scholarly journals Genotyping and Antimicrobial Susceptibility Profiling of Streptococcus uberis Isolated from a Clinical Bovine Mastitis Outbreak in a Dairy Farm

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 644
Author(s):  
Valentina Monistero ◽  
Antonio Barberio ◽  
Paola Cremonesi ◽  
Bianca Castiglioni ◽  
Stefano Morandi ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bovine Mastitis ◽  
Dairy Farm ◽  
Clinical Mastitis ◽  
Resistance Rate ◽  
Molecular Characteristics ◽  
Streptococcus Uberis ◽  
Rapd Pcr ◽  
Rapd Pattern ◽  
Low Prevalence

Streptococcus uberis, an environmental pathogen responsible also for contagious transmission, has been increasingly implicated in clinical mastitis (CM) cases in Europe. We described a 4-month epidemiological investigation of Strep. uberis CM cases in an Italian dairy farm. We determined molecular characteristics and phenotypic antimicrobial resistance of 71 Strep. uberis isolates from dairy cows with CM. Genotypic variability was investigated via multiplex PCR of housekeeping and virulence genes, and by RAPD-PCR typing. Antimicrobial susceptibility was assessed for 14 antimicrobials by MIC assay. All the isolates carried the 11 genes investigated. At 90% similarity, two distinct clusters, grouping 69 of the 71 isolates, were detected in the dendrogram derived from the primer ERIC1. The predominant cluster I could be separated into two subclusters, containing 38 and 14 isolates, respectively. Strep. uberis strains belonging to the same RAPD pattern differed in their resistance profiles. Most (97.2%) of them were resistant to at least one of the drugs tested, but only 25.4% showed a multidrug resistance phenotype. The highest resistance rate was observed for lincomycin (93%), followed by tetracycline (85.9%). This study confirmed a low prevalence of β-lactam resistance in Strep. uberis, with only one isolate showing resistance to six antimicrobial classes, including cephalosporins.

scholarly journals Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2595 ◽  
Author(s):  
Rafael Ambrósio Loures ◽  
Ulisses De Pádua Pereira ◽  
Glei Dos Anjos de Carvalho-Castro ◽  
Gláucia Frasnelli Mian ◽  
Dircéia Aparecida da Costa Custódio ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Virulence Genes ◽  
Mammary Epithelial Cells ◽  
Bovine Mastitis ◽  
Mammary Epithelial ◽  
Molecular Characteristics ◽  
Dairy Herds ◽  
High Genetic Diversity ◽  
Streptococcus Uberis ◽  
Intensively Managed

Mastitis is one of the most common and costly infectious diseases in dairy cattle worldwide. This is a multifactorial illness caused by different microorganisms, including virus, yeasts, algae, parasites, and several species of bacteria. Among these bacteria, Streptococcus uberis is an important environmental pathogen that is responsible for a large range of clinical and subclinical mammary infections, especially in intensively managed herds. Despite the increasing importance of this pathogen in the etiology of bovine mastitis, data on its virulence and diversity in Brazilian dairy herds are scarce. The aims of the present study were to investigate the virulence characteristics of S. uberis isolated from bovine mastitis and to assess the molecular epidemiology of the Brazilian isolates using pulsed-field gel electrophoresis (PFGE). In this work, 46 strains of S. uberis isolated from bovine mastitis from 26 Brazilian dairy herds were evaluated regarding their genetic diversity by PFGE using with the SmaI enzyme. Additionally, the presence of the virulence genes skc and pauA, which encode plasminogen activators, and the gene sua, which encodes an adhesion molecule in mammary epithelial cells, were assessed by PCR. Our results showed a high genetic diversity in the population, displaying many different patterns in the PFGE analysis. A high proportion of strains was positive for virulence genes in the sampled population (sua [100%], pauA [91%], and skc [91%]). The high frequency of skc, pauA, and sua genes among the studied strains suggests the importance of these virulence factors, possibly helping S. uberis in the colonization of the bovine mammary gland. Surveys of the genetic and molecular characteristics of this pathogen can improve our knowledge of bacterial activity and identify molecules that have roles in the establishment of the infection. This might help in the development of more effective measures to control and prevent bovine mastitis.

scholarly journals Prevalence of bovine mastitis pathogens and antibioresistance of Escherichia coli isolates

