scholarly journals Survival of Lactobacillus reuteri DSM 17938 and Lactobacillus rhamnosus GG in the Human Gastrointestinal Tract with Daily Consumption of a Low-Fat Probiotic Spread

2009 ◽  
Vol 75 (19) ◽  
pp. 6198-6204 ◽  
Author(s):  
Yvonne E. M. Dommels ◽  
Robèr A. Kemperman ◽  
Yvonne E. M. P. Zebregs ◽  
René B. Draaisma ◽  
Arne Jol ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Reuteri ◽  
Lactobacillus Rhamnosus ◽  
Health Benefit ◽  
Human Gastrointestinal Tract ◽  
Target Dose ◽  
Low Fat ◽  
Daily Consumption ◽  
Fecal Samples ◽  
Probiotic Strains

ABSTRACT Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. Therefore, probiotic strains should be able to survive passage through the human gastrointestinal tract. Human gastrointestinal tract survival of probiotics in a low-fat spread matrix has, however, never been tested. The objective of this randomized, double-blind, placebo-controlled human intervention study was to test the human gastrointestinal tract survival of Lactobacillus reuteri DSM 17938 and Lactobacillus rhamnosus GG after daily consumption of a low-fat probiotic spread by using traditional culturing, as well as molecular methods. Forty-two healthy human volunteers were randomly assigned to one of three treatment groups provided with 20 g of placebo spread (n = 13), 20 g of spread with a target dose of 1 × 109 CFU of L. reuteri DSM 17938 (n = 13), or 20 g of spread with a target dose of 5 × 109 CFU of L. rhamnosus GG (n = 16) daily for 3 weeks. Fecal samples were obtained before and after the intervention period. A significant increase, compared to the baseline, in the recovery of viable probiotic lactobacilli in fecal samples was demonstrated after 3 weeks of daily consumption of the spread containing either L. reuteri DSM 17938 or L. rhamnosus GG by selective enumeration. In the placebo group, no increase was detected. The results of selective enumeration were supported by quantitative PCR, detecting a significant increase in DNA resulting from the probiotics after intervention. Overall, our results indicate for the first time that low-fat spread is a suitable carrier for these probiotic strains.

scholarly journals Effect of Cell Concentration on the Persistence in the Human Intestine of Four Probiotic Strains Administered through a Multispecies Formulation

Nutrients ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 285 ◽  
Author(s):  
Valentina Taverniti ◽  
Ranjan Koirala ◽  
Alessandro Dalla Via ◽  
Giorgio Gargari ◽  
Elena Leonardis ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Intervention Study ◽  
Cell Concentration ◽  
Bacterial Cells ◽  
Human Intestine ◽  
Human Gastrointestinal Tract ◽  
Fecal Samples ◽  
Probiotic Strains ◽  
Higher Doses ◽  
Different Doses

Studies devoted to evaluating the outcome of different doses of probiotics are very limited, especially for multistrain formulations. In this context, we performed an intervention study that aimed to compare the effect of the administration of two doses (7 billion and 70 billion bacterial cells) of a multistrain probiotic formulation on the persistence of the four probiotic strains that were present in the product in the fecal samples collected from healthy subjects. The overall persistence of the probiotic strains was significantly higher for the 70 billion formulation than for the 7 billion formulation. Furthermore, probiotic strains were detected earlier and for longer for the 70 billion formulation compared to those for the 7 billion formulation. All probiotic strains were recovered alive from the 70 billion preparation, whereas recovery was not possible in a few fecal samples upon administration of the 7 billion preparation. In addition, the overall number of viable probiotic cells recovered on day 14 (i.e., the last day of consumption) was significantly higher for the 70 billion formulation than that for the 7 billion formulation. Finally, we found that the viability of the probiotic cells was stable over the course of the trial independent of volunteers’ handling, demonstrating good manufacturing of the product. In conclusion, this study demonstrated that strains belonging to different taxa may coexist in the human gastrointestinal tract upon ingestion of a multispecies probiotic formulation. Moreover, this study suggests that higher doses of bacterial cells in probiotic formulations may permit a higher, earlier, and longer recovery of the probiotics in the feces of healthy adults.

scholarly journals The influence of probiotics on the cervical malignancy diagnostics quality

