High prevalence rate of digestive tract bacteria in duodenoscopes: a nationwide study

ObjectiveIncreasing numbers of outbreaks caused by contaminated duodenoscopes used for Endoscopic Retrograde Cholangiopancreatography (ERCP) procedures have been reported, some with fatal outcomes. We conducted a nationwide cross-sectional study to determine the prevalence of bacterial contamination of reprocessed duodenoscopes in The Netherlands.DesignAll 73 Dutch ERCP centres were invited to sample ≥2 duodenoscopes using centrally distributed kits according to uniform sampling methods, explained by video instructions. Depending on duodenoscope type, four to six sites were sampled and centrally cultured. Contamination was defined as (1) any microorganism with ≥20 colony forming units (CFU)/20 mL (AM20) and (2) presence of microorganisms with gastrointestinal or oral origin, independent of CFU count (MGO).ResultsSixty-seven out of 73 centres (92%) sampled 745 sites of 155 duodenoscopes. Ten different duodenoscope types from three distinct manufacturers were sampled including 69 (46%) Olympus TJF-Q180V, 43 (29%) Olympus TJF-160VR, 11 (7%) Pentax ED34-i10T, 8 (5%) Pentax ED-3490TK and 5 (3%) Fujifilm ED-530XT8. Thirty-three (22%) duodenoscopes from 26 (39%) centres were contaminated (AM20). On 23 (15%) duodenoscopes MGO were detected, including Enterobacter cloacae, Escherichia coli, Klebsiella pneumonia and yeasts. For both definitions, contamination was not duodenoscope type dependent (p values: 0.20 and higher).ConclusionIn 39% of all Dutch ERCP centres, at least one AM20-contaminated patient-ready duodenoscope was identified. Fifteen per cent of the duodenoscopes harboured MGO, indicating residual organic material of previous patients, that is, failing of disinfection. These results suggest that the present reprocessing and process control procedures are not adequate and safe.

Download Full-text

Study of current status of bacteriological prevalence and profile in an inborn unit of SNCU in central India

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20181436 ◽

2018 ◽

Vol 5 (3) ◽

pp. 764 ◽

Cited By ~ 1

Author(s):

Avyact Agrawal ◽

Sukarn Awasthi ◽

Pawan Ghanghoriya ◽

Shivraj Singh

Keyword(s):

Central India ◽

Cross Sectional Study ◽

Current Status ◽

Klebsiella Pneumonia ◽

Sensitivity Testing ◽

Cross Sectional ◽

E Coli ◽

Medical College ◽

High Prevalence ◽

Culture Positive

Background: About 43% of the under-five child mortality is contributed by neonatal death. According to National Neonatal Perinatal Database (NNPD) in inborn births, Klebsiella pneumonia was the most commonly associated pathogen, followed by Staphylococcus aureus. Hence to know the prevalence and profile of bacterial infection in the inborn unit of an SNCU in Central India,this study was done.Methods: This is a cross sectional study done in the Inborn Unit of SNCU, Department of Paediatrics, Netaji Subhash Chandra Bose Medical College and Hospital, Jabalpur (M.P.), INDIA done between 1st March 2015 to 31st August 2016 where the blood sample and data collection of the suspected patients was done. Samples were then sent for culture and sensitivity testing. All the details then entered in Microsoft Excel Sheet and data was analysed using SPSS v 20.Results: The prevalence of bacteriologically positive sepsis was found to be 5.06% (in 43patients out of 850 examined cases). It was more prevalent among males that is in 24 among total 43 culture positives (55.8%), low birth weight were 37 (86% of culture positive patients)and preterm were 35(81.4% of culture positive patients). The most common pathogen associated was found to be Klebsiella pneumonia which was detected in 16 patients (37.2% of all bacteriologically positive patients) followed by E. coli and Pseudomonas, each in 7 patients (16.28%).Conclusions: Though we are on the track of minimising morbidities still we have a high prevalence of neonatal sepsis in inborn unit so sepsis related morbidities can be prevented if timely interventions are done.

