Modulations by dietary restriction on antioxidant enzymes and lipid peroxidation in developing mice

2003 ◽  
Vol 94 (3) ◽  
pp. 947-952 ◽  
Author(s):  
Aiguo Wu ◽  
Xiufa Sun ◽  
Fada Wan ◽  
Yugu Liu
Keyword(s):  
Lipid Peroxidation ◽  
Food Intake ◽  
Dietary Restriction ◽  
Body Weight Gain ◽  
Antioxidant Systems ◽  
Sod Activity ◽  
Male And Female ◽  
Female Mice ◽  
Gpx Activity ◽  
Developing Mice

The effects of dietary restriction (DR) on the activities of liver superoxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GPX) and the level of lipid peroxidation (LP) in developing mice were investigated in this study. Male and female Kunmin mice were fed a standard rodent diet ad libitum (AL), 80% of AL food intake (20% DR), or 65% of AL food intake (35% DR) for 12 or 24 wk. Both 12 and 24 wk of DR resulted in retarded body weight gain in male and female mice. The activities of SOD, Cat, and GPX and the content of LP in DR male and female mice were not different ( P > 0.05) from those in controls after 12 wk of DR. However, the SOD activity was increased at 24 wk in 20% DR ( P < 0.05) and 35% DR ( P < 0.01) male, but not in DR female, mice. The Cat activity was elevated at 24 wk in both DR male ( P < 0.05 for 20% DR, P < 0.01 for 35% DR) and female ( P < 0.01) mice with a greater increase in DR female ( P < 0.05) than in DR male animals. GPX activity was also increased at 24 wk in DR male ( P < 0.01) and female ( P < 0.01) mice with a greater elevation in DR females ( P < 0.05) than in DR males. Furthermore, LP was decreased at 24 wk in both DR male ( P < 0.01) and female ( P < 0.01) animals with a greater reduction in DR females ( P< 0.01) compared with DR males. These findings indicated that 24 wk, but not 12 wk, of DR led to differential effects on liver SOD, Cat, and GPX activities and LP content in male and female mice during development, suggesting sex-associated modulations of DR on antioxidant systems in developing animals.

RAMP1 and RAMP3 Differentially Control Amylin’s Effects on Food Intake, Glucose and Energy Balance in Male and Female Mice

Neuroscience ◽  
2020 ◽  
Vol 447 ◽  
pp. 74-93 ◽  
Author(s):  
Bernd Coester ◽  
Sydney W. Pence ◽  
Soraya Arrigoni ◽  
Christina N. Boyle ◽  
Christelle Le Foll ◽  
...  
Keyword(s):  
Energy Balance ◽  
Food Intake ◽  
Male And Female ◽  
Female Mice

scholarly journals Poor Glycaemic Control Is Associated with Increased Lipid Peroxidation and Glutathione Peroxidase Activity in Type 2 Diabetes Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Harshi Prasadini Gunawardena ◽  
Renuka Silva ◽  
Ramiah Sivakanesan ◽  
Pathmasiri Ranasinghe ◽  
Prasad Katulanda
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Glycaemic Control ◽  
Study Groups ◽  
Cellular Damage ◽  
Lower Plasma ◽  
Sod Activity ◽  
Compensatory Response ◽  
Gpx Activity

Glycaemic control is the main focus of managing diabetes and its complications. Hyperglycaemia induces oxidative stress favouring cellular damage and subsequent diabetic complications. The present study was conducted to compare the plasma total antioxidant capacity (TAC) and individual antioxidant marker antioxidant status of type 2 diabetics (T2D) with good ((+) GC) and poor ((-) GC) glycaemic control with prediabetic (PDM) and normoglycaemic (NG) individuals. T2D (n=147), PDM (n=47), and NGC (n=106) were recruited as subjects. T2D and PDM had lower plasma TAG than NG subjects. T2D and PDM had significantly higher GPx activity and plasma MDA concentrations than NG. PDM showed the highest SOD activity. T2D (-) GC showed significantly elevated GPx activity and higher MDA level and significantly lower SOD activity among all study groups. Lower plasma TAC and higher plasma MDA indicate the presence of oxidative stress in T2D and PDM. Elevated GPx activity in T2D, PDM, and particularly in T2D (-) GC suggests a compensatory response to counteract excess lipid peroxidation in the hyperglycaemic state. Decline in SOD activity advocates the presence of glycation and excess lipid peroxidation in T2D.

