Lipid peroxidation and trace element status in diabetic ketotic patients: influence of insulin therapy

1993 ◽  
Vol 39 (5) ◽  
pp. 789-793 ◽  
Author(s):  
P Faure ◽  
P Corticelli ◽  
M J Richard ◽  
J Arnaud ◽  
C Coudray ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Reference Values ◽  
Plasma Concentrations ◽  
Plasma Lipid ◽  
Diabetic Patients ◽  
Sod Activity ◽  
Plasma Selenium ◽  
Beneficial Effects ◽  
Insulin Dependent Diabetic ◽  
Gpx Activity

Abstract Lipid peroxidation is known to accelerate aging and microvascular lesions in diabetic patients. We studied the acute influence of improved glycemic control on the concentrations of plasma lipid peroxidation intermediates [malondialdehydes (MDA), organic hydroperoxides (OHP)] in ketotic insulin-dependent diabetic patients, as well as the interplay of enzymes such as glutathione peroxidase (GPX) and CuZn superoxide dismutase (CuZn-SOD), and trace elements (Zn, Se, Cu) postulated to be involved in free radical protection. These plasma components were measured on the first day of hospitalization (T0) and when the patient had attained a stable glycemic state after continuous insulin treatment (T1). Plasma MDA and OHP concentrations were high at the beginning of the study but approached reference values after glycemic equilibration. Plasma zinc concentrations were significantly (P < 0.05) decreased during the ketotic state, but also approached reference values with glycemic equilibration. Plasma selenium concentrations and GPX activity were relatively unchanged between T0 and T1. Erythrocyte GPX activity measured at T1 in six patients was below the reference values, whereas CuZn-SOD activity was not affected. Our results show that enhanced lipid peroxidation is associated with decreased zinc plasma concentrations in ketotic patients and underline the beneficial effects of continuous insulin infusion. The mechanisms involved are still unclear but may indicate the importance of extracellular zinc transfer secondary to glycemic disorders.

scholarly journals Electronic and Tobacco Cigarettes Alter Polyunsaturated Fatty Acids and Oxidative Biomarkers

2021 ◽  
Author(s):  
Rajat Gupta ◽  
Yan Lin ◽  
Karla Luna ◽  
Anjali Logue ◽  
Alexander J Yoon ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Cardiovascular Risk ◽  
Polyunsaturated Fatty Acids ◽  
Low Density Lipoprotein ◽  
Plasma Concentrations ◽  
Multivariable Analysis ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Heme Oxygenase 1

Rationale: Chronic electronic cigarette (EC) users exhibit a higher susceptibility of low-density lipoprotein (LDL) to undergo oxidation as compared to non-user controls. However, there is a paucity of data regarding EC effects on lipid peroxidation in the blood and their relationship to cardiovascular risk. Objective: To test the hypothesis that chronic (≥1 year) EC use exerts intermediate effects on plasma lipid peroxidation and/or antioxidant defense compared to chronic tobacco cigarette (TC) smoking. Methods and Results: We enrolled EC-users (n=32), TC-smokers (n=29) and non-users (n=45), with mean ages of 28.3, 27.8 and 27.4 years, respectively. Plasma concentrations of free polyunsaturated fatty acids and oxidized metabolites were assessed by mass spectrometry. Total antioxidant capacity (TAC), concentrations of glutathione, bilirubin, heme oxygenase-1 (HO-1), and functional activity of paraoxonase1 (PON1) were determined by colorimetric and enzymatic assays. Multivariable analysis was performed using classification models for segregating participants based on biomarker profiles. Plasma arachidonic acid (AA) concentration was higher in TC-smokers but lower in EC-users, together with linoleic acid (LA) concentration, as compared to TC-smokers and non-users (p<0.05). Oxidized LA metabolites (9- and 13-hydroxyoctadecadienoic acid (HODE)) were lower in EC-users and TC-smokers as compared to non-users (p<0.001). Consistently, TAC and bilirubin were elevated in EC-users and TC-smokers as compared to non-users (p<0.05). Of interest, plasma HO-1 concentration was higher in TC-smokers as compared to non-users (p=0.01) with intermediate levels in EC-users. Multivariable analysis identified 5 biomarkers (13-HODE, LA, 9-HODE, 12-hydroxyeicosatetraenoic acid (HETE), AA) that discriminated EC-users from TC-smokers and non-users with an accuracy of 73.4%. Conclusions: Chronic use of EC induces common (i.e. lower 9- and/or 13-HODEs and higher TAC and bilirubin) as well as differential effects (i.e. altered AA and LA concentrations) to those induced by TC, along with intermediate plasma HO-1 concentration, suggesting that EC, likewise TC smoke, could impact cardiovascular risk.