Download Full-text

Minerals Intake Distributions in a Large Sample of Iranian at-Risk Population Using the National Cancer Institute Method: Do They Meet Their Requirements?

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000232 ◽

2015 ◽

Vol 85 (3-4) ◽

pp. 129-144 ◽

Cited By ~ 3

Author(s):

Zahra Heidari ◽

Awat Feizi ◽

Leila Azadbakht ◽

Nizal Sarrafzadegan

Keyword(s):

At Risk ◽

National Cancer Institute ◽

Population Based ◽

Cross Sectional Study ◽

Normal Function ◽

Dietary Intakes ◽

Middle Aged ◽

Cross Sectional ◽

Nutritional Interventions ◽

High Prevalence

Abstract. Background: Minerals are required for the body’s normal function. Aim: The current study assessed the intake distribution of minerals and estimated the prevalence of inadequacy and excess among a representative sample of healthy middle aged and elderly Iranian people. Methods: In this cross-sectional study, the second follow up to the Isfahan Cohort Study (ICS), 1922 generally healthy people aged 40 and older were investigated. Dietary intakes were collected using 24 hour recalls and two or more consecutive food records. Distribution of minerals intake was estimated using traditional (averaging dietary intake days) and National Cancer Institute (NCI) methods, and the results obtained from the two methods, were compared. The prevalence of minerals intake inadequacy or excess was estimated using the estimated average requirement (EAR) cut-point method, the probability approach and the tolerable upper intake levels (UL). Results: There were remarkable differences between values obtained using traditional and NCI methods, particularly in the lower and upper percentiles of the estimated intake distributions. A high prevalence of inadequacy of magnesium (50 - 100 %), calcium (21 - 93 %) and zinc (30 - 55 % for males > 50 years) was observed. Significant gender differences were found regarding inadequate intakes of calcium (21 - 76 % for males vs. 45 - 93 % for females), magnesium (92 % vs. 100 %), iron (0 vs. 15 % for age group 40 - 50 years) and zinc (29 - 55 % vs. 0 %) (all; p < 0.05). Conclusion: Severely imbalanced intakes of magnesium, calcium and zinc were observed among the middle-aged and elderly Iranian population. Nutritional interventions and population-based education to improve healthy diets among the studied population at risk are needed.

Download Full-text

HIGH PREVALENCE OF MALNUTRITON AND WORM INFECTION AMONG VAN KIEU AND PAKOH CHILDREN AGED 12-36 MONTHS IN DAKRONG DISTRICT, QUANG TRI PROVINCE

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2012.5.16 ◽

2012 ◽

pp. 129-134

Author(s):

Thi Lan Tran ◽

Thi Huong Le ◽

Xuan Ninh Nguyen

Keyword(s):

Cross Sectional Study ◽

Age Group ◽

Infection Status ◽

Sectional Study ◽

Group Recommendation ◽

Related Factors ◽

Normal Children ◽

Cross Sectional ◽

High Prevalence ◽

Very High

Objectives: Assess the nutritional status, worm infection status and some related factors among children aged 12-36 months of Dakrong district, Quang Tri province. Subject and method: A cross sectional study was carried out in 2010, in 680 children aged 12-36 months in 4 communes of Dakrong district, Quang Tri province. Results: The malnutrition rate was 55.0% for underweight, 66.5% for stunting and 16.2% for wasting. The prevalence of malnutrition increases by age group. The prevalence of worm infection was 31.6%, the highest prevalence was belong to Ascaris infection (24.6%), followed by Hookworm and Trichuris (6.5% and 6.2%, respectively). The prevalence of worm infection among children under two is very high (27.0%). The prevalence of worm infection was distributed quite equally between the malnutrition children group and normal children group. Recommendation: Early deworming forchildren from 12 months should be considered as important strategy against the malnutrition of children in Dakrong district, Quang Tri province

Download Full-text

Prevalence of MDR organism (MDRO) carriage in children and their household members in Siem Reap Province, Cambodia

JAC-Antimicrobial Resistance ◽

10.1093/jacamr/dlaa097 ◽

2020 ◽

Vol 2 (4) ◽

Author(s):

Shweta R Singh ◽

Bunsoth Mao ◽

Konstantin Evdokimov ◽

Pisey Tan ◽

Phana Leab ◽

...

