scholarly journals Brazilian Green Propolis: Anti-Inflammatory Property by an Immunomodulatory Activity

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Joleen Lopes Machado ◽  
Anne Karine Martins Assunção ◽  
Mayara Cristina Pinto da Silva ◽  
Aramys Silva dos Reis ◽  
Graciomar Conceição Costa ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Pulmonary Inflammation ◽  
Oral Route ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Water Control ◽  
Cotton Pellet Granuloma ◽  
Anti Inflammatory ◽  
Acute And Chronic Inflammation ◽  
Cytokine Quantification

The immunomodulatory and anti-inflammatory activities of green propolis extracts fromApis melliferawere investigated using acute and chronic inflammation models. Swiss mice were anesthetized and a cotton pellet granuloma was implanted in subcutaneous tissue. Then the mice were divided into six groups and received apyrogenic water or different propolis extracts by oral route (5 mg/kg). According to the treatment the groups were designated as E1A, E1B, E10, E11, and E12. The control group received apyrogenic water. The treatment was performed by six days when the mice were killed. The blood and the bronchoalveolar lavage (BAL) were collected to measure the leukocyte recruitment. In acute pulmonary inflammation, Balb/c mice received lipopolysaccharide (LPS) ofEscherichia coliby intranasal route for three days. Concomitantly the mice received by oral route apyrogenic water (control) or E10 and E11 propolis extracts. BAL was performed to assess the inflammatory infiltrate and cytokine quantification. The results showed that the E11 extract has anti-inflammatory property in both models by the inhibition of proinflammatory cytokines and increase of anti-inflammatory cytokines suggesting an immunomodulatory activity.

scholarly journals Rubiadin exerts an acute and chronic anti-inflammatory effect in rodents

2023 ◽  
Vol 83 ◽  
Author(s):  
Romina Chitsaz ◽  
Atefeh Zarezadeh ◽  
Jinous Asgarpanah ◽  
Parvaneh Najafizadeh ◽  
Zahra Mousavi
Keyword(s):  
Inflammatory Activity ◽  
Control Group ◽  
Standard Drug ◽  
Test Models ◽  
Tnf Α ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Α Level ◽  
Post Hoc ◽  
Acute And Chronic Inflammation

Abstract: Rubiadin is identified as a bioactive anthraquinone that exists in some quinone rich plants. The current research was carried out to evaluate the potential anti-inflammatory impact of Rubiadin in acute and chronic inflammation test models in rodents. The anti-inflammatory activity of Rubiadin was examined in cotton pellet-induced granuloma and carrageenan-induced edema as chronic and acute inflammation models in rats. TNF-α level and histopathological changes were assessed using sampled foot tissue of rat in the acute model. Also, the IL-1β level was assessed in the chronic model. One-way ANOVA (post hoc Tukey’s) analysis was used for comparing the groups. Rubiadin (0.5 mg/kg, i.p.) induced a significant reduction in TNF α level and the paw edema compared to the control group in carrageenan test. Also, it was observed that the anti-inflammatory activity of Rubiadin (0.5 mg/kg, i.p.) is comparable to mefenamic acid (30 mg/kg, i.p.) as the standard drug. Rubiadin was effective in granuloma induced by cotton pellet concerning the granuloma and transudate formation amount. Rubiadin’s anti-inflammatory effects were associated with a significant IL-1β decrease in this model. The results suggest that Rubiadin as a natural compound can possess significant peripheral anti-inflammatory impacts.

scholarly journals Phytochemical-induced reduction of pulmonary inflammation during Klebsiella pneumoniae lung infection in mice

2014 ◽  
Vol 8 (07) ◽  
pp. 838-844 ◽  
Author(s):  
Shruti Bansal ◽  
Sanjay Chhibber
Keyword(s):  
Lung Tissue ◽  
Histopathological Examination ◽  
Curcuma Longa ◽  
Pulmonary Inflammation ◽  
Bacterial Load ◽  
Neutrophil Infiltration ◽  
Lung Infection ◽  
Control Group ◽  
Inflammatory Parameters ◽  
Anti Inflammatory

