Functionalized Silver Nanoparticles Enhance Therapeutic Effect of Paclitaxel for Prostate Cancer Therapy by Arresting the Cellular Cycle and Producing ROS

NANO ◽  
2021 ◽  
Author(s):  
Jiahong Chen ◽  
Shumin Fang ◽  
Lifen Yang ◽  
Xiaohui Ling ◽  
Jinxian Liao ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Silver Nanoparticles ◽  
Hyaluronic Acid ◽  
Cell Model ◽  
Average Particle Size ◽  
Average Particle ◽  
Dependent Manner ◽  
Lncap Cells ◽  
Ag Nps ◽  
Anticancer Efficacy

Chemotherapy has been proven to be an efficient strategy for the treatment of prostate cancer (PC). However, the insufficient cellular uptake of drugs is the biggest challenge for PC therapy. Therefore, in order to address the above concern, we developed tumor-targeted hyaluronic acid-silver nanoparticles (HA-Ag NPs), in which hyaluronic acid can specially bind with its receptor CD44 overexpressed on LNCaP cells. HA-Ag NPs have been used to load paclitaxel (PTX) to prepare functionalized silver nanoparticles (HA-Ag-PTX). The average particle size of HA-Ag-PTX was about 70 nm and kept under 120 nm for eight days. androgen-sensitive prostate cancer cells (LNCaP) cells show good uptake of HA-Ag-PTX, and HA-Ag-PTX enters LNCaP cells in a clathrin-mediated energy-dependent manner. HA-Ag-PTX exhibits an excellent pH response release of PTX under the acidic condition, which is beneficial to enhance the distribution of PTX in the tumor microenvironment. HA-Ag-PTX effectively suppresses the migration and proliferation of LNCaP cells probably by arresting the cellular cycle at [Formula: see text]0/[Formula: see text]1 phase and enhancing the production of the reactive oxygen species. It is worth mentioning that HA-Ag-PTX exhibits greater anticancer activity in comparison with free PTX in the LNCaP cell model, indicating that HA-Ag NPs can effectively enhance the anticancer efficacy of PTX. As a whole, these findings suggest that HA-Ag-PTX has promising potential for PC therapy.

scholarly journals Biosynthesis and Characterization of Silver Nanoparticles from Bitter Melon (Momordica charantia) Fruit and Seed Extract and their Antimicrobial Activity

2021 ◽  
Vol 2 (1) ◽  
pp. 1-11
Author(s):  
Umme Ruman ◽  
Poonah Kia
Keyword(s):  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Antibacterial Properties ◽  
Seed Extract ◽  
Food Preservation ◽  
Momordica Charantia ◽  
Average Particle ◽  
Ag Nps ◽  
Fruit Extract ◽  
Biotechnological Approach

Momordica charantia is a phenolic rich vegetable. In this study, the fruits and seeds extract of M. charantia were used to synthesize silver nanoparticles (Ag NPs) using biotechnological approach. Structural, morphological, and antimicrobial properties of the synthesized Ag-NPs were characterized using UV/Vis Spectrophotometry, Dynamic Light Scattering (DLS), High Resolution Transmission Electronics Microscopy (HRTEM), Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray diffraction (XRD). In DLS, the average particle size of Ag-NPs was found 17.5 ± 2.1 nm and 18.3 ± 1.9 nm using seed and fruit extract, respectively. HRTEM has revealed their spherical structure for both seed and fruit extract of M. charantia. FESEM images found Ag-NPs with the size between ~20 and ~35 nm. The Ag NPs exhibited Surface Plasmon Resonance (SPR) centered at 405 nm for seed extract and 402 nm for fruit extract using a UV–visible spectrophotometer. FT-IR results showed phenolic and carbohydrate compounds involved in the synthesis of the Ag NPs. Furthermore, the synthesized Ag NPs has found highly rich in antibacterial properties against Escherichia coli and Pseudomonas aeruginosa bacterium. Thus, bioconversion of Ag NPs by M. charantia could be employed as a potential antibacterial source to eliminate pathogenic microorganisms from agricultural and food preservation industry.

scholarly journals Green synthesis, characterization and photocatalytic applications of silver nanoparticles using Diospyros lotus