scholarly journals Poor Glycaemic Control Is Associated with Increased Lipid Peroxidation and Glutathione Peroxidase Activity in Type 2 Diabetes Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Harshi Prasadini Gunawardena ◽  
Renuka Silva ◽  
Ramiah Sivakanesan ◽  
Pathmasiri Ranasinghe ◽  
Prasad Katulanda
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Glycaemic Control ◽  
Study Groups ◽  
Cellular Damage ◽  
Lower Plasma ◽  
Sod Activity ◽  
Compensatory Response ◽  
Gpx Activity

Glycaemic control is the main focus of managing diabetes and its complications. Hyperglycaemia induces oxidative stress favouring cellular damage and subsequent diabetic complications. The present study was conducted to compare the plasma total antioxidant capacity (TAC) and individual antioxidant marker antioxidant status of type 2 diabetics (T2D) with good ((+) GC) and poor ((-) GC) glycaemic control with prediabetic (PDM) and normoglycaemic (NG) individuals. T2D (n=147), PDM (n=47), and NGC (n=106) were recruited as subjects. T2D and PDM had lower plasma TAG than NG subjects. T2D and PDM had significantly higher GPx activity and plasma MDA concentrations than NG. PDM showed the highest SOD activity. T2D (-) GC showed significantly elevated GPx activity and higher MDA level and significantly lower SOD activity among all study groups. Lower plasma TAC and higher plasma MDA indicate the presence of oxidative stress in T2D and PDM. Elevated GPx activity in T2D, PDM, and particularly in T2D (-) GC suggests a compensatory response to counteract excess lipid peroxidation in the hyperglycaemic state. Decline in SOD activity advocates the presence of glycation and excess lipid peroxidation in T2D.

Effect of hyperketonemia on plasma lipid peroxidation levels in diabetic patients

Diabetes Care ◽  
1999 ◽  
Vol 22 (7) ◽  
pp. 1171-1175 ◽  
Author(s):  
S. K. Jain ◽  
R. McVie ◽  
R. Jackson ◽  
S. N. Levine ◽  
G. Lim
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Lipid ◽  
Diabetic Patients ◽  
Plasma Lipid Peroxidation

Modulations by dietary restriction on antioxidant enzymes and lipid peroxidation in developing mice

2003 ◽  
Vol 94 (3) ◽  
pp. 947-952 ◽  
Author(s):  
Aiguo Wu ◽  
Xiufa Sun ◽  
Fada Wan ◽  
Yugu Liu
Keyword(s):  
Lipid Peroxidation ◽  
Food Intake ◽  
Dietary Restriction ◽  
Body Weight Gain ◽  
Antioxidant Systems ◽  
Sod Activity ◽  
Male And Female ◽  
Female Mice ◽  
Gpx Activity ◽  
Developing Mice

The effects of dietary restriction (DR) on the activities of liver superoxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GPX) and the level of lipid peroxidation (LP) in developing mice were investigated in this study. Male and female Kunmin mice were fed a standard rodent diet ad libitum (AL), 80% of AL food intake (20% DR), or 65% of AL food intake (35% DR) for 12 or 24 wk. Both 12 and 24 wk of DR resulted in retarded body weight gain in male and female mice. The activities of SOD, Cat, and GPX and the content of LP in DR male and female mice were not different ( P > 0.05) from those in controls after 12 wk of DR. However, the SOD activity was increased at 24 wk in 20% DR ( P < 0.05) and 35% DR ( P < 0.01) male, but not in DR female, mice. The Cat activity was elevated at 24 wk in both DR male ( P < 0.05 for 20% DR, P < 0.01 for 35% DR) and female ( P < 0.01) mice with a greater increase in DR female ( P < 0.05) than in DR male animals. GPX activity was also increased at 24 wk in DR male ( P < 0.01) and female ( P < 0.01) mice with a greater elevation in DR females ( P < 0.05) than in DR males. Furthermore, LP was decreased at 24 wk in both DR male ( P < 0.01) and female ( P < 0.01) animals with a greater reduction in DR females ( P< 0.01) compared with DR males. These findings indicated that 24 wk, but not 12 wk, of DR led to differential effects on liver SOD, Cat, and GPX activities and LP content in male and female mice during development, suggesting sex-associated modulations of DR on antioxidant systems in developing animals.

scholarly journals Interplay between glycaemic status, lipid peroxidation and hyperlipidaemia in subjects with varying glucose metabolism

2019 ◽  
pp. 13
Author(s):  
Remva Paul ◽  
J.K. Mukkadan
Keyword(s):  
Lipid Peroxidation ◽  
Glucose Metabolism ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Total Cholesterol Level ◽  
Pearson Correlation ◽  
Density Lipoprotein ◽  
Diabetic Patients ◽  
Sod Activity ◽  
Cross Sectional