2011 ◽  
Vol 68 (11) ◽  
pp. 956-960 ◽  
Author(s):  
Zivko Perisic ◽  
Natasa Perisic ◽  
Svetlana Golocorbin-Kon ◽  
Dusan Vesovic ◽  
Ana Mitrovic-Jovanovic ◽  
...  
Keyword(s):  
Lactobacillus Reuteri ◽  
False Negative ◽  
Lactobacillus Rhamnosus ◽  
Study Group ◽  
Vaginal Infection ◽  
Cervical Smear ◽  
Vaginal Microflora ◽  
Negative Findings ◽  
Probiotic Strains ◽  
Probiotic Preparation

Background/Aim. Probiotics help to provide an optimum balance in the intestines. Probiotics species competitive block toxic substances and growth of unwanted bacteria and yeast species while they compete for the space and food. Lactogyn? is the first oral probiotics on Serbian market dedicated to maintaining a normal vaginal flora. Lactogyn? contains two well studied probiotics strains - Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14. Both of them are considered as probiotic agents with therapeutic properties increase the population of beneficial lactobacillus organisms within the vagina. The aim of this study was to exam an influence of Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 on results of cervical smear cytological testing including detection of atypical cells, detection of false positive and false negative findings as well as on vaginal microflora content in patients with vaginal infection signs and symptoms. Methods. Totally 250 women with signs of vaginal infection were selected to participate in the study. The study group comprised 125 patients taking studied probiotic strains along with specific anti-infective therapy. The control group comprised, also, 125 patients taking anti-infective agents, only. Probiotic preparation (Lactogyn ? capsules) was administered orally (one capsule daily) during 4 weeks. Before and six weeks after beginning of the therapy a cervical smear cytological test (the Papanicolaou test), as well as microbiological examination of the vaginal smear were performed. Results. Number of cases of inflammation and atypical squamous cells of undeterminated significance (ASCUS) in the study group were significantly higher before administration of the probiotic preparation. The number of lactobacilli was significantly higher, and the number of pathogenic microorganisms lower in the group treated with this preparation. Conclusion. The application of probiotic strains Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 concomitantly with specific antiinfective agents provides more reliable cytological diagnostics, reduces the number of false positive and false negative findings on cervical malignancy and normalizes vaginal microflora in higher percentage of patients with vaginal infections comapred with therapy including anti-infective agents only.

scholarly journals Topical Probiotics for Women’s Urogenital Health: Selection of an Oil-based Carrier

2021 ◽  
Vol 06 (04) ◽  
pp. 1-1
Author(s):  
Scarlett Puebla-Barragan ◽  
◽  
Britney Lamb ◽  
Serenah Jafelice ◽  
Gregor Reid ◽  
...  
Keyword(s):  
Lactobacillus Reuteri ◽  
Lactobacillus Rhamnosus ◽  
Coconut Oil ◽  
Petroleum Jelly ◽  
Vaginal Microbiome ◽  
Topical Product ◽  
Freeze Dried ◽  
Probiotic Strains ◽  
Selection Of

Vaginal care products are widely used by women to relieve discomfort such as pain, itching and malodour, all of which are commonly caused by conditions resulting from microbiota dysbiosis. Previous studies showed that probiotic strains Lacticaseibacillus (formerly Lactobacillus) rhamnosus GR-1 (LGR-1) and Limosilactobacillus (formerly Lactobacillus) reuteri RC-14 (LRC-14), can aid in restoring homeostasis in the vaginal microbiome when taken orally. A topical product containing these strains could be of value for reducing malodour and improving quality of life. However, the formulation of such a product is a challenge, given that its ingredients must maintain shelf-life viability by excluding moisture. Here, we tested petroleum jelly, mineral oil, coconut oil, and olive oil for how well they maintained the viability of freeze-dried probiotic strains over a six-month timeframe. None of the oils caused excessive loss of bacterial viability, with petroleum jelly and coconut oil showing the most promise. Based on existing knowledge of these oils on the female genitalia, coconut oil and petroleum jelly could be suitable probiotic carriers for clinical testing.

