Solute leakage from susceptible and tolerant cultivars of Phaseolus vulgaris following ozone exposure

The leakage of total electrolytes, potassium, calcium, sugars, and amino acids from primary leaf discs of Phaseolus vulgaris L. cv. Seafarer (ozone susceptible) and cv. Goldcrop (ozone tolerant) was monitored following exposure to 40 parts per hundred million (pphm) ozone for 4 h. Significant differences in cultivar response were not detected until 24 h after exposure. At that time, leakage from cv. Seafarer leaves was 220% for total electrolytes, 230% for calcium, 260% for potassium and sugars, and 400% for amino acids relative to controls, whereas the leakage from cv. Goldcrop leaves remained at control levels. The water-soluble sugar and free amino acid content of a leaf homogenate also increased at the 24-h sampling time. These accumulations may result from a reduced rate of phloem translocation. Rates of leakage and leaf-solute contents returned to control levels by 48 and 72 h after exposure.Kinetic analysis of solute efflux from leaf discs sampled from both cultivars immediately after ozone exposure indicated that these leaves contained an increased quantity of solutes which could be rapidly leached within 0.5 h. However, the subsequent linear rate of leakage between 0.5 and 4 h did not increase except in leaf discs sampled from cv. Seafarer 24 h after exposure. The results are discussed in relation to the mechanism of ozone injury in cv. Seafarer and the basis of ozone tolerance in the cv. Goldcrop.

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Effects of Different Light Intensity on the Growth and Nutrients Content of Gynura

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.955-959.3766 ◽

2014 ◽

Vol 955-959 ◽

pp. 3766-3769 ◽

Cited By ~ 2

Author(s):

Xiang Rong Cheng ◽

Jia Liu ◽

Jun Shu ◽

Mu Kui Yu

Keyword(s):

Light Intensity ◽

Sugar Content ◽

Soluble Sugar ◽

Light Condition ◽

Amino Acid Content ◽

Canopy Cover ◽

Water Soluble ◽

Light Conditions ◽

Significant Difference ◽

Number Of Branches

Light condition has significant effects on plant growth and nutrient accumulation. The growth and nutrients content of Gynura (Begonia fimbristipulata) were studied under three light intensities (natural field, 100% full sunlight (L0); 50% canopy cover (L1); 70% canopy cover (L2)) in Fuyang, China. The results showed that plant height, ground diameter and number of branches were no significant difference between L0 and L1 treatments (P>0.05), these variables were significantly lower in L2 treatment than that in L0 treatment (P<0.05). Potassium (K), phosphorus (P), vitamin C (Vc) and soluble sugar content in Gynura’s leaves reduced with decreasing light intensity. The content of protein and iron (Fe) were no significant difference among the three light treatments (P>0.05). The content of calcium (Ca) markedly increased in L1 treatment compared with L0 treatment (P<0.05), and it greatly declined in L2 treatment. The change of light conditions had less impact on the water-soluble amino acid content (except for alanine, serine, and glutamic acid). These results indicate that Gynura is suitable to cultivate under the forest with 50% canopy cover.

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Variation in protein and amino acids content among landraces of common bean (Phaseolus vulgaris L.)

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2020.v32.i10.2175 ◽

2020 ◽

pp. 750

Author(s):

Ángel Ramón Flores-Sosa ◽

Elia Nora Aquino-Bolaños ◽

Anaberta Cardador-Martínez ◽

José Luis Chávez-Servia ◽

Araceli Minerva Vera-Guzmán ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Phaseolus Vulgaris ◽

Acid Content ◽

High Performance ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Amino Acid Profile ◽

Phaseolus Vulgaris L ◽

Genetic Characteristics

Native bean populations (Phaseolus vulgaris L.) provide bioactive and nutrient compounds; however, their amino acid profiles are unknown. Therefore, the aim of this study is to evaluate the protein content and amino acid profile of 46 native bean populations cultivated by small farmers in Oaxaca, Mexico, and compare them with that of commercial beans. Through high-performance liquid chromatography (HPLC), 16 amino acids were identified and quantified in all samples. The region of origin influenced the concentrations of amino acids. The Santa Lucia Miahuatlan populations stood out for their high content of isoleucine, threonine, methionine, arginine, serine, alanine, tyrosine, and cysteine. Amino acid content showed high variability among the populations; accessions labeled as FSLM22, FSLM27, FSLM28, and FSLM32 were enriched in aliphatic, hydroxylated, aromatic, acidic, and basic amino acids, while the FSLM14, FSLM17, and FSLM18 populations had the highest concentrations of sulfur amino acids. The FSLM01, FSLM22, FSLM27, FSLM28, FSLM30, and FSLM32 populations frequently displayed the highest concentrations of essential amino acids. The findings show that samples of native populations are highly variable in amino acid content due to the genetic characteristics of cultivated beans, environmental and agroecological influences, and crop management by farmers. The beans populations stood out can be used for direct use or a basis for the initiation of a breeding program.