2017 ◽  
Vol 55 (2) ◽  
pp. 113
Author(s):  
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ) ◽  
P. TSAKOS (Π. ΤΣΑΚΟΣ) ◽  
K. ANATOLIOTIS (Κ. ΑΝΑΤΟΛΙΩΤΗΣ)
Keyword(s):  
Escherichia Coli ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Resistance Rate ◽  
Northern Greece ◽  
E Coli ◽  
Milk Samples ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Mastitis Pathogens

Nine hundred and fifty two milk samples from clinical bovine mastitis cases, originated from 269 farms in Northern Greece, were tested. Escherichia coli was isolated in 49.3% of samples. Furthermore, Staphylococcus spp, Corynebacterium spp, Streptococcus spp, Pseudomonas spp, Proteus spp, Klebsiella spp or a combination of the above bacteria were isolated. The bacteriological result from 4.3% of samples was negative. No correlation between clinical mastitis cases and seasonal variation was observed. Resistance of E. coli isolates to tetracycline was 82-96%, to enrofloxacin 10-30%, to gentamicin 58-80%, to cephalosporins 47-75%, to sulfamethoxazole-trimethoprime 46-81%, to ampicillin 71-92% and to neomycin 83-97%. The highest resistance rate and the appearance of multi-resistant isolates of E. coli (6%), to 8 antibacterials, were recorded during the last year of the survey.

scholarly journals Development of an improved Streptococcus uberis experimental mastitis challenge model using different doses and strains in lactating dairy cows

2015 ◽  
Vol 82 (4) ◽  
pp. 470-477 ◽  
Author(s):  
Manouchehr Khazandi ◽  
Patricia Eats ◽  
Darren Trott ◽  
Esmaeil Ebrahimie ◽  
Jeanette Perry ◽  
...  
Keyword(s):  
Success Rate ◽  
Bovine Mastitis ◽  
Clinical Signs ◽  
Clinical Mastitis ◽  
Clinical Findings ◽  
Infection Model ◽  
Treatment Groups ◽  
Streptococcus Uberis ◽  
Reliable Model ◽  
High Doses

Developing a reliable mastitis challenge infection model is required to test new intramammary antimicrobial preparations, other novel bovine mastitis treatments, and study mastitis pathogenesis. Three treatment groups of Holstein Friesian cows in active lactation were administered two doses (104 and 106 cfu/quarter) on a single occasion with one of the three Streptococcus uberis strains (BFR6019, MFF1283 and SA002) suspended in 5 ml of sterile PBS, administered via intramammary inoculation immediately after milking. All quarters that were challenged with S. uberis strains MLF1283 and BFR6019 showed clinical signs of mastitis on day 1 and 2 after the challenge. Strain SA002 had a lower rate of inducing clinical mastitis which was detected later than day 3 after the challenge. We successfully developed a rapid and reliable model for inducing experimental S. uberis mastitis with 100% success rate in cows in active lactation. On the basis of the correlation results between strains, RAPD fingerprinting results, clinical findings, and a 100% success rate of mastitis induction for low and high doses S. uberis strains MLF1283 and BFR6019, strain virulence seems to be a more important effect than challenge dose in induction of clinical mastitis following experimental challenge.

scholarly journals Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ma. Fabiola León-Galván ◽  
José E. Barboza-Corona ◽  
A. Arianna Lechuga-Arana ◽  
Mauricio Valencia-Posadas ◽  
Daniel D. Aguayo ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Bovine Mastitis ◽  
Dairy Herd ◽  
Subclinical Mastitis ◽  
Herd Size ◽  
Clinical Mastitis ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
California Mastitis Test ◽  
Streptococcus Uberis

Thirty-two farms (n=535cows) located in the state of Guanajuato, Mexico, were sampled. Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins ofBacillus thuringiensiswas tested. Forty-six milk samples were selected for their positive California Mastitis Test (CMT) (≥3) and any abnormality in the udder or milk. The frequency of SCM and CLM was 39.1% and 9.3%, respectively. Averages for test day milk yield (MY), lactation number (LN), herd size (HS), and number of days in milk (DM) were 20.6 kg, 2.8 lactations, 16.7 animals, and 164.1 days, respectively. MY was dependent on dairy herd (DH), LN, HS, and DMP<0.01, and correlations between udder quarters from the CMT were around 0.49P<0.01. Coagulase-negative staphylococci were mainly identified, as well asStaphylococcus aureus,Streptococcus uberis,Brevibacterium stationis,B. conglomeratum, andStaphylococcus agnetis. Bacterial isolates were resistant to penicillin, clindamycin, ampicillin, and cefotaxime. Bacteriocins synthesized byBacillus thuringiensisinhibited the growth of multiantibiotic resistance bacteria such asS. agnetis,S. equorum,Streptococcus uberis,Brevibacterium stationis, andBrachybacterium conglomeratum, but they were not active againstS. sciuri, a microorganism that showed an 84% resistance to antibiotics tested in this study.

