scholarly journals CORP: Measurement of lung function in small animals

2017 ◽  
Vol 123 (5) ◽  
pp. 1039-1046 ◽  
Author(s):  
Jason H.T. Bates
Keyword(s):  
Lung Function ◽  
Impedance Measurement ◽  
Small Animal ◽  
Clinical Pathology ◽  
Transpulmonary Pressure ◽  
Forced Oscillation Technique ◽  
Small Animals ◽  
History Of ◽  
Series Of Experiments ◽  
Animal Handling

The measurement of lung function in mice and rats is crucial for understanding how well small animal models of pulmonary disease recapitulate human clinical pathology but brings with it the challenge of making accurate measurements in animals as small as a mouse. Overcoming these challenges can be achieved in a number of ways, each based on a model idealization of how the lung works as a mechanical system. Accordingly, it is important to understand the theoretical basis on which an assessment of lung function rests to interpret experimental measurements appropriately. It is also crucial to attend to a number of practical issues that determine the quality of the measurements. The most accurate measurements of lung function in small animals are provided by the forced oscillation technique that provides lung resistance and elastance and its multifrequency generalization known as impedance. Measurement quality is maximized when the greatest possible degree of control is exerted over the amplitude and frequency with which air is oscillated in and out of the lungs, the mean or end-expiratory transpulmonary pressure pertaining to when the oscillations are applied, and the immediate past volume history of the lungs. It is also crucial that no spontaneous breathing efforts occur during the measurement period. Finally, there is no substitute for the skill in animal handling and surgical preparation that comes with practice; such a skill should be in place before embarking on any important series of experiments.

scholarly journals Effect of Phalaris aquatica on the abundance and diversity of vertebrate animals in Mediterranean coastal grasslands in California

2017 ◽  
Author(s):  
Angela Joseph ◽  
Basma Nazal ◽  
Neha Saini ◽  
Farwa Sajadi ◽  
Ryan Ye
Keyword(s):  
Native Species ◽  
Vegetative State ◽  
Small Animal ◽  
Geographic Location ◽  
Small Animals ◽  
Tall Grass ◽  
Phalaris Aquatica ◽  
Animal Abundance ◽  
History Of ◽  
Abundance And Diversity

Harding grass (Phalaris aquatica), an invasive non-native species of bunchgrass, has been introduced to grasslands in many regions of California, particularly those with a history of disturbance, such as tilling and grazing. Due do the invasive nature of Harding grass, we sought to examine whether it has an effect on small animal abundance and diversity in the grasslands, Rancho Marino Reserve (RM) and Fiscalini Ranch Preserve (FR) in California. Both grasslands have similar climate and geographic location but differ in management history. Two transects were created in each site, with eight plots per transect. Animal cameras were deployed over the course of three nights to examine the abundance and diversity of small animals. Due to the history of tilling and planting of RM, and its increase in P. aquatica coverage, there was less animal abundance and diversity compared to FR. The results indicated that the untilled/unplanted areas had more animal abundance and diversity compared to tilled/planted due to the lack of Harding Grass. This can be due to factors such as diminished soil quality, difficulty in maneuvering in the tall grass, and adaptability to native vegetative state. Invasive plants have the ability to increase rapidly in space and potentially lead to ecosystem degradation. This adds further knowledge in the relationship between small animals and their habitats and helps conservation biologists ensure mammalian populations remain stable.

scholarly journals Effect of Phalaris aquatica on the abundance and diversity of vertebrate animals in Mediterranean coastal grasslands in California

2017 ◽  
Author(s):  
Angela Joseph ◽  
Basma Nazal ◽  
Neha Saini ◽  
Farwa Sajadi ◽  
Ryan Ye
Keyword(s):  
Native Species ◽  
Vegetative State ◽  
Small Animal ◽  
Geographic Location ◽  
Small Animals ◽  
Tall Grass ◽  
Phalaris Aquatica ◽  
Animal Abundance ◽  
History Of ◽  
Abundance And Diversity