Keyword(s):

Final Analysis ◽

Cross Sectional Study ◽

Cross Sectional ◽

Middle Income ◽

E Coli ◽

Middle Income Countries ◽

Siem Reap ◽

High Prevalence ◽

Significant Public Health ◽

History Of

Abstract Background The rising incidence of infections caused by MDR organisms (MDROs) poses a significant public health threat. However, little has been reported regarding community MDRO carriage in low- and middle-income countries. Methods We conducted a cross-sectional study in Siem Reap, Cambodia comparing hospital-associated households, in which an index child (age: 2–14 years) had been hospitalized for at least 48 h in the preceding 2–4 weeks, with matched community households on the same street, in which no other child had a recent history of hospitalization. Participants were interviewed using a survey questionnaire and tested for carriage of MRSA, ESBL-producing Enterobacterales (ESBL-E) and carbapenemase-producing Enterobacterales (CPE) by culture followed by antibiotic susceptibility testing. We used logistic regression analysis to analyse associations between collected variables and MDRO carriage. Results Forty-two pairs of households including 376 participants with 376 nasal swabs and 290 stool specimens were included in final analysis. MRSA was isolated from 26 specimens (6.9%). ESBL-producing Escherichia coli was detected in 269 specimens (92.8%) whereas ESBL-producing Klebsiella pneumoniae was isolated from 128 specimens (44.1%), of which 123 (42.4%) were co-colonized with ESBL-producing E. coli. Six (2.1%) specimens tested positive for CPE (4 E. coli and 2 K. pneumoniae). The prevalence ratios for MRSA, ESBL-producing E. coli and ESBL-producing K. pneumoniae carriage did not differ significantly in hospital-associated households and hospitalized children compared with their counterparts. Conclusions The high prevalence of ESBL-E across both household types suggests that MDRO reservoirs are common in the community. Ongoing genomic analyses will help to understand the epidemiology and course of MDRO spread.

Download Full-text

Prevalence of urogenital, anal, and pharyngeal infections with Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium: a cross-sectional study in Reunion island

BMC Infectious Diseases ◽

10.1186/s12879-021-05801-9 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

A. Calas ◽

N. Zemali ◽

G. Camuset ◽

J. Jaubert ◽

R. Manaquin ◽

...

Keyword(s):

