scholarly journals Clustering and Erratic Movement Patterns of Syringe-Injected versus Mosquito-Inoculated Malaria Sporozoites Underlie Decreased Infectivity

mSphere ◽  
2021 ◽  
Vol 6 (2) ◽  
Author(s):  
C. M. de Korne ◽  
B. M. F. Winkel ◽  
M. N. van Oosterom ◽  
S. Chevalley-Maurel ◽  
H. M. Houwing ◽  
...  
Keyword(s):  
Movement Patterns ◽  
Movement Pattern ◽  
Host Liver ◽  
Content Type ◽  
Vaccine Candidates ◽  
Administration Routes ◽  
Dermal Tissue ◽  
Tissue Morphology ◽  
Automated Imaging ◽  
Syringe Injection

ABSTRACT Malaria vaccine candidates based on live, attenuated sporozoites have led to high levels of protection. However, their efficacy critically depends on the sporozoites’ ability to reach and infect the host liver. Administration via mosquito inoculation is by far the most potent method for inducing immunity but highly impractical. Here, we observed that intradermal syringe-injected Plasmodium berghei sporozoites (syrSPZ) were 3-fold less efficient in migrating to and infecting mouse liver than mosquito-inoculated sporozoites (msqSPZ). This was related to a clustered dermal distribution (2-fold-decreased median distance between syrSPZ and msqSPZ) and, more importantly, a 1.4-fold (significantly)-slower and more erratic movement pattern. These erratic movement patterns were likely caused by alteration of dermal tissue morphology (>15-μm intercellular gaps) due to injection of fluid and may critically decrease sporozoite infectivity. These results suggest that novel microvolume-based administration technologies hold promise for replicating the success of mosquito-inoculated live, attenuated sporozoite vaccines. IMPORTANCE Malaria still causes a major burden on global health and the economy. The efficacy of live, attenuated malaria sporozoites as vaccine candidates critically depends on their ability to migrate to and infect the host liver. This work sheds light on the effect of different administration routes on sporozoite migration. We show that the delivery of sporozoites via mosquito inoculation is more efficient than syringe injection; however, this route of administration is highly impractical for vaccine purposes. Using confocal microscopy and automated imaging software, we demonstrate that syringe-injected sporozoites do cluster, move more slowly, and display more erratic movement due to alterations in tissue morphology. These findings indicate that microneedle-based engineering solutions hold promise for replicating the success of mosquito-inoculated live, attenuated sporozoite vaccines.

scholarly journals Clustering and erratic movement patterns of syringe-injected versus mosquito-inoculated malaria sporozoites underlie decreased infectivity

2020 ◽  
Author(s):  
C.M. de Korne ◽  
B.M.F. Winkel ◽  
M.N. van Oosterom ◽  
S. Chevalley-Maurel ◽  
H.M. Houwing ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Mouse Liver ◽  
Injection Pressure ◽  
Movement Patterns ◽  
Movement Pattern ◽  
Host Liver ◽  
Vaccine Candidates ◽  
Dermal Tissue ◽  
Median Distance ◽  
Intercellular Gaps

ABSTRACTLive attenuated malaria sporozoites are promising vaccine candidates, however, their efficacy critically depends on their capability to reach and infect the host liver. Administration via mosquito inoculation is by far the most potent method for inducing immunity, but highly unpractical. Here, we observed that intradermal syringe-injected Plasmodium berghei sporozoites (syrSPZ) were three-fold less efficient in migrating to and infecting mouse liver compared to mosquito-inoculated sporozoites (msqSPZ). This was related to a clustered dermal distribution (2-fold decreased median distance between syrSPZ vs msqSPZ) and, more importantly, a 1.4-fold significantly slower and more erratic movement pattern. These erratic movement patterns were likely caused by alteration of dermal tissue morphology (>15 μm intercellular gaps) due to injection pressure and may critically decrease sporozoite infectivity. These results suggest that novel microvolume-based administration technologies hold promise for replicating the success of mosquito-inoculated live attenuated sporozoite vaccines.