Harding grass (Phalaris aquatica), an invasive non-native species of bunchgrass, has been introduced to grasslands in many regions of California, particularly those with a history of disturbance, such as tilling and grazing. Due do the invasive nature of Harding grass, we sought to examine whether it has an effect on small animal abundance and diversity in the grasslands, Rancho Marino Reserve (RM) and Fiscalini Ranch Preserve (FR) in California. Both grasslands have similar climate and geographic location but differ in management history. Two transects were created in each site, with eight plots per transect. Animal cameras were deployed over the course of three nights to examine the abundance and diversity of small animals. Due to the history of tilling and planting of RM, and its increase in P. aquatica coverage, there was less animal abundance and diversity compared to FR. The results indicated that the untilled/unplanted areas had more animal abundance and diversity compared to tilled/planted due to the lack of Harding Grass. This can be due to factors such as diminished soil quality, difficulty in maneuvering in the tall grass, and adaptability to native vegetative state. Invasive plants have the ability to increase rapidly in space and potentially lead to ecosystem degradation. This adds further knowledge in the relationship between small animals and their habitats and helps conservation biologists ensure mammalian populations remain stable.

Recommendations for Intensive Care Management in Small Animals Following Anaesthesia

1992 ◽  
Vol 05 (02) ◽  
pp. 66-70
Author(s):  
Karol Mathews ◽  
Doris Dyson
Keyword(s):  
Intensive Care ◽  
Care Management ◽  
Small Animal ◽  
Small Animals ◽  
Monitoring Equipment ◽  
Intensive Care Management ◽  
Good Communication

Intensive care management can be provided in a small animal facility by centralisation of emergency and monitoring equipment. Good communication between all personnel involved in the case ensures that staff are prepared for complications that could arise related to recovery from anaesthesia.

Thanato-Laboratorien. Theorien von Tod und Sterben und Elias Canettis Buch gegen den Tod

2020 ◽  
Vol 4 (1) ◽  
pp. 67-86
Author(s):  
Elisabeth Heyne
Keyword(s):  
Human Life ◽  
Death And Dying ◽  
Point Of View ◽  
Visual Images ◽  
Elias Canetti ◽  
History Of ◽  
Series Of Experiments ◽  
First Time ◽  
Textual Form ◽  
Or History

AbstractAlthough visual culture of the 21th century increasingly focuses on representation of death and dying, contemporary discourses still lack a language of death adequate to the event shown by pictures and visual images from an outside point of view. Following this observation, this article suggests a re-reading of 20th century author Elias Canetti. His lifelong notes have been edited and published posthumously for the first time in 2014. Thanks to this edition Canetti's short texts and aphorisms can be focused as a textual laboratory in which he tries to model a language of death on experimental practices of natural sciences. The miniature series of experiments address the problem of death, not representable in discourses of cultural studies, system theory or history of knowledge, and in doing so, Canetti creates liminal texts at the margins of western concepts of (human) life, science and established textual form.

scholarly journals History of IgA Nephropathy Mouse Models

2021 ◽  
Vol 10 (14) ◽  
pp. 3142
Author(s):  
Batoul Wehbi ◽  
Virginie Pascal ◽  
Lina Zawil ◽  
Michel Cogné ◽  
Jean-Claude Aldigier
Keyword(s):  
Iga Nephropathy ◽  
Small Animal ◽  
Experimental Models ◽  
Murine Models ◽  
Therapeutic Approaches ◽  
Complement Components ◽  
Simple Description ◽  
Primary Glomerulonephritis ◽  
History Of ◽  
Small Animal Models

IgA nephropathy (IgAN) is the most common primary glomerulonephritis in the world. It was first described in 1968 by Jean Berger and Nicole Hinglais as the presence of intercapillary deposits of IgA. Despite this simple description, patients with IgAN may present very broad clinical features ranging from the isolated presence of IgA in the mesangium without clinical or biological manifestations to rapidly progressive kidney failure. These features are associated with a variety of histological lesions, from the discrete thickening of the mesangial matrix to diffuse cell proliferation. Immunofluorescence on IgAN kidney specimens shows the isolated presence of IgA or its inconsistent association with IgG and complement components. This clinical heterogeneity of IgAN clearly echoes its complex and multifactorial pathophysiology in humans, inviting further analyses of its various aspects through the use of experimental models. Small-animal models of IgAN provide the most pertinent strategies for studying the multifactorial aspects of IgAN pathogenesis and progression. Although only primates have the IgA1 subclass, several murine models have been developed in which various aspects of immune responses are deregulated and which are useful in the understanding of IgAN physiopathology as well as in the assessment of IgAN therapeutic approaches. In this manuscript, we review all murine IgAN models developed since 1968 and discuss their remarkable contribution to understanding the disease.

