scholarly journals Importance of Passive Diffusion in the Uptake of Polychlorinated Biphenyls by Phagotrophic Protozoa

2000 ◽  
Vol 66 (5) ◽  
pp. 1987-1993 ◽  
Author(s):  
Elizabeth B. Kujawinski ◽  
John W. Farrington ◽  
James W. Moffett
Keyword(s):  
Steady State ◽  
Numerical Model ◽  
Organic Carbon ◽  
Polychlorinated Biphenyls ◽  
Size Class ◽  
Relative Importance ◽  
Uptake Rates ◽  
Laboratory Cultures ◽  
Aqueous Solubilities ◽  
Protozoan Cell

ABSTRACT Unicellular protozoan grazers represent a size class of organisms where a transition in the mechanism of chlorobiphenyl (CB) introduction, from diffusion through surface membranes to ingestion of contaminated prey, could occur. This study compares the relative importance of these two processes in the overall uptake of polychlorinated biphenyls by protists. Uptake rates and steady-state concentrations were compared in laboratory cultures of grazing and nongrazing protozoa. These experiments were conducted with a 10-μm marine scuticociliate (Uronema sp.), bacterial prey (Halomonas halodurans), and a suite of 21 CB congeners spanning a range of aqueous solubilities. The dominant pathway of CB uptake by both grazing and nongrazing protozoa was diffusion. Organic-carbon-normalized CB concentrations (in the protozoan cell) were equivalent in grazing and nongrazing protozoa for all congeners studied. Rate constants for uptake into and loss from the protozoan cell were independently determined by using [3,3′,4,4′-14C]tetrachlorobiphenyl (IUPAC no. 77), 0.38 ± 0.03 min−1 and (1.1 ± 0.1) × 10−5 (g of organic carbon)−1min−1, respectively. Magnitudes of the uptake and loss processes were calculated and compared by using a numerical model. The model result was consistent with data from the bioaccumulation experiment and supported the hypothesis that diffusive uptake is faster than ingestive uptake in phagotrophic unicellular protozoa.

scholarly journals Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity

2005 ◽  
Vol 169 (4) ◽  
pp. 569-576 ◽  
Author(s):  
Clare L. Bennett ◽  
Erwin van Rijn ◽  
Steffen Jung ◽  
Kayo Inaba ◽  
Ralph M. Steinman ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Steady State ◽  
Diphtheria Toxin ◽  
Langerhans Cells ◽  
Contact Hypersensitivity ◽  
Relative Importance ◽  
Skin Immunity

Langerhans cells (LC) form a unique subset of dendritic cells (DC) in the epidermis but so far their in vivo functions in skin immunity and tolerance could not be determined, in particular in relation to dermal DC (dDC). Here, we exploit a novel diphtheria toxin (DT) receptor (DTR)/DT-based system to achieve inducible ablation of LC without affecting the skin environment. Within 24 h after intra-peritoneal injection of DT into Langerin-DTR mice LC are completely depleted from the epidermis and only begin to return 4 wk later. LC deletion occurs by apoptosis in the absence of inflammation and, in particular, the dDC compartment is not affected. In LC-depleted mice contact hypersensitivity (CHS) responses are significantly decreased, although ear swelling still occurs indicating that dDC can mediate CHS when necessary. Our results establish Langerin-DTR mice as a unique tool to study LC function in the steady state and to explore their relative importance compared with dDC in orchestrating skin immunity and tolerance.

scholarly journals Particulate organic carbon (POC) in relation to other pore water carbon fractions in drained and rewetted fens in Southern Germany

2008 ◽  
Vol 5 (6) ◽  
pp. 1615-1623 ◽  
Author(s):  
S. Fiedler ◽  
B. S. Höll ◽  
A. Freibauer ◽  
K. Stahr ◽  
M. Drösler ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Particulate Organic Carbon ◽  
Pore Water ◽  
Inorganic Carbon ◽  
Dissolved Inorganic Carbon ◽  
Relative Importance ◽  
Pore Waters ◽  
Calcareous Fens ◽  
Essential Components ◽  
The Individual

