Recovery and survival of nontuberculous mycobacteria under various growth and decontamination conditions

1984 ◽  
Vol 30 (9) ◽  
pp. 1112-1117 ◽  
Author(s):  
Robert W. Brooks ◽  
Karen L. George ◽  
Bruce C. Parker ◽  
Joseph O. Falkinham III ◽  
Howard Gruft
Keyword(s):  
Malachite Green ◽  
Mycobacterium Avium ◽  
Antifungal Agent ◽  
Benzalkonium Chloride ◽  
Natural Waters ◽  
Nontuberculous Mycobacteria ◽  
Water Decontamination ◽  
Cell Adsorption ◽  
Soil And Water

The survival of microorganisms of the Mycobacterium avium, M. intracellulare, and M. scrofulaceum (MAIS) complex was evaluated after various soil and water decontamination regimens. Survival was reduced by growing cells in natural waters compared with laboratory media and by inclusion of malachite green in media as an antifungal agent. Decontamination with benzalkonium chloride, while reducing survival significantly less than 1% NaOH, failed to eliminate many fungi. Recovery from soil was further reduced by transfer losses and by irreversible cell adsorption onto particulates.

scholarly journals Avian Mycobacteriosis: Still Existing Threat to Humans

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Michal Slany ◽  
Vit Ulmann ◽  
Iva Slana
Keyword(s):  
Soft Tissue ◽  
Pulmonary Disease ◽  
Mycobacterium Avium ◽  
Nontuberculous Mycobacteria ◽  
Laboratory Diagnosis ◽  
Common Cause ◽  
Wide Range ◽  
Extra Pulmonary ◽  
Diagnosis Of Infection ◽  
Soil And Water

The nontuberculous mycobacteria are typically environmental organisms residing in soil and water. These microorganisms can cause a wide range of clinical diseases; pulmonary disease is most frequent, followed by lymphadenitis in children, skin and soft tissue disease, and rare extra pulmonary or disseminated infections.Mycobacterium aviumcomplex is the second most common cause of pulmonary mycobacterioses afterM. tuberculosis. This review covers the clinical and laboratory diagnosis of infection caused by the members of this complex and particularities for the treatment of different disease types and patient populations.

Intrathoracic Disease Associated With Mycobacterium avium-intracellulare Complex in Otherwise Healthy Children: Diagnostic and Therapeutic Considerations

PEDIATRICS ◽  
1994 ◽  
Vol 94 (5) ◽  
pp. 741-742
Author(s):  
Sandeep K. Gupta ◽  
Ben Z. Katz
Keyword(s):  
United States ◽  
Mycobacterium Avium ◽  
Nontuberculous Mycobacteria ◽  
The United States ◽  
Healthy Children ◽  
Human Pathogen ◽  
Cervical Lymphadenitis ◽  
Cervical Adenitis ◽  
Mycobacterium Avium Intracellulare ◽  
Therapeutic Considerations

Mycobacterium tuberculosis (MTB) is a well described human pathogen.1 Less commonly, atypical or nontuberculous mycobacteria (NTM) can cause disease in humans. Recent studies report that NTM account for one-third of all pathogenic mycobacterial isolates in the United States.2 Mycobacterium avium-intracellulare complex (MAI) is the most common NTM causing human disease.2 It is also the most common mycobacterial cause of cervical lymphadenitis in children in areas with low endemic rates of MTB infection.3 MAI/NTM infection other than cervical adenitis is unusual in children, except in those that are immunosuppressed.4 Rarely, MAI presents as mediastinal or endobronchial disease in otherwise healthy children.

scholarly journals Effective Treatment of Mycobacterium avium subsp. hominissuis and Mycobacterium abscessus Species Infections in Macrophages, Biofilm, and Mice by Using Liposomal Ciprofloxacin

2018 ◽  
Vol 62 (10) ◽  
Author(s):  
James D. Blanchard ◽  
Valerie Elias ◽  
David Cipolla ◽  
Igor Gonda ◽  
Luiz E. Bermudez
Keyword(s):  
Effective Treatment ◽  
Mouse Models ◽  
Mycobacterium Avium ◽  
Nontuberculous Mycobacteria ◽  
Mycobacterium Abscessus ◽  
Content Type ◽  
Number Of Individuals ◽  
Intranasal Instillation

