Rôle des facteurs de croissance dans la formation des structures de réserve de Verticillium dahliae et V. nigrescens

1970 ◽  
Vol 16 (9) ◽  
pp. 901-904
Author(s):  
Gilles Pelletier ◽  
Claude Aubé
Keyword(s):  
Growth Medium ◽  
Verticillium Dahliae ◽  
Culture Medium ◽  
Species Determination ◽  
Medium Temperature ◽  
Influence Of Temperature ◽  
Elapsed Time ◽  
Facteurs De Croissance

The influence of temperature, culture medium, and the elapsed time of incubation on the morphology and development of resting structures of Verticillium dahliae and V. nigrescens was determined. The development of resting structures is mainly governed by the growth medium. Temperature might be useful in species determination but the kind of medium and the age of culture are critical in this evaluation.The results are discussed more particularly in relation to the evolution of Verticillium species.

Conidial size and contents in Verticillium as affected by environmental factors

1970 ◽  
Vol 16 (4) ◽  
pp. 231-236 ◽  
Author(s):  
Gilles Pelletier ◽  
Claude Aubé
Keyword(s):  
Environmental Factors ◽  
Culture Medium ◽  
Culture Media ◽  
Species Determination ◽  
Influence Of Temperature ◽  
Spore Size ◽  
Verticillium Albo Atrum

The influence of temperature, culture medium, and age of colony on the morphology of conidia of Verticillium albo-atrum, V. dahliae, and V. nigrescens was determined. Staining solutions were used to follow the development of lipids, cytoplasm, and vacuole in conidia. The spore sizes of the Verticillium isolates differed in various culture media, temperature, and periods of growth. Spore contents could not be used to differentiate species. Spore size, therefore, is not justifiable as the only criterion for species determination in Verticillium.

MITOCHONDRIAL ACTIVITY OF NORMAL AND CANCER CELLS IN VITRO AT DIFFERENT CONCENTRATION OF DEUTERIUM IN CULTURE MEDIUM

2020 ◽  
pp. 139-141
Author(s):  
N. Antipova ◽  
A. Zlatska ◽  
R. Vasyliev ◽  
D. Zubov ◽  
S. Novikova ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Growth Medium ◽  
Culture Medium ◽  
Mitochondrial Activity

In the present study, we showed that normal and cancer cells in vitro in a deuterated growth medium show a decrease of mitochondrial activity (MA), while in a deuterium-depleted medium an increase. The publication has been prepared with the support of the "RUDN University Program 5-100".

scholarly journals Praline metabolism by germinating Lilium longiflorum pollen. I. Labelling of cytoplasmic, wall and culture medium molecules

2014 ◽  
Vol 50 (1-2) ◽  
pp. 51-66 ◽  
Author(s):  
W. V. Dashek ◽  
R. R. Mills
Keyword(s):  
Growth Medium ◽  
Culture Medium ◽  
Gel Filtration ◽  
Lilium Longiflorum ◽  
Paper Electrophoresis ◽  
Void Volume ◽  
Molecular Weights ◽  
The Common ◽  
Two Peaks ◽  
Salt Extract