Introduction: Curcumin, a polyphenol derived from the herb Curcuma longa, has number of antioxidant, anti-inflammatory, antimicrobial, and anti-carcinogenic activities. Its anti-inflammatory property was here studied alone and in combination with clarithromycin in a mouse model of acute inflammation. Methodology: A total of 80 mice divided into four groups were used. Mice receiving curcumin and/or clarithromycin were fed orally with curcumin (150 mg/kg/day) for 15 days prior to infection, whereas clarithromycin was administered orally (30 mg/kg/day) 12 hours post infection. Simultaneously, the control group receiving only infection but no treatment was also set up. Bacterial load estimation, histopathological examination and analysis of inflammatory parameters was performed on various days for all groups. Results: Intranasal inoculation of bacteria resulted in significant increase in neutrophil infiltration along with increased production of various inflammatory mediators (malondialdehyde, myeloperoxidase, nitric oxide, TNFα) in lung tissue. Clarithromycin treatment significantly decreased the bacterial load and other inflammatory components in infected mice, but animals receiving curcumin alone or in combination with clarithromycin showed a much more significant (p < 0.05) reduction in neutrophil influx along with reduced levels of various inflammatory parameters. Though treatment with curcumin did not reduce the bacterial load, in combination with clarithromycin, both bacterial proliferation and lung tissue damage were checked. Conclusions: Though clarithromycin, because of its associated side effects, may not be the preferred treatment, it can be used in conjunction with curcumin. The latter as an adjunct therapy will help to down regulate the exaggerated state of immune response during acute lung infection.

scholarly journals Anti-inflammatory effect of hydroalcoholic extract of hibiscus rosa on acute and chronic inflammation

2016 ◽  
Vol 4 (2) ◽  
pp. 123
Author(s):  
Pankaj Verma ◽  
Swati Sharma ◽  
Vikas Kumar ◽  
Hema Chaudhary
Keyword(s):  
Chronic Inflammation ◽  
Soxhlet Extraction ◽  
Treated Group ◽  
Chronic Inflammatory Disease ◽  
Control Group ◽  
Hydroalcoholic Extract ◽  
Anti Inflammatory ◽  
Acute And Chronic Inflammation ◽  
Different Doses ◽  
Inflammatory Effect

Background: The present study was carried out to explore the efficiency of Indian herbal source from Hibiscus rosa against a chronic inflammatory disease. Hibiscus rosa belongs to Malvaceae, acts by suppression of inflammation mediators.Methods: Hydroalcoholic extract form Hibiscus rosa is prepared through soxhlet extraction and Diclofenac is used as the standard. Carrageenan and formaldehyde are administered to induce acute and chronic inflammation. Animals are divided into 6 groups with 6 animals each including Normal group, inflammatory control group, Diclofenac treated group and Hibiscus rosa treated group at different doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg.Results: Different concentrations of Hibiscus rosa treated groups i.e. 250 mg/kg (p<0.05), 500 mg/kg (p<0.05) and 1000 mg/kg (p<0.01) showed significant reduction in Paw edema as compared to controls. Significant reduction in Body weight was also observed in Hibiscus rosa treated groups. Hematological profiles of Hibiscus rosa treated group are satisfying and significant.Conclusion: Results showed significant anti-inflammatory effect of Hydroalcoholic extract of Hibiscus rosa and justifying its therapeutic role in inflammatory condition..

scholarly journals Anti-Inflammatory Activity ofDelonix regia(Boj. Ex. Hook)

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Vaishali D. Shewale ◽  
Tushar A. Deshmukh ◽  
Liladhar S. Patil ◽  
Vijay R. Patil
Keyword(s):  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Control Group ◽  
Delonix Regia ◽  
Rat Paw Edema ◽  
Phytochemical Investigation ◽  
Cotton Pellet Granuloma ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Different Doses

