the L2 variety, their language aptitude and language proficiency. The results show that the majority of native and non-native listeners recognized the speakers’ L1 (French). Non-native listeners perceived L2 speech as less fluent, less comprehensible and more accented than natives did, which corroborates the previously reported critical attitudes towards a shared L2 accent. Moreover, subtle differences in accent and fluency ratings were found between the Netherlandic Dutch and the Belgian Dutch listeners. No clear effects of other cognitive and environmental factors appeared in the ratings.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Nur Syaedah Kamis ◽  
Norazlina Abd. Wahab

Purpose This paper aims to explore the level of hibah knowledge among Muslims in Kedah and investigate its determinants, consisting of education level, education stream, religiosity, social influence and social media. Design/methodology/approach This study is quantitative in nature. Questionnaires were distributed to collect data from Muslims in Alor Setar, Kedah. In total, 195 questionnaires were collected and data were analyzed using descriptive analysis, correlation analysis and multiple regression analysis. Findings The study finds that Muslims in Alor Setar, Kedah have good knowledge of hibah. Further, education stream, religiosity, social influence and social media were identified as significant factors that influence their knowledge of hibah. Research limitations/implications The first limitation is its narrow focus in surveying Muslims only in Alor Setar, Kedah. The second limitation is the limited number of determinants used in investigating hibah knowledge among Muslims and the techniques used in analyzing the data. Despite these limitations, the study’s findings provide invaluable insights into the factors influencing hibah knowledge among Muslims in Alor Setar, Kedah. Practical implications This study provides insights regarding the significant personal factors and environmental factors to increase Muslims’ knowledge of hibah. The link between the Islamic education stream and hibah knowledge provides a clear indication that Islamic education can curb the economic problems caused by the substantial amounts of frozen and unclaimed assets in Malaysia. A significant relationship between the environmental factors (social influence and social media) and hibah knowledge also implies that the government and private agencies related to Islamic estate planning and management may use these significant determinants as part of the marketing strategy to increase the usage of hibah as an alternative tool for estate planning. Originality/value This study contributes to a better understanding of Muslims’ knowledge about hibah. The government and related agencies in Islamic estate planning and management can now gain better insights into Muslims’ level of knowledge about hibah and the factors influencing their knowledge of hibah as an effective tool for Islamic estate planning and management. Hence, more effective strategies can be recommended to enhance the knowledge of Muslims on hibah. The findings of this study should be of value to the government in its effort to address the increasing number of frozen estates in Malaysia.


2018 ◽  
Vol 7 (14) ◽  
Author(s):  
William G. Miller ◽  
Emma Yee ◽  
James L. Bono

Many Arcobacter spp. are free living and are routinely recovered from marine environments.


2020 ◽  
Vol 25 (50) ◽  
pp. 425-449 ◽  
Author(s):  
Lalita A. Manrai ◽  
Ajay K. Manrai ◽  
Stefanie Friedeborn

Purpose The purpose of this paper is to provide a comprehensive review of the literature and develop a model of the determinants, indicators and effects of destination competitiveness (DC), as well as several propositions. Design/methodology/approach This study thoroughly reviewed extant literature to develop a conceptual model and propositions. Findings Two key findings are listed below. First, 12 different environmental factors are identified and 12 propositions are developed linking these environmental factors to DC. Second, a new indicator of DC is developed, namely, Tourism Attractions-Basics-Context (TABC) model. The TABC model is simple and directly taps into the benefits tourists seek in a destination. Research limitations/implications Directions for future research are discussed in detail in the paper. Practical implications Managerial implications are discussed in detail in the paper. Originality/value The extant research on the topic of DC has been rather fragmented and incomplete in scope. The research presented in this paper addresses these limitations.


2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Shumpei Haginoya ◽  
Aiko Hanayama ◽  
Tamae Koike

Purpose The purpose of this paper was to compare the accuracy of linking crimes using geographical proximity between three distance measures: Euclidean (distance measured by the length of a straight line between two locations), Manhattan (distance obtained by summing north-south distance and east-west distance) and the shortest route distances. Design/methodology/approach A total of 194 cases committed by 97 serial residential burglars in Aomori Prefecture in Japan between 2004 and 2015 were used in the present study. The Mann–Whitney U test was used to compare linked (two offenses committed by the same offender) and unlinked (two offenses committed by different offenders) pairs for each distance measure. Discrimination accuracy between linked and unlinked crime pairs was evaluated using area under the receiver operating characteristic curve (AUC). Findings The Mann–Whitney U test showed that the distances of the linked pairs were significantly shorter than those of the unlinked pairs for all distance measures. Comparison of the AUCs showed that the shortest route distance achieved significantly higher accuracy compared with the Euclidean distance, whereas there was no significant difference between the Euclidean and the Manhattan distance or between the Manhattan and the shortest route distance. These findings give partial support to the idea that distance measures taking the impact of environmental factors into consideration might be able to identify a crime series more accurately than Euclidean distances. Research limitations/implications Although the results suggested a difference between the Euclidean and the shortest route distance, it was small, and all distance measures resulted in outstanding AUC values, probably because of the ceiling effects. Further investigation that makes the same comparison in a narrower area is needed to avoid this potential inflation of discrimination accuracy. Practical implications The shortest route distance might contribute to improving the accuracy of crime linkage based on geographical proximity. However, further investigation is needed to recommend using the shortest route distance in practice. Given that the targeted area in the present study was relatively large, the findings may contribute especially to improve the accuracy of proactive comparative case analysis for estimating the whole picture of the distribution of serial crimes in the region by selecting more effective distance measure. Social implications Implications to improve the accuracy in linking crimes may contribute to assisting crime investigations and the earlier arrest of offenders. Originality/value The results of the present study provide an initial indication of the efficacy of using distance measures taking environmental factors into account.


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