scholarly journals Potency of Fish Collagen as a Scaffold for Regenerative Medicine

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Shizuka Yamada ◽  
Kohei Yamamoto ◽  
Takeshi Ikeda ◽  
Kajiro Yanagiguchi ◽  
Yoshihiko Hayashi
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Regenerative Medicine ◽  
Collagen Molecule ◽  
Specific Cell ◽  
Natural Polymers ◽  
Collagen Scaffolds ◽  
Rgd Motif ◽  
Cell Functions ◽  
Fish Collagen

Cells, growth factors, and scaffold are the crucial factors for tissue engineering. Recently, scaffolds consisting of natural polymers, such as collagen and gelatin, bioabsorbable synthetic polymers, such as polylactic acid and polyglycolic acid, and inorganic materials, such as hydroxyapatite, as well as composite materials have been rapidly developed. In particular, collagen is the most promising material for tissue engineering due to its biocompatibility and biodegradability. Collagen contains specific cell adhesion domains, including the arginine-glycine-aspartic acid (RGD) motif. After the integrin receptor on the cell surface binds to the RGD motif on the collagen molecule, cell adhesion is actively induced. This interaction contributes to the promotion of cell growth and differentiation and the regulation of various cell functions. However, it is difficult to use a pure collagen scaffold as a tissue engineering material due to its low mechanical strength. In order to make up for this disadvantage, collagen scaffolds are often modified using a cross-linker, such as gamma irradiation and carbodiimide. Taking into account the possibility of zoonosis, a variety of recent reports have been documented using fish collagen scaffolds. We herein review the potency of fish collagen scaffolds as well as associated problems to be addressed for use in regenerative medicine.

Recent Advances in Biohybrid Materials for Tissue Engineering and Regenerative Medicine

2016 ◽  
Vol 04 (01) ◽  
pp. 1640001 ◽  
Author(s):  
Ying Wan ◽  
Xing Li ◽  
Shenqi Wang
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cell Function ◽  
Rapid Prototype ◽  
Artificial Organs ◽  
Specific Cell ◽  
Cell Matrix ◽  
Matrix Surface ◽  
The Matrix ◽  
Cell Matrix Interactions

Biohybrid materials play an important role in tissue engineering, artificial organs and regenerative medicine due to their regulation of cell function through specific cell–matrix interactions involving integrins, mostly those of fibroblasts and myofibroblasts, and ligands on the matrix surface, which have become current research focus. In this paper, recent progress of biohybrid materials, mainly including main types of biohybrid materials, rapid prototype (RP) technique for construction of 3D biohybrid materials, was reviewed in detail; moreover, their applications in tissue engineering, artificial organs and regenerative medicine were also reviewed in detail. At last, we address the challenges biohybrid materials may face.

Cell colonization of scaffolds for tissue engineering enhanced by means of plasma processes

2012 ◽  
Vol 1469 ◽  
Author(s):  
P. Favia ◽  
E. Sardella ◽  
R.A.H. Salama ◽  
V. R. Giampietro ◽  
F. Intranuovo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Plasma Deposition ◽  
Porous Polymer ◽  
Polymer Scaffolds ◽  
Natural Polymers ◽  
Biodegradable Scaffolds ◽  
Ft Ir ◽  
Cell Colonization

ABSTRACTSynthetic biodegradable polymers are commonly used as scaffolds for tissue engineering despite their poor cell adhesion compared to natural polymers. One of the problems in using biodegradable scaffolds is that a higher cell colonization at the scaffold periphery and inadequate colonization at its center is generally noted. Such aspects could seriously compromise the in vivo regeneration of a damaged tissue and, in turn, the success of the implant. Plasma processes have been lately proven as promising scaffold modification techniques. The current work aims at enhancing cell colonization in the core of polymer scaffolds via plasma deposition of coatings with different chemical characteristics. The versatility and ability of plasma processes to modify only the outermost layer of a material can render them competitive with respect to wet chemistry approaches in the field of biomedical materials. In this paper some of the results obtained by plasma processing of 3D interconnected porous polymer scaffolds for Tissue Engineering will be shown. In particular, it will be shown how it is possible to enhance cell adhesion, growth and colonization in porous Polycaprolactone (PCL) scaffolds where gradient of surface compositions are induced from the external (e.g., hydrophobic, slightly cell-repulsive) to the internal (e.g., hydrophilic, cell-adhesive) side of the scaffolds. 3D scaffolds were modified with several RF (13.56 MHz) deposition and treatment plasma processes. Materials were characterized by means of XPS, and FT-IR techniques. Cell-growth experiments were run with cell-lines to check the efficiency of several treatments to enhance/accelerate cell in-growth inside scaffolds.