The present work was to evaluate the anti-inflammatory activity ofDelonix regialeaves (Family: Caesalpiniaceae). The powder ofDelonix regialeaves was subjected to extraction with ethanol in soxhlet extractor. The ethanol extract after preliminary phytochemical investigation showed the presence of sterols, triterpenoids, phenolic compounds and flavonoids. The anti-inflammatory activity was studied using carrageenan-induced rat paw edema and cotton pellet granuloma at a three different doses (100, 200, and 400 mg/kg b.w. p.o.) of ethanol extract. The ethanol extract ofDelonix regialeaves was exhibited significant anti-inflammatory activity at the dose of 400 mg/kg in both models when compared with control group. Indomethacin (10 mg/kg b.w. p.o) was also shown significant anti-inflammatory activity in both models.

scholarly journals Anti-Inflammatory Activity of Mimosa hamata (Willd.)

2019 ◽  
Vol 9 (5-s) ◽  
pp. 34-39
Author(s):  
N. I. Khan ◽  
B.C. Hatapakki
Keyword(s):  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Control Group ◽  
Albino Rats ◽  
Significant Activity ◽  
Dependent Manner ◽  
Paw Edema ◽  
Cotton Pellet ◽  
Cotton Pellet Granuloma ◽  
Anti Inflammatory

Anti-inflammatory activity of ethanolic extract of roots, stems and leaves Mimosa hamata was investigated at the doses of 200 and 400 mg/kg using carrageenan induced paw edema and cotton pellet granuloma technique in albino rats. The stem extracts showed significant activity in dose dependent manner as compared to control group. The observations suggested that the extract of M. hamata were effective in exudative and proliferative phases of inflammation i.e. in acute and chronic inflammation. The results obtained indicate that M. hamata has an anti-inflammatory activity that supports the folk medicinal use of the plant. Keywords: Mimosa hamata, anti-inflammatory activity, carrageenan induced paw edema, cotton pellet granuloma.

scholarly journals Evaluation of Methanol-Dichloromethane Extract of Stemonocoleus micranthus Harms (Fabaceae) Stem Bark for Anti-Inflammatory and Immunomodulatory Activities

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Florence Nwakaego Mbaoji ◽  
Akachukwu Marytheresa Onwuka ◽  
Sunday Onu ◽  
Ikechukwu Emmanuel Peter ◽  
Justus Amuche Nweze ◽  
...  
Keyword(s):  
Neutrophil Count ◽  
Lethal Dose ◽  
Stem Bark ◽  
Treated Group ◽  
Phagocytic Index ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Ear Oedema

Background. The stem bark decoction of Stemonocoleus micranthus Harms (Fabaceae) is most widely used traditionally as a remedy for various diseases such as malaria and boil. In this study, the anti-inflammatory and immunomodulatory activities of the methanol-dichloromethane extract (MDE) from the stem bark of the plant in rodents were evaluated. Methods. The carrageenan-induced rat paw oedema, cotton pellet-induced granuloma in rat, and xylene-induced ear oedema in mice were used to study the anti-inflammatory activity of methanol-dichloromethane extract of Stemonocoleus micranthus (MDESm) (100, 200, and 400 mg/kg). The effects of MDESm (100, 200, and 400 mg/kg) on cyclophosphamide-induced immunosuppression, neutrophil adhesion, carbon clearance, and haematological and biochemical parameters were carried out to study its immunomodulatory activity in mice. Result. MDESm (100 mg/kg, p.o.) significantly (p<0.05) inhibited carrageenan-induced oedema by 57.1% at 5th h posttreatment compared with control. At 100 mg/kg, p.o., MDESm significantly (p<0.05) reduced cotton pellet-induced granuloma by 39.28% and nonsignificantly reduced xylene-induced ear oedema by 34.1%. Treatment with MDESm (100 and 400 mg/kg) nonsignificantly abolished the neutropenia caused by cyclophosphamide with a percentage neutrophil reduction of 0 and −14.86%, respectively, while MDESm (200 mg/kg) and levamisole (50 mg/kg) had a nonsignificant reduction in neutrophil count (10.16 and 31.40%), respectively, all compared to the distilled water-treated group with a neutrophil count of −9.82%. MDESm at doses of 100 and 200 mg/kg increased phagocytic index by 0.0447 ± 0.00762 and 0.0466 ± 0.00703, respectively, although not significantly when compared to the control group with a value of 0.0226 ± 0.02117. There was a decrease in WBC and lymphocyte counts in MDESm- (200 mg/kg) treated group, suggesting immunosuppressive potential at this dose. MDESm caused a dose-dependent decrease in ALT and core liver enzymes, suggesting a hepatoprotective effect. The acute toxicity test revealed that MDESm is safe in mice with an oral lethal dose (LD50) of >5 g/kg. Conclusion. The methanol-dichloromethane extract of Stemonocoleus micranthus Harms possesses mild anti-inflammatory and immunomodulatory activities which may be more pronounced upon fractionation and purification. Therefore, more investigations are needed to explore these activities further.

