Sanding of drained peatlands

The results of many years of original research based on soil-ecological monitoring showed the development of degradation processes of previously drained peat soils of the Ryazan Meshchera. Structural amelioration (sanding) of peat soils had some positive effect. The aim of the research was to study the properties of the dried peat soil at reclamation site Tinki-II in the settlement of Polkovo, Ryazan District of Ryazan Region, as a result of sanding. The most common type of German mixed-layer sanding was used in investigations. The methodology was based on the principle of comparing the state of dried peat soil before and after sanding. The content of the less than 0.01 mm fractions was 4.3 %, the rest consisted of particles from 0.25 to 0.05 mm; the pH of the salt extract was 5.7. Mineral fertilizers were applied in the experiment in small quantities: ammonium nitrate – 180 g, superphosphate – 185 g and potassium sulfate – 260 g. The water supply was atmospheric-ground. Variant 2 was more effective – sanding with a dose of 1200 t/ha for sluicing and maintaining GWL = 90 cm, as evidenced by improved water-physical properties of the soil: the total porosity increased by 5 %; the total water capacity decreased from 270 to 70%, that was almost 4 times. Keywords: peat soil, degradation, sluicing, sanding, yield, potato.

Digital monitoring of agro-ecosystems indicators on the basis of space and unmanned technologies

The aim of the research is to develop a digital monitoring methodology for agro-ecosystem indicators based on space and unmanned technologies with the conversion of digital aerial photography results into real parameters of agrophytocenosis indicators as physical units. The paper uses the results of research on digital monitoring of agrophytocenosis of single- and poly-species crops of grain and leguminous crops on the experimental field of the Samara State Agrarian University in 2018 (southern forest-steppe of Trans-Volga region). The soil is a typical medium heavy black loamy chernozem (humus content is 5.9 %, easily hydrolysed nitrogen is 80–120 mg/kg, labile phosphorus is 135–145 mg/kg, exchangeable potassium is 150–195 mg/kg, salt extract pH is 6.9). The research conducted in 2018 revealed that the stated aim is achievable in principle. According to the results of 2018, the greatest correlation between the vegetative biomass index NDVI and grain yield for winter and spring cereal crops was detected at the milky stage of the grains (up to r = 0.82).

The influence of crops on the agrochemical properties of alluvial soils of the central floodplain of the Moksha River

Studies have shown that long-term cultivation of agricultural crops is influenced the agrochemical properties of alluvial soils. The perennial herbs affect the content of humus in the soil. In the sod layer under them is being 6,4–6,8 % of organic matter, in the subsurface – 5,3–5,9 %. Down the profile is reduced to 2.2–3.8 %. With long-term cultivation of potatoes, which after itself contains a little amount of root residues, the processes of decomposition of organic substances in the soil prevail over the processes of their synthesis. depends on Accordingly the content of humus is much lower (0.5–4.6 %). Alluvial soils under crops of the perennial herbs were characterized by neutral and slightly acidic active reaction of soil solution (pH of water extract is 6.5–7.5, pH of salt extract is 5.5–6.8). Under potatoes, the pH of the aqueous extract is reduced to 5,9–6,9, salt to 5,4–6,7, which is probably due to an increase in the pH of the upper horizons as a result of mineral fertilizers. Hydrolytic acidity of floodplain soils is rather low in all variants (1,1–2,7 mg/100 g of soil), it is characterized as neutral. The index of the sum of the exchange bases to a greater extent depends on the particle size distribution. Accordingly, it is high both under the perennial herbs and under potatoes. The studied alluvial soils are almost completely filled with bases. The value of this indicator is quite high: 91-97 %. Moreover, under the perennial herbs the index is 1-3 % in arable and 1-4 % in sub-arable horizons higher than under potatoes. The soils of the floodplain of The Moksha river are low in phosphorus and potassium in all variants of the experiment. Just above these figures were under the potatoes due to greater cultivation. With an increase in the level of agricultural technology, saturation of crop turnover with perennial grasses, a justified system of fertilization, the agrochemical properties of alluvial soils of the Central floodplain of The Moksha river are favorable for the stable harvest yields of vegetable and field crops.

WEED INFESTATION OF WINTER TRITICALE, DEPENDING ON AGRICULTURAL PRACTICES OF CULTIVATION

The investigations were carried out in 2014-2015, on a light gray forest heavy loamy soil. The humus content in the arable layer was 5.9%. The mobile phosphorus was 162 and exchangeable potassium was 172 mg per kilogram, respectively, pH of salt extract was 5.6. The total area of the plot was 200 m2, the discount area - 150 m2. Replication - fourfold, placing of plots was successive. Experience scheme: Factor A - preplant soil tillage: 1. Cultivation - 10-12 cm. 2. Milling – 10-12 cm. Factor B – fertilizers: 1. Without fertilizers (control). 2. Fertilizers, aimed to 4.0 tons per hectare (N120P75K90). The following varieties were used as the object of the study: Kornet, Kristal and Nemchinovskaya 56. The norms of fertilizers is calculated by the balance method, based on their removal from the crop and in the soil. The options, treated with a cultivator, were the most clogged. There are no significant difference among tested varieties of Kornet, Nemchinovskaya 56 and Kristal, but Nemchinovskaya 56 was the most clogged - 36 units per square meter, and less weedy was Cornet. When milling , the most clogged was Nemchinovskaya 56 variety - 30 units per square meter. In the way of without fertilization (control), Kristal variety was the most clogged - 21 units per square meter. The weed infestation of winter triticale at the beginning of growing season of Kristal variety was more to 21.3%, Nemchinovskaya 56 variety to 17% and Kristal to 21.6% more, than in milling. At the end of the growing season of winter triticale during cultivation the weeds increased by 71% and 74%. The same pattern was preserved debris perennial weeds early in the growing season 2.4-4.2 units per square meter, at the end of the growing season 4.6-4.7 units per square meter. The most clogged varieties were Nemchinovskaya 56 – 2.8-4.6 units per square meter and Kristal 2.7-4.7 units per square meter.

