scholarly journals Microtitration agglutination for detection of Treponema hyodysenteriae antibody

1978 ◽  
Vol 8 (3) ◽  
pp. 293-298
Author(s):  
L A Joens ◽  
D L Harris ◽  
J M Kinyon ◽  
M L Kaeberle

A microtitration agglutination test for the detection of Treponema hyodysenteriae antibody in swine and rabbit sera is described. The following methods provided the best test results: antigen produced from the spirochete after a culturing period of 36 to 44 h at 38 degrees C, washed antigen inactivated with 0.01% Merthiolate at 4 degrees C for 24 to 36 h, sera heated at 56 degrees C for 30 min, a diluent of phosphate-buffered saline (0.01 M, pH 7.2), and test results read macroscopically after 18 to 24 h of incubation at 38 degrees C. The test enabled detection of antibody against pathogenic T. hyodysenteriae with a high level of consistency and sensitivity. Sera against nonpathogenic T. hyodysenteriae produced low agglutinating titers (less than or equal to 1:8) when reacted against antigen from pathogenic isolates. Inactivated antigen remained stable for 7 to 10 days. Specificity of the reaction in the agglutination test was shown by absorption studies.

Author(s):  
Ni Putu Dian Permata Prasetyaningrum

Surabaya Shipping Polytechnic emphasizes on certain areas of expertise that Taruna must possess. This is the basis after graduating from shipping polytechnics, cadets must have expertise and skills. The purpose of this study was to study the effect of inquiry, discovery learning, and creativity levels on the ability to write descriptive essays on nautical and technical cadets at Surabaya Shipping Polytechnic. This type of research is research. This research uses quantitative methods using experiments. The location used in this research is Surabaya Shipping Polytechnic. The subjects in this study were the cadets of the Nautika A, Nautika B, Teknika A, and Teknika B. classes. Based on the results of the research and discussion, the following conclusions are obtained: There are those that can be solved looking for description essays in the cadets. learning discovery method. The test results show better investigation methods than the discovery of learning, There is a difference in the ability to write a description essay about cadets who have a high level of creativity with cadets who have a low level of creativity, the test results show better who have a high level of creativity, there are related with learning methods and descriptions of the ability to write essay descriptions, the test results show learning methods and creativity descriptions of the ability to write essay descriptions.


2021 ◽  
Vol 11 (2) ◽  
pp. 75
Author(s):  
Jan Amos Jelinek

The Earth’s shape concept develops as consecutive cognitive problems (e.g., the location of people and trees on the spherical Earth) are gradually resolved. Establishing the order of problem solving may be important for the organisation of teaching situations. This study attempted to determine the sequence of problems to be resolved based on tasks included in the EARTH2 test. The study covered a group of 444 children between 5 and 10 years of age. It captured the order in which children solve cognitive problems on the way to constructing a science-like concept. The test results were compared with previous studies. The importance of cultural influences connected to significant differences (24%) in test results was emphasised. Attention was drawn to the problem of the consistency of the mental model approach highlighted in the literature. The analysis of the individual sets of answers provided a high level of consistency of indications referring to the same model (36%), emphasising the importance of the concept of mental models.


Metals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1100
Author(s):  
Alexandre M. Emelyanenko ◽  
Valery V. Kaminsky ◽  
Ivan S. Pytskii ◽  
Kirill A. Emelyanenko ◽  
Alexander G. Domantovsky ◽  
...  

The interest in magnesium-based materials is promoted by their biocompatibility, their bioresorbability, and their recently discovered antibacterial potential. Until now, the widespread use of magnesium alloys in different corrosive environments was inhibited by their weakly controllable degradation rate and poorly understood microbiologically induced corrosion behavior. To better understand the degradation and usability of magnesium-based alloys, in this study we have fabricated superhydrophobic coatings on a magnesium-based alloy, and analyzed the behavior of this alloy in bacterial dispersions of Pseudomonas aeruginosa and Klebsiella pneumoniae cells in phosphate-buffered saline. It was shown that the immersion of such coatings in bacterial dispersions causes notable changes in the morphology of the samples, dependent on the bacterial dispersion composition and the type of bacterial strain. The interaction of the superhydrophobic coatings with the bacterial dispersion caused the formation of biofilms and sodium polyphosphate films, which provided enhanced barrier properties in magnesium dissolution and hence in dispersion medium alkalization, eventually leading to the inhibition of magnesium substrate degradation. The electrochemical data obtained for superhydrophobic samples in continuous contact with corrosive bacterial dispersions for 48 h indicated a high level of anticorrosion protection.


