Microparticle production, neutrophil activation, and intravascular bubbles following open-water SCUBA diving

2012 ◽  
Vol 112 (8) ◽  
pp. 1268-1278 ◽  
Author(s):  
Stephen R. Thom ◽  
Tatyana N. Milovanova ◽  
Marina Bogush ◽  
Veena M. Bhopale ◽  
Ming Yang ◽  
...  
Keyword(s):  
Sea Water ◽  
Bubble Formation ◽  
Annexin V ◽  
Scuba Diving ◽  
Open Water ◽  
Surface Expression ◽  
Neutrophil Activation ◽  
Positive Correlations ◽  
The Impact ◽  
Neutrophil Surface

The goal of this study was to evaluate annexin V-positive microparticles (MPs) and neutrophil activation in humans following decompression from open-water SCUBA diving with the hypothesis that changes are related to intravascular bubble formation. Sixteen male volunteer divers followed a uniform profile of four daily SCUBA dives to 18 m of sea water for 47 min. Blood was obtained prior to and at 80 min following the first and fourth dives to evaluate the impact of repetitive diving, and intravascular bubbles were quantified by trans-thoracic echocardiography carried out at 20-min intervals for 2 h after each dive. MPs increased by 3.4-fold after each dive, neutrophil activation occurred as assessed by surface expression of myeloperoxidase and the CD18 component of β2-integrins, and there was an increased presence of the platelet-derived CD41 protein on the neutrophil surface indicating interactions with platelet membranes. Intravascular bubbles were detected in all divers. Surprisingly, significant inverse correlations were found among postdiving bubble scores and MPs, most consistently at 80 min or more after the dive on the fourth day. There were significant positive correlations between MPs and platelet-neutrophil interactions after the first dive and between platelet-neutrophil interactions and neutrophil activation documented as an elevation in β2-integrin expression after the fourth dive. We conclude that MPs- and neutrophil-related events in humans are consistent with findings in an animal decompression model. Whether there are causal relationships among bubbles, MPs, platelet-neutrophil interactions, and neutrophil activation remains obscure and requires additional study.

Bubbles, microparticles, and neutrophil activation: changes with exercise level and breathing gas during open-water SCUBA diving

2013 ◽  
Vol 114 (10) ◽  
pp. 1396-1405 ◽  
Author(s):  
Stephen R. Thom ◽  
Tatyana N. Milovanova ◽  
Marina Bogush ◽  
Ming Yang ◽  
Veena M. Bhopale ◽  
...  
Keyword(s):  
Transthoracic Echocardiography ◽  
Sea Water ◽  
Bubble Formation ◽  
Correlation Coefficients ◽  
Annexin V ◽  
Scuba Diving ◽  
Open Water ◽  
Neutrophil Activation ◽  
Dive Profile

The study goal was to evaluate responses in humans following decompression from open-water SCUBA diving with the hypothesis that exertion underwater and use of a breathing mixture containing more oxygen and less nitrogen (enriched air nitrox) would alter annexin V-positive microparticle (MP) production and size changes and neutrophil activation, as well as their relationships to intravascular bubble formation. Twenty-four divers followed a uniform dive profile to 18 m of sea water breathing air or 22.5 m breathing 32% oxygen/68% nitrogen for 47 min, either swimming with moderately heavy exertion underwater or remaining stationary at depth. Blood was obtained pre- and at 15 and 120 min postdive. Intravascular bubbles were quantified by transthoracic echocardiography postdive at 20-min intervals for 2 h. There were no significant differences in maximum bubble scores among the dives. MP number increased 2.7-fold, on average, within 15 min after each dive; only the air-exertion dive resulted in a significant further increase to 5-fold over baseline at 2 h postdive. Neutrophil activation occurred after all dives. For the enriched air nitrox stationary at depth dive, but not for other conditions, the numbers of postdive annexin V-positive particles above 1 μm in diameter were correlated with intravascular bubble scores (correlation coefficients ∼0.9, P < 0.05). We conclude that postdecompression relationships among bubbles, MPs, platelet-neutrophil interactions, and neutrophil activation appear to exist, but more study is required to improve confidence in the associations.

