scholarly journals Complements C3 and C5 Individually and in Combination Increase Early Wound Strength in a Rat Model of Experimental Wound Healing

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Hani Sinno ◽  
Meenakshi Malhotra ◽  
Justyn Lutfy ◽  
Barbara Jardin ◽  
Sebastian Winocour ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Sham Group ◽  
Breaking Strength ◽  
The Other ◽  
Systemic Effects ◽  
Skin Wounds ◽  
Collagen Deposition ◽  
Hemostatic Effect ◽  
Early Wound

Background. Complements C3 and C5 have independently been shown to augment and increase wound healing and strength. Our goal was to investigate the combinatorial effect of complements C3 and C5 on wound healing. Methods. Each rat served as its own control where topical collagen was applied to one incision and 100 nM of C3 and C5 in collagen vehicle was applied to the other incision (n=6). To compare between systemic effects, a sham group of rats (n=6) was treated with collagen alone on one wound and saline on the other. At day 3, the tissue was examined for maximal breaking strength (MBS) and sectioned for histological examination. Results. There was a statistically significant 88% increase in MBS with the topical application of C3C5 when compared to sham wounds (n<0.05). This was correlated with increased fibroblast and collagen deposition in the treated wounds. Furthermore, there appeared to be an additive hemostatic effect with the C3C5 combination. Conclusions. The combination of complements C3 and C5 as a topical application drug to skin wounds significantly increased wound healing maximum breaking strength as early as 3 days.

Pengaruh Luka Iris Pada Tikus Dengan Paparan Hidrogen Peroxida 3% terhadap Epitelisasi dan Pembentukan Eksudat pada Permukaan Epitel Kulit.

2020 ◽  
Vol 17 (2) ◽  
pp. 172
Author(s):  
HARMAN AGUSAPUTRA ◽  
MARIA SUGENG ◽  
AYLY SOEKAMTO ◽  
ATIK WULANDARI
Keyword(s):  
Hydrogen Peroxide ◽  
Wound Healing ◽  
The Other ◽  
Delayed Wound Healing ◽  
Peroxide Group ◽  
Hemostatic Effect ◽  
Significant Difference ◽  
Negative Effect ◽  
Wound Healing Effect ◽  
Epithelial Growth

<p><strong>Abstract</strong></p><p><strong>Background:</strong> Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as antiseptic has been used frequently to clean woundsin in hospitals and clinics. Hydrogen peroxide has the effectof strong oxidative that can kill pathogens. It can clean up debris and necrotic tissuesin wounds. Hydrogen peroxidealso has hemostatic effect that can help to stop bleeding. Besides antiseptic effects, hydrogen peroxide i s suspected of having negative effect in wound healing. Hydrogen peroxide presumably could cause delayed wound healing by exudate formation and delayed epithelial growth.</p><p><strong>Method</strong>: This study was conducted in the laboratory using 48 white mice that were divided into 2 groups. All the mice were purposely wounded. Afterwards in one group the wounds were clean up using hydrogen peroxide, while in the other group without hydrogen peroxide as control. The wounds of both groups were observed on day 1, day 3 and day 7. On day 1 and day 3, both groups did not show significant difference.</p><p><strong>R</strong><strong>esult</strong> : on day 7 showed that the wound healing in hydrogen peroxide group were delayed. Fifty percent of them had the formation of exudate and 62.5% of them showed delayed epithelial growth.</p><p><strong>Conclusion </strong>: This study could show hydrogen peroxide as wound antiseptic has delayed wound healing effect.</p><p><strong>Keyword</strong>: hydrogen peroxide, wound healing</p>

The Effect of a Hydrocolloid Dressing on Second Intention Wound Healing in Cats

2018 ◽  
Vol 54 (3) ◽  
pp. 125-131 ◽  
Author(s):  
Vassiliki Tsioli ◽  
Pagona G. Gouletsou ◽  
Apostolos D. Galatos ◽  
Dimitra Psalla ◽  
Antonios Lymperis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Evaluation ◽  
The Other ◽  
Skin Wounds ◽  
Tissue Formation ◽  
Dorsal Midline ◽  
Granulation Tissue Formation ◽  
Hydrocolloid Dressing ◽  
Thickness Skin ◽  
The Right