Abstract. Numerous studies have dealt with carbon (C) contents in Histosols, but there are no studies quantifying the relative importance of the individual C components in pore waters. For this study, measurements were taken of all the carbon components (particulate organic carbon, POC; dissolved organic carbon, DOC; dissolved inorganic carbon, DIC; dissolved methane, CH4) in the soil pore water of calcareous fens under three different water management regimes (re-wetted, deeply and moderately drained). Pore water was collected weekly or biweekly (April 2004 to April 2006) at depths between 10 and 150 cm. The main results obtained were: (1) DIC (94–280 mg C l−1) was the main C-component. (2) POC and DOC concentrations in the pore water (14–125 mg C l−1 vs. 41–95 mg C l−1) were pari passu. (3) Dissolved CH4 was the smallest C component (0.005–0.9 mg C l−1). Interestingly, about 30% of the POM particles were colonized by microbes indicating that they are active in the internal C turnover. Certainly, both POC and DOC fractions are essential components of the C budget of peatlands. Furthermore, dissolved CO2 in all forms of DIC appears to be an important part of peatland C-balance.

The Numerical Analysis of Temperature Field During Moveable Induction Heating of Steel Plate

2012 ◽  
Vol 28 (02) ◽  
pp. 73-81
Author(s):  
Xue-biao Zhang ◽  
Yu-long Yang ◽  
Yu-jun Liu
Keyword(s):  
Temperature Field ◽  
Steady State ◽  
Numerical Model ◽  
Induction Heating ◽  
Temperature Difference ◽  
Steel Plate ◽  
Heating Process ◽  
Quasi Steady State ◽  
Initial State ◽  
Plate Temperature

In shipyards, hull curved plate formation is an important stage with respect to productivity and accuracy control of curved plates. Because the power and its distribution of induction heat source are easier to control and reproduce, induction heating is expected to be applied in the line heating process. This paper studies the moveable induction heating process of steel plate and develops a numerical model of electromagneticthermal coupling analysis and the numerical results consistent with the experimental results. The numerical model is used to analyze the temperature changing rules and the influences on plate temperature field of heating speed of moveable induction heating of steel plate, and the following conclusions are drawn. First, the process of moveable induction heating of steel plate can be divided into three phases of initial state, quasi-steady state, and end state. The temperature difference between the top and bottom surfaces of the steel plate at the initial state is the biggest; it remains unchanged at the quasi-steady state and it is the smallest at the end state. Second, obvious end effect occurs when the edges of the steel plate are heated by the inductor, which causes a decrease in temperature difference between the top and bottom surfaces of the steel plate that is unfavorable for formation of pillow shape plates. Third, with the increase of heating speed, the temperature difference between the top and bottom surfaces of the steel plate increases gradually.

scholarly journals Fluvial organic carbon flux from an eroding peatland catchment, southern Pennines, UK

2008 ◽  
Vol 12 (2) ◽  
pp. 625-634 ◽  
Author(s):  
R. R. Pawson ◽  
D. R. Lord ◽  
M. G. Evans ◽  
T. E. H. Allott
Keyword(s):  
Organic Carbon ◽  
Carbon Flux ◽  
Relative Importance ◽  
Carbon Export ◽  
Event Scale ◽  
Organic Carbon Flux ◽  
Poc Export ◽  
Event Based ◽  
First Time ◽  
Annual Flux

Abstract. This study investigates for the first time the relative importance of dissolved organic carbon (DOC) and particulate organic carbon (POC) in the fluvial carbon flux from an actively eroding peatland catchment in the southern Pennines, UK. Event scale variability in DOC and POC was examined and the annual flux of fluvial organic carbon was estimated for the catchment. At the event scale, both DOC and POC were found to increase with discharge, with event based POC export accounting for 95% of flux in only 8% of the time. On an annual cycle, exports of 35.14 t organic carbon (OC) are estimated from the catchment, which represents an areal value of 92.47 g C m−2 a−1. POC was the most significant form of organic carbon export, accounting for 80% of the estimated flux. This suggests that more research is required on both the fate of POC and the rates of POC export in eroding peatland catchments.

Use of a Self-Cleaning, In-line Filter to Continuously Monitor Phytoplankton Nutrient Uptake Rates

1984 ◽  
Vol 41 (3) ◽  
pp. 540-544 ◽  
Author(s):  
J. S. Parslow ◽  
P. J. Harrison ◽  
P. A. Thompson
Keyword(s):  
Nutrient Uptake ◽  
Uptake Rate ◽  
Skeletonema Costatum ◽  
Thalassiosira Pseudonana ◽  
Ammonium Concentration ◽  
Marine Diatoms ◽  
Response Characteristics ◽  
Uptake Rates ◽  
Laboratory Cultures ◽  
Self Cleaning