Ascorbic acid supplementation diminishes microparticle elevations and neutrophil activation following SCUBA diving

2015 ◽  
Vol 309 (4) ◽  
pp. R338-R344 ◽  
Author(s):  
Ming Yang ◽  
Otto F. Barak ◽  
Zeljko Dujic ◽  
Dennis Madden ◽  
Veena M. Bhopale ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Sea Water ◽  
Annexin V ◽  
Scuba Diving ◽  
Open Water ◽  
Face Mask ◽  
Neutrophil Activation ◽  
Acid Supplementation ◽  
Male Subjects ◽  
Ascorbic Acid Supplementation

Predicated on evidence that diving-related microparticle generation is an oxidative stress response, this study investigated the role that oxygen plays in augmenting production of annexin V-positive microparticles associated with open-water SCUBA diving and whether elevations can be abrogated by ascorbic acid. Following a cross-over study design, 14 male subjects ingested placebo and 2–3 wk later ascorbic acid (2 g) daily for 6 days prior to performing either a 47-min dive to 18 m of sea water while breathing air (∼222 kPa N2/59 kPa O2) or breathing a mixture of 60% O2/balance N2 from a tight-fitting face mask at atmospheric pressure for 47 min (∼40 kPa N2/59 kPa O2). Within 30 min after the 18-m dive in the placebo group, neutrophil activation, and platelet-neutrophil interactions occurred, and the total number of microparticles, as well as subgroups bearing CD66b, CD41, CD31, CD142 proteins or nitrotyrosine, increased approximately twofold. No significant elevations occurred among divers after ingesting ascorbic acid, nor were elevations identified in either group after breathing 60% O2. Ascorbic acid had no significant effect on post-dive intravascular bubble production quantified by transthoracic echocardiography. We conclude that high-pressure nitrogen plays a key role in neutrophil and microparticle-associated changes with diving and that responses can be abrogated by dietary ascorbic acid supplementation.

Beneficial effect of enriched air nitrox on bubble formation during scuba diving. An open-water study

2017 ◽  
Vol 36 (6) ◽  
pp. 605-612 ◽  
Author(s):  
Anne-Kathrin Brebeck ◽  
Andreas Deussen ◽  
Ursula Range ◽  
Costantino Balestra ◽  
Sinclair Cleveland ◽  
...  
Keyword(s):  
Beneficial Effect ◽  
Bubble Formation ◽  
Scuba Diving ◽  
Open Water

ASSOCIATION OF PLATELET-DERIVED MICROVESICLES WITH HIGH ON-TREATMENT PLATELET REACTIVITY IN CONVALESCENT ISCHEMIC STROKE PATIENTS TREATED WITH ACETYLSALICYLIC ACID

2019 ◽  
Vol 72 (8) ◽  
pp. 1426-1436
Author(s):  
Justyna Rosińska ◽  
Joanna Maciejewska ◽  
Robert Narożny ◽  
Wojciech Kozubski ◽  
Maria Łukasik
Keyword(s):  
Platelet Aggregation ◽  
Treatment Failure ◽  
Acetylsalicylic Acid ◽  
Platelet Reactivity ◽  
Study Groups ◽  
Surface Expression ◽  
Cerebrovascular Diseases ◽  
Stroke Patients ◽  
Vascular Events ◽  
The Impact

