Antifungal Activity of Plant Extracts against Candida albicans

1997 ◽  
Vol 25 (02) ◽  
pp. 181-184 ◽  
Author(s):  
Cristina Perez ◽  
Cristina Suarez
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Plant Extracts ◽  
Folk Medicine ◽  
Opportunistic Pathogen ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Micro Organisms ◽  
Agar Well Diffusion Method

In previous papers, we reported the antimicrobial activity of plants used in Argentine folk medicine against different micro-organisms. The present study deals with the screening of 11 of these plants against the opportunistic pathogen fungus Candida albicans. Aqueous extracts 6% p/v (6 mg dry plant per 100 ml of water) were checked against fungus cultures by the agar-well diffusion method. Five extracts showed antifungal activity.

scholarly journals In Vitro Antibacterial and Antifungal Activity and Datura Innoxia Extracts

2014 ◽  
Vol 3 (3) ◽  
pp. 173-185
Author(s):  
Adam IY Shama ◽  
YM Abd-Kreem ◽  
AA Fadowa ◽  
RM Samar ◽  
MK Sabahelkhier
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Antibacterial Drug ◽  
Aqueous Extracts ◽  
Datura Innoxia ◽  
Methanolic Extracts ◽  
Leaves And Roots

The aim of this study was evaluated the Antimicrobial Activity of extraction of Datura innoxia (Seeds, leaves and roots). Datura innoxia Seeds, leaves and roots were collected to examine their antimicrobial activity. Extracts of different parts of the plant were tested against standard microorganisms by using the agar- well diffusion method. Extracts of methanol, and aqueous of seeds, leaves and roots were prepared and tested against four types of bacteria namely: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris and two types of fungi namely: Aspergillus niger and Candida albicans. The methanolic and aqueous extracts of leaves showed high activities against fungi (A. niger) and less effect on the all bacteria. The methanolic extracts of seeds showed high activities against all organisms except fungi (C. albicanas), while the aqueous extracts of seeds showed no activity on the bacteria. All organisms were examined against known standard antibiotics and then compare the results of plant extracts with standard antibiotics. The results indicated that the antibacterial drug is less active than the plant extracts, while the antifungal drugs are more active than the plant extracts. DOI: http://dx.doi.org/10.3126/ije.v3i3.11077 International Journal of Environment Vol.3(3) 2014: 173-185

scholarly journals Phytochemical Screening and in vitro Antimicrobial Activity of Cyperus rotundus Leaf Extracts

2021 ◽  
Vol 4 (1) ◽  
pp. 43-52
Author(s):  
EO Agbo ◽  
JO Ogenyi ◽  
CA Adah ◽  
CT Agber
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Methanol Extract ◽  
Cyperus Rotundus ◽  
Diffusion Method ◽  
Methanol Extracts ◽  
Study Results

Cyperus rotundus, a member of family Cyperaceae is a perennial, stoloniferous herb. The study investigated the secondary metabolites present in the leaf of Cyperus rotundus and antimicrobial activities of the hexane, ethyl acetate and methanol extracts. Phytochemical analysis revealed the presence of saponins, alkaloids, phenolic compounds, tannins, steroids, flavonoids, cardiac glycosides and terpenoids. The qualitative test of the extracts show the presence of steroid only in the hexane extract; flavonoid, cardiac glycoside in both the ethyl acetate and methanol extracts; saponin and tannin and phenolics in just the methanol extract. While alkaloid was present in all the extracts, terpenoid was absent in all. Antimicrobial activity of plant extracts were monitored using the agar disk-diffusion method against strains gram-positive bacterial: Staphylococcus aureus, Pseudomonas aeruginosa; and gram-negative bacteria: Escherichia coli, Salmonela typhi, and strains of fungi: Candida albicans and Aspergillus niger. The antimicrobial activity of the extracts was monitored at different concentrations of 50, 25, 12.5 and 6.25 mg/mL prepared from the stock concentration (100mg/mL) by serial dilution; with standard drugs: ciprofloxacin and fluconazole. The inhibition of the test organisms were concentration dependent. The methanol extract showed significantly higher Inhibition Zone (IZ) and Activity Index (AI) against the microbes at all concentrations compared to the ethyl acetate and hexane extracts due to the much phytochemicals (saponins, tanins, phenols, flavonoids, cardiac glycocides and alkaloids) found present in its extract. The overall study results signify the potential of Cyperus rotundus as a source of therapeutic agents. The methanol extract showed the best activity against both bacterial and fungal pathogens at the highest concentration of 100 mg/mL, especially the most susceptible bacteria: Staphylococcus aureus (IZ of 25.0±1.5; AI of 0.96) and Candida albicans (IZ of 25.5±0.5; AI of 0.91). At MIC, MBC and MFC of 6.25 mg/mL and 12.5 mg/mL, the plant extracts showed effective bactericidal and fungicidal effect against the test microbes.

scholarly journals Evaluation of Antibacterial Properties of Different Leaf Extracts of Hyptis suaveolens (L) Poit