2020 ◽  
Vol 9 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Samaira Yasmin ◽  
Shazia Nouren ◽  
Haq Nawaz Bhatti ◽  
Dure Najaf Iqbal ◽  
Shan Iftikhar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Average Particle Size ◽  
Xrd Analysis ◽  
Research Area ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
Leaf Extracts ◽  
Diospyros Lotus

AbstractCurrently, the green route for synthesis of silver nanoparticles (Ag NPs) using plants leaf extract is an emerging research area in nanotechnology. The present study was explored for synthesis, characterization and catalytic application of Ag NPs using Diospyros lotus fresh leaf extracts. Factors affecting the synthesis were optimized and optimum conditions were pH of 8.6, silver nitrate (AgNO3) concentration of 1.5 mM and 10 mL leaf extract. Formation of Ag NPs was observed by change in color of reaction mixture from pale yellow to reddish brown. The synthesized Ag NPs were characterized using UV-Vis spectrophotometer, EDX, XRD and SEM analyses. UV-Vis spectrophotometer showed maximum absorbance peak in the range of 407 nm at different time intervals indicating formation of Ag NPs. SEM and XRD analysis confirmed face centered cubic structure and crystalline nature of biologically synthesized Ag NPs with average particle size of 27 nm. The purity of synthesized Ag NPs was revealed by EDX. Finally, photo catalytic activity (PCA) of Ag NPs was studied and 72.91% decolorization of industrial waste water was obtained at 54 h. Some important parameters like pH, turbidity, conductance; TSS, TDS, sulphide, sulphates, etc. were also monitored before and after treatment with Ag NPs.

Biosynthesis of silver nanoparticles by usingGanoderma-mushroom extract

2015 ◽  
Vol 29 (06n07) ◽  
pp. 1540047 ◽  
Author(s):  
S. U. Ekar ◽  
Y. B. Khollam ◽  
P. M. Koinkar ◽  
S. A. Mirji ◽  
R. S. Mane ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Average Particle ◽  
Medicinal Mushroom ◽  
Ag Nps ◽  
Transmission Electron ◽  
Uv Visible

Present study reports the biochemical synthesis of silver nanoparticles ( Ag -NPs) from aqueous medium by using the extract of medicinal mushroom Ganoderma, as a reducing and stabilizing agents. The Ag -NPs are prepared at room temperature by the reduction of Ag+to Ag in aqueous solution of AgNO3. The resultant particles are characterized by using UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM) measurement techniques. The formation of Ag -NPs is confirmed by recording the UV-visible absorption spectra for surface plasmon resonance (SPR) where peak around 427 nm. The prominent changes observed in FTIR spectra supported the reduction of Ag+to Ag . The morphological features of Ag -NPs are evaluated from HRTEM. The spherical Ag -NPs are observed in transmission electron microscopy (TEM) studies. The particle size distribution is found to be nearly uniform with average particle size of 2 nm. The Ag -NPs aged for 15, 30, 60 and 120 days showed no profound effect on the position of SPR peak in UV-visible studies, indicating the protecting/capping ability of medicinal mushroom Ganoderma in the synthesis of Ag -NPs.

scholarly journals Chemical Synthesis of Silver Nanoparticles Using Tri-sodium Citrate, Stability Study and Their Characterization

2020 ◽  
pp. 37-50
Author(s):  
Prem Santhi Yerragopu ◽  
Sharanagouda Hiregoudar ◽  
Udaykumar Nidoni ◽  
K. T. Ramappa ◽  
A. G. Sreenivas ◽  
...  
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Sodium Citrate ◽  
Structural Characteristics ◽  
Average Particle Size ◽  
Stability Study ◽  
Average Particle ◽  
Ag Nps ◽  
Stirring Time ◽  
Absorbance Peak