Introduction: Elevated concentrations of platelet-derived microvesicles are found in cerebrovascular diseases. The impact of acetylsalicylic acid on these microvesicles remains inconsistent, despite its well-established effect on platelet aggregation. High residual platelet aggregation is defined as high on-treatment platelet reactivity, while “treatment failure” is the occurrence of vascular events despite antiplatelet treatment. The aim of this study was to determine whether the antiaggregatory effect of acetylsalicylic acid correlates with platelet-derived microvesicles in convalescent ischaemic stroke patients and cardiovascular risk factor controls as well as to evaluate the association between high on-treatment platelet reactivity and recurrent vascular events with the studied platelet-derived microvesicle parameters. Materials and methods: The study groups consisted of 76 convalescent stroke patients and 74 controls. Total platelet-derived microvesicles, annexino-positive microvesicles number, and platelet-derived microvesicles with surface expression of proinflammatory (CD40L, CD62P, CD31) and procoagulant (PS, GPIIb/IIIa) markers were characterized and quantified using flow cytometry. Cyclooxygenase-1-specific platelet responsiveness, with whole blood impedance platelet aggregation under arachidonic acid stimulation and the serum concentration of thromboxane B2, were evaluated. Results: Neither acetylsalicylic acid intake nor modification of its daily dose caused statistically significant differences in the studied microvesicle parameters. Additionally, no statistically significant differences in the studied microvesicle parameters were revealed between high on-treatment platelet reactivity and non-high on-treatment platelet reactivity subjects in either study subgroup. However, elevated concentrations of PAC-1+/CD61+, CD62P+/CD61+ and CD31+/CD61+ microvesicles were found in stroke patients with treatment failure, defined in this study as a recurrent vascular events in a one-year follow-up period. Conclusions: This study revealed no relationship between circulating microvesicle number and platelet aggregation. The procoagulant and proinflammatory phenotype of circulating platelet-derived microvesicles might contribute to acetylsalicylic acid treatment failure.

Effect of long-term moisture exposure on impact response of glass-reinforced vinylester

2021 ◽  
pp. 147509022199616
Author(s):  
Fatemeh Alizadeh ◽  
Navid Kharghani ◽  
Carlos Guedes Soares
Keyword(s):  
Water Uptake ◽  
Failure Modes ◽  
Sea Water ◽  
Composite Plates ◽  
Impact Response ◽  
Low Velocity Impact ◽  
Water Type ◽  
Interfacial Damage ◽  
Impact Properties ◽  
The Impact

Glass/Vinylester composite laminates are comprehensively characterised to assess its impact response behaviour under moisture exposure in marine structures. An instrumented drop weight impact machine is utilised to determine the impact responses of dry and immersed specimens in normal, salted and sea water. The specimens, which had three different thicknesses, were subjected to water exposure for a very long period of over 20 months before tested in a low-velocity impact experiment. Water uptake was measured primarily to study the degradation profiles of GRP laminates after being permeated by water. Matrix dissolution and interfacial damage observed on the laminates after prolonged moisture exposure while the absorption behaviour was found typically non-Fickian. The weight of the composite plates firstly increased because of water diffusion up to month 15 and then decreased due to matrix degradation. The specimens with 3, 6 and 9 mm thickness exhibited maximum water absorption corresponding to 2.6%, 0.7% and 0.5% weight gain, respectively. In general, the results indicated that water uptake and impact properties were affected by thickness and less by water type. Impact properties of prolonged immersed specimens reduced remarkably, and intense failure modes detected almost in all cases. The least sensitive to impact damage were wet specimens with 9 mm thickness as they indicated similar maximum load and absorbed energy for different impact energies.

Microstructure and Corrosion Resistance of HVOF Sprayed 316L Stainless Steel and Ni Base Alloy Coatings

2000 ◽  
Author(s):  
S. Kuroda ◽  
T. Fukushima ◽  
T. Kodama ◽  
M. Sasaki
Keyword(s):  
Stainless Steel ◽  
Corrosion Resistance ◽  
316L Stainless Steel ◽  
Sea Water ◽  
Base Alloy ◽  
Standard Condition ◽  
Polarization Measurement ◽  
Superior Corrosion Resistance ◽  
Distribution Composition ◽  
The Impact