Radioactivity occurs in trithloroacetic acid (TCA)-soluble and precipitable, cytoplasm and salt-washed walls following germination of <em>Lilium longiflorum</em>, cv. 'Ace' pollen in medium containing [<sup>14</sup>C]-proline (Pro). Sephadex gel filtration on G-25 through G-100 was employed to determine whether radioactivity in cytoplasm, wall and growth medium from pollen fed [<sup>14</sup>C]-Pro or [<sup>3</sup>H]=Pro plus [<sup>14</sup>C]-arafbinose (Ara) was contained within molecules possessing molecular weights of 5,000 to 100,000 daltones or greater. G-25 elution profiles of a crude cytoplasmic fraction (15,000 X g supernatant) from [<sup>14</sup>C]-Pro labelled pollen yielded a radioctive void volume peak and a retarded peak. The void volume peak contained hydroxyproline (Hyp), and exhibited a coincidence of [<sup>3</sup>H]-Pro and [<sup>14</sup>C] -Ara labelling when pollen was double labelled with the two isotopes. This peak also contained radioactivity when pollen was germinated in 2-[<sup>3</sup>H]-myo-inositol. Germination in medium supplemented with 100 µM 2,2'-dipyridyl eliminated radioactivity from 2-[<sup>3</sup>H]-myo-inositol or [<sup>14</sup>C]-,Pro in the peak. Filtratian on G-25 of aTCA-soluble fraction of a salt-extract of walls from [<sup>14</sup>C]-Pro labelled pollen resulted in void volume and two retarded peaks. Void volume and two retarded peaks were also obtained upon G-25 filtration of a cellulase-digest of walls from [M]-Pro labeled pollen. The void volume peak contained Hyp, Lys, Gly, Ala, Ser, Glu and Asp acids, Val, Tyr, Leu or lieu and Pro. Sephadex G-90, 75, and 100 elution profiles of cellulasedigests of walls from [<sup>3</sup>H]-,Pro and [<sup>14</sup>C]-Ara labelled pollen yielded radioactive retarded and Hyp-containing void volume peaks with a coincidence of [<sup>3</sup>H] and [<sup>14</sup>C] labelling. Label in the void volume was obtained when either rhozyme P11- or pepsin-digests of walls from [<sup>14</sup>C]-Pro labelled pollen were gel filtered on G-50. Paper electrophoresis coupled with paper chromatography of acid hydrolyzates of salt-washed wall fractions demonstrated 15 of the common amino acids. Gel filtration on G-25 of growth medium in which pollen was germinated resulted in two peaks, one of which eluted in the void volume. contained Hyp and excluded during subsequent gel filtration on G-100.

scholarly journals Analysis of cis-Acting Elements Required for bfpA Expression in EnteropathogenicEscherichia coli

1998 ◽  
Vol 180 (11) ◽  
pp. 3013-3016 ◽  
Author(s):  
Victor H. Bustamante ◽  
Edmundo Calva ◽  
Jose Luis Puente
Keyword(s):  
Escherichia Coli ◽  
Growth Medium ◽  
Regulatory Region ◽  
Random Mutagenesis ◽  
Ammonium Concentration ◽  
Upstream Regulatory Region ◽  
Transcriptional Activators ◽  
Promoter Elements ◽  
Medium Temperature ◽  
Cis Acting

ABSTRACT bfpA expression in enteropathogenic Escherichia coli is regulated by growth medium, temperature, and ammonium concentration and requires the BfpT protein (also called PerA), a member of the AraC family of transcriptional activators. Site-directed and PCR random mutagenesis, as well as deletion analysis of thebfpA upstream regulatory region, supported assignment of the promoter elements and demonstrated that the cis-acting elements that mediate BfpT-dependent regulation of bfpA are located between positions −85 and −46. Interestingly, this region shares 73% identity with a 40-bp-long AT-rich tract located upstream of the bfpT gene, which is essential for bfpTautoregulation.

Analysis of Heat Transfer From a Photobioreactor

2016 ◽  
Author(s):  
Gary A. Anderson ◽  
Sarmila Katuwal ◽  
Anil Kommareddy ◽  
Stephen Gent
Keyword(s):  
Relative Humidity ◽  
Ambient Temperature ◽  
Heat Loss ◽  
Growth Medium ◽  
Flow Rate ◽  
Air Flow ◽  
Air Flow Rate ◽  
Medium Temperature ◽  
Reactor Volume ◽  
Light Passing