scholarly journals Molecular Typing and Antimicrobial Susceptibility Profiles of Streptococcus uberis Isolated From Sheep Milk

2021 ◽  
Author(s):  
Nives Maria Rosa ◽  
Ilaria Dupre ◽  
Elisa Azara ◽  
Carla Maria Longheu ◽  
Sebastiana Tola
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Genetic Relatedness ◽  
Efflux Pump ◽  
Resistance Mechanism ◽  
Resistance Rate ◽  
Disk Diffusion Test ◽  
Sheep Milk ◽  
Streptococcus Uberis

Intrammary infections are a major problem for dairy sheep farms, and Streptococcus uberis is one of the main etiological agents of ovine mastitis. Surveys on antimicrobial resistance are still limited in sheep and characterization of isolates is important for acquiring information on resistance and for optimizing therapy. In this study, a sampling of 124 S. uberis isolates collected in Sardinia (Italy) from sheep milk was analysed by multilocus-sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) for genetic relatedness. All isolates were also subjected to antimicrobial susceptibility analysis by the disk diffusion test using a panel of 14 antimicrobials. Resistance genes were detected by PCR assays. MLST analysis revealed that the isolates were grouped into 86 sequence types (STs), of which 73 were new genotypes, indicating a highly diverse population of S. uberis. The most frequently detected lineage was the clonal complex (CC)143, although representing only 13.7% of all characterized isolates. A high level of heterogeneity was also observed among the SmaI PFGE profiles, with 121 unique patterns. Almost all (96.8%) isolates were resistant to at least one antimicrobial, while all exhibited phenotypic susceptibility to oxacillin, amoxicillin-clavulanic acid and ceftiofur. Of the antimicrobials tested, the highest resistance rate was found against streptomycin (93.5%), kanamycin (79.8%) and gentamicin (64.5%), followed by novobiocin (25%) and tetracycline-TE (19.3%). Seventy-four (59.7%) isolates were simultaneously resistant to all aminoglycosides tested. Seventeen isolates (13.7%) exhibited multidrug resistance. All aminoglycosides-resistant isolates were PCR negative for aad-6 and aphA-3’ genes. Among the TE-resistant isolates, the tetM gene was predominant, indicating that the resistance mechanism is mainly mediated by the protection of ribosomes and not through the efflux pump. Three isolates were resistant to erythromycin, and two of them harboured the ermB gene. This is the first study reporting a detailed characterization of the S. uberis strains circulating in Sardinian sheep. Further investigations will be needed to understand the relationships between S. uberis genotypes, mastitis severity, and intra-mammary infection dynamics in the flock, as well as to monitor the evolution of antimicrobial resistance.

scholarly journals Study on Bovine Mastitis under Different Management in Pastoral and Agro-Pastoral Areas of Borana Zone, Southern Ethiopia

2020 ◽  
Vol 5 (1) ◽  
pp. 1-8
Author(s):  
Adem Kumbe
Keyword(s):  
Risk Factors ◽  
Culture Media ◽  
Bovine Mastitis ◽  
Dairy Farm ◽  
Clinical Mastitis ◽  
Southern Ethiopia ◽  
Coagulase Negative Staphylococcus ◽  
Cross Sectional ◽  
California Mastitis Test ◽  
Borana Zone