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Impacts of Nitrogen (Urea) Fertilization on Cadmium Accumulation Nitrate Contents and Nutritive Values in Chinese Cabbage

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.393-395.809 ◽

2011 ◽

Vol 393-395 ◽

pp. 809-817

Author(s):

Li Zhao ◽

Fei Juan Wang ◽

Hui Yang ◽

Yu Xing Duan ◽

Chen Zhu

Keyword(s):

Amino Acids ◽

Food Security ◽

Chinese Cabbage ◽

Nitrogen Fertilization ◽

Soluble Sugar ◽

Water Soluble ◽

Natural Soil ◽

Edible Part ◽

Nutritive Values ◽

Urea Fertilization

Pot-mode cultivation experiments were carried out to study the impacts of nitrogen（urea）fertilization in different level on Cadmium (Cd) accumulation, nitrate contents and nutritive values in Chinese cabbage, The results showed that: at different level of Cd artificial enriched soil, with applied urea increased, the accumulation of Cd in edible part of Chinese cabbage gradually decreased. Nitrate Contents in edible part have a positive correlation with the concentration of Cd in soil and the nitrogen fertilizer applied. In the natural soil (with contaminated Cd in concentration of 0.18 mg/kg), when the urea was fertilized by 255 kg/hm2 to 330 kg/hm2, Cd and nitrate contents of edible parts of cabbage were within the national food security limits, which could guarantee the food security of Chinese cabbage. Increasing nitrogen fertilization in the form of urea has a certain effect on amino acids contents but little effect on soluble sugar contents in Chinese cabbage, when the concentration of urea was 320 kg/hm2, amino acids contents of cabbage reached the highest level of 355 mg/kg1. Increasing nitrogen fertilization could lower down the contents of water-soluble Cd and the available Cd in soil, thereby reduce the absorption of Cd by Chinese cabbage. To a certain extent, increasing nitrogen fertilization could increase the food security of Chinese cabbage.

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Screening of amino acid constituents from date palm fruits

International Journal of Bioassays ◽

10.21746/ijbio.2016.10.0011 ◽

2016 ◽

Vol 5 (10) ◽

pp. 4972

Author(s):

Lata Birlangi

Keyword(s):

Amino Acids ◽

Amino Acid ◽

United Arab Emirates ◽

Nutritional Value ◽

Date Palm ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Cultivated Plants ◽

Palm Fruits ◽

Social Part

The date palm (Phoenix dactylifera L.) is one of mankind’s oldest cultivated plants. The fruit of the date palm is an important crop of the hot arid and semi-arid regions of the world. It has always played a genuine economic and social part in the lives of the people of these areas. The present objective in examining the amino acid content of different varieties of date palm fruits from Middle-East region; is to determine whether its protein could effectively supplement the nutritional value and it is also aimed in finding which variety is rich in number of amino acids. The phytochemical screening revealed the presence of eight essential amino acids and five non-essential amino acids in the date fruits. Among all the date fruit varieties taken as samples for the study, Dabbas cultivar of United Arab Emirates found to exhibit eight types of amino acids which includes five as non-essential ones. Total of thirteen amino acids were detected in the seven date cultivars. Determination of amino acid can serve as a guide to the possible nutritional value.

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The effect of free amino acids and related compounds on the activity of plant enzymes. I. Effect of DL-threonine, L-proline, γ-aminobutyric acid, L-asparagine and L-cysteine on the activity of tryptophan synthase (4.2.1.20) in living bean leaf (Phaseolus vulgaris L.)

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19713323 ◽

1971 ◽

Vol 36 (9) ◽

pp. 3323-3331

Author(s):

M. Štefl ◽

I. Trčka

Keyword(s):

Amino Acids ◽

Phaseolus Vulgaris ◽

Free Amino Acids ◽

Free Amino ◽

Aminobutyric Acid ◽

Tryptophan Synthase ◽

Phaseolus Vulgaris L ◽

Plant Enzymes ◽

Related Compounds

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Putative imbalanced amino acid metabolism in rainbow trout long term fed a plant-based diet as revealed by 1H-NMR metabolomics

Journal of Nutritional Science ◽

10.1017/jns.2021.3 ◽

2021 ◽

Vol 10 ◽

Author(s):

Catherine Deborde ◽

Blandine Madji Hounoum ◽

Annick Moing ◽

Mickaël Maucourt ◽

Daniel Jacob ◽

...