What is the Biomechanical Principle of Forehand Smash in Badminton? - Article Review

2020 ◽  
Vol 7 (2) ◽  
pp. 221-229
Author(s):  
Gilang Estina Putra ◽  
Ria Lumintuarso
Keyword(s):  
Movement Patterns ◽  
Movement Pattern ◽  
Human Movement ◽  
Biomechanical Analysis ◽  
Future Research ◽  
Good Movement

SummaryThe pattern of human movement is strongly influenced by biomotor conditions, age development will affect the level of biomotor maturity. Forehand smash is one of the techniques that every badminton athlete must master well. To do this stroke well, a good movement pattern is needed, biomotor maturity which in this study is related to the age of the athlete. So that the development of age will cause differences in movement patterns which in this study analyzes the forehand smash movement patterns in badminton based on age. It is known that the most efficient badminton technique for gaining points in badminton is the smash. The smash-hit is more likely to produce a fast shuttle lock rate performed using the forehand smash technique. So that in this study the writer will analyze a technique in badminton, namely the forehand smash technique. These findings contribute to future research on the biomechanical analysis of forehand smash motion in badminton.

scholarly journals Movement Patterns of Wintering Grassland Sparrows in Arizona

The Auk ◽  
2000 ◽  
Vol 117 (3) ◽  
pp. 748-759 ◽  
Author(s):  
Caleb E. Gordon
Keyword(s):  
Large Scale ◽  
Radio Telemetry ◽  
Movement Patterns ◽  
Movement Pattern ◽  
Summer Rainfall ◽  
Movement Behavior ◽  
Home Ranges ◽  
Mark Recapture ◽  
Mobile Species ◽  
Study Sites

Abstract I used mark-recapture analysis and radio telemetry to characterize winter movement patterns of six grassland sparrows in southeastern Arizona. Mark-recapture data were generated by banding birds captured during repeated flush-netting sessions conducted on a series of 7-ha plots over three consecutive winters. This resulted in 2,641 captures of 2,006 individual sparrows of the six species. Radio telemetry was conducted concurrently on 20 individuals of four of these species. Recapture data and radio telemetry indicated that Cassin's Sparrow (Aimophila cassinii) and Grasshopper Sparrow (Ammodramus savannarum) were the most sedentary, followed by Baird's Sparrow (Ammodramus bairdii), Vesper Sparrow (Pooecetes gramineus), Savannah Sparrow (Passerculus sandwichensis), and Brewer's Sparrow (Spizella breweri). Grasshopper, Baird's, Savannah, and Vesper sparrows tended to remain within fixed home ranges during winter. With the exception of Savannah Sparrows, whose movement behavior varied among study sites, movement patterns remained constant within species across years and study sites despite radical fluctuations in the absolute and relative abundances of all species. Interspecific differences in movement pattern suggest that species in this system partition niche space according to the regional-coexistence mechanism. Abundances of the most sedentary species, Cassin's, Grasshopper, and Baird's sparrows, were poorly or negatively correlated with summer rainfall at the between-year landscape scale, whereas abundances of the more mobile Savannah, Vesper, and Brewer's sparrows were strongly positively correlated. This is consistent with the theoretical prediction that movement constrains large-scale habitat selection, favoring mobile species in fragmented environments.

scholarly journals Location-Based Tracking Data and Customer Movement Pattern Analysis Using for Sustainable Fashion Business

2019 ◽  
Vol 11 (22) ◽  
pp. 6209 ◽  
Author(s):  
Jonghyuk Kim ◽  
Hyunwoo Hwangbo ◽  
Sung Jun Kim ◽  
Soyean Kim
Keyword(s):  
Field Experiments ◽  
Pattern Analysis ◽  
Movement Patterns ◽  
Movement Pattern ◽  
Tracking Data ◽  
Discrete Location ◽  
Practical Applications ◽  
Before And After ◽  
Stay Time ◽  
Visual Merchandising

Retailers need accurate movement pattern analysis of human-tracking data to maximize the space performance of their stores and to improve the sustainability of their business. However, researchers struggle to precisely measure customers’ movement patterns and their relationships with sales. In this research, we adopt indoor positioning technology, including wireless sensor devices and fingerprinting techniques, to track customers’ movement patterns in a fashion retail store over four months. Specifically, we conducted three field experiments in three different timeframes. In each experiment, we rearranged one element of the visual merchandising display (VMD) to track and compare customer movement patterns before and after the rearrangement. For the analysis, we connected customers’ discrete location data to identify meaningful patterns in customers’ movements. We also used customers’ location and time information to match identified movement pattern data with sales data. After classifying individuals’ movements by time and sequences, we found that stay time in a particular zone had a greater impact on sales than the total stay time in the store. These results challenge previous findings in the literature that suggest that the longer customers stayed in a store, the more they purchase. Further, the results confirmed that effective store rearrangement could change not only customer movement patterns but also overall sales of store zones. This research can be a foundation for various practical applications of tracking data technologies.