Phagocytic and metabolic activity of neutrophils of rats in the early stages of post-embryonic development under the influence of biologically active substances of the salt extract of Hirudo verbana

The nitroblue tetrazolium test reflects the degree of activation of oxygen-dependent mechanisms of bactericidal activity of phagocytotic cells, so the increase in nbt-test and phagocytic indices shows increased activation in the bacterial process and enhanced protection with the help of the absorption ability of the cells in the body. We examined females of nonlinear rats after feeding of their offspring, which 2 weeks before and 2 weeks after mating were injected intraperitoneally with antigens of saline extract of medical leeches (4 injections) and the offspring at days 1, 15, 30, 45, 60. The dosage of salt extract antigens was carried out by determining the protein concentrations using the Lowry protein assay. The animals were immobilized using an immobilizing device. The animals were distributed into three groups: the first research and experimental group of animals was subject to the influence of medical leech salt extract antigens to the amount of 0.5 ml (at the rate of 3 µg/g of animal weight); the second group of intact animals was not subject to intervention, the third control group of animals was administered intraperitoneally saline 0.5 ml of 9% saline solution. We studied females after feeding offspring and offspring at 1, 15, 30, 60 days. The animals were kept in vivarium conditions in individual cages on a standard diet. In the experiment we used 60 females of non-linear rats and 300 of their offspring. All animals were decapitated under ether anesthesia. After that, we took blood, made a solution with crystal heparin and examined the laboratory parameters: total number of leukocytes and leukocyte formula. Evaluation of nonspecific resistance of the organism was investigated using tests which characterize the activity of neutrophils (nbt-test, phagocytosis indices). The results of our studies revealed a positive effect of antigens of saline extract of medical leeches on the absorptive and metabolic activity of neutrophils in the females and their offspring in all study periods compared with the control, an increased number of leukocytes, due to the change of the process of differentiation and deposition of cells in immunogenic organs on proliferation. Changes in immunological parameters indicate the immunostimulatory effect of AG ML on leukopoiesis and on the functional activity of neutrophils – the first line of defense against entry into the body of a variety of bacteria, fungi and protozoa.

Praline metabolism by germinating Lilium longiflorum pollen. I. Labelling of cytoplasmic, wall and culture medium molecules

Radioactivity occurs in trithloroacetic acid (TCA)-soluble and precipitable, cytoplasm and salt-washed walls following germination of <em>Lilium longiflorum</em>, cv. 'Ace' pollen in medium containing [¹⁴C]-proline (Pro). Sephadex gel filtration on G-25 through G-100 was employed to determine whether radioactivity in cytoplasm, wall and growth medium from pollen fed [¹⁴C]-Pro or [³H]=Pro plus [¹⁴C]-arafbinose (Ara) was contained within molecules possessing molecular weights of 5,000 to 100,000 daltones or greater. G-25 elution profiles of a crude cytoplasmic fraction (15,000 X g supernatant) from [¹⁴C]-Pro labelled pollen yielded a radioctive void volume peak and a retarded peak. The void volume peak contained hydroxyproline (Hyp), and exhibited a coincidence of [³H]-Pro and [¹⁴C] -Ara labelling when pollen was double labelled with the two isotopes. This peak also contained radioactivity when pollen was germinated in 2-[³H]-myo-inositol. Germination in medium supplemented with 100 µM 2,2'-dipyridyl eliminated radioactivity from 2-[³H]-myo-inositol or [¹⁴C]-,Pro in the peak. Filtratian on G-25 of aTCA-soluble fraction of a salt-extract of walls from [¹⁴C]-Pro labelled pollen resulted in void volume and two retarded peaks. Void volume and two retarded peaks were also obtained upon G-25 filtration of a cellulase-digest of walls from [M]-Pro labeled pollen. The void volume peak contained Hyp, Lys, Gly, Ala, Ser, Glu and Asp acids, Val, Tyr, Leu or lieu and Pro. Sephadex G-90, 75, and 100 elution profiles of cellulasedigests of walls from [³H]-,Pro and [¹⁴C]-Ara labelled pollen yielded radioactive retarded and Hyp-containing void volume peaks with a coincidence of [³H] and [¹⁴C] labelling. Label in the void volume was obtained when either rhozyme P11- or pepsin-digests of walls from [¹⁴C]-Pro labelled pollen were gel filtered on G-50. Paper electrophoresis coupled with paper chromatography of acid hydrolyzates of salt-washed wall fractions demonstrated 15 of the common amino acids. Gel filtration on G-25 of growth medium in which pollen was germinated resulted in two peaks, one of which eluted in the void volume. contained Hyp and excluded during subsequent gel filtration on G-100.

Characteristics and behaviour of liposomes when incubated with natural bile salt extract: implications for their use as oral drug delivery systems

The use of liposomes for oral administration of drugs and for food applications is based on their ability to preserve entrapped substances and to increase their bioavailability.