Keyword(s):

Amino Acids ◽

Rainbow Trout ◽

Amino Acid ◽

Amino Acid Content ◽

Proton Nuclear Magnetic Resonance ◽

Free Form ◽

Metabolomic Analysis ◽

Nmr Metabolomics ◽

H Nmr

Abstract The long-term effect of a plant (P)-based diet was assessed by proton nuclear magnetic resonance (1H-NMR) metabolomics in rainbow trout fed a marine fish meal (FM)–fish oil (FO) diet (M), a P-based diet and a control commercial-like diet (C) starting with the first feeding. Growth performances were not heavily altered by long-term feeding on the P-based diet. An 1H-NMR metabolomic analysis of the feed revealed significantly different soluble chemical compound profiles between the diets. A set of soluble chemical compounds was found to be specific either to the P-based diet or to the M diet. Pterin, a biomarker of plant feedstuffs, was identified both in the P-based diet and in the plasma of fish fed the P-based diet. 1H-NMR metabolomic analysis on fish plasma and liver and muscle tissues at 6 and 48 h post feeding revealed significantly different profiles between the P-based diet and the M diet, while the C diet showed intermediate results. A higher amino acid content was found in the plasma of fish fed the P-based diet compared with the M diet after 48 h, suggesting either a delayed delivery of the amino acids or a lower amino acid utilisation in the P-based diet. This was associated with an accumulation of essential amino acids and the depletion of glutamine in the muscle, together with an accumulation of choline in the liver. Combined with an anticipated absorption of methionine and lysine supplemented in free form, the present results suggest an imbalanced essential amino acid supply for protein metabolism in the muscle and for specific functions of the liver.

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Interactions of water-soluble zinc porphyrin with amino acids

Inorganica Chimica Acta ◽

10.1016/s0020-1693(00)88867-2 ◽

1988 ◽

Vol 153 (4) ◽

pp. 199-200 ◽

Cited By ~ 9

Author(s):

E. Mikros ◽

A. Gaudemer ◽

R. Pasternack

Keyword(s):

Amino Acids ◽

Water Soluble ◽

Zinc Porphyrin ◽

Soluble Zinc

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Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

AMB Express ◽

10.1186/s13568-021-01217-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ramona Wördemann ◽

Lars Wiefel ◽

Volker F. Wendisch ◽

Alexander Steinbüchel

Keyword(s):

Amino Acids ◽

Dry Weight ◽

Growth Conditions ◽

Amino Acid Biosynthesis ◽

Water Soluble ◽

Lysine Content ◽

Polyaspartic Acid ◽

Acid Biosynthesis ◽

Cyanophycin Synthetase ◽

Potential Precursor

AbstractCyanophycin (multi-l-arginyl-poly-l-aspartic acid; also known as cyanophycin grana peptide [CGP]) is a biopolymer that could be used in various fields, for example, as a potential precursor for the synthesis of polyaspartic acid or for the production of CGP-derived dipeptides. To extend the applications of this polymer, it is therefore of interest to synthesize CGP with different compositions. A recent re-evaluation of the CGP synthesis in C. glutamicum has shown that C. glutamicum is a potentially interesting microorganism for CGP synthesis with a high content of alternative amino acids. This study shows that the amount of alternative amino acids can be increased by using mutants of C. glutamicum with altered amino acid biosynthesis. With the DM1729 mutant, the lysine content in the polymer could be increased up to 33.5 mol%. Furthermore, an ornithine content of up to 12.6 mol% was achieved with ORN2(Pgdh4). How much water-soluble or insoluble CGP is synthesized is strongly related to the used cyanophycin synthetase. CphADh synthesizes soluble CGP exclusively. However, soluble CGP could also be isolated from cells expressing CphA6308Δ1 or CphA6308Δ1_C595S in addition to insoluble CGP in all examined strains. The point mutation in CphA6308Δ1_C595S partially resulted in a higher lysine content. In addition, the CGP content could be increased to 36% of the cell dry weight under optimizing growth conditions in C. glutamicum ATCC13032. All known alternative major amino acids for CGP synthesis (lysine, ornithine, citrulline, and glutamic acid) could be incorporated into CGP in C. glutamicum.

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A Water-Soluble Copper-Immobilized Covalent Organic Framework Functioning as an “OFF–ON” Fluorescent Sensor for Amino Acids

Materials Advances ◽

10.1039/d1ma00234a ◽

2021 ◽

Author(s):

Lamiaa Reda Ahmed ◽

Ahmed F. M. EL-Mahdy ◽

Cheng-Tang Pan ◽

Shiao-Wei Kuo

Keyword(s):

Amino Acids ◽

Fluorescent Sensor ◽

Water Soluble ◽

One Pot ◽

Covalent Organic Framework ◽

The One ◽

Organic Framework

In this paper, we describe the construction of a new fluorescent hydroxyl- and hydrazone-based covalent organic framework (TFPB-DHTH COF) through the one-pot polycondensation of 1,3,5-tris(4-formylphenyl)benzene (TFPB) and 2,5-dihydroxyterephthalohydrazide (DHTH) under...

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