Information ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Alexey Semenkov ◽  
Dmitry Bragin ◽  
Yakov Usoltsev ◽  
Anton Konev ◽  
Evgeny Kostuchenko

Modern facial recognition algorithms make it possible to identify system users by their appearance with a high level of accuracy. In such cases, an image of the user’s face is converted to parameters that later are used in a recognition process. On the other hand, the obtained parameters can be used as data for pseudo-random number generators. However, the closeness of the sequence generated by such a generator to a truly random one is questionable. This paper proposes a system which is able to authenticate users by their face, and generate pseudo-random values based on the facial image that will later serve to generate an encryption key. The generator of a random value was tested with the NIST Statistical Test Suite. The subsystem of image recognition was also tested under various conditions of taking the image. The test results of the random value generator show a satisfactory level of randomness, i.e., an average of 0.47 random generation (NIST test), with 95% accuracy of the system as a whole.


2011 ◽  
Vol 261-263 ◽  
pp. 989-993 ◽  
Author(s):  
Anuchit Uchaipichat ◽  
Ekachai Man Koksung

An experimental program of laboratory bearing tests was performed to characterize the bearing capacity of foundation on unsaturated granular soils. All tests were performed by pushing a circular rod on the surface of compacted sand specimens with different values of matric suction until failure. The test results show an increase in ultimate bearing capacity with increasing matric suction at low suction value but a decrease in that at high level of suction. The comparisons between the test results and simulations using the expressions proposed in this paper are presented and discussed. Good agreements are achieved for all testing values of suction.


Author(s):  
Md Tanzil Ansari ◽  
Sukumar Ghosh ◽  
Shailendra Kumar Singh

Nowadays, people are more vulnerable to metabolic disorders due to their faulty dietary and behavioural habits. One such disorder is Vatarakta which causes functional impairment due to involvement of Sandhi (joints). It is manifested by Ruk, Toda, Sparsha asahatva, Shopha, Raga, Daha and Stabdhata in Sandhi. Vatarakta can be correlated with Hyperuricaemia or Gout due to similarity in their clinical features. Hyperuricaemia is defined as abnormally high level of uric acid in blood (i.e. >6mg/dl in female and >7mg/dl in male). On the other hand, Gout is an inflammatory response to monosodium urate crystals formed secondary to hyperuricaemia. Aims and objectives: 1. To evaluate the effectiveness of Trikarshika kwatha and lifestyle modification in the management of Vatarakta. 2. To compare the effects of Trikarshika kwatha with and without lifestyle modification in the management of Vatarakta. Materials and methods: Raw herbs of the research formulation were collected after proper identification and Kwatha was prepared for oral administration. For the clinical study, total 60 patients were selected on the basis of selection criteria. Selected patients were randomly divided into two groups. (i) Group A: 30 patients were treated with Trikarshika kwatha. (ii) Group B: 30 patients were treated with Trikarshika kwatha along with Lifestyle modification. Individual patient was treated for 45 days along with follow up at the interval of every 15 days. To assess the effectiveness of treatment, scoring pattern was followed for subjective and objective parameters. They were assessed before and after treatment. The collected data were analysed statistically by using Paired t-test. Results: On the basis of all statistical data, it can be said that patients of Group B showed better results in all parameters in comparison to patients of Group A. Conclusion: Both Trikarshika kwatha and Lifestyle modification are affective but Trikarshika kwatha with Lifestyle modification is more effective than Trikarshika kwatha without Lifestyle modification in the management of Vatarakta.


2020 ◽  
Vol 21 (6) ◽  
Author(s):  
Alimohammad Bananzadeh ◽  
Seyed Vahid Hosseini ◽  
Hajar Khazraei ◽  
Mohammad Mehdi Lashkarizadeh ◽  
Leila Ghahramani ◽  
...  