scholarly journals Comparative Evaluation of Anti-Inflammatory Activity of Curcuminoids, Turmerones, and Aqueous Extract ofCurcuma longa

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ashish Subhash Bagad ◽  
Joshua Allan Joseph ◽  
Natarajan Bhaskaran ◽  
Amit Agarwal
Keyword(s):  
Aqueous Extract ◽  
Curcuma Longa ◽  
Volatile Oil ◽  
Inflammatory Activity ◽  
Bioactive Components ◽  
Traditional System ◽  
Cotton Pellet Granuloma ◽  
Anti Inflammatory ◽  
Acute And Chronic Inflammation ◽  
Dose Levels

Curcuma longais widely known for its anti-inflammatory activity in traditional system of medicine for centuries and has been scientifically validated extensively. The present study was conducted to evaluate the anti-inflammatory activity of curcuminoids and oil-free aqueous extract (COFAE) ofC. longaand compare it with that of curcuminoids and turmerones (volatile oil), the bioactive components ofC. longathat are proven for the anti-inflammatory potential. The activity against inflammation was evaluated in xylene-induced ear edema, cotton pellet granuloma models in albino Swiss mice and albino Wistar rats, respectively. The results showed that COFAE ofC. longaat three dose levels significantly(P≤0.05)inhibited inflammation in both models, as evidenced by reduction in ear weight and decrease in wet as well as dry weights of cotton pellets, when compared to the vehicle control. The COFAE ofC. longashowed considerable anti-inflammatory effects against acute and chronic inflammation and the effects were comparable to those of curcuminoids and turmerones.

Pretreatment with Methylprednisolone Improves Myocardial Protection during On-Pump Coronary Artery Bypass Surgery

2015 ◽  
Vol 18 (4) ◽  
pp. 171 ◽  
Author(s):  
Tolga Demir ◽  
Mehmet Umit Ergenoglu ◽  
Hale Bolgi Demir ◽  
Nursen Tanrikulu ◽  
Mazlum Sahin ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Coronary Artery Bypass ◽  
Myocardial Protection ◽  
Troponin T ◽  
Artery Bypass ◽  
Control Group ◽  
Study Group ◽  
Cabg Surgery ◽  
Anti Inflammatory ◽  
Pump Coronary Artery Bypass

<strong>Background</strong>: This study was undertaken to determine whether methylprednisolone could improve myocardial protection by altering the cytokine profile toward an anti-inflammatory course in patients undergoing elective coronary artery bypass grafting (CABG) surgery with cardiopulmonary bypass (CPB).<br /><strong>Methods</strong>: Forty patients who were scheduled for elective CABG surgery were randomized into two groups: the study group (n = 20), who received 1 g of methylprednisolone intravenously before CPB, and the control group (n = 20), who underwent a standard CABG surgery without any additional medication. Blood samples were withdrawn prior to surgery (T1) and then 4 hours (T2), 24 hours (T3), and 36 hours (T4) after CPB. Plasma levels of interleukin (IL)-6, IL-10, creatine kinase isoenzyme MB (CK-MB), cardiac troponin-t (cTnT), and blood glucose as well as neutrophil counts were measured at each sampling time. <br /><strong>Results</strong>: A comparison of patients between both groups revealed significantly high levels of IL-6 in the control group at T2, T3, and T4 with respect to T1 (T2: P &lt; .001; T3: <br />P &lt; .001; T4: P &lt; .001). IL-10 levels were significantly higher in the study group at T2 compared with the control group <br />(P = .007). CK-MB levels were significantly lower in the study group than in the control group at T4 (P = .001). The increase of cTnT was higher in the control group at T3 and T4 compared with the study group (T3: P = .002; T4: P = .001).<br /><strong>Conclusions</strong>: This study demonstrates that methylprednisolone is effective for ensuring better myocardial protection during cardiac surgery by suppressing the inflammatory response via decreasing the levels of IL-6 and by increasing anti-inflammatory activity through IL-10.<br /><br />