Chlamydia Trachomatis ◽

Neisseria Gonorrhoeae ◽

Mycoplasma Genitalium ◽

Cross Sectional Study ◽

Sectional Study ◽

Transmitted Infection ◽

Cross Sectional ◽

Sexually Transmitted ◽

Urogenital Infection ◽

High Prevalence

Abstract Background Recommendations for sexually transmitted infection (STI) screening vary significantly across countries. This study evaluated the prevalence of urogenital and extragenital infections with Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Mycoplasma genitalium (MG) in patients visiting a French STI clinic in the Indian Ocean region to determine whether current STI screening practices should be updated. Methods This cross-sectional study examined all patients who visited the STI clinic between 2014 and 2015. Triplex polymerase chain reaction screening for CT, NG, and MG was performed on urine, vaginal, pharyngeal, and anal specimens (FTD Urethritis Basic Kit, Fast Track Diagnostics, Luxembourg). Results Of the 851 patients enrolled in the study, 367 were women (367/851, 43.2%) and 484 were men (484/851, 56.0%). Overall, 826 urogenital specimens (826/851, 97.1%), 606 pharyngeal specimens (606/851, 71.2%), and 127 anal specimens (127/851, 14.9%) were taken from enrolled patients. The prevalence of urogenital CT and MG was high in women ≤25 years (19/186, 10.21%; 5/186, 2.69%) and in men who have sex with women ≤30 years (16/212, 7.54%; 5/212, 2.36%). Among patients with urogenital CT infection, 13.7% (7/51) had urethritis. All patients with urogenital MG infection were asymptomatic. Men who have sex with men had a high prevalence of pharyngeal CT (2/45, 4.44%) and NG (3/44, 6.81%) and a high prevalence of anal CT (2/27, 7.41%), NG (2/27, 7.40%), and MG (1/27, 3.70%). After excluding patients with concomitant urogenital infection, extragenital infections with at least 1 of the 3 pathogens were found in 20 swabs (20/91, 21.9%) taken from 16 patients (16/81, 19.7%), all of them asymptomatic. Conclusions Routine multisite screening for CT, NG, and MG should be performed to mitigate the transmission of STIs in high-risk sexually active populations.

Download Full-text

High prevalence of syphilis in women, minors, and precarious patients: a cross‐sectional study in a Reunion Island sexually transmitted infection clinic, 2017‐2020

Journal of the European Academy of Dermatology and Venereology ◽

10.1111/jdv.17572 ◽

2021 ◽

Author(s):

A. Delfosse ◽

N. Bouscaren ◽

N. Dupin ◽

J. Jaubert ◽

PL. Tran ◽

...

Keyword(s):

Sexually Transmitted Infection ◽

Cross Sectional Study ◽

Sectional Study ◽

Transmitted Infection ◽

Cross Sectional ◽

Sexually Transmitted ◽

Sexually Transmitted Infection Clinic ◽

High Prevalence ◽

Réunion Island ◽

Infection Clinic

Download Full-text

Evaluation of Nutritional Risk and Prevalence of Obesity in Patients with Covid-19 in A Reference Hospital in Salvador, Bahia, Brazil: A Cross-Sectional Study

International Journal of Nutrology ◽

10.1055/s-0041-1728680 ◽

2021 ◽

Vol 14 (01) ◽

pp. 011-015

Author(s):

Rodrigo Fernandes Weyll Pimentel ◽

Gilson Cruz de Moraes ◽

Shalla Gomes Cavalcanti Barcelos ◽

Pedro Carlos Muniz de Figueiredo ◽

Magno Conceição Das Merces

Keyword(s):

Cross Sectional Study ◽

Nutritional Risk ◽

Sectional Study ◽

Cross Sectional ◽

High Prevalence ◽

Reference Hospital ◽

Prevalence Of Obesity ◽

Nrs 2002 ◽

Study Type ◽

Nutritional Risk Screening

Abstract Introduction The Coronavirus has spread to almost every country in the world, causing the coronavirus disease (COVID-19). The coronavirus stands out among the other infections especially by it's high contagious power and important effects on the respiratory system. The COVID-19 has differents ways of presentation and these are influenced by the patient's previous nutricional status, correlated with the patient's lifestyle and comorbities. Objective this survey seeks to analyze the nutritional status and the prevalence of obesity in patients hospitalized with SARS-CoV-2. Methods: this is a descriptive, prospective and cross-sectional study type, which 41 patients affected by COVID-19 were interviewed. Patient's weight and height were used to assess the BMI, and nutritional risk assessment was performed using the Nutritional Risk Screening tool (NRS 2002). For the analysis, Absolute (AF) and Relative Frequency (RF), the mean and the standard deviation were calculated. Results It was observerd that 78% of the participants had a high nutritional risk, while only 22% had a low nutritional risk. Besides that, 34% showed overweight and 41.4% showed obesity. Conclusion the existence of a high prevalence of increased nutritional risk was evidenced, in addition to the high frequency of overweight in patients affected by SARS-CoV-2.