Engineered Biomimetic Nanofibers for Regenerative Medicine

2010 ◽  
Vol 76 ◽  
pp. 114-124
Author(s):  
Seeram Ramakrishna ◽  
Jayarama Reddy Venugopal ◽  
Susan Liao
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Cost Effective ◽  
Bioactive Molecules ◽  
Natural Polymers ◽  
Hematopoietic Stem

Attempts have been made to fabricate nanofibrous scaffolds to mimic the chemical composition and structural properties of extracellular matrix (ECM) for tissue/organ regeneration. Nanofibers with various patterns have been successfully produced from synthetic and natural polymers through a relatively simple technique of electrospinning. The resulting patterns can mimic some of the diverse tissue-specific orientation and three-dimensional (3D) fibrous structure. Studies on cell-nanofiber interactions have revealed the importance of nanotopography on cell adhesion, proliferation and differentiation. Our recent data showed that hematopoietic stem cells (HSCs) as well as mesenchymal stem cells (MSCs) can rapidly and effectively attached to the functionalized nanofibers. Mineralized 3D nanofibrous scaffold with bone marrow derived MSCs has been applied for bone tissue engineering. The use of injectable nanofibers for cardiac tissue engineering applications is attractive as they allow for the encapsulation of cardiomyocytes/MSCs as well as bioactive molecules for the repair of myocardial infarction. Duplicate 3D heart helix microstructure by the nanofibrous cardiac patch might provide functional support for infarcted myocardium. Furthermore, clinical applications of electrospun nanofibers for regenerative medicine are highly feasible due to the ease and flexibility of fabrication with the cost-effective method of making nanofibers.

scholarly journals A State-of-the-Art of Functional Scaffolds for 3D Nervous Tissue Regeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Maria Grazia Tupone ◽  
Michele d’Angelo ◽  
Vanessa Castelli ◽  
Mariano Catanesi ◽  
Elisabetta Benedetti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Tissue Regeneration ◽  
Nervous Tissue ◽  
Functional Materials ◽  
Molecular Techniques ◽  
Cell Phenotype ◽  
Specific Cell

Exploring and developing multifunctional intelligent biomaterials is crucial to improve next-generation therapies in tissue engineering and regenerative medicine. Recent findings show how distinct characteristics of in situ microenvironment can be mimicked by using different biomaterials. In vivo tissue architecture is characterized by the interconnection between cells and specific components of the extracellular matrix (ECM). Last evidence shows the importance of the structure and composition of the ECM in the development of cellular and molecular techniques, to achieve the best biodegradable and bioactive biomaterial compatible to human physiology. Such biomaterials provide specialized bioactive signals to regulate the surrounding biological habitat, through the progression of wound healing and biomaterial integration. The connection between stem cells and biomaterials stimulate the occurrence of specific modifications in terms of cell properties and fate, influencing then processes such as self-renewal, cell adhesion and differentiation. Recent studies in the field of tissue engineering and regenerative medicine have shown to deal with a broad area of applications, offering the most efficient and suitable strategies to neural repair and regeneration, drawing attention towards the potential use of biomaterials as 3D tools for in vitro neurodevelopment of tissue models, both in physiological and pathological conditions. In this direction, there are several tools supporting cell regeneration, which associate cytokines and other soluble factors delivery through the scaffold, and different approaches considering the features of the biomaterials, for an increased functionalization of the scaffold and for a better promotion of neural proliferation and cells-ECM interplay. In fact, 3D scaffolds need to ensure a progressive and regular delivery of cytokines, growth factors, or biomolecules, and moreover they should serve as a guide and support for injured tissues. It is also possible to create scaffolds with different layers, each one possessing different physical and biochemical aspects, able to provide at the same time organization, support and maintenance of the specific cell phenotype and diversified ECM morphogenesis. Our review summarizes the most recent advancements in functional materials, which are crucial to achieve the best performance and at the same time, to overcome the current limitations in tissue engineering and nervous tissue regeneration.