2021 ◽  
pp. 40-44
Author(s):  
B. A. Erinle ◽  
A. O. Ajayi ◽  
O. T. Osuntokun
Keyword(s):  
Antimicrobial Activity ◽  
Folk Medicine ◽  
Antibacterial Properties ◽  
Extraction Process ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Hyptis Suaveolens

Aim: Hyptis suaveolens is well known for its immense medicinal properties and are beneficial as folk medicine. The objective of the study is to investigate the efficacy of the leaves for it antimicrobial activity. Material and Methods: The leaves of Hyptis suaveolens was  subjected to extraction process using n-hexane, ethanol and distilled water as solvents and the antimicrobial activity was analyzed against different bacterial strains viz.; Escherichia coli, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Enterococcus faecalis by agar well diffusion method. Results: Aqueous extracts showed inhibitory effect against the different tested bacteria organisms with variable zone of inhibitory range 6 -21 mm. Conclusion: The aqueous extracts of Hyptis suaveolens exhibited the presence of highly effective bio-active compounds in these extracts. These can further be evaluated and characterize to improve upon what has been done to create a novel compound that can be useful for various medicinal purposes.

scholarly journals Evaluation of The Antifungal Activity of Nasturtium officinale (watercress) Oil with Calcium Hydroxide against Candida Albicans Isolated from Root Canal

2021 ◽  
Vol 33 (4) ◽  
pp. 1-5
Author(s):  
Mustafa W Abdul Kareem ◽  
Zainab A Al Dhaher
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Antimicrobial Agents ◽  
Agar Plate ◽  
Diffusion Method ◽  
Nasturtium Officinale ◽  
Oil Extract ◽  
Agar Well Diffusion Method

Background: The interest in herbal extracts as antimicrobial agents has increased over the past few years in endodontic therapy. Nasturtium officinale (watercress) is a promising plant with great medicinal values. This study aimed to investigate the antifungal activity of watercress oil in combination with calcium hydroxide against Candida albicans as intracanal medicament. Materials and Methods: Candida albicans was isolated from patients with necrotic root canal or failed root canal treatment. The sensitivity of Candida albicans to different concentrations of watercress oil extract was determined by using the agar well diffusion method in comparison with calcium hydroxide paste. The agar plate method was used to determine the minimum fungicidal concentration (MFC) of the tested oil against the fungus. The combination of the oil extract of Nasturtium officinale with calcium hydroxide was evaluated and compared to calcium hydroxide paste with iodoform by using the agar well diffusion method. Results: The oil extract exhibited antifungal activity against Candida albicans, this activity was found to be increased as the concentration of extract increased. The tested combination of watercress oil extract with calcium hydroxide revealed larger inhibition zones than the ones formed by each tested agent individually. Conclusion: The oil extract of Nasturtium officinale is active against Candida albicans suggesting its potential to be used as an intracanal medicament alone or in combination with calcium hydroxide.

scholarly journals Comparison of Antimicrobial Activity ofAllium sativumCloves from China and Taşköprü, Turkey

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Ali Yetgin ◽  
Kerem Canlı ◽  
Ergin Murat Altuner
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

In this study, antimicrobial activities of two different samples ofAllium sativumL. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species ofBacillus,Enterobacter,Enterococcus,Escherichia,Klebsiella,Listeria,Pseudomonas,Salmonella, andStaphylococcuswere used for testing antibacterial activity. In addition, antifungal activity againstCandida albicanswas also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.

scholarly journals Evaluation of antimicrobial activity of plant extracts from Brazilian savanna on cariogenic cocci

2017 ◽  
Vol 6 (4) ◽  
Author(s):  
Karina Gerhardt Bianco ◽  
Ellen Cristina Gaetti-Jardim ◽  
Fernanda Lourenção Brighenti ◽  
Elerson Gaetti-Jardim Júnior
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Control Plant ◽  
Brazilian Savanna ◽  
Diffusion Method ◽  
Dilution Method ◽  
Aqueous Extracts ◽  
Initial Screening ◽  
Kill Curve ◽  
Time Kill

This study evaluated the antimicrobial activity of 138 aqueous extracts of 23 plants from Brazilian savanna on cariogenic cocci. Aqueous extracts were prepared from leaves, stems and barks of the plants. The initial screening of antimicrobial activity was performed by the agar diffusion method. Minimal inhibitory concentration (MIC) and minimal bactericidal concentrations were determined using broth dilution method. Time-kill curve were also determined on cells in suspension. The initial screening of antimicrobial activity evidenced that leaves extracts from M. ilicifolius, P. americana, J. cuspidifolia and bark extracts of P. rotundifolia were active on some strains but not all tested microorganisms. Only leaves, bark and stems extracts of M. urundeuva and P. cattleianum were able to inhibit all tested strains. The inhibitory activities of M. urundeuva and P. cattleianum were similar. In general, MBCs were four up to thirty two times higher than MICs. Time-kill curve of the extracts from P. cattleianum and M. urundeuvashowed that a log reduction of the planktonic bacterial inoculum was achieved within 60 minutes of contact.Descriptors: Bacteria; Prevention & Control; Plant Extracts; Dental Caries.

scholarly journals Antifungal activity of some medicinal plant extracts against some fungal isolates

