scholarly journals Evaluation of Root-End Resections Performed by Er, Cr: YSGG Laser with and without Placement of a Root-End Filling Material

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
John Sullivan ◽  
Roberta Pileggi ◽  
Claudio Varella
Keyword(s):  
Methylene Blue ◽  
Epoxy Resin ◽  
Sem Analysis ◽  
The Other ◽  
Filling Material ◽  
Direct Influence ◽  
Ysgg Laser ◽  
Dye Penetration ◽  
Ah Plus ◽  
Imagej Software

Microleakage following root-end resections has a direct influence on the outcome of surgical endodontic procedures. This study compared the microleakage after root-end resections performed by the Er, Cr: YSGG laser or carbide burs with or without the placement of MTA, and evaluated the presence of microcracks and gaps at the interface of GP/MTA and the canal walls. Ninety single-rooted teeth were instrumented, obturated with GP and AH-Plus sealer, and divided into 3 experimental groups: (I) root-end resections were performed with the laser and G6 tips (parameters: 4.5 w, 30 pps, 20% water and 50% air); (II) Lindeman burs were used, without the placement of MTA; (III) the burs were used followed by root-end fillings with MTA, and one control (IV) of five unobturated roots resected with the burs. The samples were prepared for microleakage () and SEM () analysis. They were immersed in 1% methylene blue, decalcified, cleared, and evaluated for dye penetration () with the ImageJ software. Epoxy-resin replicas of the root-ends were analyzed by SEM for gaps () and microcracks. Microleakage results were , , and , for the laser (I), no root-end filling (II), and MTA (III) samples, respectively, (ANOVA ). The laser () and no root-end filling () samples presented gaps. Whereas, none was found in the MTA (ANOVA ). Microcracks were not observed. The MTA group demonstrated statistically less leakage and better adaptation to the canal walls when compared to the other groups. There was no correlation between the size of the gaps and the degree of microleakage.

scholarly journals Removal of Gutta-Percha/Zinc-Oxide-Eugenol Sealer or Gutta-Percha/Epoxy Resin Sealer from Severely Curved Canals: An In Vitro Study

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Santhoshini Reddy ◽  
Prasanna Neelakantan ◽  
Mohammad Ali Saghiri ◽  
Mehrdad Lotfi ◽  
Chandragiri Venkata Subbarao ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Epoxy Resin ◽  
File Systems ◽  
In Vitro Study ◽  
The Other ◽  
Filling Material ◽  
Gutta Percha ◽  
Bonferroni Adjustment ◽  
Zinc Oxide Eugenol

The aim of this study was to compare the cleanliness of root canal walls after retreatment using two rotary retreatment files to hand instruments in severely curved canals obturated with gutta-percha and two different sealers. Single rooted mandibular premolars (n=90) with root curvatures were instrumented and obturated with gutta-percha and an epoxy resin (Group 1,n=45) or zinc oxide eugenol sealer (Group 2,n=45). Following retreatment of the specimens (n=15ProTaper Universal Retreatment Files (Subgroup B) or R-Endo retreatment files (Subgroup C) after 1 month, split specimens were examined under a stereomicroscope and the percentage of remaining root filling material was statistically compared using one way ANOVA with Bonferroni adjustment for multiple comparisons (P=0.05). The R-Endo system performed significantly better than the other two file systems (P<0.05). None of the systems used in this study cleaned root canals thoroughly. The R-Endo system did provide cleaner walls when compared to the other instruments used. The type of root filling materials had an impact on the outcomes with all techniques.

scholarly journals An In Vitro Stereomicroscopic Evaluation of Bioactivity between Neo MTA Plus, Pro Root MTA, BIODENTINE & Glass Ionomer Cement Using Dye Penetration Method

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3159
Author(s):  
Mohmed Isaqali Karobari ◽  
Syed Nahid Basheer ◽  
Fazlur Rahman Sayed ◽  
Sufiyan Shaikh ◽  
Muhammad Atif Saleem Agwan ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Root Canal ◽  
Glass Ionomer Cement ◽  
Filling Material ◽  
Glass Ionomer ◽  
Dye Penetration ◽  
Anterior Teeth ◽  
Significant Difference ◽  
Ionomer Cement