scholarly journals Staphylococcus aureus Induces Hypoxia and Cellular Damage in Porcine Dermal Explants

2015 ◽  
Vol 83 (6) ◽  
pp. 2531-2541 ◽  
Author(s):  
Abdul G. Lone ◽  
Erhan Atci ◽  
Ryan Renslow ◽  
Haluk Beyenal ◽  
Susan Noh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Death ◽  
Effective Diffusion ◽  
Oxygen Demand ◽  
Shotgun Proteomics ◽  
Cellular Damage ◽  
Label Free ◽  
Content Type ◽  
Dermal Tissue ◽  
The Difference

We developed a porcine dermal explant model to determine the extent to whichStaphylococcus aureusbiofilm communities deplete oxygen, change pH, and produce damage in underlying tissue. Microelectrode measurements demonstrated that dissolved oxygen (DO) in biofilm-free dermal tissue was 4.45 ± 1.17 mg/liter, while DO levels for biofilm-infected tissue declined sharply from the surface, with no measurable oxygen detectable in the underlying dermal tissue. Magnetic resonance imaging demonstrated that biofilm-free dermal tissue had a significantly lower relative effective diffusion coefficient (0.26 ± 0.09 to 0.30 ± 0.12) than biofilm-infected dermal tissue (0.40 ± 0.12 to 0.48 ± 0.12;P< 0.0001). Thus, the difference in DO level was attributable to biofilm-induced oxygen demand rather than changes in oxygen diffusivity. Microelectrode measures showed that pH within biofilm-infected explants was more alkaline than in biofilm-free explants (8.0 ± 0.17 versus 7.5 ± 0.15, respectively;P< 0.002). Cellular and nuclear details were lost in the infected explants, consistent with cell death. Quantitative label-free shotgun proteomics demonstrated that both proapoptotic programmed cell death protein 5 and antiapoptotic macrophage migration inhibitory factor accumulated in the infected-explant spent medium, compared with uninfected-explant spent media (1,351-fold and 58-fold, respectively), consistent with the cooccurrence of apoptosis and necrosis in the explants. Biofilm-origin proteins reflected an extracellular matrix-adapted lifestyle ofS. aureus. S. aureusbiofilms deplete oxygen, increase pH, and induce cell death, all factors that contribute to impede wound healing.

scholarly journals Identification and Immunological Characterization of Three Potential Vaccinogens against Cryptosporidium Species

2011 ◽  
Vol 18 (11) ◽  
pp. 1796-1802 ◽  
Author(s):  
Patricio A. Manque ◽  
Fernando Tenjo ◽  
Ute Woehlbier ◽  
Ana M. Lara ◽  
Myrna G. Serrano ◽  
...  
Keyword(s):  
The Novel ◽  
Cryptosporidium Hominis ◽  
Content Type ◽  
Vaccine Candidates ◽  
Novel Approach ◽  
Intellectual Abilities ◽  
Significant Impairment ◽  
Life Threatening ◽  
Potential Vaccine ◽  
Cellular Immune

ABSTRACTCryptosporidiosis is a ubiquitous infectious disease, caused by the protozoan parasitesCryptosporidium hominisandCryptosporidium parvum, leading to acute, persistent, and chronic diarrhea with life-threatening consequences in immunocompromised individuals. In developing countries, cryptosporidiosis in early childhood has been associated with subsequent significant impairment in growth, physical fitness, and intellectual abilities. Currently, vaccines are unavailable and chemotherapeutics are toxic and impractical, and agents for immunoprophylaxis or treatment of cryptosporidiosis are a high priority. Availability of the genome sequences forC. hominisandC. parvumprovides new opportunities to procure and examine novel vaccine candidates. Using the novel approach of “reverse vaccinology,” we identified several new potential vaccine candidates. Three of these antigens—Cp15, profilin, and aCryptosporidiumapyrase—were delivered in heterologous prime-boost regimens as fusions with cytolysin A (ClyA) in aSalmonellalive vaccine vector and as purified recombinant antigens, and they were found to induce specific and potent humoral and cellular immune responses, suggesting their potential as new vaccinogens againstCryptosporidiuminfection.

scholarly journals How superdiffusion gets arrested: ecological encounters explain shift from Lévy to Brownian movement