scholarly journals Effect of chronic infusion of olanzapine and clozapine on food intake and body weight gain in male and female rats

Life Sciences ◽  
2007 ◽  
Vol 81 (12) ◽  
pp. 1024-1030 ◽  
Author(s):  
SuJean Choi ◽  
Briana DiSilvio ◽  
JayLynn Unangst ◽  
John D. Fernstrom
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Food Intake ◽  
Body Weight Gain ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Chronic Infusion

Influence of commonly used clinical antidotes on antioxidant systems in human hepatocyte culture intoxicated with α-amanitin

2010 ◽  
Vol 30 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Jan Magdalan ◽  
Aleksandra Piotrowska ◽  
Agnieszka Gomułkiewicz ◽  
Tomasz Sozański ◽  
Adam Szeląg ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Culture Medium ◽  
Antioxidant Properties ◽  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Antioxidant Systems ◽  
Effective Protection ◽  
Sod Activity ◽  
Severe Hepatotoxicity ◽  
Primary Mechanism

α-Amanitin (α-AMA) is the main toxin of Amanita phalloides and its subspecies (A. virosa and A. verna). The primary mechanism of α-AMA toxicity is associated with protein synthesis blocking in hepatocytes. Additionally, α-AMA exhibits prooxidant properties that may contribute to its severe hepatotoxicity. The aim of the present study was to assess the effect of α-AMA on lipid peroxidation and the activities of superoxide dismutase (SOD) and catalase (CAT) in human hepatocyte culture. The effects of benzylpenicillin (BPCN), N-acetyl-L-cysteine (ACC), and silibinin (SIL) on SOD and CAT activities and on lipid peroxidation in human hepatocyte culture intoxicated with α-AMA were also examined. In human hepatocyte culture, 48-hour exposure to α-AMA at a 2-μM concentration caused an increase in SOD activity, a reduction of CAT activity, and a significant increase in lipid peroxidation. Changes in SOD and CAT activity caused by α-AMA could probably enhance lipid peroxidation by increased generation of hydrogen peroxide combined with reduced detoxification of that oxygen radical. The addition of antidotes (ACC or SIL) to the culture medium provided more effective protection against lipid peroxidation in human hepatocytes intoxicated with α-AMA than the addition of BPCN, possessing no antioxidant properties.

scholarly journals A Reevaluation of the Effect of Dietary Restriction on Different Recombinant Inbred (RI) Lines of Male and Female Mice

2021 ◽  
Author(s):  
Archana Unnikrishnan ◽  
Stephanie Matyi ◽  
Karla Garrett Garrett ◽  
Michelle Ranjo-Bishop ◽  
David B Allison ◽  
...  
Keyword(s):  
Body Composition ◽  
Dietary Restriction ◽  
Recombinant Inbred ◽  
Research Community ◽  
Male And Female ◽  
Female Mice ◽  
Aging Mechanism ◽  
Median Lifespan ◽  
First Time ◽  
Ri Lines

Dietary restriction (DR) was reported to either have no effect or reduced the lifespan of the majority of the 41-recombinant inbred (RI)-lines studied (Liao et al., 2010). In an appropriately power longevity study (n > 30 mice/group), we measured the lifespan of the four RI-lines (115-RI, 97-RI, 98-RI, and 107-RI) that were reported to have the greatest decrease in lifespan when fed 40% DR. DR increased the median lifespan of female and male 115-RI mice and female 97-RI and 107-RI mice. DR had little effect (less than 4%) on the median lifespan of female and male 98-RI mice and male 97-RI mice and reduced the lifespan of male 107-RI mice over 20%. While our study was unable to replicate the effect of DR on the lifespan of the RI-mice (except male 107-RI mice) reported by Liao et al. (2010), we found that the genotype of a mouse had a major impact on the effect of DR on lifespan, with the effect of DR ranging from a 50% increase to a 22% decrease. No correlation was observed between the changes in either body composition or glucose tolerance induced by DR and the changes observed in lifespan of the four RI-lines of male and female mice. These four RI-lines of mice give the research community a unique resource where investigators for the first time can study the anti-aging mechanism of DR by comparing mice in which DR increases lifespan to mice where DR has either no effect or reduces lifespan.