The ideal root end filling material should form a tight seal in the root canal by adhering to the cavity walls. Several materials have been used for root end filling. The present study aims to find out and compare the bioactivity of Neo MTA Plus, Pro Root MTA White, BIODENTINE & glass ionomer cement as root end filling materials using 1% methylene blue as tracer. Materials and methods: 80 extracted human permanent maxillary anterior teeth were used in the study. They were divided into four groups. Specimens were sectioned transversely in the cervical area to separate the crown from the root. The root canal was obturated with gutta percha and zinc oxide eugenol sealers. Thereafter, each sample was resected apically by removing 3 mm of the apex and filled with different materials. Samples were kept in buffering solution at 37 °C until the recommended evaluation periods. The specimens were then suspended in 1% methylene blue for 24 h, prior to the analysis. The teeth were then sectioned, and dye penetration was examined, photographed, and evaluated under a stereomicroscope. Results: Vertical dye penetration showed significant differences across different groups. The minimum dye penetration was seen in Neo MTA plus followed by BIODENTINE, Pro Root MTA and maximum in GIC. There was no significant difference in dye penetration between Neo MTA plus and BIODENTINE both at fifteen days and one-month intervals. Conclusion: The present study suggests Neo MTA plus and BIODENTINE should be the preferred material for root end filling.

scholarly journals Antibacterial Activity of a New Ready-To-Use Calcium Silicate-Based Sealer

2020 ◽  
Vol 31 (6) ◽  
pp. 611-616
Author(s):  
Vanessa Maia Barbosa ◽  
André Pitondo-Silva ◽  
Mariana Oliveira-Silva ◽  
Antônio Secco Martorano ◽  
Claudia de Castro Rizzi-Maia ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Calcium Silicate ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Ah Plus ◽  
Agar Diffusion Method ◽  
Imagej Software ◽  
Endodontic Sealers

Abstract The aim of this study was to evaluate the antibacterial potential of a calcium silicate-based sealer (Bio-C Sealer, Angelus) against common bacteria in primary and secondary endodontic infections. Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus mutans were exposed to fresh Bio-C Sealer for 24 h by the agar diffusion method (n=5). Additionally, the antibacterial activity was investigated against E. faecalis and S. mutans biofilms (48 h old) grown in discs with 4 mm in diameter and 2 mm in height. (n=3) of set discs of Bio-C Sealer (Angelus), EndoFill (Dentsply-Mallefer), Sealer 26 (Dentsply), AH Plus (Dentsply), Sealapex (Sybron-Endo) and EndoSequence BC Sealer (Brasseler). The antibacterial activity was evaluated by colony forming unity (CFU) counting using ImageJ software. Data were compared by one-way ANOVA followed by Holm-Sidak test (a=5%). Fresh Bio-C Sealer exhibited antimicrobial activity against all bacteria evaluated by agar diffusion method, except for S. mutans. Set discs of all endodontic sealers tested showed similar CFU values for E. faecalis (p>0.05). S. mutans in biofilms showed higher susceptibility to EndoFill compared with the other sealers (p<0.05). In conclusion, the results indicate that fresh Bio-C Sealer does not inhibit S. mutans growth, but exhibits antibacterial activity against E. faecalis, S. aureus, P. aeruginosa and E. coli. After setting, the Bio-C Sealer exhibits an antimicrobial potential comparable to that of the other sealers evaluated in E. faecalis biofilm, but lower than that of EndoFill for S. mutans biofilm.

scholarly journals Evaluation of apical microleakage of teeth sealed with four different root canal sealers

2006 ◽  
Vol 14 (5) ◽  
pp. 341-345 ◽  
Author(s):  
Fábio Dultra ◽  
Juliana Machado Barroso ◽  
Laise Daniela Carrasco ◽  
Alexandre Capelli ◽  
Danilo M. Zanello Guerisoli ◽  
...  
Keyword(s):  
Root Canal ◽  
Methyl Salicylate ◽  
The Other ◽  
Root Canal Sealer ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
India Ink ◽  
Ah Plus ◽  
Root Canal Sealers

OBJECTIVES: to compare the apical sealing ability of four root canal sealers. MATERIAL AND METHODS: forty extracted human maxillary canines were instrumented 1 mm short of the anatomical apex and randomly assigned to four groups (n=10), according to the root canal sealer used for obturation: Endofill, AH Plus, EndoREZ and Epiphany. Root canals were obturated with gutta-percha points, except for the Epiphany group, in which resin points (Resilon) were used. The teeth were immersed in India ink for seven days and clarified using methyl salicylate. The extent of apical dye penetration was measured with a measuroscope in all aspects of the canal. RESULTS: AH Plus (0.02 mm ± 0.07), Epiphany (0.00 mm ± 0.00) and EndoREZ (0.32 mm ± 0.62) did not differ statistically to each other (p>0.01). EndoFill presented the highest dye penetration mean (0.83 mm ± 0.73) and was statistically different from the other sealers (p<0.01). CONCLUSION: the resin-based root canal sealers presented lesser apical microleakage than the zinc oxide and eugenol based sealer. No statistical differences were observed among resin based sealers.