A cross-sectional study was conducted to determine the prevalence of bovine mastitis, associated potential risk factors and major etiological agents of clinical and sub clinical mastitis under different management condition of pastoral, agro-pastoral, ranch and farm by using California mastitis test and bacteriology. A total of 384 lactating Borana cows in Did-tuyura ranch, Yabello Pastoral and dryland agriculture research center (YPDARC) dairy farm and three districts namely Gomole, Moyale and Yabello of Borana zone were included in the study. The study revealed that overall prevalence of mastitis were 47.4 % (182/384); out of which 12 % (46/384) clinical and 35.4 % (136/384) sub-clinical mastitis whereas prevalence at quarter level was 21.48% (330/1536) of which 3% (46/1536) and 18.48% (284/1536) were clinical and sub-clinical form respectively. From the total examined quarter, 3.5% (53) of quarters had blind quarter. Prevalence in pastoral and agro-pastoral herding system (extensive management system) at cow level and quarter level were 18.9% and 10.9% respectively while prevalence in Did-tuyura ranch and YPDARC dairy farm herding system (semi-intensive) were 20% and 7% at cow level and quarter level respectively. The prevalence of mastitis significantly (P<0.05) differed with parity, stage of lactation and body condition of lactating animals. From 330 California Mastitis Test (CMT) and clinically positive milk samples there was growth of bacteria on culture media observed only in 155 (46.97%). Out of this Staphylococcus aureus accounted for 59 (38.06%) isolates followed by Streptococcus species 33 (21.29%) and Coagulase negative Staphylococcus 30 (19.35%). Due to lack of proper managements of different risk factors major pathogenic microorganisms are isolated. Proper preventive and control strategy, awareness creation on key factors of mastitis, Regular screening and culling of chronically infected cows should be practiced.

scholarly journals Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2595
Author(s):  
Rafael Ambrósio Loures ◽  
Ulisses De Pádua Pereira ◽  
Glei Dos Anjos de Carvalho-Castro ◽  
Gláucia Frasnelli Mian ◽  
Dircéia Aparecida da Costa Custódio ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Virulence Genes ◽  
Mammary Epithelial Cells ◽  
Bovine Mastitis ◽  
Mammary Epithelial ◽  
Molecular Characteristics ◽  
Dairy Herds ◽  
High Genetic Diversity ◽  
Streptococcus Uberis ◽  
Intensively Managed

Mastitis is one of the most common and costly infectious diseases in dairy cattle worldwide. This is a multifactorial illness caused by different microorganisms, including virus, yeasts, algae, parasites, and several species of bacteria. Among these bacteria, Streptococcus uberis is an important environmental pathogen that is responsible for a large range of clinical and subclinical mammary infections, especially in intensively managed herds. Despite the increasing importance of this pathogen in the etiology of bovine mastitis, data on its virulence and diversity in Brazilian dairy herds are scarce. The aims of the present study were to investigate the virulence characteristics of S. uberis isolated from bovine mastitis and to assess the molecular epidemiology of the Brazilian isolates using pulsed-field gel electrophoresis (PFGE). In this work, 46 strains of S. uberis isolated from bovine mastitis from 26 Brazilian dairy herds were evaluated regarding their genetic diversity by PFGE using with the SmaI enzyme. Additionally, the presence of the virulence genes skc and pauA, which encode plasminogen activators, and the gene sua, which encodes an adhesion molecule in mammary epithelial cells, were assessed by PCR. Our results showed a high genetic diversity in the population, displaying many different patterns in the PFGE analysis. A high proportion of strains was positive for virulence genes in the sampled population (sua [100%], pauA [91%], and skc [91%]). The high frequency of skc, pauA, and sua genes among the studied strains suggests the importance of these virulence factors, possibly helping S. uberis in the colonization of the bovine mammary gland. Surveys of the genetic and molecular characteristics of this pathogen can improve our knowledge of bacterial activity and identify molecules that have roles in the establishment of the infection. This might help in the development of more effective measures to control and prevent bovine mastitis.

scholarly journals Genome Sequences of Klebsiella variicola Isolates from Dairy Animals with Bovine Mastitis from Newfoundland, Canada

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Fraser W. Davidson ◽  
Hugh G. Whitney ◽  
Kapil Tahlan
Keyword(s):  
Opportunistic Infections ◽  
Draft Genome ◽  
Bovine Mastitis ◽  
Dairy Farm ◽  
Clinical Mastitis ◽  
Genome Sequences ◽  
Dairy Animals

Klebsiella variicola was recently reported as an emerging and/or previously misidentified species associated with opportunistic infections in humans. Here, we report the draft genome sequences of K. variicola isolates from two animals with clinical mastitis from a dairy farm in Newfoundland, Canada.

scholarly journals Molecular Typing and Antimicrobial Susceptibility Profiles of Streptococcus uberis Isolated from Sheep Milk