Lactobacillus rhamnosus GG in a mixed pineapple (Ananas comosus L. Merril) and jussara (Euterpe edulis Martius) beverage and its survival in the human gastrointestinal tract

LWT ◽  
2020 ◽  
Vol 134 ◽  
pp. 110028 ◽  
Author(s):  
Bruno de Andrade Pires ◽  
Renata Cristina de Almeida Bianchini Campos ◽  
John Warnens Canuto ◽  
Thainá de Melo Carlos Dias ◽  
Eliane Maurício Furtado Martins ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Rhamnosus ◽  
Ananas Comosus ◽  
Human Gastrointestinal Tract ◽  
Lactobacillus Rhamnosus Gg ◽  
Euterpe Edulis ◽  
Euterpe Edulis Martius

scholarly journals Daily probiotics: benefits and reasonable application

2021 ◽  
pp. 136-143
Author(s):  
Yu. O. Shulpekova ◽  
V. Yu. Rusyaev
Keyword(s):  
Lactobacillus Acidophilus ◽  
Lactobacillus Rhamnosus ◽  
Food Products ◽  
Careful Consideration ◽  
Daily Consumption ◽  
Bifidobacterium Lactis ◽  
Alcoholic Fatty Liver ◽  
High Adhesion ◽  
Intestinal Pathogens ◽  
Probiotic Strains

This article reviewed the mechanisms of action of probiotics and the possible effects of individual strains on the general wellbeing if they are taken daily.The content and activity of bacteria in food products should be regulated by special guidelines. The balanced nutrition allows us to get healthy strains in a natural way. Daily consumption of certain strains as part of functional food products is promising for the prevention of obesity, type 2 diabetes mellitus, non-alcoholic fatty liver disease, functional intestinal disorders, colon cancer, cardiovascular diseases and depression. The issues of safety of novel probiotic strains newly introduced in clinical practice require careful consideration. Synbiotics can comprise probiotic strains of bacteria (Lactobacillus acidophilus La-14, Lactobacillus rhamnosus Lr-32, Bifidobacterium lactis Bl-04) with tolerance to acid, pepsin and bile salts, as well as the prebiotic inulin and vitamins B1, B2, B6 and B12 contributing to survival of beneficial bacteria. L. acidophilus is a common probiotic that occurs in the natural environment and food products, no cases of antibiotic resistance of this species have been established. Strains Lactobacillus acidophilus La-14, Lactobacillus rhamnosus Lr-32, Bifidobacterium lactis Bl-04 have a high adhesion capacity, strong inhibitory effects on intestinal pathogens, including fungi, anti-inflammatory effects, help to eliminate oxalates. As can be seen from the above, the use of probiotics and synbiotics is one of the most promising preventive fields of medicine.

scholarly journals Detoxification of Aflatoxin B1 by Probiotic Yeasts and Bacteria Isolated From Dairy Products of Iran

2020 ◽  
Vol 10 (3) ◽  
pp. 482-487
Author(s):  
Hajar Zolfaghari ◽  
Arezou Khezerlou ◽  
Ali Ehsani ◽  
Ahmad Yari Khosroushahi
Keyword(s):  
Gastrointestinal Tract ◽  
Aflatoxin B1 ◽  
Immunosuppressive Agents ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Bacteria ◽  
Enzyme Linked Immunosorbent Assay ◽  
Probiotic Strains ◽  
High Level ◽  
Gastric Secretions ◽  
Harmful Effects

Purpose : The present study was conducted to assess the ability of probiotic bacteria and yeasts strains to reduce aflatoxin B1 (AFB1) in gastrointestinal simulated conditions. Aflatoxins are potent carcinogenic and immunosuppressive agents. Acute exposure to a high level of aflatoxins leads to aflatoxicosis, which cause rapid death due to liver failure. It is anticipated that consumption of probiotic microorganisms capable of binding aflatoxins can reduce the risk of AFB1 on human health to a certain extent. Methods: For this purpose, the bacteria (1 × 1010 cfu/mL) and yeasts count (2 × 108 cells/mL) and AFB1 concentration (10 ppb) were adjusted. Then, the samples were incubated in the simulated medium, human gastric secretions and small intestine. The concentration of residual AFB1 was determined using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by SPSS 16 software. Results: The native isolated bacteria and yeasts in the simulated gastrointestinal tract condition showed a significant effect on AFB1 reduction (P<0.05). The AFB1 reduction ability of native probiotic microorganisms was strain dependent. The highest binding ability in bacteria belonged to Lactobacillus rhamnosus (31.14%) and at yeasts belonged to Saccharomyces cerevisiae (30.46%). Conclusion: The use of probiotic strains is the appropriate biological method to reduce AFB1 in the human gastrointestinal tract. Probiotic bacteria could help to decrease the harmful effects of AFB1 in humans through enhancing the food safety.