2014 ◽  
Vol 281 (1774) ◽  
pp. 20132605 ◽  
Author(s):  
Monique de Jager ◽  
Frederic Bartumeus ◽  
Andrea Kölzsch ◽  
Franz J. Weissing ◽  
Geerten M. Hengeveld ◽  
...  
Keyword(s):  
Brownian Motion ◽  
Empirical Studies ◽  
Movement Patterns ◽  
Movement Pattern ◽  
Mechanistic Explanation ◽  
Theoretical Argument ◽  
Ecological Interactions ◽  
High Resource ◽  
Dependent Change ◽  
Movement Strategy

Ecological theory uses Brownian motion as a default template for describing ecological movement, despite limited mechanistic underpinning. The generality of Brownian motion has recently been challenged by empirical studies that highlight alternative movement patterns of animals, especially when foraging in resource-poor environments. Yet, empirical studies reveal animals moving in a Brownian fashion when resources are abundant. We demonstrate that Einstein's original theory of collision-induced Brownian motion in physics provides a parsimonious, mechanistic explanation for these observations. Here, Brownian motion results from frequent encounters between organisms in dense environments. In density-controlled experiments, movement patterns of mussels shifted from Lévy towards Brownian motion with increasing density. When the analysis was restricted to moves not truncated by encounters, this shift did not occur. Using a theoretical argument, we explain that any movement pattern approximates Brownian motion at high-resource densities, provided that movement is interrupted upon encounters. Hence, the observed shift to Brownian motion does not indicate a density-dependent change in movement strategy but rather results from frequent collisions. Our results emphasize the need for a more mechanistic use of Brownian motion in ecology, highlighting that especially in rich environments, Brownian motion emerges from ecological interactions, rather than being a default movement pattern.

scholarly journals Serum Bactericidal Assays To Evaluate Typhoidal and Nontyphoidal Salmonella Vaccines

2014 ◽  
Vol 21 (5) ◽  
pp. 712-721 ◽  
Author(s):  
Mary Adetinuke Boyd ◽  
Sharon M. Tennant ◽  
Venant A. Saague ◽  
Raphael Simon ◽  
Khitam Muhsen ◽  
...  
Keyword(s):  
Sub Saharan Africa ◽  
Content Type ◽  
Vaccine Candidates ◽  
Live Attenuated Vaccines ◽  
Bactericidal Antibody ◽  
Paratyphi B ◽  
Sub Saharan ◽  
Functional Antibodies ◽  
New Vaccines

ABSTRACTInvasiveSalmonellainfections for which improved or new vaccines are being developed include enteric fever caused bySalmonella entericaserovars Typhi, Paratyphi A, and Paratyphi B and sepsis and meningitis in young children in sub-Saharan Africa caused by nontyphoidalSalmonella(NTS) serovars, particularlyS. entericaserovars Typhimurium and Enteritidis. Assays are needed to measure functional antibodies elicited by the new vaccines to assess their immunogenicities and potential protective capacities. We developedin vitroassays to quantify serum bactericidal antibody (SBA) activity induced byS. Typhi,S. Paratyphi A,S. Typhimurium, andS. Enteritidis vaccines in preclinical studies. Complement from various sources was tested in assays designed to measure antibody-dependent complement-mediated killing. Serum from rabbits 3 to 4 weeks of age provided the best complement source compared to serum from pigs, goats, horses, bovine calves, or rabbits 8 to 12 weeks of age. ForS. Enteritidis,S. Typhimurium, andS. Typhi SBA assays to be effective, bacteria had to be harvested at log phase. In contrast,S. Paratyphi A was equally susceptible to killing whether it was grown to the stationary or log phase. The typhoidal serovars were more susceptible to complement-mediated killing than were the nontyphoidal serovars. Lastly, the SBA endpoint titers correlated with serum IgG anti-lipopolysaccharide (LPS) titers in mice immunized with mucosally administeredS. Typhimurium,S. Enteritidis, andS. Paratyphi A but notS. Typhi live attenuated vaccines. The SBA assay described here is a useful tool for measuring functional antibodies elicited bySalmonellavaccine candidates.

scholarly journals Ankle Dorsiflexion Among Healthy Men With Different Qualities of Lower Extremity Movement