Valorization of the Pharmacological Potential of Phytochemical Compounds Contained in the Crude Extract of the Root of a Plant of Withania frutescens L

2019 ◽  
Author(s):  
A. EL Moussaoui ◽  
F. Jawhari ◽  
K. EL Ouahdani ◽  
I. Es-Safi ◽  
D. Bousta ◽  
...  
Keyword(s):  
Crude Extract ◽  
Control Group ◽  
Root Extract ◽  
Nacl Solution ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Pharmacological Potential

Our present study focuses on the evaluation of the analgesic, anti-inflammatory and healing activity of Withania frutescens L. The anti-inflammatory result has an inhibition percentage of 78.87% ± 7.08 at 450 mg/kg and 75.14% ± 6.39 at 400 mg/kg and 89.75% ± 3.44 for diclofenac (1%). When applied locally, the 10% cream has an inflammation inhibition of 96.87% ± 5.85 and 76.14% ± 7.88 for the 5% cream with 89.87 ± 6.20 of reference (Indomethacin). The abdominal contractions of rats treated with the root extract are significantly lower than those of the control group that received only physiological NaCl solution, with 41.20 ± 2.30 for the extract and 82.20 ± 5.04 for NaCl and 53.40 ± 4.94 for the reference. The healing activity of the studied extract records a percentage of contraction of about 93.20% ± 3.36 (Extract 10%), 84.50% ± 3.84 (Extract 5%), 48.47% ± 2.15 (control) and 81.88 ± 2.24 for the reference.

Assessment of biochemical determinants in Multiple Sclerosis patients following the oral administration of β-D-Mannuronic acid [M2000]

2020 ◽  
Vol 17 ◽  
Author(s):  
Mohamad Reza Nikouei Moghaddam ◽  
Monireh Movahedi ◽  
Maryam Bananej ◽  
Soheil Najafi ◽  
Nahid Beladi Moghadam ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Uric Acid ◽  
Vitamin D3 ◽  
Chronic Inflammatory Disease ◽  
Toxic Effects ◽  
Control Group ◽  
Therapeutic Effects ◽  
Mannuronic Acid ◽  
Anti Inflammatory ◽  
Multiple Sclerosis Patients

Background: Multiple sclerosis is an autoimmune chronic inflammatory disease of the central nervous system that can lead to some serious disabilities. Despite using various immunomodulatory and anti-inflammatory drugs that have therapeutic effects, they cannot reduce its progression completely, and have some unwanted side effects too. The immunomodulatory and anti-inflammatory effects of the β-D-Mannuronic acid [M2000] have been proven in several surveys, and the present research was designed to determine its toxicity and therapeutic effects in MS patients. Methods: This study was performed on 15 MS patients who took 25 mg/kg/day the oral form of the β-D-Mannuronic acid for six months, and 15 healthy people as a control group. Serum levels of Urea, Creatinine, GGT, Vitamin D3, Uric acid, and Anti-Phospholipids were compared to evaluate the therapeutic and possible toxic effects of this drug after this period. Results: Non- toxic effects through the study of Urea, Creatinine, GGT, and non-significant changes in Uric acid and AntiPhospholipids levels, besides a significant rise in Vitamin, D3 levels in the M2000 treated cases were found. Conclusions: Our results suggested that β-D-Mannuronic acid is a safe drug and has no toxicity when administered orally and also has some therapeutic effects in MS patients.

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