Our new technique allows the decline in ambient ammonium concentration to be followed continuously during phytoplankton uptake experiments. A self-cleaning, in-line filter permits the continuous separation of cells from the medium for nutrient determination by an autoanalyzer. The technique works well with laboratory cultures of the marine diatoms Thalassiosira pseudonana and Skeletonema costatum. Changes in uptake rate on time scales of 1 min or longer can be resolved, the resolution being limited by the response characteristics of the autoanalyzer.

scholarly journals Relative importance of CO2 recycling and CH4 pathways in lake food webs along a dissolved organic carbon gradient

2006 ◽  
Vol 51 (4) ◽  
pp. 1602-1613 ◽  
Author(s):  
Jay T. Lennon ◽  
Anthony M. Faiia ◽  
Xiahong Feng ◽  
Kathryn L. Cottingham
Keyword(s):  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Food Webs ◽  
Relative Importance ◽  
Co2 Recycling

scholarly journals Effect of Rotor Spacing and Duct Diffusion Angle on the Aerodynamic Performances of a Counter-Rotating Ducted Fan in Hover Mode

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1338
Author(s):  
Woo-Yul Kim ◽  
Santhosh Senguttuvan ◽  
Sung-Min Kim
Keyword(s):  
Steady State ◽  
Numerical Model ◽  
Figure Of Merit ◽  
Aerodynamic Performance ◽  
Power Coefficient ◽  
Flow Conditions ◽  
Thrust Coefficient ◽  
Ducted Fan ◽  
Aerodynamic Performances ◽  
Rotor Model

The aerodynamic performance of a counter-rotating ducted fan in hover mode is numerically analyzed for different rotor spacings and duct diffusion angles. The design of the counter-rotating fan is inspired by a custom-designed single rotor ducted fan used in a previous study. The numerical model to predict the aerodynamic performance of the counter-rotating ducted fan is developed by adopting the frozen rotor approach for steady-state incompressible flow conditions. The relative angle between the front and the rear rotor is examined due to the usage of the frozen rotor model. The results show that the variation of thrust for the different relative angles is extremely low. The aerodynamic performances are evaluated by comparing the thrust, thrust coefficient, power coefficient, and figure of merit (FOM). The thrust, thrust coefficient, and FOM slightly increase with increasing rotor spacing up to 200 mm, regardless of the duct diffusion angle, and reduce on further increase in the rotor spacing. The duct diffusion angle of 0° generates about 9% higher thrust and increases the FOM by 6.7%, compared with the 6° duct diffusion angle. The duct diffusion angle is highly effective in improving the thrust and FOM of the counter-rotating ducted fan, rather than the rotor spacing.

Modeling dynamics of organic carbon and nitrogen removal during aeration interruption in aerated horizontal flow treatment wetlands

2019 ◽  
Vol 80 (3) ◽  
pp. 597-606 ◽  
Author(s):  
Johannes Boog ◽  
Thomas Kalbacher ◽  
Jaime Nivala ◽  
Manfred van Afferden ◽  
Roland A. Müller
Keyword(s):  
Steady State ◽  
Organic Carbon ◽  
Dynamic Response ◽  
Nitrogen Removal ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Treatment Wetlands ◽  
Horizontal Flow ◽  
Simulation Accuracy ◽  
Carbon And Nitrogen

Abstract Despite recent developments in process-based modeling of treatment wetlands (TW), the dynamic response of horizontal flow (HF) aerated wetlands to interruptions of aeration has not yet been modeled. In this study, the dynamic response of organic carbon and nitrogen removal to interruptions of aeration in an HF aerated wetland was investigated using a recently-developed numerical process-based model. Model calibration and validation were achieved using previously obtained data from pilot-scale experiments. Setting initial concentrations for anaerobic bacteria to high values ( 35–70 mg L−1) and including ammonia sorption was important to simulate the treatment performance of the experimental wetland in transition phases when aeration was switched off and on again. Even though steady-state air flow rate impacted steady-state soluble chemical oxygen demand (CODs), ammonia nitrogen (NH4–N) and oxidized nitrogen (NOx–N) concentration length profiles, it did not substantially affect corresponding effluent concentrations during aeration interruption. When comparing simulated with experimental results, it is most likely that extending the model to include mass transfer through the biofilm will allow to better explain the underlying experiments and to increase simulation accuracy. This study provides insights into the dynamic behavior of HF aerated wetlands and discusses assumptions and limitations of the modeling approach.

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