Antirheumatoid Arthritis Activities and Chemical Compositions of Phenolic Compounds-Rich Fraction fromUrtica atrichocaulis, an Endemic Plant to China

Urtica atrichocaulis, an endemic plant to China, is commonly used to treat rheumatoid arthritis even though its pharmaceutical activities and chemical constituents were not studied. Herein, we reported our investigations on the chemical compositions of the phenolic compounds-rich fraction fromU. atrichocaulis(TFUA) and their antirheumatoid arthritis activities. We found that the TFUA significantly inhibited the adjuvant-induced rats arthritis, carrageenin-induced rats paw edema, cotton pellet-induced mice granuloma, and the acetic acid-induced mice writhing response. Our phytochemical investigations on the TFUA resulted in the first-time isolation and identification of 17 phenolic constituents and a bis (5-formylfurfuryl) ether. The extensive HPLC analysis also revealed the chemical compositions of TFUA. Our further biological evaluation of the main phenolic components, individually and collectively, indicated that the antirheumatoid arthritis activities of TFUA were the combined effect of multiple phenolic constituents.

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Phenolic Compounds from An Algerian Endemic Species of Hypochaeris laevigata var. hipponensis and Investigation of Antioxidant Activities

Plants ◽

10.3390/plants9040514 ◽

2020 ◽

Vol 9 (4) ◽

pp. 514 ◽

Cited By ~ 1

Author(s):

Nabila Souilah ◽

Zain Ullah ◽

Hamdi Bendif ◽

Kamel Medjroubi ◽

Tahar Hazmoune ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Activities ◽

Quinic Acid ◽

Total Phenolic ◽

Antioxidant Action ◽

Endemic Plant ◽

Aerial Parts ◽

Phenolic Constituents ◽

First Time ◽

Phenolic And Flavonoid

Hypochaeris laevigata var. hipponensis (Asteraceae) is an endemic plant from Algeria. In the current study, we analyzed for the first time its chemical composition, especially phenolic constituents of dichloromethane (DCM), ethyl acetate (EA), and n-butanol (BuOH) fractionsof the aerial parts of Hypochaeris laevigata var. hipponensis by liquid chromatography-mass spectrometry (LC-MS/MS). The number of phenolic compounds detected in DCM, EA, and BuOH fractions were found to be 9, 20, and 15, respectively. More specifically, 12 phenolic acids were detected. Among them, quinic acid, chlorogenic acid, and caffeic acid were the most abundant ones. Meanwhile, only seven flavonoids were detected. Among them, rutin, apigetrin, and isoquercitrin were the major ones. We also determined the total phenolic and flavonoid contents, and fraction EA showed the highest values, followed by BuOH, and DCM fractions. Furthermore, the antioxidant action was dictated by five methods and the tested plant fractions demonstrated a noteworthy antioxidant action.

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Chemical Constituents of the Lichen Cladina macaronesica

Zeitschrift für Naturforschung C ◽

10.1515/znc-1991-1-203 ◽

1991 ◽

Vol 46 (1-2) ◽

pp. 12-18 ◽

Cited By ~ 8

Author(s):

Antonio G . González ◽

Jaime Bermejo Barrera ◽

Elsa Ma Rodríguez Pérez ◽

Consuelo E. Hernández Padrón

Keyword(s):

Natural Products ◽

Phenolic Compounds ◽

Silver Nitrate ◽

Silica Gel ◽

Column Chromatography ◽

Chemical Constituents ◽

Usnic Acid ◽

Acetone Extract ◽

First Time

Column chromatography of the acetone extract of the lichen Cladina macaronesica (Sephadex LH-20, silica gel and silver nitrate-im pregnated silica gel) afforded eight triterpenes identified by chemical and spectral m eans. α-Amyrenone, lupenone, taraxerol, taraxerone and /so-arborinol acetate were isolated for the first time from lichens and (-)-usnic acid and five mononuclear phenolic compounds were also obtained, four for the first time as natural products. The possible transformation of perlatolic acid into these phenolic compounds is briefly outlined.