2014 ◽  
Vol 49 (5) ◽  
pp. 617-623 ◽  
Author(s):  
Alon Rabin ◽  
Zvi Kozol ◽  
Elad Spitzer ◽  
Aharon Finestone
Keyword(s):  
Lower Extremity ◽  
Weight Bearing ◽  
Movement Patterns ◽  
Movement Pattern ◽  
Ankle Dorsiflexion ◽  
Healthy Male ◽  
Cross Sectional ◽  
Quality Of Movement ◽  
Step Down

Context: Lower extremity movement patterns have been implicated as a risk factor for various knee disorders. Ankle-dorsiflexion (DF) range of motion (ROM) has previously been associated with a faulty movement pattern among healthy female participants. Objective: To determine the association between ankle DF ROM and the quality of lower extremity movement during the lateral step-down test among healthy male participants. Design: Cross-sectional study. Setting: Training facility of the Israel Defense Forces. Patients or Other Participants: Fifty-five healthy male Israeli military recruits (age = 19.7 ± 1.1 years, height = 175.4 ± 6.4 cm, mass = 72.0 ± 7.6 kg). Intervention(s): Dorsiflexion ROM was measured in weight-bearing and non–weight-bearing conditions using a fluid-filled inclinometer and a universal goniometer, respectively. Lower extremity movement pattern was assessed visually using the lateral step-down test and classified categorically as good or moderate. All measurements were performed bilaterally. Main Outcome Measure(s): Weight-bearing and non–weight-bearing DF ROM were more limited among participants with moderate quality of movement than in those with good quality of movement on the dominant side (P = .01 and P = .02 for weight-bearing and non–weight-bearing DF, respectively). Non–weight-bearing DF demonstrated a trend toward a decreased range among participants with moderate compared with participants with good quality of movement on the nondominant side (P = .03 [adjusted P = .025]). Weight-bearing DF was not different between participants with good and moderate movement patterns on the nondominant side (P = .10). Weight-bearing and non–weight-bearing ankle DF ROM correlated significantly with the quality of movement on both sides (P &lt; .01 and P &lt; .05 on the dominant and nondominant side, respectively). Conclusions: Ankle DF ROM was associated with quality of movement among healthy male participants. The association seemed weaker in males than in females.

scholarly journals Intranasal Vaccination with the Recombinant Listeria monocytogenes ΔactA prfA*Mutant Elicits Robust Systemic and Pulmonary Cellular Responses and Secretory Mucosal IgA

2011 ◽  
Vol 18 (4) ◽  
pp. 640-646 ◽  
Author(s):  
Jin Qiu ◽  
Lin Yan ◽  
Jianbo Chen ◽  
Crystal Y. Chen ◽  
Ling Shen ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Immune Responses ◽  
Cellular Response ◽  
Cellular Responses ◽  
Broth Culture ◽  
Intravenous Route ◽  
Content Type ◽  
Vaccine Candidates ◽  
Ifn Γ ◽  
Subcutaneous Immunization

ABSTRACTWe previously showed that recombinant (r)Listeria monocytogenescarrying ΔactAand a selectedprfA*mutation (r-ListeriaΔactA prfA*) secreted >100-fold more immunogen in broth culture than wild-type r-Listeriaor r-ListeriaΔactAand elicited much greater cellular and humoral immune responses than r-ListeriaΔactAafter intravenous vaccination of mice. Here, we conducted comparative studies evaluating vaccine-elicited immune responses in systemic and mucosal sites after intranasal, intravenous, intraperitoneal, or subcutaneous immunization of mice with r-ListeriaΔactA prfA*vaccine candidates. Intranasal vaccination of mice with r-ListeriaΔactA prfA* vaccine candidates elicited a robust gamma interferon-positive (IFN-γ+) cellular response in systemic sites, although intravenous or intraperitoneal immunization was more efficient. Surprisingly, intranasal vaccination elicited an appreciable pulmonary IFN-γ+cellular response that was nonstatistically higher than the magnitude induced by the intravenous route but was significantly greater than that elicited by subcutaneous immunization. Furthermore, although intranasal r-ListeriaΔactA prfA*delivery induced poor systemic IgG responses, intranasal vaccination elicited appreciable secretory immunogen-specific IgA titers that were similar to or higher in mucosal fluid than those induced by subcutaneous and intravenous immunizations. Thus, intranasal vaccination with r-ListeriaΔactA prfA*appears to be a useful approach for eliciting robust systemic and pulmonary cellular responses and measurable secretory mucosal IgA titers.

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