Persistence of Colonization of Human Colonic Mucosa by a Probiotic Strain, Lactobacillus rhamnosusGG, after Oral Consumption

1999 ◽  
Vol 65 (1) ◽  
pp. 351-354 ◽  
Author(s):  
Minna Alander ◽  
Reetta Satokari ◽  
Riitta Korpela ◽  
Maija Saxelin ◽  
Terttu Vilpponen-Salmela ◽  
...  
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Fecal Samples ◽  
Colonic Biopsy ◽  
Oral Consumption ◽  
Lactose Fermentation ◽  
Human Volunteers ◽  
Probiotic Strains ◽  
Fermentation Test

ABSTRACT Lactobacillus rhamnosus GG is one of the most thoroughly studied probiotic strains. Its advantages in the treatment of gastrointestinal disorders are well documented. The aim of the present study was to demonstrate with colonic biopsies the attachment of strain GG to human intestinal mucosae and the persistence of the attachment after discontinuation of GG administration. A whey drink fermented with strain GG was fed to human volunteers for 12 days. Fecal samples were collected before, during, and after consumption. L. rhamnosus GG-like colonies were detected in both fecal and colonic biopsy samples. Strain GG was identified by its characteristic colony morphology, a lactose fermentation test, and PCR. This study showed that strain GG was able to attach in vivo to colonic mucosae and, although the attachment was temporary, to remain for more than a week after discontinuation of GG administration. The results demonstrate that the study of fecal samples alone is not sufficient in evaluating colonization by a probiotic strain.

scholarly journals Cholesterol Assimilation byLactobacillusProbiotic Bacteria: AnIn VitroInvestigation

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Catherine Tomaro-Duchesneau ◽  
Mitchell L. Jones ◽  
Divya Shah ◽  
Poonam Jain ◽  
Shyamali Saha ◽  
...  
Keyword(s):  
Lactobacillus Reuteri ◽  
Culture Media ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Bacteria ◽  
Bile Salt Hydrolase ◽  
Dietary Interventions ◽  
Cholesterol Levels ◽  
Probiotic Strains ◽  
Therapeutic Measures ◽  
Pharmaceutical Agents

Excess cholesterol is associated with cardiovascular diseases (CVD), an important cause of mortality worldwide. Current CVD therapeutic measures, lifestyle and dietary interventions, and pharmaceutical agents for regulating cholesterol levels are inadequate. Probiotic bacteria have demonstrated potential to lower cholesterol levels by different mechanisms, including bile salt hydrolase activity, production of compounds that inhibit enzymes such as 3-hydroxy-3-methylglutaryl coenzyme A, and cholesterol assimilation. This work investigates 11Lactobacillusstrains for cholesterol assimilation. Probiotic strains for investigation were selected from the literature:Lactobacillus reuteriNCIMB 11951,L. reuteriNCIMB 701359,L. reuteriNCIMB 702655,L. reuteriNCIMB 701089,L. reuteriNCIMB 702656,Lactobacillus fermentumNCIMB 5221,L. fermentumNCIMB 8829,L. fermentumNCIMB 2797,Lactobacillus rhamnosusATCC 53103 GG,Lactobacillus acidophilusATCC 314, andLactobacillus plantarumATCC 14917. Cholesterol assimilation was investigated in culture media and under simulated intestinal conditions. The best cholesterol assimilator wasL. plantarumATCC 14917 (15.18 ± 0.55 mg/1010 cfu) in MRS broth.L. reuteriNCIMB 701089 assimilated over 67% (2254.70 ± 63.33 mg/1010 cfu) of cholesterol, the most of all the strains, under intestinal conditions. This work demonstrates that probiotic bacteria can assimilate cholesterol under intestinal conditions, withL. reuteriNCIMB 701089 showing great potential as a CVD therapeutic.

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