Pleurotus ostreatus requirements for P, K, Mg, and Mn in submerged culture

1988 ◽  
Vol 34 (5) ◽  
pp. 620-624 ◽  
Author(s):  
Wireko Manu-Tawiah ◽  
Antonio M. Martin
Keyword(s):  
Pleurotus Ostreatus ◽  
Submerged Culture ◽  
Mycelial Growth ◽  
Synthetic Medium ◽  
Potassium Phosphate ◽  
Biomass Concentration ◽  
Linear Relationships ◽  
Dry Biomass ◽  
The Media ◽  
Extract Medium

The nutritional requirements of P, K, Mg, and Mn for the mycelial growth of the fungus Pleurotus ostreatus have been studied. Experiments were conducted in submerged culture using a synthetic medium and a peat-extract-based medium. Requirements for both additional phosphorus and additional potassium were detected, and supplementing the peat extract medium with potassium phosphate enhanced the mycelial growth. The addition of yeast extract and potassium phosphate, combined with the peat extract, produced the highest dry biomass concentration in this work, 6.8 g/L. Linear relationships were found between the dry biomass concentration of P. ostreatus and the concentration of KH2PO4 added to the media. No additional requirement for magnesium beyond that existing in the peat extract medium was detected. Supplementing this medium with manganese in the range of 0.1 to 0.15 g/L produced a slight increase in the growth.

Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture

2010 ◽  
Vol 50 (3) ◽  
pp. 131-138 ◽  
Author(s):  
Lefki-Maria Papaspyridi ◽  
Petros Katapodis ◽  
Zacharoula Gonou-Zagou ◽  
Evangelia Kapsanaki-Gotsi ◽  
Paul Christakopoulos
Keyword(s):  
Biomass Production ◽  
Pleurotus Ostreatus ◽  
Submerged Culture ◽  
Dietary Fibres

scholarly journals Effect of Lactose on Soluble-glucan Production and on the Ultrastructure of Sclerotium rolfsii Sacc. Grown in Submerged Culture

Microbiology ◽  
2000 ◽  
Vol 81 (1) ◽  
pp. 145-149 ◽  
Author(s):  
Y. Okon ◽  
I. Chet ◽  
Naomi Kislev ◽  
Y. Henis
Keyword(s):  
Carbon Source ◽  
Submerged Culture ◽  
Sole Carbon Source ◽  
Synthetic Medium ◽  
Sclerotium Rolfsii ◽  
Dry Weight ◽  
Solid Media

SUMMARY A significant increase in mycelial dry weight and a decrease in production of extracellular glucan were observed when lactose was added to a submerged culture of Sclerotium rolfsii grown in a liquid glucose-synthetic medium. When added as a sole carbon source, lactose at 2·5% (w/v) induced the formation of dark, spherical, compact bodies. The ultrastructure of these bodies is compared with that of sclerotia formed on solid media.

Effects of different growth conditions on enzyme production by Pleurotus ostreatus in submerged culture

1992 ◽  
Vol 42 (2) ◽  
pp. 89-94 ◽  
Author(s):  
G. Burla ◽  
A.M. Garzillo ◽  
M. Luna ◽  
L. Ercoli Cardelli ◽  
A. Schiesser
Keyword(s):  
Pleurotus Ostreatus ◽  
Submerged Culture ◽  
Enzyme Production ◽  
Growth Conditions

Repair of salt tolerance and recovery of lost D-alanine and magnesium following sublethal heating of Staphylococcus aureus are independent events

1981 ◽  
Vol 27 (6) ◽  
pp. 627-632 ◽  
Author(s):  
A. Hurst ◽  
A. Hughes
Keyword(s):  
Staphylococcus Aureus ◽  
Salt Tolerance ◽  
Phosphate Buffer ◽  
Synthetic Medium ◽  
Potassium Phosphate ◽  
Complex Media ◽  
Rich Medium ◽  
Potassium Phosphate Buffer ◽  
Heat Damage ◽  
Independent Events

Sublethal heating of Staphylococcus aureus S6 in potassium phosphate buffer caused loss of salt tolerance, D-alanine, and magnesium. During incubation in rich complex media all three of the damaged sites were repaired. Repair occurred more slowly but went to completion in a dilute synthetic medium (DSM), free of D-ala. DSM plus penicillin or D-cycloserine allowed repair of salt tolerance but recovery of normal levels of D-ala or Mg was prevented. When DSM-repaired cells were cultured into fresh rich medium they grew rapidly after a short lag. Cells which had acquired their salt tolerance in DSM plus cycloserine and were D-ala and Mg deficient grew slowly and had a lag of 3 h. We suggest that heat damage has two separate primary targets in S. aureus cells: the membrane, which is manifested by loss of salt tolerance, and a second site, possibly teichoic acids, manifested by loss of D-ala and Mg.

scholarly journals Mycelial growth and exo-biopolymer production by submerged culture of various edible mushrooms under different media

2002 ◽  
Vol 34 (1) ◽  
pp. 56-61 ◽  
Author(s):  
S.W. Kim ◽  
H.J. Hwang ◽  
J.P. Park ◽  
Y.J. Cho ◽  
C.H. Song ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Edible Mushrooms

scholarly journals Biological Activities of the Polysaccharides Produced in Submerged Culture of Two EdiblePleurotus ostreatusMushrooms

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Emanuel Vamanu
Keyword(s):  
Antioxidant Activity ◽  
Pleurotus Ostreatus ◽  
Submerged Culture ◽  
Biological Activities ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Carbohydrate Analysis ◽  
Chelating Activity ◽  
Batch System

Exopolysaccharides (EPS) and internal (intracellular) polysaccharides (IPS) obtained from thePleurotus ostreatusM2191 and PBS281009 cultivated using the batch system revealed an average of between 0.1–2 (EPS) and 0.07–1.5 g/L/day (IPS). The carbohydrate analysis revealed that the polysaccharides comprised 87–89% EPS and 68–74% IPS. The investigation of antioxidant activityin vitrorevealed a good antioxidant potential, particularly for the IPS and EPS isolated from PBS281009, as proved by the EC50value for DPPH, ABTS scavenging activity, reducing power, and iron chelating activity.

Optimization of Culture Conditions on Mycelial Grown in Submerged Culture of Cordyceps militaris

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jian Dong Cui ◽  
Li Qiang Yuan
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Fold Increase ◽  
Culture Conditions ◽  
Statistical Experimental Design ◽  
Fermentation Time ◽  
Original Culture ◽  
Production Values ◽  
Validation Experiment ◽  
Optimal Region

Statistical experimental design strategy (SES) was employed to optimize the culture conditions for the mycelial growth of C. militaris by submerged culture. Using Plackett-Burman design (PBD), liquid volumes in flask and fermentation time were identified as the significant variables that would highly influence mycelial growth, and these variables were subsequently optimized using response surface method (RSM). The steepest ascent method was used to access the optimal region of the culture condition. The results showed that the optimum values of the tested variables were 47.3 mL of liquid volumes in flask and 7.6 days of fermentation time. Under optimized culture conditions, the mycelial production was enhanced from 10.33 to 19.97 g/L. In comparison with that of original culture conditions, 1.9-fold increase was obtained. The validation experiment showed that the experimentally determined production values were in close agreement with the statistically predicted ones.

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