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Polyphenolic Extract from Maple Syrup Potentiates Antibiotic Susceptibility and Reduces Biofilm Formation of Pathogenic Bacteria

Applied and Environmental Microbiology ◽

10.1128/aem.00239-15 ◽

2015 ◽

Vol 81 (11) ◽

pp. 3782-3792 ◽

Cited By ~ 31

Author(s):

Vimal B. Maisuria ◽

Zeinab Hosseinidoust ◽

Nathalie Tufenkji

Keyword(s):

Antimicrobial Activity ◽

Phenolic Compounds ◽

Antibiotic Susceptibility ◽

Biofilm Formation ◽

Multiple Drug Resistance ◽

Content Type ◽

Maple Syrup ◽

Starting Point ◽

Phenolic Components ◽

Phenolic Constituents

ABSTRACTPhenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple syrup extract (PRMSE). PRMSE exhibited antimicrobial activity as well as strong synergistic interaction with selected antibiotics against Gram-negative clinical strains ofEscherichia coli,Proteus mirabilis, andPseudomonas aeruginosa. Among the phenolic constituents of PRMSE, catechol exhibited strong synergy with antibiotics as well as with other phenolic components of PRMSE against bacterial growth. At sublethal concentrations, PRMSE and catechol efficiently reduced biofilm formation and increased the susceptibility of bacterial biofilms to antibiotics. In an effort to elucidate the mechanism for the observed synergy with antibiotics, PRMSE was found to increase outer membrane permeability of all bacterial strains and effectively inhibit efflux pump activity. Furthermore, transcriptome analysis revealed that PRMSE significantly repressed multiple-drug resistance genes as well as genes associated with motility, adhesion, biofilm formation, and virulence. Overall, this study provides a proof of concept and starting point for investigating the molecular mechanism of the reported increase in bacterial antibiotic susceptibility in the presence of PRMSE.

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Chemical Constituents of the Different Parts of Colchicum micranthum and C. chalcedonicum and their Cytotoxic Activities

Natural Product Communications ◽

10.1177/1934578x1801300506 ◽

2018 ◽

Vol 13 (5) ◽

pp. 1934578X1801300 ◽

Cited By ~ 2

Author(s):

Gizem Gulsoy-Toplan ◽

Fatih Goger ◽

Ayca Yildiz-Pekoz ◽

Simon Gibbons ◽

Gunay Sariyar ◽

...

Keyword(s):

Phenolic Compounds ◽

Familial Mediterranean Fever ◽

Chemical Constituents ◽

Seed Extract ◽

Cytotoxic Activities ◽

A549 Cell Line ◽

Mediterranean Fever ◽

High Cytotoxicity ◽

First Time ◽

Different Parts

Colchicum species, which have been widely used as a medication for years, still remain important in treatment of Familial Mediterranean Fever, gout and Behcet's Disease. In the present work, alkaloids, phenolic compounds and cytotoxic activities from different parts of two Colchicum species, namely C. chalcedonicum and C. micranthum were investigated for the first time. From different parts of two species, alkaloids were isolated and colchicine concentrations were also determined by HPLC. The methanol extracts were investigated for their cytotoxic activity against the A549 cell line using the MTT and LDH methods. Additionally, the phenolic compounds of each extract were investigated by LC–MS/MS. Six alkaloids, namely colchicine, colchifoline, 2-demethylcolchicine, demecolcine, 4-hydroxycolchicine and N-deacetyl- N-formylcolchicine were isolated from different parts of two species. The autumn corm of C. micranthum possessed the highest amount of colchicine among all extracts. All extracts showed high cytotoxicity, while the highest toxicity was determined in the seed extract. According to the LC–MS/MS analysis, 19 phenolic compounds were shown to be present. This is the first study which highlights that the seeds of C. chalcedonicum and autumn corms of C. micranthum could be valuable for the pharmaceutical industry to obtain colchicine and other tropolone alkaloids.

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A New Colchicinoid from Colchicum tauri, an Unexplored Meadow Saffron Native to Jordan

Natural Product Communications ◽

10.1177/1934578x0600100203 ◽

2006 ◽

Vol 1 (2) ◽

pp. 1934578X0600100 ◽

Cited By ~ 3

Author(s):

Feras Q. Alali ◽

Amani S. Ma'aya'h ◽

Ahmad Alkofahi ◽

Amjad Qandil ◽

Chen Li ◽

...

Keyword(s):