A photobioreactor (PBR) was operated for sixteen days producing S. Leopoliensis. The PBR was lit by two LED panels, one on each of the long sides of the PBR. The PBR dimensions were nominally 51mm by 273mm with a height of 319mm (273mm liquid depth). Each LED panel was powered at 14.1W (11.2V and 1.26A). Measurements of ambient temperature, ambient relative humidity, water loss from the PBR, relative humidity of the exhaust gas from the PBR, air flow rate through the PBR, air pressure in the plenum, growth medium temperature, and LED panel temperature were made approximately daily. Measurements show that the growth medium (water) temperature was relatively insensitive to the ambient temperature which varied from 22.7C to 33.6C. The medium temperature ranged from 23.9C (beginning of the test) to 40.6C. The medium temperature mirrored the LED panel temperature staying 2–4C below the LED panel temperature after the first day. The elevated LED panel temperature was likely due to the inefficiency of the LED lights and the fact that much of the light passing through the reactor volume was incident on the LED panel on the opposite side of the reactor. The panels are black in color and absorbed a significant portion of the light passing through the reactor volume. The air flow rate through the PBR ranged from 1.33×10−5m3/s to 1.67×10−5m3/s. The parallel between panel temperature and PBR medium temperature indicate that the amount of air moving through the PBR was insufficient to affect the medium temperature significantly. The heat loss from the PBR to the ambient environment was also small likely due to the small area available to heat loss to the environment when the PBR walls with the LED panels are excluded. The LED panels covered nominally 88% of the PBR reactor volume area. The measured data and measurements of light intensity passing through the two short walls of the panel will be used to estimate heat loss parameters of the PBR. The exhaust air from the PBR varied from 42.6% to 99.1% with the higher measurements occurring days 6–11. Estimates of the energy stored in the algal biomass are also evaluated in the analysis.

Studies on the diversity of substrate composition in the culture medium of Kombucha microorganisms and its influence on the quality of synthesized cellulose

2019 ◽  
Vol 108 ◽  
pp. 21-25 ◽  
Author(s):  
IZABELA BETLEJ
Keyword(s):  
Sorption Capacity ◽  
Growth Medium ◽  
Multilayer Structure ◽  
Culture Medium ◽  
Morphological Structure ◽  
Research Results ◽  
Substrate Composition ◽  
Nitrogen Concentrations

Studies on the diversity of substrate composition in the culture medium of Kombucha microorganisms and its influence on the quality of synthesized cellulose. The paper presents the results of the assessment of the effect of nutrients, specifically different nitrogen concentrations in the growth medium of Kombucha microorganisms, on the morphology of cellulose produced and its sorption capacity. Analyzing the obtained research results, we found that polymers formed in different growth environments differ in morphological structure and swelling index. The polymers synthesized on a nitrogen-rich substrate were characterized by a multilayer structure and a lower swelling index than the polymers obtained on a nutrient-poor substrate

scholarly journals Tearable and Fillable Composite Sponges Capable of Heat Generation and Drug Release in Response to Alternating Magnetic Field

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3637
Author(s):  
Koichiro Hayashi ◽  
Atsuto Tokuda ◽  
Jin Nakamura ◽  
Ayae Sugawara-Narutaki ◽  
Chikara Ohtsuki
Keyword(s):  
Magnetic Field ◽  
Drug Release ◽  
Heat Generation ◽  
Culture Medium ◽  
Surgical Repair ◽  
Thermal Therapy ◽  
Alternating Magnetic Field ◽  
Medium Temperature ◽  
Exogenous Trigger ◽  
Tissue Defects

Tearable and fillable implants are used to facilitate surgery. The use of implants that can generate heat and release a drug in response to an exogenous trigger, such as an alternating magnetic field (AMF), can facilitate on-demand combined thermal treatment and chemotherapy via remote operation. In this study, we fabricated tearable sponges composed of collagen, magnetite nanoparticles, and anticancer drugs. Crosslinking of the sponges by heating for 6 h completely suppressed undesirable drug release in saline at 37 °C but allowed drug release at 45 °C. The sponges generated heat immediately after AMF application and raised the cell culture medium temperature from 37 to 45 °C within 15 min. Heat generation was controlled by switching the AMF on and off. Furthermore, in response to heat generation, drug release from the sponges could be induced and moderated. Thus, remote-controlled heat generation and drug release were achieved by switching the AMF on and off. The sponges destroyed tumor cells when AMF was applied for 15 min but not when AMF was absent. The tearing and filling properties of the sponges may be useful for the surgical repair of bone and tissue defects. Moreover, these sponges, along with AMF application, can facilitate combined thermal therapy and chemotherapy.

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