scholarly journals A System-Agnostic, Adaptable and Extensible Animal Support Cradle System for Cardio-Respiratory-Synchronised, and Other, Multi-Modal Imaging of Small Animals

Tomography ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 39-54
Author(s):  
Veerle Kersemans ◽  
Stuart Gilchrist ◽  
Philip Danny Allen ◽  
Sheena Wallington ◽  
Paul Kinchesh ◽  
...  
Keyword(s):  
Scientific Data ◽  
Cardiac Monitoring ◽  
Small Animals ◽  
Maintenance System ◽  
Wide Range ◽  
Set Up ◽  
Animal Handling ◽  
Imaging Performance ◽  
Significant Effort

Standardisation of animal handling procedures for a wide range of preclinical imaging scanners will improve imaging performance and reproducibility of scientific data. Whilst there has been significant effort in defining how well scanners should operate and how in vivo experimentation should be practised, there is little detail on how to achieve optimal scanner performance with best practices in animal welfare. Here, we describe a system-agnostic, adaptable and extensible animal support cradle system for cardio-respiratory-synchronised, and other, multi-modal imaging of small animals. The animal support cradle can be adapted on a per application basis and features integrated tubing for anaesthetic and tracer delivery, an electrically driven rectal temperature maintenance system and respiratory and cardiac monitoring. Through a combination of careful material and device selection, we have described an approach that allows animals to be transferred whilst under general anaesthesia between any of the tomographic scanners we currently or have previously operated. The set-up is minimally invasive, cheap and easy to implement and for multi-modal, multi-vendor imaging of small animals.

scholarly journals Mucosal Challenge Ferret Models of Ebola Virus Disease

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 292
Author(s):  
Trevor Brasel ◽  
Jason E. Comer ◽  
Shane Massey ◽  
Jeanon Smith ◽  
Jennifer Smith ◽  
...  
Keyword(s):  
Disease Progression ◽  
Intranasal Administration ◽  
Ebola Virus ◽  
Advanced Disease ◽  
Virus Disease ◽  
Small Animal ◽  
Clinical Pathology ◽  
Small Animal Model ◽  
Post Challenge ◽  
Mustela Putorius

Recent studies have shown the domestic ferret (Mustela putorius furo) to be a promising small animal model for the study of Ebola virus (EBOV) disease and medical countermeasure evaluation. To date, most studies have focused on traditional challenge routes, predominantly intramuscular and intranasal administration. Here, we present results from a non-clinical pathogenicity study examining oronasal, oral, and ocular mucosal challenge routes in ferrets. Animals were challenged with 1, 10, or 100 plaque forming units EBOV followed by monitoring of disease progression and biosampling. Ferrets administered virus via oronasal and oral routes met euthanasia criteria due to advanced disease 5–10 days post-challenge. Conversely, all ferrets dosed via the ocular route survived until the scheduled study termination 28-day post-challenge. In animals that succumbed to disease, a dose/route response was not observed; increases in disease severity, febrile responses, serum and tissue viral load, alterations in clinical pathology, and gross/histopathology findings were similar between subjects. Disease progression in ferrets challenged via ocular administration was unremarkable throughout the study period. Results from this study further support the ferret as a model for EBOV disease following oral and nasal mucosa exposure.

scholarly journals Natural history of lung function over one year in patients with Parkinson's disease

2021 ◽  
Vol 182 ◽  
pp. 106396
Author(s):  
David A. Kaminsky ◽  
Donald G. Grosset ◽  
Deena M. Kegler-Ebo ◽  
Salvador Cangiamilla ◽  
Michael Klingler ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lung Function ◽  
Natural History ◽  
History Of ◽  
One Year