scholarly journals TPH2 in the Dorsal Raphe Nuclei Regulates Energy Balance in a Sex-Dependent Manner

Endocrinology ◽  
2020 ◽  
Vol 162 (1) ◽  
Author(s):  
Hailan Liu ◽  
Chunmei Wang ◽  
Meng Yu ◽  
Yongjie Yang ◽  
Yang He ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Energy Balance ◽  
Food Intake ◽  
Dorsal Raphe ◽  
Raphe Nuclei ◽  
Male And Female ◽  
Female Mice ◽  
Raphé Nuclei ◽  
Dorsal Raphé

Abstract AbstractCentral 5-hydroxytryptamine (5-HT), which is primarily synthesized by tryptophan hydroxylase 2 (TPH2) in the dorsal Raphe nuclei (DRN), plays a pivotal role in the regulation of food intake and body weight. However, the physiological functions of TPH2 on energy balance have not been consistently demonstrated. Here we systematically investigated the effects of TPH2 on energy homeostasis in adult male and female mice. We found that the DRN harbors a similar amount of TPH2+ cells in control male and female mice. Adult-onset TPH2 deletion in the DRN promotes hyperphagia and body weight gain only in male mice, but not in female mice. Ablation of TPH2 reduces hypothalamic pro-opiomelanocortin (POMC) neuronal activity robustly in males, but only to a modest degree in females. Deprivation of estrogen by ovariectomy (OVX) causes comparable food intake and weight gain in female control and DRN-specific TPH2 knockout mice. Nevertheless, disruption of TPH2 blunts the anorexigenic effects of exogenous estradiol (E2) and abolishes E2-induced activation of POMC neurons in OVX female mice, indicating that TPH2 is indispensable for E2 to activate POMC neurons and to suppress appetite. Together, our study revealed that TPH2 in the DRN contributes to energy balance regulation in a sexually dimorphic manner.

Abstract 077: Role of Suppressor of Cytokines Signaling 3 (SOCS3) in POMC Neurons in Regulating Metabolic and Cardiovascular Functions in Dietary-Induced Obesity

Hypertension ◽  
2016 ◽  
Vol 68 (suppl_1) ◽  
Author(s):  
Zhen Wang ◽  
Jussara do Carmo ◽  
Alexandre da Silva ◽  
Nicola Aberdein ◽  
John Hall
Keyword(s):  
Food Intake ◽  
Glucose Tolerance ◽  
Body Weight Gain ◽  
Negative Regulator ◽  
Leptin Resistance ◽  
Cytokine Signaling ◽  
Male And Female ◽  
Air Jet ◽  
Cardiovascular Functions

Suppressor of cytokine signaling 3 (SOCS3), a negative regulator of leptin signaling, may contribute to the development of obesity-induced leptin resistance. Previously, we showed that activation of proopiomelanocortin (POMC) neurons mediates the chronic effects of leptin on blood pressure (BP) and glucose regulation. However, the role of SOCS3 in POMC neurons in regulating metabolic and cardiovascular functions in obesity is still unclear. To address this question, we used male and female mice with SOCS3 deleted only in POMC neurons (SOCS3 flox/flox -POMC/cre) and flox control (SOCS3 flox/flox ) mice. After weaning, mice were fed normal chow until 20 wks of age; then glucose tolerance tests (GTT) were performed and telemetry probes were implanted to measure mean arterial pressure (MAP) 24-hrs/day. We found that at 23 wks of age, both male and female SOCS3 flox/flox -POMC/cre mice weighed less than control mice (32±1 vs 37±2 g in male and 25±1 vs 27±1 g in female, n=9-11, p <0.05), but had similar daily food intake (3.5±0.2 g in male and 3.3±0.1 g in female) and MAP (114±1 vs 115±2 mmHg). Only male SOCS3 flox/flox -POMC/cre mice exhibited improved glucose tolerance (AUC: 1059±52 vs 1283±54 mg/dL x 120 min, n=7-10, p <0.05) compared to controls. From 23 wks, mice were switched to a high fat diet (45%, HFD) for 6 wks. After HFD feeding, both male and female SOCS3 flox/flox -POMC/cre mice had slightly reduced food intake (3.2±0.1 vs 3.5±0.2 g in male and 2.7±0.1 vs 3.0±0.2 g in female) and a trend toward lower body weight gain (10±1 vs 12±1 g in male and 4±1 vs 6±1 g in female), although the differences were not significant compared to controls. However, male and female SOCS3 flox/flox -POMC/cre mice fed a HFD had significantly greater MAP increase (7±1 vs 1±1 mmHg, n=13-16, p <0.05) and an enhanced BP response to acute air-jet stress (AUC: 109±9 vs 74±12 mmHg x 5min, n=8-13, p <0.05). After HFD, glucose tolerance was impaired in all groups of mice compared to the baseline at 20 wks, but there were no differences between SOCS3 flox/flox -POMC/cre and controls. These results suggest that deletion of SOCS3 in POMC neurons amplifies the BP response to a HFD and to acute stress, but has minimal effects on metabolic functions to HFD. (NHLBI PO1HL51971, NIGMS P20GM104357, AHA 14POST18160019)