The present work was aimed to study the synthesis of silver nanoparticles (Ag NPs) using Tri-Sodium Citrate (TSC), stability study of synthesized Ag NPs and their characterization. Synthesis of Ag NPs has been carried out by maintaining different conditions such as TSC concentration (0.50, 1.00 and 1.50%), AgNO3 concentration (0.50, 1.00 and 1.50 mM) and stirring time (10, 15 and 20 min). The stability study of synthesized Ag NPs conducted for 30 days without adding any stabilizing agents. The characterization of synthesized Ag NPs was carried out for different parameters like particle size and zeta potential using particle size analyzer, absorbance peak by UV-Visible spectrophotometer, morphology by Scanning Electron Microscope (SEM), crystallinity by X-Ray Diffractometer (XRD) and material structural characteristics by Atomic Force Microscope (AFM). The stable chemically synthesized Ag NPs were obtained at C20 (AgNO3 concentration of 1.5 mM, TSC 1.5% and stirring time 20 min) (desirability 99.97%), with average particle size of 22.14 nm and average absorbance peak of 449.85 nm.

scholarly journals Characterization of Silver Nanoparticles Synthesized Using Ocimum basilicum Seed Extract

2021 ◽  
Vol 11 (2) ◽  
pp. 3411-3420
Keyword(s):  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Morphological Characteristics ◽  
Ocimum Basilicum ◽  
Seed Extract ◽  
Average Particle ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
X Ray ◽  
Dpph Method

The development of an eco-friendly method for the production of nanomaterials is an area of significant research and commercial interest owing to its numerous applications in various disciplines. This study used a simple green synthesis approach to produce silver nanoparticles using Ocimum basilicum seed extract. The optical, structural, and morphological characteristics of the synthesized silver nanoparticles (Ag NPs) were found using UV visible absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-Ray diffractometer (XRD), dynamic light scattering (DLS), field emission scanning electron microscope (FE-SEM), and energy dispersive X-ray analysis (EDAX). The aqueous medium containing reduced silver ions showed maximum UV absorption at 430 nm in response to the plasmon absorbance behavior of silver nanoparticles. Synthesized nanoparticles had a spherical shape with an average particle size of 134.04 nm and a face-centered cubic (FCC) structure. Synthesized Ag NPs showed excellent free radical scavenging activity as evaluated using the DPPH method. Additionally, the green synthesized silver nanoparticles showed appreciable antimicrobial activity against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria compared to O. basilicum seed extract. Thus, O. basilicum seed extract can be used as a bio-reducing agent for producing silver nanoparticles and an effective way of using bioactive resources with their medicinal benefits.

scholarly journals GREEN SYNTHESIS OF SILVER NANOPARTICLES USING GRAPE POMACE WASTE FOR THE DEGRADATION OF PHARMACEUTICALS DRUGS AND ORGANIC DYES

2019 ◽  
pp. 37-42
Author(s):  
Margarita Skiba ◽  
Viktoria Vorobyova ◽  
Oleksandr Pivovarov
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Metallic Nanoparticles ◽  
Average Particle Size ◽  
Silver Ions ◽  
Grape Pomace ◽  
Chemical Extraction ◽  
Average Particle ◽  
Ag Nps ◽  
Visible Absorption

The green synthesis of metallic nanoparticles paved the way to improve and protect the environment bydecreasing the use of toxic chemicals. A simple and eco-friendly method for silver nanoparticles (AgNPs) synthesis employing the aqueous extract obtained from grape pomace by plasma-chemical extraction technique was developed. The reduction of silver ions in solution was monitored using UV–visible absorption spectroscopy. The synthesised nanoparticles were characterised using scaning electron microscopy (SEM) and dynamic light scattering measurement (DLS). The sizes of the spherical silver particles were found to be in the range of 27–33 nm. The effect of silver ions concentrations on the synthesis of silver nanoparticles and average particle size was investigated. As-prepared Ag NPs had an excellent catalytic activity as a catalyst for the degradation of ibuprofen, which was carried out in 50 s. The current findings are equally extendable for safeguarding the aquatic environment against the pollution caused by drugs and microbial activity via a facile, highly economical, rapid and efficient reduction/degradation method based on the catalytic potential of Ag NPs. The report emphasizes the effect of the size of silver nanoparticles on the degradation rate of hazardous dyes - methyl blue by NaBH4..

scholarly journals Biofabricated Fatty Acids-Capped Silver Nanoparticles as Potential Antibacterial, Antifungal, Antibiofilm and Anticancer Agents

