scholarly journals A Metabolic Study on Colon Cancer Using1H Nuclear Magnetic Resonance Spectroscopy

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Zahra Zamani ◽  
Mohammad Arjmand ◽  
Farideh Vahabi ◽  
Seyed Mahmood Eshaq Hosseini ◽  
Sadegh Mohammad Fazeli ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Metabolic Pathways ◽  
Vitamin B6 ◽  
Screening Tests ◽  
Glutathione Metabolism ◽  
Resonance Spectroscopy ◽  
Metabolic Study ◽  
Acid Biosynthesis ◽  
Noninvasive Test ◽  
Methane Metabolism

Background. Colorectal carcinoma is the third cause of cancer deaths in the world. For diagnosis, invasive methods like colonoscopy and sigmoidoscopy are used, and noninvasive screening tests are not very accurate. We decided to study the potential of1HNMR spectroscopy with metabolomics and chemometrics as a preliminary noninvasive test. We obtained a distinguishing pattern of metabolites and metabolic pathways between colon cancer patient and normal.Methods. Sera were obtained from confirmed colon cancer patients and the same number of healthy controls. Samples were sent for1HNMR spectroscopy and analysis was carried out Chenomex and MATLAB software. Metabolites were identified using Human Metabolic Data Base (HDMB) and the main metabolic cycles were identified using Metaboanalyst software.Results. 15 metabolites were identified such as pyridoxine, orotidine, and taurocholic acid. Main metabolic cycles involved were the bile acid biosynthesis, vitamin B6 metabolism, methane metabolism, and glutathione metabolism.Discussion. The main detected metabolic cycles were also reported earlier in different cancers. Our observations corroborated earlier studies that suggest the importance of lowering serum LCA/DCA and increasing vitamin B6 intake to help prevent colon cancer. This work can be looked upon as a preliminary step in using1HNMR analysis as a screening test before invasive procedures.

scholarly journals Metabolomics reveals disturbed metabolic pathways in human lung epithelial cells exposed to airborne fine particulate matter

2015 ◽  
Vol 4 (4) ◽  
pp. 939-947 ◽  
Author(s):  
Qingyu Huang ◽  
Jie Zhang ◽  
Lianzhong Luo ◽  
Xiaofei Wang ◽  
Xiaoxue Wang ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Human Lung ◽  
Fine Particulate Matter ◽  
A549 Cells ◽  
Lung Epithelial Cells ◽  
Glutathione Metabolism ◽  
Acid Biosynthesis ◽  
Citrate Cycle ◽  
Fine Particulate ◽  
Lung Epithelial

Airborne PM2.5 exposure disturbs citrate cycle, amino acid biosynthesis and metabolism, and glutathione metabolism in A549 cells.

Neurometabolic Diseases in Children: Magnetic Resonance Imaging and Magnetic Resonance Spectroscopy Features

2019 ◽  
Vol 15 (3) ◽  
pp. 255-268
Author(s):  
Direnç Özlem Aksoy ◽  
Alpay Alkan
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Metabolic Pathways ◽  
Wide Spectrum ◽  
Demyelinating Diseases ◽  
Resonance Spectroscopy ◽  
Resonance Imaging ◽  
Neurometabolic Disorders ◽  
Brain Involvement ◽  
The Brain

Background: Neurometabolic diseases are a group of diseases secondary to disorders in different metabolic pathways, which lead to white and/or gray matter of the brain involvement. </P><P> Discussion: Neurometabolic disorders are divided in two groups as dysmyelinating and demyelinating diseases. Because of wide spectrum of these disorders, there are many different classifications of neurometabolic diseases. We used the classification according to brain involvement areas. In radiological evaluation, MRI provides useful information for these disseases. Conclusion: Magnetic Resonance Spectroscopy (MRS) provides additional metabolic information for diagnosis and follow ups in childhood with neurometabolic diseases.

scholarly journals A Nuclear Magnetic Resonance Spectroscopy-based Approach to Profile Biologically Active Plant Metabolites Using Black Raspberry Inhibition of Colon Cancer Cell Proliferation as a Model System

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1000B-1000
Author(s):  
Faith J. Wyzgoski ◽  
A. Raymond Miller ◽  
Joseph C. Scheerens ◽  
Peter L. Rinaldi ◽  
Bert L. Bishop ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Biologically Active ◽  
Resonance Spectroscopy ◽  
Black Raspberry ◽  
Bioactive Components ◽  
Plant Metabolites ◽  
Black Raspberries ◽  
Nuclear Magnetic

We have developed a Nuclear Magnetic Resonance (NMR)-based approach to metabolomics research that enables the identification of bioactive compounds in crude plant extracts. For this work, we used black raspberries, which are known to contain compounds that exhibit chemopreventive activity toward oral, esophageal, and colon cancers. To ascertain bioactive components and their interrelationships, NMR results for black raspberry samples from four cultivars grown on commercial farms in Ohio were examined using principal component analysis. Multivariate analysis that included anthocyanin content (HPLC), antioxidant activity (DPPH, ABTS, FRAP), total phenolics (Folin-Ciocalteau assay), and bioactivity as measured by inhibition of colon cancer HT-29 cell line proliferation showed correlations with specific regions of NMR spectra at 400 MHz. Correlations were also observed for major and minor groupings of the black raspberry samples. Replicate black raspberry samples were examined with a 750 MHz NMR spectrometer equipped with a cryoprobe that provided a 4- to 5-fold improvement in sensitivity. In this manner, even minor bioactive components in black raspberries could be examined to determine additive and synergistic effects.