scholarly journals Reevaluation of the effect of dietary restriction on different recombinant inbred lines of male and female mice

Aging Cell ◽  
2021 ◽  
Author(s):  
Archana Unnikrishnan ◽  
Stephanie Matyi ◽  
Karla Garrett ◽  
Michelle Ranjo‐Bishop ◽  
David B. Allison ◽  
...  
Keyword(s):  
Dietary Restriction ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Male And Female ◽  
Female Mice

Effects of not predictable alternative overcrowding and isolation on food intake and body weight gain in male and female rats at weaning or adulthood

Appetite ◽  
2010 ◽  
Vol 54 (3) ◽  
pp. 638
Author(s):  
A. Cárdenas ◽  
V.A. López-Espinoza ◽  
F. Díaz ◽  
A.G. Martínez ◽  
K. Franco-Paredes ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Food Intake ◽  
Body Weight Gain ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats

Lipid peroxidation and trace element status in diabetic ketotic patients: influence of insulin therapy

1993 ◽  
Vol 39 (5) ◽  
pp. 789-793 ◽  
Author(s):  
P Faure ◽  
P Corticelli ◽  
M J Richard ◽  
J Arnaud ◽  
C Coudray ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Reference Values ◽  
Plasma Concentrations ◽  
Plasma Lipid ◽  
Diabetic Patients ◽  
Sod Activity ◽  
Plasma Selenium ◽  
Beneficial Effects ◽  
Insulin Dependent Diabetic ◽  
Gpx Activity

Abstract Lipid peroxidation is known to accelerate aging and microvascular lesions in diabetic patients. We studied the acute influence of improved glycemic control on the concentrations of plasma lipid peroxidation intermediates [malondialdehydes (MDA), organic hydroperoxides (OHP)] in ketotic insulin-dependent diabetic patients, as well as the interplay of enzymes such as glutathione peroxidase (GPX) and CuZn superoxide dismutase (CuZn-SOD), and trace elements (Zn, Se, Cu) postulated to be involved in free radical protection. These plasma components were measured on the first day of hospitalization (T0) and when the patient had attained a stable glycemic state after continuous insulin treatment (T1). Plasma MDA and OHP concentrations were high at the beginning of the study but approached reference values after glycemic equilibration. Plasma zinc concentrations were significantly (P &lt; 0.05) decreased during the ketotic state, but also approached reference values with glycemic equilibration. Plasma selenium concentrations and GPX activity were relatively unchanged between T0 and T1. Erythrocyte GPX activity measured at T1 in six patients was below the reference values, whereas CuZn-SOD activity was not affected. Our results show that enhanced lipid peroxidation is associated with decreased zinc plasma concentrations in ketotic patients and underline the beneficial effects of continuous insulin infusion. The mechanisms involved are still unclear but may indicate the importance of extracellular zinc transfer secondary to glycemic disorders.

Sign in / Sign up

Export Citation Format

Share Document