2019 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
Berivan A. Abdullah ◽  
Diana T. Salih ◽  
Asia A. M. Saadullah
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Aloe Vera ◽  
Diffusion Method ◽  
Plant Materials ◽  
Fungal Isolates ◽  
Mic Value

Introduction: Fungi live everywhere in the environment, most of them are not dangerous, but some types of fungi can be harmful to humanhealth. The medicinal plants contain many antimicrobial components that make them recently used as powerful drugs. The aim of the present investigation was to examine the antifungal potential and minimum inhibitory concentration (MIC) of three plant extract: Aloe vera gel, cinnamon (Cinnamomum zeylanicum) and turmeric (Curcuma longa) against three fungal species: Aspergillus niger, Candida albicans and Fusarium oxysporum. Materials and Methods: The plant materials were extracted using solvents DMSO and ethanol and then were tested against the selected fungal isolates using well diffusion method. Results and Discussion: Antifungal activity of Aloe vera against Aspergillus niger showed MIC value of 25% whereas for Candida albicans and Fusarium oxysporum the MIC obtained was 100%. Both cinnamon and turmeric showed maximum potency against Aspergillus niger, Candida albicans and Fusarium oxysporum at highest MIC value of 100 %. The degree of inhibition increased correspondingly with increasing concentrations of the plant extracts. Conclusions: the tested plant extracts have an antifungal activity and could be used as alternative drugs.

Therapeutic efficacy of some extracts of hot pepper fruits (Capsicum annuum) and Sumac seeds (Rhus coriaria) on the growth of some isolated dermatophytes species

2018 ◽  
Vol 1 (3) ◽  
pp. 52-62
Author(s):  
Sara Omran ◽  
Abdulghani Alsamarai ◽  
Firas Razzzaq
Keyword(s):  
Antifungal Activity ◽  
Fungal Infection ◽  
Aqueous Extract ◽  
Plant Extracts ◽  
Therapeutic Efficacy ◽  
Diffusion Method ◽  
Quantitative Detection ◽  
Hot Pepper ◽  
Alcoholic Extract ◽  
Dermatology Clinic

Background: Fungal infections are one of the common skin diseases with difficulty in their treatment approach. The present efficient drugs for fungal infection are limited. Aim: To determine the therapeutic efficacy of plant extracts as alternative antifungal agents. Materials and methods: 100 clinical samples [68 from female and 32 from male] were collected during the period from March to July 2017 from subjects attending Dermatology Clinic in Salah Uldean General Hospital. Fungal infection was diagnosed with using KOH wet preparation. Fungal species identified by using conventional approach. The active ingredients existing in the plant extracts were detected and analyzed through qualitative and quantitative detection technique of chemical compounds using a high performance liquid chromatographic device (HPLC). Agar diffusion method was used to determine antifungal activity of plant extracts. Results: Direct microscopic examination showed that there were (75%) positive samples, while culture shows (67%) positive samples. The isolated dermatophytes belong to Epidermophyoton, Microsporum, and Trichophyton genus. The predominant dermatophytes were T. rubrum (25%) species. The highest frequency of infection was in the age group of 11-20 years. The sensitivity of the tested fungi to the aqueous and alcoholic plant extracts varies. Alcoholic extract of the hot pepper plant was more effective as antifungal than the aqueous extract of the same plant. However, aqueous hot pepper extracts was more effective against T. mentagrophyte than that of alcoholic extract. Additionally, alcoholic Sumac extract shows higher efficacy that aqueous extract. Conclusion: Hot pepper and Sumac extracts show antifungal activity against Microsporum canis, Trichophyton rubrum and T. mentagrophyte.

The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

2019 ◽  
Vol 18 (5) ◽  
pp. 262-274
Author(s):  
E. Benyagoub ◽  
N. Nabbou ◽  
S. Boukhalkhel ◽  
I. Dehini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Fusidic Acid ◽  
Quercus Robur ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

Complexes of Cu (II) with a-Ketoglutaric Acid and 1- (o-tolyl) Biguanide Synthesis, characterization and biological Activity

2019 ◽  
Vol 70 (10) ◽  
pp. 3603-3610
Author(s):  
Madalina Mihalache ◽  
Cornelia Guran ◽  
Aurelia Meghea ◽  
Vasile Bercu ◽  
Ludmila Motelica ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Biological Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Copper Complexes ◽  
Substrate Adhesion ◽  
Inert Substrate

The three copper complexes having a-ketoglutaric acid (H2A) and 1- (o-tolyl) biguanide (TB) ligands have been synthesized and characterized. The proposed formulas for these complexes are: [Cu(TB)(HA)]Cl (C1), [Cu(TB)(HA)CH3COO]�H2O (C2) and [Cu(TB)(HA)](NO3) (C3) where HA represents deprotonated H2A. The complexes obtained were tested for antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853, antifungal activity on Candida albicans ATCC 10231 and antitumor activity on HeLa tumor cells. Due to the antitumor, antifungal, antimicrobial activity and inhibition of inert substrate adhesion, complexes synthesized could be used for potential therapeutic applications.

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