Applications of Poly (caprolactone)-Based Nanofibre Electrospun Scaffolds in Tissue Engineering and Regenerative Medicine

2020 ◽  
Vol 15 ◽  
Author(s):  
Wei Zhang ◽  
Tingting Weng ◽  
Qiong Li ◽  
Ronghua Jin ◽  
Chuangang You ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Biological Properties ◽  
Future Research ◽  
Natural Polymers ◽  
Bioactive Substances ◽  
Electrospun Scaffolds ◽  
Recent Developments ◽  
Poly Caprolactone

: Diseases, trauma, and injuries are highly prevalent conditions that lead to many critical tissue defects. Tissue engineering has great potentials to develop functional scaffolds that mimic natural tissue structures to improve or replace biological functions. In many kinds of technologies, electrospinning has received widespread attention for its outstanding functions, which is capable of producing nanofibre structures similar to the natural extracellular matrix. Amongst, the electrospinning of available biopolymers, poly (caprolactone) (PCL), has shown favorable outcomes for tissue regeneration applications. According to the characteristics of different tissues, PCL can be modified by altering the functional groups or combining with other materials such as synthetic polymers, natural polymers, and metal materials to improve its physicochemical, mechanical, and biological properties, making the electrospun scaffolds meet the requirements of different tissue engineering and regenerative medicine. Moreover, efforts have been made to modify nanofibres with several bioactive substances to provide cells with the necessary chemical cues and a more in vivo like environment. In this review, some recent developments in both the design and utility of electrospun PCL-based scaffolds in the fields of bone, cartilage, skin, tendon, ligament and nerve are highlighted, along with their potential impact on future research and clinical applications.

scholarly journals Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

Membranes ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 348
Author(s):  
Monika Wasyłeczko ◽  
Wioleta Sikorska ◽  
Andrzej Chwojnowski
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Hybrid Materials ◽  
Cartilage Tissue Engineering ◽  
Biological Properties ◽  
General Information ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Natural Polymers ◽  
Cellular Environment

Cartilage tissue is under extensive investigation in tissue engineering and regenerative medicine studies because of its limited regenerative potential. Currently, many scaffolds are undergoing scientific and clinical research. A key for appropriate scaffolding is the assurance of a temporary cellular environment that allows the cells to function as in native tissue. These scaffolds should meet the relevant requirements, including appropriate architecture and physicochemical and biological properties. This is necessary for proper cell growth, which is associated with the adequate regeneration of cartilage. This paper presents a review of the development of scaffolds from synthetic polymers and hybrid materials employed for the engineering of cartilage tissue and regenerative medicine. Initially, general information on articular cartilage and an overview of the clinical strategies for the treatment of cartilage defects are presented. Then, the requirements for scaffolds in regenerative medicine, materials intended for membranes, and methods for obtaining them are briefly described. We also describe the hybrid materials that combine the advantages of both synthetic and natural polymers, which provide better properties for the scaffold. The last part of the article is focused on scaffolds in cartilage tissue engineering that have been confirmed by undergoing preclinical and clinical tests.

scholarly journals Prevascularized Micro-/Nano-Sized Spheroid/Bead Aggregates for Vascular Tissue Engineering

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Maedeh Rahimnejad ◽  
Narges Nasrollahi Boroujeni ◽  
Sepideh Jahangiri ◽  
Navid Rabiee ◽  
Mohammad Rabiee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cardiovascular System ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Specific Cell ◽  
Microvascular Network ◽  
Cell Components ◽  
Microvascular Engineering

AbstractEfficient strategies to promote microvascularization in vascular tissue engineering, a central priority in regenerative medicine, are still scarce; nano- and micro-sized aggregates and spheres or beads harboring primitive microvascular beds are promising methods in vascular tissue engineering. Capillaries are the smallest type and in numerous blood vessels, which are distributed densely in cardiovascular system. To mimic this microvascular network, specific cell components and proangiogenic factors are required. Herein, advanced biofabrication methods in microvascular engineering, including extrusion-based and droplet-based bioprinting, Kenzan, and biogripper approaches, are deliberated with emphasis on the newest works in prevascular nano- and micro-sized aggregates and microspheres/microbeads.

scholarly journals Biomaterials in Regenerative Medicine

2012 ◽  
Vol 46 (2) ◽  
pp. 81-89 ◽  
Author(s):  
Sumrita Bhat ◽  
Ashok Kumar
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Orthopedic Implants ◽  
Specific Cell ◽  
Initial Growth ◽  
Porous Network ◽  
Recent Advances ◽  
Conventional Methods