EMPTY BODY WEIGHTS, CARCASS WEIGHTS AND OFFAL PROPORTIONS IN BULLS AND STEERS OF DIFFERENT MATURE SIZE

1984 ◽  
Vol 64 (1) ◽  
pp. 53-57 ◽  
Author(s):  
S. D. M. JONES ◽  
R. E. ROMPALA ◽  
J. W. WILTON ◽  
C. H. WATSON
Keyword(s):  
Body Weight ◽  
Small Animal ◽  
High Energy ◽  
Carcass Weight ◽  
Small Animals ◽  
Beef Steers ◽  
Body Weights ◽  
Breed Crosses ◽  
Large Animals ◽  
Animal Size

Empty body weights, carcass weights and offal proportions were compared in 33 young beef bulls and 33 beef steers of different mature body size (35 small or mainly British breed crosses, 31 large or Continental crosses). All cattle were fed a high energy diet based on corn silage and high moisture corn from weaning to slaughter. Slaughter was carried out once 6 mm of fat had been attained at the 11/12th ribs, determined ultrasonically. Feed was removed 24 h and water 16 h prior to slaughter. The offal components were all weighed fresh and the alimentary components emptied of digesta. Bulls weighed 8.0% heavier (P < 0.05) than steers at slaughter, while large animals were 38.7% heavier (P < 0.0001) than small animals. Bulls and large animals had carcasses that dressed out 1.5% heavier than steers and small animals. To eliminate the effect of gutfill, carcass weights and offal components were expressed as a proportion of empty body weight. Bulls had a higher proportion of warm carcass weight and lower proportions of liver, spleen, heart, lungs, rumen, abomasum, large intestine and front feet relative to empty body weight than steers. Large animals had a greater proportion of warm carcass weight and hind feet, and a lower proportion of head, hide, liver, kidneys, omasum and small intestine relative to empty body weight than small animals. All castration by size interactions for liveweight, carcass weight, empty body weight and offal proportions were not significant. Castration and small animal size both increased the proportion of noncarcass parts relative to empty body weight in animals slaughtered at similar finish. Key words: Body, carcass, offal, bull, steer, maturity

Use of the Leksell Gamma Knife for Localized Small Field Lens Irradiation in Rodents

2003 ◽  
Vol 2 (5) ◽  
pp. 449-454 ◽  
Author(s):  
Colleen DesRosiers ◽  
Marc S. Mendonca ◽  
Craig Tyree ◽  
Vadim Moskvin ◽  
Morris Bank ◽  
...  
Keyword(s):  
Gamma Knife ◽  
High Precision ◽  
Small Animal ◽  
Small Animals ◽  
Small Field ◽  
Thermoluminescent Dosimeters ◽  
Ion Chamber ◽  
Order Of Magnitude ◽  
Contralateral Eye

For most basic radiobiological research applications involving irradiation of small animals, it is difficult to achieve the same high precision dose distribution realized with human radiotherapy. The precision for irradiations performed with standard radiotherapy equipment is ±2 mm in each dimension, and is adequate for most human treatment applications. For small animals such as rodents, whose organs and tissue structures may be an order of magnitude smaller than those of humans, the corresponding precision required is closer to ±0.2 mm, if comparisons or extrapolations are to be made to human data. The Leksell Gamma Knife is a high precision radiosurgery irradiator, with precision in each dimension not exceeding 0.5 mm, and overall precision of 0.7 mm. It has recently been utilized to treat ocular melanoma and induce targeted lesions in the brains of small animals. This paper describes the dosimetry and a technique for performing irradiation of a single rat eye and lens with the Gamma Knife while allowing the contralateral eye and lens of the same rat to serve as the “control”. The dosimetry was performed with a phantom in vitro utilizing a pinpoint ion chamber and thermoluminescent dosimeters, and verified by Monte Carlo simulations. We found that the contralateral eye received less than 5% of the administered dose for a 15 Gy exposure to the targeted eye. In addition, after 15 Gy irradiation 15 out of 16 animals developed cataracts in the irradiated target eyes, while 0 out of 16 contralateral eyes developed cataracts over a 6-month period of observation. Experiments at 5 and 10 Gy also confirmed the lack of cataractogenesis in the contralateral eye. Our results validate the use of the Gamma Knife for cataract studies in rodents, and confirmed the precision and utility of the instrument as a small animal irradiator for translational radiobiology experiments.

Sign in / Sign up

Export Citation Format

Share Document