Background: Diabetes is one of the largest global health emergencies of the 21st century. About 425 million people worldwide or 8.8% of adults between the ages of 20-79 years are estimated to have diabetes. There are evidences which show that higher blood glucose and cholesterol levels have an association with free radical-mediated lipid peroxidation. The aim of the study was to compare and correlate glycosylated haemoglobin (HbA1c), lipid peroxidation marker malondialdehyde (MDA), lipid profile, and antioxidant enzyme superoxide dismutase (SOD) activity in subjects with varying levels of glucose metabolism. Materials and methods: This was a cross sectional study. Based on fasting plasma glucose and HbA1c, subjects were divided into diabetic patients (n= 40), pre-diabetic patients (n=38) and normal patients (n=41). Subjects were tested for lipid profile, MDA, and SOD activity. The comparison of parameter between the groups was carried out using one-way ANOVA followed by tukey’s multiple comparison test. The correlation between parameter was analyzed by Karl Pearson correlation coefficient using SPSS 20.0. Results: The serum MDA levels (nmol/ml) were significantly higher in pre-diabetes (3.11±0.40) and diabetes mellitus (3.55±0.88) compared to normal controls (2.04±0.99). The SOD activity(U/ml) were lower in diabetes (7.69±3.83) and pre-diabetic subjects (8.13±2.15) compared to normal subjects (11.16±3.61). MDA has significant positive correlation with HbA1c and total cholesterol level. SOD has significant negative correlation with HbA1c and Total cholesterol/High Density Lipoprotein (TC/ HDL) ratio. Conclusion: The findings strongly confirmed that there is a continuous interplay between glycemic status, lipid peroxidation and hyperlipidemia in which one factor perpetuates another leading to the progression of disease.

scholarly journals Characterization of Blood Oxidative Stress in Type 2 Diabetes Mellitus Patients: Increase in Lipid Peroxidation and SOD Activity

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Suziy de M. Bandeira ◽  
Glaucevane da S. Guedes ◽  
Lucas José S. da Fonseca ◽  
André S. Pires ◽  
Daniel P. Gelain ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Lipid Peroxidation ◽  
Diabetic Patients ◽  
Sod Activity ◽  
Glucose Levels ◽  
Significant Difference ◽  
Close Relationship

This study evaluated the oxidative stress through enzymatic and nonenzymatic biomarkers in diabetic patients with and without hypertension and prediabetics. The SOD and CAT (in erythrocytes) and GPx (in plasma) enzymatic activities, plasma levels of lipid peroxidation, and total thiols were measured in the blood of 55 subjects with type 2 diabetes and 38 subjects without diabetes (9 pre-diabetics and 29 controls) aged 40–86 years. The total SOD activity and the lipid peroxidation were higher in diabetics compared to nondiabetics. In stratified groups, the total SOD activity was different for the hypertensive diabetics compared to the prediabetics and normotensive controls. Lipid peroxidation was significantly higher in both groups of diabetics (hypertensive and normotensive) compared to prediabetic groups and hypertensive and normotensive controls. There was no significant difference in the CAT and GPx activities, as well as in the concentration of total thiols in the groups studied. Present data strongly suggest the involvement of oxidative stress in the pathophysiology of diabetes, revealing that the increased lipid peroxidation has a close relationship with high glucose levels, as observed by the fasting glucose and HbA1c levels. The results evidence the correlation between lipid peroxidation and DM, irrespective of the presence of hypertension.

scholarly journals Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Harikesh Maurya ◽  
Vaishali Mangal ◽  
Sanjay Gandhi ◽  
Kathiresan Prabhu ◽  
Kathiresan Ponnudurai
Keyword(s):  
Lipid Peroxidation ◽  
Gallic Acid ◽  
Large Population ◽  
Cecal Ligation ◽  
Sepsis Group ◽  
Acid Pretreatment ◽  
Sod Activity ◽  
Group Iii ◽  
Beneficial Effects ◽  
Group I

Present study is to investigate the effect of Gallic acid pretreatment on survival of septic animals and oxidative stress in different organs like lungs, liver, kidney, spleen, and vascular dysfunction of mice. Sepsis was induced by cecal ligation and puncture (CLP) in healthy adult male albino mice (25–30 g) and was divided into 3 groups each consisting of 6 animals, that is, sham-operated (SO group (Group I), SO + sepsis (Group II), and Gallic acid + sepsis (Group III)). Group III animals were pretreated with Gallic acid at the dose rate of 20 mg/kg body weight for 2 days before induction of sepsis. Animals were sacrificed on 8th day and the tissue samples were obtained for further investigation on lipid peroxidation (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH). Gallic acid pretreatment significant (P<0.05) reduces kidney, spleen, liver, and lungs’ malondialdehyde level in septic mice. However, it fails to improve reduced glutathione level in all given organs, while, Gallic acid pretreated mice showed significant improvement in SOD activity of kidney and spleen when compared to septic mice. Finally, the beneficial effects of Gallic acid pretreatment in sepsis are evident from the observations that Gallic acid partially restored SOD and catalase activity and completely reversed lipid peroxidation. Further studies are required to find out the possible mechanisms underlying the beneficial effects of Gallic acid on large population.

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