ABSTRACT Limitations with the conventional methods have bought biomaterials to the forefront for the repair and restoration of tissue functions. Recent advances in the area of biomaterials have revolutionized the field of tissue engineering and regenerative medicine. According to the nature of polymers they are divided into different classes and each one has found applicability in the area of regenerative medicine. Each class of biomaterials has a set of properties which makes them appropriate for a specific application. The most important property is the behavior of biomaterials when implanted in vivo. It should not elicit any immune rejection reactions neither should its byproducts be toxic to animal tissue. Any type of the biomaterial can be fabricated into a three-dimensional scaffold which can be used as housing for the initial growth and proliferation of the specific cell type. In addition to the conventional methods of scaffold fabrication few contemporary methods include ‘hydrogels’ and ‘cryogels’. These matrices possess interconnected porous network which facilitates the cell migration and proliferation. These gel matrices can be fabricated from both natural and synthetic polymers and have shown applicability in different areas of tissue engineering. Biomaterials have shown applicability as cardiovascular implants, orthopedic implants, dental implants, etc. Furthermore, recent advances in the regenerative medicine have shown that in addition to the use of autologous and allogenic sources, stem cells can prove to be a very good alternative. Stem cells interaction with biomaterials has shown applicability in the regenerative medicine and thus can have an immense potential in future. How to cite this article Bhat S, Kumar A. Biomaterials in Regenerative Medicine. J Postgrad Med Edu Res 2012;46(2):81-89.

scholarly journals Recent Advances in Natural Gum-Based Biomaterials for Tissue Engineering and Regenerative Medicine: A Review

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 176 ◽  
Author(s):  
Reza Mohammadinejad ◽  
Anuj Kumar ◽  
Marziyeh Ranjbar-Mohammadi ◽  
Milad Ashrafizadeh ◽  
Sung Soo Han ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Biological Response ◽  
3D Cell Culture ◽  
Skin Wound ◽  
Natural Polymers ◽  
3D Microenvironment ◽  
Artificial Extracellular Matrix

The engineering of tissues under a three-dimensional (3D) microenvironment is a great challenge and needs a suitable supporting biomaterial-based scaffold that may facilitate cell attachment, spreading, proliferation, migration, and differentiation for proper tissue regeneration or organ reconstruction. Polysaccharides as natural polymers promise great potential in the preparation of a three-dimensional artificial extracellular matrix (ECM) (i.e., hydrogel) via various processing methods and conditions. Natural polymers, especially gums, based upon hydrogel systems, provide similarities largely with the native ECM and excellent biological response. Here, we review the origin and physico-chemical characteristics of potentially used natural gums. In addition, various forms of scaffolds (e.g., nanofibrous, 3D printed-constructs) based on gums and their efficacy in 3D cell culture and various tissue regenerations such as bone, osteoarthritis and cartilage, skin/wound, retinal, neural, and other tissues are discussed. Finally, the advantages and limitations of natural gums are precisely described for future perspectives in tissue engineering and regenerative medicine in the concluding remarks.

scholarly journals Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Martin Rodríguez-Vázquez ◽  
Brenda Vega-Ruiz ◽  
Rodrigo Ramos-Zúñiga ◽  
Daniel Alexander Saldaña-Koppel ◽  
Luis Fernando Quiñones-Olvera
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Biomedical Applications ◽  
Therapeutic Strategy ◽  
Biological Properties ◽  
Natural Polymers ◽  
Tissue Engineering Scaffolds ◽  
Promising Alternative ◽  
Functional Biomaterials ◽  
Potential Use

Tissue engineering is an important therapeutic strategy to be used in regenerative medicine in the present and in the future. Functional biomaterials research is focused on the development and improvement of scaffolding, which can be used to repair or regenerate an organ or tissue. Scaffolds are one of the crucial factors for tissue engineering. Scaffolds consisting of natural polymers have recently been developed more quickly and have gained more popularity. These include chitosan, a copolymer derived from the alkaline deacetylation of chitin. Expectations for use of these scaffolds are increasing as the knowledge regarding their chemical and biological properties expands, and new biomedical applications are investigated. Due to their different biological properties such as being biocompatible, biodegradable, and bioactive, they have given the pattern for use in tissue engineering for repair and/or regeneration of different tissues including skin, bone, cartilage, nerves, liver, and muscle. In this review, we focus on the intrinsic properties offered by chitosan and its use in tissue engineering, considering it as a promising alternative for regenerative medicine as a bioactive polymer.

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