scholarly journals Marginal Adaptation and Micropermeability of Class II Cavities Restored with Three Different Types of Resin Composites—A Comparative Ten-Month In Vitro Study

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1660
Author(s):  
Sevda Mihailova Yantcheva
Keyword(s):  
Methylene Blue ◽  
In Vitro Study ◽  
Sem Analysis ◽  
Marginal Adaptation ◽  
Resin Composites ◽  
Polymerization Shrinkage ◽  
Gingival Margin ◽  
Different Types ◽  
Ageing Period

The development of composite materials is subject to the desire to overcome polymerization shrinkage and generated polymerization stress. An indicator characterizing the properties of restorative materials, with specific importance for preventing secondary caries, is the integrity and durability of marginal sealing. It is a reflection of the effects of polymerization shrinkage and generated stress. The present study aimed to evaluate and correlate marginal integrity and micropermeability in second-class cavities restored with three different types of composites, representing different strategies to reduce polymerization shrinkage and stress: nanocomposite, silorane, and bulk-fill composite after a ten-month ageing period. Thirty standardized class ΙΙ cavities were prepared on extracted human molars. Gingival margins were 1 mm apical to the cementoenamel junction. Cavities were randomly divided into three groups, based on the composites used: FiltekUltimate-nanocomposite; Filtek Silorane LS-silorane; SonicFill-bulk-fill composite. All specimens were subjected to thermal cycles after that, dipped in saline for 10-mounds. After ageing, samples were immersed in a 2% methylene blue. Thus prepared, they were covered directly with gold and analyzed on SEM for assessment of marginal seal. When the SEM analysis was completed, the teeth were included into epoxy blocks and cut longitudinally on three slices for each cavity. An assessment of microleakage on stereomicroscope followed. Results were statistically analyzed. For marginal seal evaluation: F.Ultimate and F.Silorane differ statistically with more excellent results than SonicFill for marginal adaptation to the gingival margin, located entirely in the dentin. For microleakage evaluation: F.Ultimate and F.Silorane differ statistically with less microleakage than SonicFill. Based on the results obtained: a strong correlation is found between excellent results for marginal adaptation to the marginal gingival ridge and micropermeability at the direction to the axial wall. We observe a more significant influence of time at the gingival margin of the cavities. There is a significant increase in the presence of marginal fissures (p = 0.001). A significant impact of time (p < 0.000) and of the material (p < 0.000) was found in the analysis of the microleakage.

The influence of lithium on the competence of the ectoderm in Ambystoma mexicanum

Development ◽  
1964 ◽  
Vol 12 (2) ◽  
pp. 317-331
Author(s):  
D. O. E. Gebhardt ◽  
P. D. Nieuwkoop
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Ambystoma Mexicanum ◽  
The Other ◽  
Direct Influence ◽  
Amphibian Embryos ◽  
The Central Nervous System ◽  
The Subject ◽  
Morphogenetic Potential ◽  
Made In

The influence of lithium on the amphibian egg has been the subject of a number of investigations. From the work of Lehmann (1937), Töndury (1938), and Pasteels (1945) it is known that exposure of amphibian embryos to lithium results in a progressive cranio-caudal reduction of the central nervous system and a simultaneous conversion of the presumptive notochord into somites. Whereas these experiments were made with whole embryos, attempts have been made in recent years to localize the lithium effect by transplanting or explanting specific parts of the embryo. Gallera (1949), for instance, concluded from his experiments with transplants containing lithium treated presumptive chorda mesoderm, that lithium had reduced the ‘morphogenetic potential’ of this inductor. Lombard (1952), on the other hand, claimed that the susceptibility of amphibian eggs towards lithium was the result of the ion's direct influence on the ectoderm rather than on the presumptive archenteron roof.