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1489
Author(s):  
Nives Maria Rosa ◽  
Ilaria Duprè ◽  
Elisa Azara ◽  
Carla Maria Longheu ◽  
Sebastiana Tola
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Genetic Relatedness ◽  
Efflux Pump ◽  
Resistance Mechanism ◽  
Resistance Rate ◽  
Disk Diffusion Test ◽  
Sheep Milk ◽  
Streptococcus Uberis

Intramammary infections are a major problem for dairy sheep farms, and Streptococcus uberis is one of the main etiological agents of ovine mastitis. Surveys on antimicrobial resistance are still limited in sheep and characterization of isolates is important for acquiring information on resistance and for optimizing therapy. In this study, a sampling of 124 S. uberis isolates collected in Sardinia (Italy) from sheep milk was analyzed by multilocus-sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) for genetic relatedness. All isolates were also subjected to antimicrobial susceptibility analysis by the disk diffusion test using a panel of 14 antimicrobials. Resistance genes were detected by PCR assays. MLST analysis revealed that the isolates were grouped into 86 sequence types (STs), of which 73 were new genotypes, indicating a highly diverse population of S. uberis. The most frequently detected lineage was the clonal complex (CC)143, although representing only 13.7% of all characterized isolates. A high level of heterogeneity was also observed among the SmaI PFGE profiles, with 121 unique patterns. Almost all (96.8%) isolates were resistant to at least one antimicrobial, while all exhibited phenotypic susceptibility to oxacillin, amoxicillin-clavulanic acid and ceftiofur. Of the antimicrobials tested, the highest resistance rate was found against streptomycin (93.5%), kanamycin (79.8%) and gentamicin (64.5%), followed by novobiocin (25%) and tetracycline-TE (19.3%). Seventy-four (59.7%) isolates were simultaneously resistant to all aminoglycosides tested. Seventeen isolates (13.7%) exhibited multidrug resistance. All aminoglycosides-resistant isolates were PCR negative for aad-6 and aphA-3′ genes. Among the TE-resistant isolates, the tetM gene was predominant, indicating that the resistance mechanism is mainly mediated by the protection of ribosomes and not through the efflux pump. Three isolates were resistant to erythromycin, and two of them harbored the ermB gene. This is the first study reporting a detailed characterization of the S. uberis strains circulating in Sardinian sheep. Further investigations will be needed to understand the relationships between S. uberis genotypes, mastitis severity, and intra-mammary infection dynamics in the flock, as well as to monitor the evolution of antimicrobial resistance.

scholarly journals Bacteria from Bovine Clinical Mastitis Showed Multiple Drug Resistance

2021 ◽  
Author(s):  
Sudhakar P Awandkar ◽  
Mahesh B Kulkarni
Keyword(s):  
Public Health ◽  
Multiple Drug Resistance ◽  
Bacterial Species ◽  
Bovine Mastitis ◽  
Dairy Industry ◽  
Economic Loss ◽  
Clinical Mastitis ◽  
Multiple Drug ◽  
Gram Negative ◽  
Streptococcus Uberis

Abstract Mastitis, most often udder infection of dairy animals attracted concerns due to heavy economic loss to dairy industry and public health. This study was conducted to determine the cultivable bacterial species associated with bovine clinical mastitis and their resistance patterns to different antimicrobials. The milk samples from 272 quarters of cows suffering from clinical mastitis were investigated. A total of 110 bacterial isolates belonging to 14 different genera were isolated and identified. Aminoglycosides and Quinolones were found to be most effective antibiotics. We demonstrated Extended Spectrum β Lactamases (ESBL), Cephalosporins, Tetracyclines, Vancomycin and Chloramphenicol resistant Gram-positive and Gram-negative bacteria along with Vancomycin Resistant Enterococci (VRE), Multiple Drug Resistant Gram-Negative Rods (MDR-GNR), MDR-Pseudomonas (MDR-P) and MDR Acinetobacter (MDR-A). The ESBLs and cephalosporins resistant S. aureus isolates showed resistance to Vancomycin. Wide spread of resistance among Streptococcus uberis against ESBLs and Cephalosporins, widely used antibiotics in bovine mastitis, was documented. Variable MDR patterns were recorded for every species. MDR transfer from non-pathogens to emerging foodborne and established mastitis pathogens could be potential problem to dairy industry as well as public health.

Sign in / Sign up

Export Citation Format

Share Document