Background: Bariatric surgery has resulted in body weight loss, which claimed by surgery removal specific parts of the stomach with enzyme or sleeve gastrectomy. Objectives: The aim of this study is to determine weight loss and endocrine changes by 12-week fundus resection and sleeve gastrectomy in rabbits. Methods: Twenty-one rabbits, weighing 2.5 - 3.5 kg, were divided into three groups (n = 7): sleeve gastrectomy, experimental fundus resection, and sham group. The weight of rabbits and total ghrelin and leptin levels in the plasma before and after surgery were measured in 12 weeks. Statistical analyses were performed using the Kruskal-Walis test for comparison of the means between the groups, and the difference after months in one group was assayed by Friedman test. Results: The results showed sleeve gastrectomy had a significant weight loss after one month when compared to fundus resection and sham-operated controls (P = 0.008). There was no significant difference in the ghrelin levels after these surgeries, but leptin levels decreased significantly after the fundectomy (P = 0.025). Conclusions: Sleeve gastrectomy is more efficient than the fundus resection in weight loss. It could be suggested as a new option in metabolic disorders due to the high level of leptin.


Author(s):  
N. Sandhya Rani ◽  
M. Sarada Devi

Empowerment of tribal women is one of the central issues in the process of development all over the world. Empowerment is the process that allows one to gain the knowledge and attitude needed to cope with the changing world and the circumstances in which one lives [1]. Women empowerment is a process in which women gain greater share of control over material, human and intellectual resources as well as control over decision-making in their home, community, society and nation. Given the need to analyze the empowerment status of tribal women, the present study aimed to enhance the empowerment status through enhancing decision-making skills of tribal working women in India. The specific objective is to study the impact of intervention on enhancing status of empowerment through decision-making skills of tribal working women in Utnoor Mandal Adilabad district. The total sample population for the study was 50 tribal working women, and data was analyzed using a paired t test. Results revealed that at pretest, majority of the women were at average level of decision-making skills (78%), 12% were at low level and only 10% were at high level. After the intervention, post test results revealed that 74% of the women were high in decision making skills and remaining 26% were at average level. Interestingly, none of the respondents had low level of life skills. Thus, intervention found to be effective among women respondents to develop and enhance their empowerment status through decision-making skills.


1932 ◽  
Vol 5 (3-4) ◽  
pp. 73-130

Value of Agglutination-Test. - Der Wert der Agglutinations Methode bei der weissen Ruhr. Dr. Sachweh, München. Berliner Tierärztliche Wochenschrift, 1931, p. 845.A number of chickens were subjected, every three months, to the agglutination test. During this test it appeared that the agglutination titer varied considerably. All animals, of which a great number reacted positively at the beginning of the test, reacted negatively at the end thereof. This made it possible to comprehend the strongly varying results of the different tests. A group of 400 positive reacting hens gave healthy chicks. The wide advertising of the rapid blood test results in the poultry farmers making their own researches, and owing to the varying results obtained, it forces them to attach no value thereto.


2005 ◽  
Vol 11 (1) ◽  
pp. 3-14 ◽  
Author(s):  
David Powis ◽  
Miles Bore ◽  
Donald Munro ◽  
Mary Ann Lumsden

A review of the medical student selection literature and our own past research (Lowe, Kerridge, Bore, Munro and Powis (2001) has indicated that competent and ethical practice of medicine requires doctors to possess a range of personal qualities in addition to high-level academic ability. A three-part test battery called the Personal Qualities Assessment (PQA) has been developed as a measure of some of these qualities: it consists of the Mental Agility Test (MAT), which measures cognitive skills, the Mojac scale, which measures moral orientation, and the NACE scale, which measures Narcissism, Aloofness, Confidence and Empathy. Five hundred and seven applicants for entry in October 2003 to the Scottish medical schools volunteered to complete the MAT, Mojac and NACE tests in January 2003. The test results played no part in making or informing selection decisions. The scores obtained by the candidates on each test covered a wide range, indicating that each test component has good discriminating power. Correlations between the test components were low (−0.02 to +0.17), indicating that they each measure different attributes. Since the test results were not used in making selection decisions it will be possible to relate outcome indices (e.g., examination and professional performance as the students progress through medical school) to the test component scores to seek evidence for the predictive validity of the PQA battery and thereby indicate its potential usefulness as a selection tool.


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