Method for the Quantification of Alginate in Microcapsules

1993 ◽  
Vol 2 (5) ◽  
pp. 429-436 ◽  
Author(s):  
Jean-Pierre Hallé ◽  
Danielle Landry ◽  
Alain Fournier ◽  
Michèle Beaudry ◽  
Francois A. Leblond
Keyword(s):  
Methylene Blue ◽  
Alkaline Medium ◽  
High Sensitivity ◽  
Sodium Concentration ◽  
The Other ◽  
Sensitive Assay ◽  
Reliable Measurement ◽  
Alginate Concentration ◽  
Other Substances ◽  
Alginate Content

Alginate is a key reagent in the preparation of microcapsules for cell transplantation. To address the question of the intracapsular alginate concentration, a sensitive assay has been developed to quantify the alginate content of microcapsules. The method is based on the metachromatic change induced by alginate binding to the dye, 1,9-dimethyl methylene blue (DMMB). The assay has a high sensitivity and precision. It covers a wide concentration range enabling the measurement of alginate in dilute supernatants as well as in microcapsules. For the latter, the membrane is initially dissolved by incubating the microcapsules in an alkaline medium. The effect of potentially interfering substances (poly-l-lysine (PLL), citrate, chloride, sodium) and of pH has been studied. Poly-l-lysine interfered with the assay at pH 6.5 but not at pH 13. Interference by sodium augmented with increasing sodium concentration and reached a plateau at 200 mM. This problem was overcome by routinely adjusting all samples to 500 mM sodium. The other substances tested had a negligible effect on the assay. The reliable measurement of alginate with this new assay will allow the optimization of the intracapsular alginate concentration.

scholarly journals New TiO2/DSAT Immobilization System for Photodegradation of Anionic and Cationic Dyes

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Wan Izhan Nawawi Wan Ismail ◽  
S. K. Ain ◽  
R. Zaharudin ◽  
Ali H. Jawad ◽  
M. A. M. Ishak ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Thin Layer ◽  
Degradation Rate ◽  
Glass Plate ◽  
Sem Analysis ◽  
Adhesive Tape ◽  
Support Material ◽  
Organic Additives ◽  
Number Of Cycles ◽  
Reactive Red 4

A new immobilized TiO2technique was prepared by coating TiO2solution onto double-sided adhesive tape (DSAT) as a thin layer binder without adding any organic additives. Glass plate was used as support material to immobilized TiO2/DSAT. Two different charges of dyes were applied, namely, anionic reactive red 4 (RR4) and cationic methylene blue (MB) dyes. Photocatalytic degradation of RR4 and MB dyes was observed under immobilized TiO2/DSAT with the degradation rate slightly lower and higher, respectively, compared with TiO2in suspension mode. It was observed that DSAT is able to provide a very strong intact between glass and TiO2layers thus making the reusability of immobilized TiO2/DSAT be up to 30 cycles. In fact, a better photodegradation activity was observed by number of cycles due to increasing formation of pores on TiO2surface observed by SEM analysis.

scholarly journals Long-term evaluation of crown and root discoloration induced by different endodontic sealers

2018 ◽  
Vol 17 ◽  
pp. e18483
Author(s):  
Beatriz Serrato Coelho ◽  
Paula Pontes Garcia ◽  
Flávia Sens Fagundes Tomazinho ◽  
Leonardo Fernandes Cunha ◽  
Denise Piotto Leonardi ◽  
...  
Keyword(s):  
Analysis Of Variance ◽  
Root Canal ◽  
The Other ◽  
Tooth Crown ◽  
Root Canal Filling ◽  
Color Changes ◽  
Term Evaluation ◽  
Ah Plus ◽  
Endodontic Sealers

Aim: To evaluate crown and root discoloration promoted by different endodontic sealers after root canal filling. Methods: Eighty bovine incisors were prepared and filled with: Endofill, Sealer 26, AH Plus, and MTA Fillapex. Color was recorded using a spectrophotometer before endodontic treatment (T0) and at 24 hours (T1), seven days (T2), 30 days (T3), and 90 days (T4) after treatment. Analyses were performed on the middle and cervical regions of the crown, and on the cervical third of the root, immediately below the cementoenamel junction. The color alterations (ΔE) were calculated using Commission International de I’Eclairage (CIE) L*a*b parameters, and data were analyzed by analysis of variance (ANOVA) and Tukey’s test (p<0.05). Results: All sealers induced color alterations. Sealer 26 resulted in the smallest changes in color (E = 5.32). The other materials did not present statistical differences (AH Plus E = 6.98; MTA Fillapex E = 6.88; Endofill E = 6.41). Of the three regions analyzed, the largest discoloration was observed at the cervical third of the root (E=10.67). In terms of time, the largest ΔE values (E=7.72) were observed at T4. Color changes at T1 (E=5.88), T2 (E=6.10), and T3 (E=5.89) were statistically similar. Conclusions: All endodontic sealers promoted discoloration on the tooth crown and root.

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