scholarly journals Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Selvaraj Raja ◽  
Vytla Ramachandra Murty
Keyword(s):  
Soluble Proteins ◽  
Fractional Factorial ◽  
Tannery Wastewater ◽  
Sequential Optimization ◽  
Precipitation Method ◽  
Phase System ◽  
Optimization Strategy ◽  
Two Phase ◽  
Peg 6000 ◽  
Aqueous Two Phase Systems

Aqueous two-phase system (ATPS) composed of polyethylene glycol 6000 (PEG 6000) and sodium citrate (SC) has been proposed to recover the valuable soluble proteins from tannery wastewater. A sequential optimization strategy which included fractional factorial design (fFD) and central composite design (CCD) was employed to enhance the recovery. From this strategy, a second-order polynomial model was obtained for the protein recovery and it was validated. The optimum recovery was found as 93.46% when pH, NaCl concentration, and temperature were kept at 7.5, 0.1 M, and 33°C, respectively, for a phase system composed of 20% (w/w) PEG 6000-15% (w/w) SC. Thus the proposed ATPS can serve as an alternative to the conventional precipitation method to recover the soluble proteins from tannery wastewater.

scholarly journals Extraction and Purification of Capsaicin from Capsicum Oleoresin Using a Combination of Tunable Aqueous Polymer-Phase Impregnated Resin (TAPPIR) Extraction and Chromatography Technology

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3956 ◽  
Author(s):  
Lu ◽  
Cui
Keyword(s):  
Sodium Citrate ◽  
Extraction Process ◽  
Fractional Factorial ◽  
Phase System ◽  
Polymer Phase ◽  
Macroporous Resin ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Extraction And Purification ◽  
Extraction Step

Capsaicin, which mainly comes from pepper, exhibits anticancer, antioxidant, and anti-obesity properties. This work aims to construct a comprehensive technology for the extraction and purification of capsaicin from capsicum oleoresin. The tunable aqueous polymer phase impregnated HZ816 resins were selected in extraction step. In the extraction process, 3 g of impregnated HZ816 macroporous resin was employed per system. The results showed that a higher molecular weight of Polyethylene glycol (PEG) and 1-ethyl-3-methyl imidazolium acetate ([Emim] [OAc]) are more beneficial to the improvement of the yield of capsaicin. Screening experiment using fractional factorial designs indicated that the amount of sample loading, pH, and concentration of [Emim] [OAc] and PEG 6000 significantly affect the yield of capsaicin. Mathematical models of capsaicin yield in tunable aqueous polymer-phase impregnated resins were established and optimum condition was obtained using response surface methodology. The optimum impregnated phase was the polymer phase of an aqueous two-phase system which contained 18.5% (w/w) PEG6000, 15% (w/w) sodium citrate, and 10% (w/w) [Emim] [OAc] at pH 6.5. Under the optimal conditions, the yield of capsaicin reached 95.82% when the extraction system contains 0.25 g capsicum oleoresin. Ultimately, capsaicinoids extract was purified by reverse-phase resin (SKP-10-4300) chromatographic column. The capsaicin recovery and purity achieved 85% and 92%, respectively.

Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures

Lab on a Chip ◽  
2015 ◽  
Vol 15 (11) ◽  
pp. 2437-2444 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Steven G. Jones ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Interfacial Tension ◽  
Phase System ◽  
Flow Focusing ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microfluidic Flow ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ultralow Interfacial Tension

Simple microfluidic flow focusing generation of droplets from ultralow interfacial tension aqueous two phase systems (ATPS).

Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System

2011 ◽  
Vol 138-139 ◽  
pp. 995-1001 ◽  
Author(s):  
Wei Jie Wang ◽  
Xiao Le Zhang ◽  
Chang Bo Xu ◽  
Hong Yan Cheng
Keyword(s):  
High Purity ◽  
Separation Process ◽  
Phase System ◽  
Two Phase ◽  
Sodium Tartrate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Optimized Conditions ◽  
Potassium Sodium Tartrate ◽  
Partitioning Behavior

In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

Gas Column/Mass Spectrometry Analysis of Bio Oil Treated by Aqueous Two Phase System

2011 ◽  
Vol 236-238 ◽  
pp. 54-58
Author(s):  
Shao Peng Liu ◽  
Zhi Min Zong ◽  
Shan Shan Zhang ◽  
Yue Xu ◽  
Gui Zhen Gong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Spectrometry Analysis ◽  
Bio Oil ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ethanol System

Bio oil aqueous two phase systems were formed with ethanol and the salts of (NH4)2SO4, K2HPO4 and NaH2PO4 in bio oil on the ratio of 10:5:10(by mass). After phase separation, both the upper and the lower layers were analyzed by gas column/mass spectrometry (GC/MS). As a result, both K2HPO4/ethanol and NaH2PO4/ethanol system can enrich phenols and levoglucosan in the upperand lower layers,respectly, while NaH2PO4/ethanol system seldom changes the components of bio oil; the (NH4)2SO4/ethanol system is quit different with the other two and we have detected some components with nitrogen. Hence, NaH2PO4/ethanol is an advantage aqueous two phase system for bio oil treatment.

scholarly journals Extraction of Fe (III) and Zn (II) by ionic liquids in a system based on polypropylene glycol 425 and sodium chloride.