Brine Shrimp ◽

2D Nmr ◽

Biological Evaluation ◽

Preparative Hplc ◽

Low Resolution ◽

Isolation And Identification ◽

Brine Shrimp Lethality ◽

Chromatographic Techniques ◽

General Toxicity ◽

First Time

This study reports the isolation, characterization, and biological evaluation of constituents of Colchicum tauri Siehe ex Stef. (Colchicaceae), an unexplored Colchicum species native to Jordan. Methanolic extraction, crude alkaloid fractionation, and separation using several different chromatographic techniques (open columns, preparative TLC, and semi-preparative HPLC), as directed by the brine shrimp lethality test (BST), resulted in the isolation and identification of nine compounds: (-)-colchicine (1), (-)-2-demethylcolchicine (2), (-)-3-demethylcolchicine (3), (-)-demecolcine (4), (-)- N-methyldemecolcine (5), (-)-3-demethyl- N-methyldemecolcine (6), β-lumicolchicine (7), 3-demethyl-β-lumicolchicine (8), and apigenin (9). Compounds 1–5 and 7–8 were isolated for the first time from this species, while compound (6) represents a new colchicinoid. The structure elucidations were achieved using a series of spectroscopic and spectrometric techniques, principally 1D-NMR (1H, and 13C), 2D-NMR (COSY, ROESY, HMBC, and HSQC), and low resolution EI-MS. All compounds were tested for general toxicity using the BST, and compounds 1 and 4 were found to be the most potent, with LC50 values of 2.5 and 8.1 μg/mL, respectively.

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Chemical Constituents of Cichorium intybus and their Inhibitory Effects against Urease and α-Chymotrypsin Enzymes

Natural Product Communications ◽

10.1177/1934578x1100600817 ◽

2011 ◽

Vol 6 (8) ◽

pp. 1934578X1100600

Author(s):

Sumayya Saied ◽

Shazia Shah ◽

Zulfiqar Ali ◽

Ajmal Khan ◽

Bishnu P. Marasini ◽

...

Keyword(s):

Chemical Constituents ◽

2D Nmr ◽

Cichorium Intybus ◽

Spectroscopic Techniques ◽

Inhibitory Effects ◽

Isolation And Identification ◽

Aerial Parts ◽

Phytochemical Investigation ◽

Hydroxyphenylacetic Acid ◽

First Time

Phytochemical investigation of the aerial parts of Cichorium intybus L. resulted in the isolation and identification of two new natural metabolites, 2,6-di[but-3( E)-en-2-onyl]naphthalene (1), and 3,3′,4,4′-tetrahydroxychalcone (2), along with nine known compounds. Their structures were determined by spectroscopic techniques including 1D and 2D NMR. The known compounds were identified as scopoletin (3), 4-hydroxyphenylacetic acid (4), 3-hydroxy-4-methoxybenzoic acid (5), 4,4′-dihydroxychalcone (6), 6,7-dihydroxycoumarine (7), 1-triacontanol (8), lupeol (9), β-sitosterol (10), and β-sitosterol-3- O-β-glucopyranoside (11). Compounds 4-6 and 8 are reported for the first time from C. intybus. Compounds 2 and 3 showed weak inhibitory activities against urease and α-chymotrypsin enzymes, respectively.

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Study of the phenolic compounds of the dry extract of Thymus crebrifoli ususing a combined HPLC–UV and HPLC-ESI-MS/MS method

Bulletin of the Karaganda University Chemistry series ◽

10.31489/2021ch2/18-23 ◽

2021 ◽

Vol 102 (2) ◽

pp. 18-23

Author(s):

S.A. Ivasenko ◽

◽

K.K. Shakarimova ◽

A.B. Bokayeva ◽

A.B. Marchenko ◽

...

Keyword(s):

Phenolic Compounds ◽

Wide Spectrum ◽

Herbal Medicines ◽

Point Of View ◽

Endemic Plant ◽

Plant Materials ◽

Dry Extract ◽

Raw Plant Materials ◽

Double Extraction ◽

First Time

In world practice interest in herbal medicines is noticeably increasing every year. From this point of view, plants of the ThymusL. genus of the Lamiaceaefamily are of undoubted interest. Previously we have obtained a dry extract from the aerial part of an endemic plant of the flora of Kazakhstan Thymus crebrifoliusKlokovfor the first time by double extraction of raw plant materials with 70% ethanol using ultrasound. Dry extract of Thymus crebrifoliushas a wide spectrum of antimicrobial activity, including against Helicobacter pylori, while it is not toxic, and can be used as an antimicrobial agent. The article presents the results of a study of the composition of phenolic compounds of dry extract of Thymus crebrifoliususing a combined HPLC-UV and HPLC-MS/MS method. 12 phenolic compounds have been identified and quantified in the dry extract of Thy-mus crebrifolius. Four of them are phenolic acids, and eight are flavonoids. The dominant phenolic compounds are luteolin-7-O-glucoside (109.00mg g−1),rosmarinic acid (30.98mg g−1), naringenin (24.84 mg g−1), epicat-echin (9.98mg g−1),myricetin (6.15mg g−1) and gallic acid (3.41mg g−1). The results of chromatographic analysis will be used to standardize drugs based on dry extract of Thymus crebrifolius.