Abstract 316L stainless steel and Hastelloy C alloy powders were sprayed by an HVOF apparatus onto mild steel substrates. The microstructure, pore size distribution, composition and corrosion resistance of thus obtained coatings were evaluated experimentally. Corrosion resistance in sea-water was examined by monitoring the impedance and corrosion potential of samples immersed in artificial sea-water at 300 K over a period of more than 3 months and also by polarization measurement. It was found that the stainless coatings composed mainly of plastically deformed particles and some splats which were molten at the impact. By increasing the combustion pressure, the porosity as measured by mercury porosimeter could be reduced to below 1%. In comparison, Hastelloy C deposits sprayed under the standard condition were so dense that its porosity could not be measured by the porosimeter. The polarization curve and the results of impedance monitoring both exemplified that the Hastelloy C coatings possess much superior corrosion resistance to the stainless coatings in sea-water, which was attributed to the higher density and better adhesion of the Ni-base alloy coatings.

Bioresilience to Mercury Chloride of the Brine Shrimp Artemia Salina after Treatment with Homeopathic Mercurius Corrosivus

Homeopathy ◽  
2021 ◽  
Author(s):  
Andreia Adelaide G. Pinto ◽  
Mirian Y. de Oliveira Nagai ◽  
Ednar Nascimento Coimbra ◽  
Suham Nowrooz Mohammad ◽  
Jefferson Souza Silva ◽  
...  
Keyword(s):  
Sea Water ◽  
Specific Interaction ◽  
Artemia Salina ◽  
Mercury Content ◽  
Mercury Chloride ◽  
Hatching Rate ◽  
Toxic Substances ◽  
Protective Effects ◽  
Cyst Hatching ◽  
The Impact

Abstract Introduction Finding solutions to mitigate the impact of pollution on living systems is a matter of great interest. Homeopathic preparations of toxic substances have been described in the literature as attenuation factors for intoxication. Herein, an experimental study using Artemia salina and mercury chloride was developed as a model to identify aspects related to bioresilience. Aims The aim of the study was to describe the effects of homeopathic Mercurius corrosivus (MC) on Artemia salina cysts hatching and on mercury bioavailability. Methods Artemia salina cysts were exposed to 5.0 µg/mL of mercury chloride during the hatching phase. MC potencies (6cH, 30cH, and 200cH) were prepared in sterile purified water and poured into artificial sea water. Different controls were used (non-challenged cysts and challenged cysts treated with water, succussed water, and Ethilicum 1cH). Four series of nine experiments were performed to evaluate the percentage of cyst hatching. Soluble total mercury (THg) levels and precipitated mercury content were also evaluated. Solvatochromic dyes were used to check for eventual physicochemical markers of MC biological activity. Results Significant delay (p < 0.0001) in cyst hatching was observed only after treatment with MC 30cH, compared with controls. This result was associated with an increase of THg concentration in water (p = 0.0018) and of chlorine/oxygen ratio (p < 0.0001) in suspended micraggregates, suggesting changes in mercury bioavailability. A specific interaction of MC 30cH with the solvatochromic dye ET33 (p = 0.0017) was found. Conclusion Changes in hatching rate and possible changes in Hg bioavailability are postulated as protective effects of MC 30cH on Artemia salina, by improving its natural bioresilience processes.

Bubble Formation From Porous Plates in Liquid Cross-Flow

2018 ◽  
Author(s):  
Thomas Shepard ◽  
Eric Ruud ◽  
Henry Kinane ◽  
Deify Law ◽  
Kohl Ordahl
Keyword(s):  
Size Distribution ◽  
Bubble Size ◽  
Bubble Diameter ◽  
Bubble Formation ◽  
Cross Flow ◽  
Rectangular Channels ◽  
Gas Liquid Flow ◽  
Liquid Flows ◽  
Processing Techniques ◽  
The Impact