ABSTRACT The objective of the present study was to evaluate the effect of a hydrocolloid dressing on second intention wound healing in cats. Two full-thickness skin wounds, measuring 2 × 2 cm, were created on both sides of the dorsal midline of 10 cats; bilaterally, one randomly selected wound was bandaged with a hydrocolloid dressing and the second one (control) with a semiocclusive pad. Subjective clinical evaluation of granulation tissue formation, of the quantity and nature of wound exudate, and planimetry were performed on the right-side wounds, and histological examination on the left. No significant differences in subjective clinical evaluation or in planimetry were observed between the hydrocolloid-treated wounds and controls. Most wounds had serous or absence of exudate (41.25% and 25%, respectively), whereas purulent exudate was observed in 7.5% of wounds. Edema was significantly increased in the hydrocolloid-treated wounds compared with controls on day 7 but no significant differences in the other histological variables were observed.

scholarly journals Topical Application of Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) Accelerates Wound Healing in Diabetic Rabbits

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Abdulmohsen H. Al-Elq ◽  
Mir Sadat-Ali ◽  
Mohamed Elsharawy ◽  
Ibrahim Al-Habdan ◽  
Fatin Othman Al-Aqeel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessel ◽  
Topical Application ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Control Group ◽  
Collagen Deposition ◽  
Peptide Group ◽  
Diabetic Rabbits

Objective. Diminished wound healing is a common problem in diabetic patients due to diminished angiogenesis. SHMSP was found to promote angiogenesis. The present study was carried out to examine the effect of this peptide in healing of wounds in diabetic rabbits.Materials and Methods. Twenty male New Zealand rabbits were used in this study. Diabetes mellitus was induced and the rabbits were randomly divided into two equal groups: control group and peptide group. A-full thickness punch biopsy was made to create a wound of about 10 mm on the right ears of all rabbits. Every day, the wound was cleaned with saline in control groups. In the peptide group, 15 mg of SHMSP was applied after cleaning. On day 15th, all animals were sacrificed, and the wounds were excised with a rim of 5 mm of normal surrounding tissue. Histo-pathological assessment of wound healing, inflammatory cell infiltration, blood vessel proliferation, and collagen deposition was performed.Results. There were no deaths among the groups. There was significant increase in wound healing, blood vessel proliferation and collagen deposition, and significant decrease in inflammatory cell infiltration in the peptide group compared to the control group.Conclusion. Topical application of SHMSP improves wound healing in diabetic rabbits.

Topical application of complement C3 in collagen formulation increases early wound healing

2011 ◽  
Vol 24 (2) ◽  
pp. 141-147 ◽  
Author(s):  
Hani Sinno ◽  
Meenakshi Malholtra ◽  
Justyn Lutfy ◽  
Barbara Jardin ◽  
Sebastian Winocour ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Complement C3 ◽  
Early Wound

scholarly journals Collagen/Chitosan Gels Cross-Linked with Genipin for Wound Healing in Mice with Induced Diabetes

Materials ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 15
Author(s):  
Balzhima Shagdarova ◽  
Mariya Konovalova ◽  
Yuliya Zhuikova ◽  
Alexey Lunkov ◽  
Vsevolod Zhuikov ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Collagen Deposition ◽  
Sebaceous Glands ◽  
Hemostatic Effect ◽  
Potential Applications ◽  
Low Toxicity ◽  
Cd1 Mice ◽  
Diabetic Wounds ◽  
Timp1 Gene

Diabetes mellitus continues to be one of the most common diseases often associated with diabetic ulcers. Chitosan is an attractive biopolymer for wound healing due to its biodegradability, biocompatibility, mucoadhesiveness, low toxicity, and hemostatic effect. A panel of hydrogels based on chitosan, collagen, and silver nanoparticels were produced to treat diabetic wounds. The antibacterial activity, cytotoxicity, swelling, rheological properties, and longitudinal sections of hydrogels were studied. The ability of the gels for wound healing was studied in CD1 mice with alloxan-induced diabetes. Application of the gels resulted in an increase in VEGF, TGF-b1, IL-1b, and TIMP1 gene expression and earlier wound closure in a comparison with control untreated wounds. All gels increased collagen deposition, hair follicle repair, and sebaceous glands formation. The results of these tests show that the obtained hydrogels have good mechanical properties and biological activity and have potential applications in the field of wound healing. However, clinical studies are required to compare the efficacy of the gels as animal models do not reproduce full diabetes pathology.