2021 ◽  
Vol 14 (2) ◽  
pp. 139
Author(s):  
Mohammad Azam Ansari ◽  
Sarah Mousa Maadi Asiri ◽  
Mohammad A. Alzohairy ◽  
Mohammad N. Alomary ◽  
Ahmad Almatroudi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Silver Nanoparticles ◽  
Anticancer Agents ◽  
Sem Analysis ◽  
Dependent Manner ◽  
Anticancer Activities ◽  
Anticancer Efficacy ◽  
Hct 116 ◽  
Hct 116 Cells ◽  
Dose Dependent

The current study demonstrates the synthesis of fatty acids (FAs) capped silver nanoparticles (AgNPs) using aqueous poly-herbal drug Liv52 extract (PLE) as a reducing, dispersing and stabilizing agent. The NPs were characterized by various techniques and used to investigate their potent antibacterial, antibiofilm, antifungal and anticancer activities. GC-MS analysis of PLE shows a total of 37 peaks for a variety of bio-actives compounds. Amongst them, n-hexadecanoic acid (21.95%), linoleic acid (20.45%), oleic acid (18.01%) and stearic acid (13.99%) were found predominately and most likely acted as reducing, stabilizing and encapsulation FAs in LIV-AgNPs formation. FTIR analysis of LIV-AgNPs shows some other functional bio-actives like proteins, sugars and alkenes in the soft PLE corona. The zone of inhibition was 10.0 ± 2.2–18.5 ± 1.0 mm, 10.5 ± 2.5–22.5 ± 1.5 mm and 13.7 ± 1.0–16.5 ± 1.2 against P. aeruginosa, S. aureus and C. albicans, respectively. LIV-AgNPs inhibit biofilm formation in a dose-dependent manner i.e., 54.4 ± 3.1%—10.12 ± 2.3% (S. aureus), 72.7 ± 2.2%–23.3 ± 5.2% (P. aeruginosa) and 85.4 ± 3.3%–25.6 ± 2.2% (C. albicans), and SEM analysis of treated planktonic cells and their biofilm biomass validated the fitness of LIV-AgNPs in future nanoantibiotics. In addition, as prepared FAs rich PLE capped AgNPs have also exhibited significant (p < 0.05 *) antiproliferative activity against cultured HCT-116 cells. Overall, this is a very first demonstration on employment of FAs rich PLE for the synthesis of highly dispersible, stable and uniform sized AgNPs and their antibacterial, antifungal, antibiofilm and anticancer efficacy.

scholarly journals Single-Step Green Synthesis of Highly Concentrated and Stable Colloidal Dispersion of Core-Shell Silver Nanoparticles and Their Antimicrobial and Ultra-High Catalytic Properties

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1007
Author(s):  
Azam Ali ◽  
Mariyam Sattar ◽  
Fiaz Hussain ◽  
Muhammad Humble Khalid Tareen ◽  
Jiri Militky ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Tannic Acid ◽  
Rate Constant ◽  
Catalytic Properties ◽  
Colloidal Dispersion ◽  
Alkaline Condition ◽  
Core Shell ◽  
Average Particle ◽  
Ag Nps

The versatile one-pot green synthesis of a highly concentrated and stable colloidal dispersion of silver nanoparticles (Ag NPs) was carried out using the self-assembled tannic acid without using any other hazardous chemicals. Tannic acid (Plant-based polyphenol) was used as a reducing and stabilizing agent for silver nitrate in a mild alkaline condition. The synthesized Ag NPs were characterized for their concentration, capping, size distribution, and shape. The experimental results confirmed the successful synthesis of nearly spherical and highly concentrated (2281 ppm) Ag NPs, capped with poly-tannic acid (Ag NPs-PTA). The average particle size of Ag NPs-PTA was found to be 9.90 ± 1.60 nm. The colloidal dispersion of synthesized nanoparticles was observed to be stable for more than 15 months in the ambient environment (25 °C, 65% relative humidity). The synthesized AgNPs-PTA showed an effective antimicrobial activity against Staphylococcus Aureus (ZOI 3.0 mM) and Escherichia coli (ZOI 3.5 mM). Ag NPs-PTA also exhibited enhanced catalytic properties. It reduces 4-nitrophenol into 4-aminophenol in the presence of NaBH4 with a normalized rate constant (Knor = K/m) of 615.04 mL·s−1·mg−1. For comparison, bare Ag NPs show catalytic activity with a normalized rate constant of 139.78 mL·s−1·mg−1. Furthermore, AgNPs-PTA were stable for more than 15 months under ambient conditions. The ultra-high catalytic and good antimicrobial properties can be attributed to the fine size and good aqueous stability of Ag NPs-PTA. The unique core-shell structure and ease of synthesis render the synthesized nanoparticles superior to others, with potential for large-scale applications, especially in the field of catalysis and medical.

