Cryptococcus gattii Infection Dampens Th1 and Th17 Responses by Attenuating Dendritic Cell Function and Pulmonary Chemokine Expression in the Immunocompetent Hosts
ABSTRACTCryptococcal infections are primarily caused by two related fungal species:Cryptococcus neoformansandCryptococcus gattii. It is well known thatC. neoformansgenerally affects immunocompromised hosts; however,C. gattiiinfection can cause diseases in not only immunocompromised hosts but also immunocompetent individuals. While recent studies suggest thatC. gattiiinfection could dampen pulmonary neutrophil recruitment and inflammatory cytokine production in immunocompetent hosts, the impact ofC. gattiiinfection on the development of their adaptive T helper cell immune response has not been addressed. Here, we report thatC. neoformansinfection with highly virulent and less virulent strains preferentially induced pulmonary Th1 and Th17 immune responses in the host, respectively. However, fewer pulmonary Th1 and Th17 cells could be detected in mice infected withC. gattiistrains. Notably, dendritic cells (DC) in mice infected withC. gattiiexpressed much lower levels of surface MHC-II andIl12orIl23transcripts and failed to induce effective Th1 and Th17 differentiationin vitro. Furthermore, the expression levels ofIp10andCxcl9transcripts, encoding Th1-attracting chemokines, were significantly reduced in the lungs of mice infected with the highly virulentC. gattiistrain. Thus, our data suggest thatC. gattiiinfection dampens the DC-mediated effective Th1/Th17 immune responses and downregulates the pulmonary chemokine expression, thus resulting in the inability to mount protective immunity in immunocompetent hosts.