scholarly journals Genome Sequences of Klebsiella variicola Isolates from Dairy Animals with Bovine Mastitis from Newfoundland, Canada

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Fraser W. Davidson ◽  
Hugh G. Whitney ◽  
Kapil Tahlan

Klebsiella variicola was recently reported as an emerging and/or previously misidentified species associated with opportunistic infections in humans. Here, we report the draft genome sequences of K. variicola isolates from two animals with clinical mastitis from a dairy farm in Newfoundland, Canada.

2018 ◽  
Vol 7 (5) ◽  
Author(s):  
Paul Cormican ◽  
Orla M. Keane

This is the announcement of draft genome sequences for Staphylococcus aureus strains belonging to sequence type 97 (ST97) and ST71. These sequence types are commonly associated with bovine mastitis, and the strains were isolated in Ireland in 2010 from the milk of cows with clinical mastitis.


2020 ◽  
Vol 9 (19) ◽  
Author(s):  
Soyoun Park ◽  
Dongyun Jung ◽  
Simon Dufour ◽  
Jennifer Ronholm

Staphylococcus aureus is one of the most common etiological agents responsible for contagious bovine mastitis. Here, we report the draft whole-genome sequences, with annotations, of 27 S. aureus strains and 3 Staphylococcus species strains that were isolated from Holstein cows with intramammary infection in Canada.


Author(s):  
R. Waseem ◽  
A. Muhee ◽  
H. U. Malik ◽  
Z. A. Akhoon ◽  
Khusheeba Munir ◽  
...  

Background: Mastitis is one of the most economically important diseases of dairy animals causing heavy losses in terms of fall in milk production, decreased milk quality and additional costs for veterinary services. Mastitis is caused by a wide variety of microorganisms including bacteria, fungi, yeast and mycoplasma, of which bacteria are the most frequently isolated pathogens. Microbiological testing is necessary for identification of cause of mastitis and adaptation of control measures to prevent the disease. The aim of the presnt study was to isolate and identify major mastitis causing bacteria from clinical mastitic cases presented to Veterinary Clinical complex, FVSc. and AH, Shuhama Kashmir for treatment. Methods: A total of 200 bovine milk samples from CMT confirmed clinical mastitis cases were collected and cultured on Nutrient agar, Blood agar, Mac-Conkey agar, Eosine Methylene Blue (EMB) agar, Mannitol salt agar and Edwards media for bacterial isolation. The isolates were then subjected to biochemical tests for identification. Result: Upon microbiological testing and biochemical confirmation, the major mastitis causing bacteria isolated from clinical cases of Bovine mastitis were Staphylococcus Spp. showing highest prevalence of 46.4% followed by mixed infection with Staphylococcus and Streptococcus (20.8%), Streptococci Spp. (18.4%) and Escherichia coli (14.4%).


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Danielle Mendes Silva ◽  
Mônica Pacheco da Silva ◽  
Pedro M. Pereira Vidigal ◽  
Rafael Mazioli Barcelos ◽  
Raphael Contelli Klein ◽  
...  

2021 ◽  
Vol 10 (7) ◽  
Author(s):  
Dongyun Jung ◽  
Soyoun Park ◽  
Janina Ruffini ◽  
Simon Dufour ◽  
Jennifer Ronholm

ABSTRACT Escherichia coli is one of the most common etiological agents responsible for clinical bovine mastitis. Here, we report the draft genome sequences and annotations of 113 E. coli strains that were isolated from Holstein cows with intramammary infections in Canada.