ABSTRACT Nontuberculous mycobacteria (NTM) affect an increasing number of individuals worldwide. Infection with these organisms is more common in patients with chronic lung conditions, and treatment is challenging. Quinolones, such as ciprofloxacin, have been used to treat patients, but the results have not been encouraging. In this report, we evaluate novel formulations of liposome-encapsulated ciprofloxacin (liposomal ciprofloxacin) in vitro and in vivo. Its efficacy against Mycobacterium avium and Mycobacterium abscessus was examined in macrophages, in biofilms, and in vivo using intranasal instillation mouse models. Liposomal ciprofloxacin was significantly more active than free ciprofloxacin against both pathogens in macrophages and biofilms. When evaluated in vivo, treatment with the liposomal ciprofloxacin formulations was associated with significant decreases in the bacterial loads in the lungs of animals infected with M. avium and M. abscessus. In summary, topical delivery of liposomal ciprofloxacin in the lung at concentrations greater than those achieved in the serum can be effective in the treatment of NTM, and further evaluation is warranted.

scholarly journals Management of patients with pulmonary mycobacteriosis in France: a multicenter retrospective cohort study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Pascale Bemer ◽  
Olivia Peuchant ◽  
Hélène Guet-Revillet ◽  
Julien Bador ◽  
Charlotte Balavoine ◽  
...  
Keyword(s):  
Cohort Study ◽  
Prognostic Factors ◽  
Mycobacterium Avium ◽  
Retrospective Cohort Study ◽  
Retrospective Cohort ◽  
Cox Regression ◽  
Nontuberculous Mycobacteria ◽  
Great Variability ◽  
Microbiological Characteristics ◽  
Smear Negative

Abstract Background Recent studies report very low adherence of practitioners to ATS/IDSA recommendations for the treatment of nontuberculous mycobacteria pulmonary disease (NTM-PD), as well as a great variability of practices. Type of management could impact prognosis. Methods To evaluate management and prognosis of patients with NTM-PD cases with respect to ATS recommendations, we conducted a multicenter retrospective cohort study (18 sentinel sites distributed throughout France), over a period of six years. We collected clinical, radiological, microbiological characteristics, management and outcome of the patients (especially death or not). Results 477 patients with NTM-PD were included. Respiratory comorbidities were found in 68% of cases, tuberculosis sequelae in 31.4% of patients, and immunosuppression in 16.8% of cases. The three most common NTM species were Mycobacterium avium complex (60%), M. xenopi (20%) and M. kansasii (5.7%). Smear-positive was found in one third of NTM-PD. Nodulobronchiectatic forms were observed in 54.3% of cases, and cavitary forms in 19.1% of patients. Sixty-three percent of patients were treated, 72.4% of patients with smear-positive samples, and 57.5% of patients with smear-negative samples. Treatment was in adequacy with ATS guidelines in 73.5%. The 2-year mortality was 14.4%. In the Cox regression, treatment (HR = 0.51), age (HR = 1.02), and M. abscessus (3.19) appeared as the 3 significant independent prognostic factors. Conclusion These findings highlight the adequacy between French practices and the ATS/IDSA guidelines. Treatment was associated with a better survival.

scholarly journals Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil

2020 ◽  
pp. e20190184-e20190184
Author(s):  
Caroline Busatto ◽  
Júlia Silveira Vianna ◽  
Ana Barbara Scholante Silva ◽  
Rossana Basso ◽  
Jussara Silveira ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Avium ◽  
Nontuberculous Mycobacteria ◽  
Suspected Tuberculosis ◽  
South Of Brazil

scholarly journals In Vitro Activity of Rifamycin Derivatives against Nontuberculous Mycobacteria, Including Macrolide- and Amikacin-Resistant Clinical Isolates

2021 ◽  
Vol 65 (5) ◽  
Author(s):  
Dae Hun Kim ◽  
Su-Young Kim ◽  
Hee Jae Huh ◽  
Nam Yong Lee ◽  
Won-Jung Koh ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Nontuberculous Mycobacteria ◽  
Therapeutic Option ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
In Vitro Activity ◽  
Content Type ◽  
Resistant Disease

ABSTRACT We evaluated the in vitro activity of rifamycin derivatives, including rifampin, rifapentine, rifaximin, and rifabutin, against clinical nontuberculous mycobacteria (NTM) isolates. Of the rifamycin derivatives, rifabutin showed the lowest MICs against all NTM species, including Mycobacterium avium complex, M. abscessus, and M. kansasii. Rifabutin also had effective in vitro activity against macrolide- and aminoglycoside-resistant NTM isolates. Rifabutin could be worth considering as a therapeutic option for NTM disease, particularly drug-resistant disease.

Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children

2019 ◽  
Vol 38 (6) ◽  
pp. 1113-1122
Author(s):  
Chiara Della Bella ◽  
Elisabetta Venturini ◽  
Savannah Devente ◽  
Paola Piccini ◽  
Simona Tapinassi ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Nontuberculous Mycobacteria

Selective medium for the isolation and enumeration of Mycobacterium avium-intracellulare and M. scrofulaceum

1986 ◽  
Vol 32 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Karen L. George ◽  
Joseph O. Falkinham III
Keyword(s):  
Carbon Source ◽  
Mycobacterium Avium ◽  
Sole Carbon Source ◽  
Natural Waters ◽  
Tween 80 ◽  
Selective Medium ◽  
Selective Isolation ◽  
Mineral Salts ◽  
Mycobacterium Avium Intracellulare

A medium for the selective isolation and enumeration of Mycobacterium avium-intracellulare and M. scrofulaceum (MAIS) was developed, based upon the ability of these mycobacteria to utilize Tween 80 as sole carbon source and grow optimally at pH 5.5 on a simple mineral salts medium. Representative MAIS strains had higher efficiencies of plating on the Tween 80 medium compared with Middlebrook 7H10. It was shown that nonmycobacterial organisms in natural waters had lower efficiencies of plating on the Tween 80 medium and smaller colonies, thus allowing direct isolation and enumeration of the slowly growing mycobacteria without overgrowth.

scholarly journals Novel Administration of Clofazimine for the Treatment of Mycobacterium avium Infection

2020 ◽  
Vol 7 (6) ◽  
Author(s):  
Ethan Valinetz ◽  
Helen Stankiewicz Karita ◽  
Paul S Pottinger ◽  
Rupali Jain
Keyword(s):  
Cystic Fibrosis ◽  
Lung Transplantation ◽  
Mycobacterium Avium ◽  
Nontuberculous Mycobacteria ◽  
Vitro Activity ◽  
The Novel ◽  
In Vitro Activity ◽  
Avium Infection ◽  
Mycobacterium Avium Infection

Abstract Clofazimine has demonstrated in vitro activity against many nontuberculous mycobacteria. We present the case of a woman with cystic fibrosis who developed disseminated macrolide-resistant Mycobacterium avium infection following lung transplantation treated in part with clofazimine. We describe the novel administration of clofazimine via gastrostomy tube.

scholarly journals MTSA-10, the Product of the Rv3874 Gene of Mycobacterium tuberculosis, Elicits Tuberculosis-Specific, Delayed-Type Hypersensitivity in Guinea Pigs

2000 ◽  
Vol 68 (2) ◽  
pp. 990-993 ◽  
Author(s):  
Roberto Colangeli ◽  
John S. Spencer ◽  
Pablo Bifani ◽  
Alan Williams ◽  
Konstantin Lyashchenko ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Mycobacterium Avium ◽  
Guinea Pigs ◽  
Nontuberculous Mycobacteria ◽  
Specific Antigen ◽  
Delayed Type Hypersensitivity ◽  
New Skin

ABSTRACT In a search for new skin test reagents specific for tuberculosis, we found that the antigen encoded by gene Rv3874 of Mycobacterium tuberculosis elicited delayed-type hypersensitivity in M. tuberculosis-infected guinea pigs but not in control animals immunized with Mycobacterium bovis bacillus Calmette-Guérin (BCG) or Mycobacterium avium. The antigen, which was named MTSA-10 (for M. tuberculosis-specific antigen 10), is a prime candidate for a component of a new tuberculin that will allow discrimination by a skin test of latent M. tuberculosis infection from vaccination with BCG or from sensitization with environmental, nontuberculous mycobacteria.

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