scholarly journals Botrops derived hemocoagulase formulation a probable agent for diabetic wound healing

3 Biotech ◽  
2020 ◽  
Vol 10 (10) ◽  
Author(s):  
Raghuvir Keni ◽  
Karthik Gourishetti ◽  
Manas Kinra ◽  
Pawan G. Nayak ◽  
Rekha Shenoy ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Wound Closure ◽  
Punch Biopsy ◽  
Diabetic Rats ◽  
Dorsal Side ◽  
Collagen Deposition ◽  
Diabetic Wound ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds

Abstract Botroclot is a marketed preparation containing hemocoagulase, which is an enzyme having coagulant activity, isolated from the snake Botrops atrox. This formulation is used in dental surgeries and other minor surgical wounds. However, the formulation remains untested in diabetic wounds. Hence, we proposed a study for the topical application of Botroclot in high-fat diet (HFD) + Streptozotocin (STZ) induced diabetic rats. HFD was fed initially to rats which facilitates the development of insulin resistance. Thereafter, an injection of STZ (40 mg/kg, i.p.) was given. This resulted in the development of diabetes with elevated fasting glucose and impaired glucose tolerance. After stabilization of blood glucose values, wounds were created by punch biopsy on the dorsal side of the palm of the rat to mimic the diabetic wounds frequently seen in the case of humans. Later, the application of Botroclot on these wounds was carried out for 15 days. Topical application of hemocoagulase improved the wound closure and there was a gradual decrease in inflammatory markers and a substantial increase in collagen deposition occurred. Histopathological findings indicated the same, with an increase in granulation tissue suggesting that the topical application moderately improves the wound healing in diabetic rats. We conclude that Botroclot can have a mild to moderate effect in improving collagen deposition and thus wound contraction, improving wound closure in diabetic wounds in rats. This study also establishes the basis for exploration of agents from venom-based sources in diabetic wound healing.

scholarly journals The effect of hyaluronic acid on the skin healing in rats

2019 ◽  
Vol 10 (2) ◽  
pp. 65-75
Author(s):  
Livia Medeiros Soares Celani ◽  
Isadora Soares Lopes ◽  
Aldo Cunha Medeiros
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Granulation Tissue ◽  
Wound Repair ◽  
Histopathological Examination ◽  
Healing Process ◽  
Control Group ◽  
Skin Wounds ◽  
Collagen Deposition ◽  
Morphological Differences

Purpose: The objective of this study was to examine the effect of topical hyaluronic acid (HA) treatment on wound healing in rats. Methods: Wistar rats were randomly selected and allocated into 2 groups, with six rats each, all of them submitted to skin lesions (open wound with 5mm diameter). Group 1 – rats treated with topical 0,9% saline application in skin wounds. Group 2 – rats treated with topical hyaluronic acid 0,4% on skin wounds once a day for 7 days. After anesthesia on the 7th postoperative day, a biopsy of the skin dorsal wound area was performed for histopathological examination. A classification system based on the degree of reepithelization, granulation tissue formation and collagen organization were used to relate the morphological differences in wound healing. Results: Rats from control group treated with saline solution, on day 7, presented a general impairment of healing process, marked by an incomplete reepithelization (score 2,67 ± 0,4), a persistent exudation, fibrin, reduced accumulation of granulation tissue (score 2,2 ± 0,5) and a slight collagen deposition (score 2,43 ± 0,5), compared with the HA group. On the other side, morphological differences were visible on the evolution of wound repair process in rats treated with HA with a significant reduction in fibrinous exudation, epidermal lining reconstitution by complete reepithelization (score 3,8 ± 0,6), an increased accumulation of granulation tissue at wound edge and bed (score 3,7 ± 1,1), moderate collagen deposition (3,8 ± 0,7). The respective differences between the groups were significant (p<0,05). Conclusions: Based on these findings, we may conclude that HA treatment contributes to improve healing of open wounds.