2020 ◽  
Vol 11 (3-2020) ◽  
pp. 72-75
Author(s):  
I. V. Zinov’eva ◽  
◽  
M. I. Fedorova ◽  
Yu. A. Zakhodyaeva ◽  
A.A. Voshkin ◽  
...  
Keyword(s):  
Experimental Study ◽  
Ionic Liquids ◽  
Sodium Chloride ◽  
Phase System ◽  
Polypropylene Glycol ◽  
Two Phase ◽  
Study Results ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Extraction Of Metals

This work presents the experimental study results of Fe(III) and Zn(II) extraction by ionic liquids based on trioctylmethylammonium chloride and rhodanide in an aqueous two-phase system based on polypropylene glycol 425 and sodium chloride. Based on the results obtained, it was concluded that these extractants are effective for the extraction of metals from aqueous solutions, and aqueous two-phase systems represent a “green” alternative to traditional extraction systems.

Passive droplet generation in aqueous two-phase systems with a variable-width microchannel

Soft Matter ◽  
2019 ◽  
Vol 15 (23) ◽  
pp. 4647-4655 ◽  
Author(s):  
Daeho Choi ◽  
Eunjeong Lee ◽  
Sung-Jin Kim ◽  
Minsub Han
Keyword(s):  
Droplet Generation ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Passive droplet generation for an aqueous two-phase system (ATPS) was performed with a fracture-based variable microchannel.

scholarly journals Optimized Isolation of Extracellular Vesicles From Various Organic Sources Using Aqueous Two-Phase System

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Oğuz Kaan Kırbaş ◽  
Batuhan Turhan Bozkurt ◽  
Ayla Burçin Asutay ◽  
Beyza Mat ◽  
Bihter Ozdemir ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Cost Effective ◽  
Drug Carriers ◽  
Phase System ◽  
Phenol Red ◽  
Two Phase ◽  
Acid Acid ◽  
Aqueous Two Phase Systems ◽  
Specialized Equipment ◽  
Phase Systems

AbstractFrom biomarkers to drug carriers, Extracellular Vesicles (EVs) are being used successfully in numerous applications. However, while the subject has been steadily rising in popularity, current methods of isolating EVs are lagging behind, incapable of isolating EVs at a high enough quantity or quality while also requiring expensive, specialized equipment. The “isolation problem” is one of the major obstacles in the field of EV research - and even more so for their potential, widespread use for clinical diagnosis and therapeutic applications. Aqueous Two-Phase Systems (ATPS) has been reported previously as a promising method for isolating EVs quickly and efficiently, and with little contaminants - however, this method has not seen widespread use. In this study, an ATPS-based isolation protocol is used to isolate small EVs from plant, cell culture, and parasite culture sources. Isolated EVs were characterized in surface markers, size, and morphological manner. Additionally, the capacity of ATPS-based EV isolation in removing different contaminants was shown by measuring protein, fatty acid, acid, and phenol red levels of the final isolate. In conclusion, we have shown that EVs originating from different biological sources can be isolated successfully in a cost-effective and user-friendly manner with the use of aqueous two-phase systems.

Partitioning Behavior of Lysozyme and α-lactalbumin in Aqueous Two-Phase System Formed by Ionic Liquids and Potassium Phosphate

2017 ◽  
Vol 13 (10) ◽  
Author(s):  
Vanessa S. Sampaio ◽  
Renata C. F. Bonomo ◽  
Cristiane M. Veloso ◽  
Rita C. S. Sousa ◽  
Evaldo C. S. Júnior ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Partition Coefficients ◽  
Potassium Phosphate ◽  
Inorganic Salts ◽  
Phase System ◽  
Two Phase ◽  
Separation Processes ◽  
Different Temperatures ◽  
Aqueous Two Phase Systems ◽  
Increasing Temperature

AbstractNowadays ionic liquids (ILs), because of their “green” characteristics, have been used for analytical and separation processes. Therefore the partitioning of lysozyme and α-lactalbumin using aqueous two-phase systems (ATPSs) composed of an ionic liquid (chloride 1-ethyl-3-methylimidazolium) and inorganic salts (K2HPO4, KH2PO4) was studied. Phase equilibrium diagrams were obtained to explore the effect of the different temperatures (293.15, 303.15, 313.15, and 323.15) K and pH (7.5, 8.0, and 8.5) used for the partitioning studies. For both proteins, partition coefficients decreased with increasing temperature. The pH influenced the partition coefficients of lysozyme and α-lactalbumin. The thermodynamic parameters (ΔH◦, ΔS◦, ΔG◦) indicate thermodynamic differences between the partitioning of lysozyme and α-lactalbumin in this system. The ΔH◦, ΔS◦, and ΔG◦values of the process studied showed that this process is spontaneous. This work demonstrates the possible use of ATPSs with ILs and inorganic salts as a methodology for the partitioning of lysozyme and α-lactalbumin.

scholarly journals The extraction of Zn(II) in aqueous PEG (1550)-(NH4)2SO4 two-phase system using Cl- ions as extracting agent

2007 ◽  
Vol 72 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Laura Bulgariu ◽  
Dumitru Bulgariu
Keyword(s):  
Aqueous Solutions ◽  
Extraction Efficiency ◽  
Phase Variation ◽  
Chloride Ions ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Experimental Parameters ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

The extraction of Zn(II) in an aqueous PEG (1550) - (NH4)2SO4 two-phase system as a function of several experimental parameters was studied. PEG-based aqueous two-phase systems are composed of two immiscible phases: a polymer-rich phase and a salt-rich phase, which can be used for extraction experiments. In the absence of a suitable extracting agent, for the system consisting of a mixture of equal volumes of 40 mass% PEG and 40 mass% (NH4)2SO4 aqueous solutions, Zn(II) remained predominantly in the salt-rich phase. Variation of the pH of the salt stock solution did not change very much the extraction efficiency. By adding chloride ions, an enhancement of the Zn(II) extraction was observed. The Zn(II) extraction efficiency in presence of Cl- depends on the acidity of the salt stock solution and on the concentration of chloride ions added into the system.

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