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Phenolic Compounds from Centaurea horrida L Growing in Lebanon

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/ijpapr.v9i1.8030 ◽

2017 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Rima Boukhary ◽

Maha Aboul-ElA ◽

Othman Al-Hanbali ◽

Abdalla El-Lakany

Keyword(s):

Phenolic Compounds ◽

Middle East ◽

Ethyl Acetate ◽

Caffeic Acid ◽

Folk Medicine ◽

Isolation And Identification ◽

Phytochemical Investigation ◽

First Time

Centaurea horrida has been used in folk medicine for many purposes as diuretic, antiinflammatory, hypotensive, antidiarrhetic, mild astringent, bitter tonic, stomachic, digestive, and emmenagogue and in cosmetics. An infusion of this plant is used to treat diabetes and digestive problems Also it is used as a wash for tired eyes and in conjunctivitis. C.horrida grown in Lebanon is widely used in the treatment of diarrhea, as antihypertensive and as hypoglycemic. Phytochemical investigation of the species grown in Lebanon led to the isolation and identification of five phenolic compounds isolated from methanol, ethyl acetate and butanol fractions of Centaurea extracts. Their structures were determined by NMR, MS, UV and IR methods. It is important to mention that the isolated flavonoids fisetin, hispidulin, quercetin, quercetin -3-D- galactoside and caffeic acid were isolated for the first time from Centaurea horrida. L growing in the Middle East.

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Chemical Constituents of Adesmia Boronioides and Evaluation of their Antioxidant Activity

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2015.8.3.5 ◽

2015 ◽

Vol 8 (3) ◽

pp. 2919-2223

Author(s):

Orlando Muñoz ◽

C Delporte

Keyword(s):

Chemical Constituents ◽

Antioxidant Properties ◽

2D Nmr ◽

Isolation And Identification ◽

Ongoing Research ◽

Phytochemical Investigation ◽

Kaempferol Glycoside ◽

Pharmacologically Active ◽

Pharmacologically Active Compounds ◽

First Time

As part of our ongoing research into the pharmacologically active compounds of Chilean flora, here we did phytochemical investigation of Adesmia boronioides Hook.f (fabaceae). (Papilonaceae) leaves. This effort has led to isolation and identification of liqueritigenin, isoliquiritigenin, sesamin, isodrimenin and a kaempferol glycoside, kaempferol 3-O-β-D-[galactopyranosyl (1–2) rhamnoside]-7-O –α-L-rhamnopyranoside.Compound structures were identified by 1D- and 2D-NMR experiments, mass and UV spectrometry and comparison with data from the literature. This is the first time these compounds have been isolated from this plant’s leaves.These active constituents have been studied further pharmacologically. Antioxidant properties in A. boronioides leaves were determined.

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Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri (Boiss) Dőrfler

Open Life Sciences ◽

10.2478/s11535-012-0021-8 ◽

2012 ◽

Vol 7 (2) ◽

pp. 327-333 ◽

Cited By ~ 3

Author(s):

Pavle Mašković ◽

Jelena Maksimović ◽

Vuk Maksimović ◽

Jelena Blagojević ◽

Mladen Vujošević ◽

...

Keyword(s):

Phenolic Compounds ◽

Endemic Species ◽

Hplc Analysis ◽

Biological Activities ◽

Chemical Constituents ◽

Ethanolic Extract ◽

Antimicrobial Activities ◽

Endemic Plant ◽

Genotoxic Effects ◽

Bacteria And Fungi

AbstractThe objective of this study was to evaluate the efficacy of the ethanolic extract of endemic plant Halacsya sendtneri in inhibiting the growing of the test fungi and bacteria as well as to determine its genotoxic potential and toxicity using the Allium anaphase-telophase assay. Minimum inhibitory concentrations (MIC) were determined for 15 indicator strains of pathogens, representing both bacteria and fungi. The highest susceptibility to the ethanolic extract of H.sendtneri was exhibited by Pseudomonas glycinea (FSB4), (MIC=0.09 mg/ml) among the bacteria, and by Phialophora fastigiata (FSB81), (MIC=1.95 mg/ml) among the fungi. The composition of H. sendtneri extracts was also determined using HPLC analysis. Rosmarinic acid was found to be the dominant phenolic compound. The Allium anaphase-telophase genotoxicity assay revealed that the ethanolic extract of H. sendtneri at concentrations of 31.5 mg/l and below does not produce toxic or genotoxic effects. This is the first report of chemical constituents, genotoxic and antimicrobial activities of the endemic species, H. sendtneri.

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