scholarly journals A pulsatile release platform based on photo-induced imine-crosslinking hydrogel promotes scarless wound healing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jian Zhang ◽  
Yongjun Zheng ◽  
Jimmy Lee ◽  
Jieyu Hua ◽  
Shilong Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Skin Wound ◽  
Large Animal ◽  
Skin Wounds ◽  
Tgfβ Signaling ◽  
Pulsatile Release ◽  
Ecm Extracellular Matrix

AbstractEffective healing of skin wounds is essential for our survival. Although skin has strong regenerative potential, dysfunctional and disfiguring scars can result from aberrant wound repair. Skin scarring involves excessive deposition and misalignment of ECM (extracellular matrix), increased cellularity, and chronic inflammation. Transforming growth factor-β (TGFβ) signaling exerts pleiotropic effects on wound healing by regulating cell proliferation, migration, ECM production, and the immune response. Although blocking TGFβ signaling can reduce tissue fibrosis and scarring, systemic inhibition of TGFβ can lead to significant side effects and inhibit wound re-epithelization. In this study, we develop a wound dressing material based on an integrated photo-crosslinking strategy and a microcapsule platform with pulsatile release of TGF-β inhibitor to achieve spatiotemporal specificity for skin wounds. The material enhances skin wound closure while effectively suppressing scar formation in murine skin wounds and large animal preclinical models. Our study presents a strategy for scarless wound repair.

scholarly journals Systematic Review: Adipose-Derived Mesenchymal Stem Cells, Platelet-Rich Plasma and Biomaterials as New Regenerative Strategies in Chronic Skin Wounds and Soft Tissue Defects

2021 ◽  
Vol 22 (4) ◽  
pp. 1538 ◽  
Author(s):  
Pietro Gentile ◽  
Simone Garcovich
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Wound Healing ◽  
Soft Tissue ◽  
Chronic Wounds ◽  
Platelet Rich Plasma ◽  
Skin Wounds ◽  
Soft Tissue Defects ◽  
Chronic Skin ◽  
Tissue Defects

The number of clinical trials evaluating adipose-derived mesenchymal stem cells (AD-MSCs), platelet-rich plasma (PRP), and biomaterials efficacy in regenerative plastic surgery has exponentially increased during the last ten years. AD-MSCs are easily accessible from various fat depots and show intrinsic plasticity in giving rise to cell types involved in wound healing and angiogenesis. AD-MSCs have been used in the treatment of soft tissue defects and chronic wounds, employed in conjunction with a fat grafting technique or with dermal substitute scaffolds and platelet-rich plasma. In this systematic review, an overview of the current knowledge on this topic has been provided, based on existing studies and the authors’ experience. A multistep search of the PubMed, MEDLINE, Embase, PreMEDLINE, Ebase, CINAHL, PsycINFO, Clinicaltrials.gov, Scopus database, and Cochrane databases has been performed to identify papers on AD-MSCs, PRP, and biomaterials used in soft tissue defects and chronic wounds. Of the 2136 articles initially identified, 422 articles focusing on regenerative strategies in wound healing were selected and, consequently, only 278 articles apparently related to AD-MSC, PRP, and biomaterials were initially assessed for eligibility. Of these, 85 articles were excluded as pre-clinical, experimental, and in vitro studies. For the above-mentioned reasons, 193 articles were selected; of this amount, 121 letters, expert opinions, commentary, and editorials were removed. The remaining 72 articles, strictly regarding the use of AD-MSCs, PRP, and biomaterials in chronic skin wounds and soft tissue defects, were analyzed. The studies included had to match predetermined criteria according to the patients, intervention, comparator, outcomes, and study design (PICOS) approach. The information analyzed highlights the safety and efficacy of AD-MSCs, PRP, and biomaterials on soft tissue defects and chronic wounds, without major side effects.

Sign in / Sign up

Export Citation Format

Share Document