scholarly journals Synthesis, Characterization of Nano-β-Tricalcium Phosphate and the Inhibition on Hepatocellular Carcinoma Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Langlang Liu ◽  
Yanzeng Wu ◽  
Chao Xu ◽  
Suchun Yu ◽  
Xiaopei Wu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Particle Size ◽  
Cell Viability ◽  
Tricalcium Phosphate ◽  
Hepg2 Cells ◽  
Drug Carrier ◽  
Average Particle Size ◽  
Crystal Habit ◽  
Average Particle ◽  
Dependent Manner

It is difficult to synthesize nano-β-tricalcium phosphate (nano-β-TCP) owing to special crystal habit. The aim of this work was to synthesize nano-β-TCP using ethanol-water system and characterize it by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Malvern laser particle size analyzer, and transmission electron microscope (TEM). In addition, the inhibitory effect of nano-β-TCP on human hepatocellular carcinoma (HepG2) cells was also investigated using MTT assay, lactate dehydrogenase (LDH) leakage test, and 4′-6-diamidino-2-phenylindole (DAPI) staining. The results showed that negatively charged rod-like nano-β-TCP with about 55 nm in diameter and 120 nm in length was synthesized, and the average particle size of nano-β-TCP was 72.7 nm. The cell viability revealed that nano-β-TCP caused reduced cell viability of HepG2 cells in a time- and dose-dependent manner. These findings presented here may provide valuable reference data to guide the design of nano-β-TCP-based anticancer drug carrier and therapeutic systems in the future.

Study on the synthesis of antibacterial plastic by using silver nanoparticles doped in zeolite framework

2021 ◽  
Vol 6 (1) ◽  
pp. 32-36
Author(s):  
Anh Quoc Le ◽  
Van Phu Dang ◽  
Ngoc Duy Nguyen ◽  
Kim Lan Nguyen Thi ◽  
Kim Lang Vo Thi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Bactericidal Effect ◽  
Ethanol Solution ◽  
Average Particle ◽  
Γ Irradiation ◽  
Zeolite Framework ◽  
Ion Exchange Reaction ◽  
Transmission Electron

Silver nanoparticles (AgNPs) doped in the zeolite framework (AgNPs/Z) were successfully synthesized by γ-irradiation in ethanol solution of silver ion-zeolite (Ag+/Z) prepared by ion exchange reaction between silver nitrate (AgNO3) and zeolite 4A. The effects of the Ag+ concentration and irradiation dose on the formation of AgNPs/Z were also investigated. AgNPs/Z with the silver content of about 10,000 ppm and the average particle size of AgNPs of about 27 nm was characterized by ultraviolet-visible spectroscopy, powder X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM). Firstly, AgNPs/Z was added into PP resins for creation of PP-AgNPs/Z masterbatch (Ag content of ~10.000 ppm) and then PP-AgNPs/Z plastics were preapared by mixing masterbatch with PP resins. The antibacterial activity of the PP-AgNPs/Z plastics was investigated against Gram-negative bacteria Escherichia coli (E. coli). The results showed that PP-AgNPs/Z plastic contained 100 ppm of Ag possessed a high antibacterial property, namely the bactericidal effect was more than 96 % on the platic surface. In conclusion, possessing many advantages such as: vigorously antibacterial effect and good dispersion in plastic matrix, AgNPs/Z is promising to be applied as bactericidal agent for plastic industry.

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