IDDF2021-ABS-0207 Type 2 resistant starch improves liver steatosis induced by high-fat diet relating to gut microbiota regulation and concentration of propionic acid in portal vein blood in C57BL/6J mice

Here, we investigated the roles and mechanisms of flavonoids from mulberry leaves (FML) on lipid metabolism in high fat diet (HFD)-fed mice. ICR mice were fed either a control diet (Con) or HFD with or without FML (240 mg/kg/day) by oral gavage for six weeks. FML administration improved lipid accumulation, alleviated liver steatosis and the whitening of brown adipose tissue, and improved gut microbiota composition in HFD-fed mice. Microbiota transplantation from FML-treated mice alleviated HFD-induced lipid metabolic disorders. Moreover, FML administration restored the production of acetic acid in HFD-fed mice. Correlation analysis identified a significant correlation between the relative abundances of Bacteroidetes and the production of acetic acid, and between the production of acetic acid and the weight of selected adipose tissues. Overall, our results demonstrated that in HFD-fed mice, the lipid metabolism improvement induced by FML administration might be mediated by gut microbiota, especially Bacteroidetes-triggered acetic acid production.

Download Full-text

Black rice anthocyanins alleviate hyperlipidemia, liver steatosis and insulin resistance by regulating lipid metabolism and gut microbiota in obese mice

Food & Function ◽

10.1039/d1fo01394g ◽

2021 ◽

Author(s):

Haizhao Song ◽

Xinchun Shen ◽

Yang Zhou ◽

Xiaodong Zheng

Keyword(s):

Insulin Resistance ◽

Lipid Metabolism ◽

Gut Microbiota ◽

Hepatic Steatosis ◽

High Fat Diet ◽

Liver Steatosis ◽

Obese Mice ◽

Black Rice ◽

High Fat ◽

Diet Induced Obesity

Supplementation of black rice anthocyanins (BRAN) alleviated high fat diet-induced obesity, insulin resistance and hepatic steatosis by improvement of lipid metabolism and modification of the gut microbiota.

Download Full-text

Lactobacillus acidophilus alleviates type 2 diabetes by regulating hepatic glucose, lipid metabolism and gut microbiota in mice

Food & Function ◽

10.1039/c9fo01062a ◽

2019 ◽

Vol 10 (9) ◽

pp. 5804-5815 ◽

Cited By ~ 17

Author(s):

Fenfen Yan ◽

Na Li ◽

Jialu Shi ◽

Huizhen Li ◽

Yingxue Yue ◽

...

Keyword(s):

Type 2 Diabetes ◽

Lipid Metabolism ◽

Gut Microbiota ◽

Lactobacillus Acidophilus ◽

High Fat Diet ◽

High Fat ◽

Glucose And Lipid Metabolism ◽

Hepatic Glucose

Lactobacillus acidophilus alleviates type 2 diabetes induced by a high fat diet and streptozotocin (STZ) injection by regulating gut microbiota, hepatic glucose and lipid metabolism in mice.

Download Full-text

Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice

Nutrients ◽

10.3390/nu11030670 ◽

2019 ◽

Vol 11 (3) ◽

pp. 670 ◽

Cited By ~ 20

Author(s):

Lihua Han ◽

Tiange Li ◽

Min Du ◽

Rui Chang ◽

Biyuan Zhan ◽

...

Keyword(s):

Gut Microbiota ◽

Inflammatory Cytokines ◽

High Fat Diet ◽

Intestinal Inflammation ◽

Water Extract ◽

Perennial Herb ◽

Diabetic Mice ◽

High Fat ◽

Type 2 Diabetic

Potentilla discolor Bunge (PDB), a perennial herb, has been used as a traditional Chinese medicine in the therapy of many diseases. The aim of the current study was to investigate the effect of PDB water extract on systemic inflammation and gut microbiota in type 2 diabetic (T2D) mice induced by high-fat diet (HFD) and streptozotocin (STZ) injection. C57BL/6J mice were randomly divided into a normal diet (ND) group, T2D group, and PDB group (diabetic mice treated with PDB water extract at a dose of 400 mg/kg body weight). Results showed that PDB significantly decreased the levels of lipopolysaccharide (LPS) and pro-inflammatory cytokines in serum. Further investigation showed that PDB significantly reduced the ratio of Firmicutes/Bacteroidetes and the relative abundance of Proteobacteria in fecal samples of diabetic mice. In addition, PDB notably alleviated intestinal inflammation as evidenced by decreased expression of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor-κB (NF-κB), and inflammatory cytokines. PDB also reversed the decreased expression of intestinal mucosal tight junction proteins including Claudin3, ZO-1, and Occludin. Meanwhile, the levels of fecal acetic acid and butyric acid and their specific receptors including G-protein-coupled receptor (GPR) 41 and 43 expression in the colon were also increased after PDB treatment. Our results indicated that PDB might serve as a potential functional ingredient against diabetes and related inflammation.

Download Full-text

Polysaccharide from tuberous roots of Ophiopogon japonicus regulates gut microbiota and its metabolites during alleviation of high-fat diet-induced type-2 diabetes in mice

Journal of Functional Foods ◽

10.1016/j.jff.2019.103593 ◽

2019 ◽

Vol 63 ◽

pp. 103593 ◽

Cited By ~ 3

Author(s):

Huai-You Wang ◽

Lin-Xiu Guo ◽

Wei-Hui Hu ◽

Zhi-Tian Peng ◽

Cheng Wang ◽

...