Download Full-text

Revisiting Konzo Risk Factors in Three Areas Differently Affected by Spastic Paraparesis in Eastern Democratic Republic of the Congo Discloses a Prominent Role of the Nutritional Status—A Comparative Cross-Sectional Study

Nutrients ◽

10.3390/nu13082628 ◽

2021 ◽

Vol 13 (8) ◽

pp. 2628

Author(s):

Marius Baguma ◽

Espoir Bwenge Malembaka ◽

Esto Bahizire ◽

Germain Zabaday Mudumbi ◽

Dieudonné Bahati Shamamba ◽

...

Keyword(s):

Risk Factors ◽

Nutritional Status ◽

Democratic Republic ◽

Cross Sectional Study ◽

Sectional Study ◽

Cyanide Poisoning ◽

Cross Sectional ◽

High Prevalence ◽

Republic Of The Congo

This comparative cross-sectional study aimed to better understand the respective contributions of protein malnutrition and cassava-derived cyanide poisoning in the development of konzo. We compared data on nutritional status and cyanide exposure of school-age adolescent konzo-diseased patients to those of non-konzo subjects of similar age from three areas in the Eastern Democratic Republic of the Congo. Our results show that konzo patients had a high prevalence of both wasting (54.5%) and stunting (72.7%), as well as of cyanide poisoning (81.8%). Controls from Burhinyi and those from Idjwi showed a similar profile with a low prevalence of wasting (3.3% and 6.5%, respectively) and intermediate prevalence of stunting (26.7% and 23.9%, respectively). They both had a high prevalence of cyanide poisoning (50.0% and 63.0%, respectively), similar to konzo-patients. On the other hand, controls from Bukavu showed the lowest prevalence of both risk factors, namely chronic malnutrition (12.1%) and cyanide poisoning (27.6%). In conclusion, cassava-derived cyanide poisoning does not necessarily coexist with konzo outbreaks. The only factor differentiating konzo patients from healthy individuals exposed to cyanide poisoning appeared to be their worse nutritional status. This further suggests that, besides the known role of cyanide poisoning in the pathogenesis of konzo, malnutrition may be a key factor for the disease occurrence.

Download Full-text

Waist circumference and waist circumference to height ratios of Kaingáng indigenous adolescents from the State of Rio Grande do Sul, Brazil

Cadernos de Saúde Pública ◽

10.1590/s0102-311x2012001100005 ◽

2012 ◽

Vol 28 (11) ◽

pp. 2053-2062 ◽

Cited By ~ 4

Author(s):

Teresa Gontijo de Castro ◽

Laura A. Barufaldi ◽

Michael Maia Schlüssel ◽

Wolney Lisboa Conde ◽

Maurício Soares Leite ◽

...

Keyword(s):

Waist Circumference ◽

Correlation Coefficients ◽

Cross Sectional Study ◽

Rio Grande Do Sul ◽

Sectional Study ◽

Cross Sectional ◽

School Based ◽

High Prevalence ◽

Z Scores ◽

Indigenous Adolescents

The aim of this study was to describe the distribution of waist circumference (WC) and WC to height (WCTH) values among Kaingáng indigenous adolescents in order to estimate the prevalence of high WCTH values and evaluate the correlation between WC and WCTH and body mass index (BMI)-for-age. A total of 1,803 indigenous adolescents were evaluated using a school-based cross-sectional study. WCTH values > 0.5 were considered high. Higher mean WC and WCTH values were observed for girls in all age categories. WCTH values > 0.5 were observed in 25.68% of the overall sample of adolescents. Mean WC and WCTH values were significantly higher for adolescents with BMI/age z-scores > 2 than for those with normal z-scores. The correlation coefficients of WC and WCTH for BMI/age were r = 0.68 and 0.76, respectively, for boys, and r = 0.79 and 0.80, respectively, for girls. This study highlights elevated mean WC and WCTH values and high prevalence of abdominal obesity among Kaingáng indigenous adolescents.

Download Full-text