Controlling bubble diameter and bubble size distribution is important for a variety of applications and active fields of research. In this study the formation of bubbles from porous plates in a liquid cross-flow is examined experimentally. By injecting air through porous plates of various media grades (0.2 to 100) into liquid flows in rectangular channels of varying aspect ratio (1–10) and gas/liquid flow rates the impact of the various factors is presented. Image processing techniques were used to measure bubble diameters and capture their formation from the porous plates. Mean bubble diameters ranged from 0.06–1.21 mm. The present work expands upon the work of [1] and further identifies the relative importance of wall shear stress, air injector pore size and gas to liquid mass flow ratio on bubble size and size distribution.

scholarly journals Small waterbodies reduce the carbon sink of a polygonal tundra landscape

2021 ◽  
Author(s):  
Lutz Beckebanze ◽  
Zoé Rehder ◽  
David Holl ◽  
Charlotta Mirbach ◽  
Christian Wille ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbon Budget ◽  
Carbon Sink ◽  
Open Water ◽  
Methane Emissions ◽  
The Arctic ◽  
Sink Strength ◽  
Small Waterbodies ◽  
Methane Fluxes ◽  
The Impact

Abstract. Arctic permafrost landscapes have functioned as a global carbon sink for millennia. These landscapes are very heterogeneous, and the omnipresent waterbodies are a carbon source within them. Yet, only a few studies focus on the impact of these waterbodies on the landscape carbon budget. We compare carbon dioxide and methane fluxes from small waterbodies to fluxes from the surrounding tundra using eddy covariance measurements from a tower located between a large pond and semi-terrestrial vegetated tundra. When taking the open-water areas of small waterbodies into account, the carbon dioxide sink strength of the landscape was reduced by 11 %. While open-water methane emissions were similar to the tundra emissions, some parts of the studied pond's shoreline exhibited much higher emissions, underlining the high spatial variability of methane emissions. We conclude that gas fluxes from small waterbodies can contribute significantly to the carbon budget of arctic tundra landscapes. Consequently, changes in arctic hydrology and the concomitant changes in the waterbody distribution may substantially impact the overall carbon budget of the Arctic.

scholarly journals Sea-ice edge is more important than closer open water access for foraging Adélie penguins: evidence from two colonies

2020 ◽  
Vol 640 ◽  
pp. 215-230
Author(s):  
C Michelot ◽  
A Kato ◽  
T Raclot ◽  
K Shiomi ◽  
P Goulet ◽  
...  
Keyword(s):  
Sea Ice ◽  
Environmental Changes ◽  
Early Stage ◽  
Open Water ◽  
Gps Tracking ◽  
Ice Edge ◽  
Sea Ice Edge ◽  
Study Sites ◽  
The Impact ◽  
Adélie Penguins

Sentinel species, like Adélie penguins, have been used to assess the impact of environmental changes, and their link with sea ice has received considerable attention. Here, we tested if foraging Adélie penguins from 2 colonies in East Antarctica target the distant sea-ice edge or take advantage of closer open waters that are readily available near their colony. We examined the foraging behaviour of penguins during the incubation trips of females in 2016 and males in 2017, using GPS tracking and diet data in view of daily sea-ice data and bathymetry. In 2016-2017, sea-ice cover was extensive during females’ trips but flaw leads and polynyas were close to both study sites. Sea ice receded rapidly during males’ trips in 2017-2018. Despite close open water near both colonies in both years, females and males preferentially targeted the continental slope and the sea-ice edge to forage. In addition, there was no difference in the diet of penguins from both colonies: all penguins fed mostly on Antarctic krill and males also foraged on Antarctic silverfish. Our results highlight the importance of the sea-ice edge for penguins, an area where food abundance is predictable. It is likely that resource availability was not sufficient in closer open water areas at such an early stage in the breeding season. The behaviours displayed by the penguins from both colonies were similar, suggesting a common behaviour across colonies in Terre Adélie, although additional sites would be necessary to confirm this hypothesis.

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