Keyword(s):

Type 2 Diabetes ◽

Gut Microbiota ◽

High Fat Diet ◽

High Fat ◽

Diabetes In Mice ◽

Tuberous Roots ◽

Ophiopogon Japonicus

Download Full-text

SCGN Administration prevents Insulin Resistance and Diabetic Complications in High-Fat Diet Fed Animals

10.1101/189324 ◽

2017 ◽

Cited By ~ 1

Author(s):

Anand Kumar Sharma ◽

Radhika Khandelwal ◽

Swathi Chadalawada ◽

N Sai Ram ◽

T Avinash Raj ◽

...

Keyword(s):

High Fat Diet ◽

Insulin Action ◽

Therapeutic Potential ◽

Liver Steatosis ◽

Insulin Response ◽

Cell Regeneration ◽

High Fat ◽

Β Cell ◽

Associated Risk Factors

AbstractSecretagogin (SCGN) is poorly-studied secretory/cytosolic CaBP enriched in pancreatic β-cells. Recent studies implicated SCGN in diabetes; however, its function and therapeutic prospect remain uncharted. Based on the apparent synchrony of SCGN and insulin secretion (and its disruption in HFD-fed animals) and considering SCGN downregulation in Type 2 diabetes, we hypothesized that SCGN is a key regulator of insulin response. To test this, we administered rSCGN to HFD-fed animals. We here report that a novel SCGN-insulin interaction stabilizes insulin and potentiates insulin action. Moreover, a chronic rSCGN administration improves insulin response and alleviates obesity associated risk factors such as weight gain, liver steatosis and cholesterol imbalance in DIO animals. Beside the anti-diabetic effects, prolonged rSCGN treatment also induces β-cell regeneration. These effects seem to originate from SCGN mediated regulation of insulin concentration & function as validated in insulin-deficient STZ animals. Our results demonstrate the prospects of the therapeutic potential of SCGN against diabetes.

Download Full-text

The Regulation Effect of Crude Polysaccharides from Cordyceps Militaris on Obesity Control and Gut Microbiota Community via High-Fat Diet-Fed Mice Model

10.20944/preprints202012.0052.v1 ◽

2020 ◽

Author(s):

Minglei Yu ◽

Nan Hui ◽

Kashif Hayat ◽

Xijia Yang ◽

Shaohua Chu ◽

...

Keyword(s):

Gut Microbiota ◽

High Fat Diet ◽

Liver Steatosis ◽

Serum Triglyceride ◽

Cordyceps Militaris ◽

High Yield ◽

Serum Triglyceride Level ◽

Mice Model ◽

High Fat ◽

Brown Adipose

Administration of crude polysaccharides extract from natural product is a promising gut microbiota-targeted approach to preventing obesity and associated metabolic disorders. Dietary restrictions can change the type and number of gut bacteria, which is an important factor in delaying the onset and burden of diseases. This study aimed to investigate the effects of high-yield crude polysaccharides from Cordyceps militaris (CMP) on high-fat diet (HFD) mice model and the gut microbiota community assembly, and to identify whether selenium (Se) addition would improve CMP action mode during cultivation. We found that the CMP treatment ameliorated adipose and liver pathologic morphology and fat accumulation in obese mice, while, SeCMP intervention was not superior than CMP in body mass gain, but notably decreasing serum triglyceride level increased by HFD. The upregulated expression of gene Cyp7a1 in liver and protein UCP1 in brown adipose tissue (BAT) preliminary indicated that the effect might relate to bile acids (BAs) metabolism pathway and thermogenesis. In addition, CMP showed a drastic decrease in the gut microbes which positively correlated with dyslipidemia parameters. Our result reveals the potential of CMP to be used as functional food in the prevention of diet-induced adipose and liver steatosis, so does SeCMP has outstanding capacity of improving dyslipidemia.

Download Full-text

Obesity-Related Metabolome and Gut Microbiota Profiles of Juvenile Göttingen Minipigs—Long-Term Intake of Fructose and Resistant Starch

Metabolites ◽

10.3390/metabo10110456 ◽

2020 ◽

Vol 10 (11) ◽

pp. 456

Author(s):

Mihai V. Curtasu ◽

Valeria Tafintseva ◽

Zachary A. Bendiks ◽

Maria L. Marco ◽

Achim Kohler ◽

...

Keyword(s):

Fatty Acids ◽

Gut Microbiota ◽

Resistant Starch ◽

High Fat Diet ◽

Short Chain Fatty Acids ◽

Short Chain ◽

High Fat ◽

Obesity And Diabetes ◽

Ad Libitum ◽

Chain Fatty Acids

The metabolome and gut microbiota were investigated in a juvenile Göttingen minipig model. This study aimed to explore the metabolic effects of two carbohydrate sources with different degrees of risk in obesity development when associated with a high fat intake. A high-risk (HR) high-fat diet containing 20% fructose was compared to a control lower-risk (LR) high-fat diet where a similar amount of carbohydrate was provided as a mix of digestible and resistant starch from high amylose maize. Both diets were fed ad libitum. Non-targeted metabolomics was used to explore plasma, urine, and feces samples over five months. Plasma and fecal short-chain fatty acids were targeted and quantified. Fecal microbiota was analyzed using genomic sequencing. Data analysis was performed using sparse multi-block partial least squares regression. The LR diet increased concentrations of fecal and plasma total short-chain fatty acids, primarily acetate, and there was a higher relative abundance of microbiota associated with acetate production such as Bacteroidetes and Ruminococcus. A higher proportion of Firmicutes was measured with the HR diet, together with a lower alpha diversity compared to the LR diet. Irrespective of diet, the ad libitum exposure to the high-energy diets was accompanied by well-known biomarkers associated with obesity and diabetes, particularly branched-chain amino acids, keto acids, and other catabolism metabolites.

Download Full-text