2020 ◽  
Vol 5 (1) ◽  
pp. 1-8
Author(s):  
Adem Kumbe

A cross-sectional study was conducted to determine the prevalence of bovine mastitis, associated potential risk factors and major etiological agents of clinical and sub clinical mastitis under different management condition of pastoral, agro-pastoral, ranch and farm by using California mastitis test and bacteriology. A total of 384 lactating Borana cows in Did-tuyura ranch, Yabello Pastoral and dryland agriculture research center (YPDARC) dairy farm and three districts namely Gomole, Moyale and Yabello of Borana zone were included in the study. The study revealed that overall prevalence of mastitis were 47.4 % (182/384); out of which 12 % (46/384) clinical and 35.4 % (136/384) sub-clinical mastitis whereas prevalence at quarter level was 21.48% (330/1536) of which 3% (46/1536) and 18.48% (284/1536) were clinical and sub-clinical form respectively. From the total examined quarter, 3.5% (53) of quarters had blind quarter. Prevalence in pastoral and agro-pastoral herding system (extensive management system) at cow level and quarter level were 18.9% and 10.9% respectively while prevalence in Did-tuyura ranch and YPDARC dairy farm herding system (semi-intensive) were 20% and 7% at cow level and quarter level respectively. The prevalence of mastitis significantly (P<0.05) differed with parity, stage of lactation and body condition of lactating animals. From 330 California Mastitis Test (CMT) and clinically positive milk samples there was growth of bacteria on culture media observed only in 155 (46.97%). Out of this Staphylococcus aureus accounted for 59 (38.06%) isolates followed by Streptococcus species 33 (21.29%) and Coagulase negative Staphylococcus 30 (19.35%). Due to lack of proper managements of different risk factors major pathogenic microorganisms are isolated. Proper preventive and control strategy, awareness creation on key factors of mastitis, Regular screening and culling of chronically infected cows should be practiced.


Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 644
Author(s):  
Valentina Monistero ◽  
Antonio Barberio ◽  
Paola Cremonesi ◽  
Bianca Castiglioni ◽  
Stefano Morandi ◽  
...  

Streptococcus uberis, an environmental pathogen responsible also for contagious transmission, has been increasingly implicated in clinical mastitis (CM) cases in Europe. We described a 4-month epidemiological investigation of Strep. uberis CM cases in an Italian dairy farm. We determined molecular characteristics and phenotypic antimicrobial resistance of 71 Strep. uberis isolates from dairy cows with CM. Genotypic variability was investigated via multiplex PCR of housekeeping and virulence genes, and by RAPD-PCR typing. Antimicrobial susceptibility was assessed for 14 antimicrobials by MIC assay. All the isolates carried the 11 genes investigated. At 90% similarity, two distinct clusters, grouping 69 of the 71 isolates, were detected in the dendrogram derived from the primer ERIC1. The predominant cluster I could be separated into two subclusters, containing 38 and 14 isolates, respectively. Strep. uberis strains belonging to the same RAPD pattern differed in their resistance profiles. Most (97.2%) of them were resistant to at least one of the drugs tested, but only 25.4% showed a multidrug resistance phenotype. The highest resistance rate was observed for lincomycin (93%), followed by tetracycline (85.9%). This study confirmed a low prevalence of β-lactam resistance in Strep. uberis, with only one isolate showing resistance to six antimicrobial classes, including cephalosporins.


2021 ◽  
Vol 10 (28) ◽  
Author(s):  
Lucía Fernández ◽  
Ana Catarina Duarte ◽  
Beatriz Martínez ◽  
Ana Rodríguez ◽  
Pilar García

This study reports the draft genome sequences of Staphylococcus aureus V329, a Bap-producing strain isolated from a case of subclinical bovine mastitis in Spain, and a derived mutant (BIM-1) resistant to phage phiIPLA-RODI. Comparison of the two genomes revealed that the mutant strain has a point mutation in the gene tagO .


2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Danielle Mendes Silva ◽  
Mônica Pacheco da Silva ◽  
Pedro M. Pereira Vidigal ◽  
Rafael Mazioli Barcelos ◽  
Raphael Contelli Klein ◽  
...  

Here, we present the draft genome sequences of four Staphylococcus aureus strains isolated from mastitic milk collected from animals with subclinical manifestations. Three of them were typed as sequence type 126 (ST126), a genotype with no genome sequence available. ST126 is found in several herds of southern Brazil and is described as a bovine pathogen strongly associated with milk around the world.


2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Dominik Strapagiel ◽  
Paulina Borówka ◽  
Błażej Marciniak ◽  
Zofia Bakuła ◽  
Jakko van Ingen ◽  
...  

Mycobacterium kansasiibelongs to the nontuberculous mycobacteria (NTM) and causes opportunistic infections with both pulmonary and extrapulmonary manifestations. Here, we report the draft genome sequences of six environmentalM. kansasiistrains, designated 1010001495 (type I), 1010001469 (type II), 1010001468 (type III), 1010001458 (type IV), 1010001454 (type V), and 1010001493 (type V), originally isolated in five different European countries.


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