scholarly journals George Joseph Popjàk. 5 May 1914 — 30 December 1998

2000 ◽  
Vol 46 ◽  
pp. 403-424
Author(s):  
Muhammad Akhtar
Keyword(s):  
Metabolic Pathways ◽  
Fatty Acid Biosynthesis ◽  
Medical Training ◽  
Cholesterol Biosynthesis ◽  
Mevalonic Acid ◽  
Cholesterol Homeostasis ◽  
Joint Work ◽  
Acid Biosynthesis ◽  
Physiological Conditions ◽  
The Uk

George Joseph Popják was a leading biochemist who introduced radioisotopic techniques to the study of metabolic pathways in the UK, and fully exploited their use in his own researches. His earlier work clarified the origin of triglycerides and cholesterol in the foetus as well as in milk, and paved the way for far-reaching discoveries in these fields. His group was the first to demonstrate that fatty acid biosynthesis occurs not in the mitochondria by the mere reversal of the β-oxidation pathway, but by a new cytosolic enzyme system. With J.W. Cornforth he dominated the field of cholesterol biosynthesis for nearly two decades. Their joint work reached new heights when they made intellectually cunning contributions to the mechanism and stereochemistry of several enzymic reactions involved between mevalonic acid and squalene. His later work was concerned with the understanding of the factors that, under physiological conditions, maintain cholesterol homeostasis and the development of novel strategies that can be used in the treatment of hypercholesterolaemia. Popják's early medical training and his great mastery of chemical enzymology provided a powerful combination for tackling biomedically important problems at a molecular level.

scholarly journals Dietary Folic Acid and Fat Alter Metabolism of Multiple Vitamins in Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1778-1778
Author(s):  
Keri Barron ◽  
Natalia Krupenko
Keyword(s):  
Folic Acid ◽  
Vitamin A ◽  
Metabolic Pathways ◽  
Vitamin B6 ◽  
High Fat ◽  
Low Fat ◽  
Funding Sources ◽  
Low Fat Diet ◽  
High Fat Diets ◽  
Fat Diets

Abstract Objectives To determine how low and high folic acid (FA) intake, combined with either low or high-fat diets, affects other vitamins in mouse liver and plasma. Methods C57BL/6NHsd mice were placed on one of six diets at weaning and maintained for 16 weeks. The diets varied in their fat content and FA levels: low fat (14% kcal from fat) vs high fat (58% kcal from fat) with 3 different FA levels- 0 ppm FA (FD), 2 ppm FA (Ctrl), 12 ppm (FS). Diets were matched for all other vitamins and minerals. Untargeted metabolomics analysis of plasma and snap-frozen liver samples was conducted at Metabolon®. Results In liver, excess dietary folic acid on a low-fat diet resulted in significantly increased levels of pantothenate, α-tocopherol, FA and several folate metabolites. When FA was over-supplemented in combination with a high fat (HF) diet, α-tocopherol was increased along with several nicotinate and pantothenate metabolites. Interestingly, the HF-FD and -FS diets demonstrated similar effects. These diets resulted in significantly decreased levels of riboflavin, thiamine, vitamin A, and vitamin B6 metabolites while increasing levels of pantetheine metabolites. In plasma, fewer changes with significant differences were observed when mice were fed HF diets. Several nicotinate metabolites were significantly elevated due to the FD diet with no change due to FS. Additionally, there were no changes in pantothenate or riboflavin in the plasma. Interestingly, the HF- FD and -FS diets induced similar responses but in opposite directions in plasma vs liver. The plasma levels of thiamine, vitamin A, and vitamin B6 metabolites were all significantly increased due to both low and high FA, whereas in the liver they were decreased. Additionally, no changes in α-tocopherol were seen in plasma, but the HF-FD diet raised γ/β-tocopherol levels over 2-fold despite equal amounts of vitamin E among all diets. Conclusions Untargeted metabolomic analysis revealed that diets with too high or too low folate affect other vitamins both in liver and plasma. These effects were further modulated by dietary fat levels. The HF-FD and -FS diets had significant impact on vitamins A, B1, B2, B3, B5, B6, B9 and E, along with their related derivatives, which may have serious implications for multiple metabolic pathways. Funding Sources NIH.

scholarly journals Changes in hepatic metabolic profile during the evolution of STZ-induced diabetic rats via an 1H NMR-based metabonomic investigation

2019 ◽  
Vol 39 (4) ◽  
Author(s):  
Minjiang Chen ◽  
Hong Zheng ◽  
Min Xu ◽  
Liangcai Zhao ◽  
Qianqian Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
1H Nmr ◽  
Metabolic Pathways ◽  
Metabolic Profile ◽  
Diabetic Rats ◽  
Proton Nuclear Magnetic Resonance ◽  
Control Group ◽  
Resonance Spectroscopy ◽  
Discriminate Analysis ◽  
Protective Function

Abstract Background: The present study aimed to explore the changes in the hepatic metabolic profile during the evolution of diabetes mellitus (DM) and verify the key metabolic pathways. Methods: Liver samples were collected from diabetic rats induced by streptozotocin (STZ) and rats in the control group at 1, 5, and 9 weeks after STZ administration. Proton nuclear magnetic resonance spectroscopy (1H NMR)-based metabolomics was used to examine the metabolic changes during the evolution of DM, and partial least squares-discriminate analysis (PLS-DA) was performed to identify the key metabolites. Results: We identified 40 metabolites in the 1H NMR spectra, and 11 metabolites were further selected by PLS-DA model. The levels of α-glucose and β-glucose, which are two energy-related metabolites, gradually increased over time in the DM rats, and were significantly greater than those of the control rats at the three-time points. The levels of choline, betaine, and methionine decreased in the DM livers, indicating that the protective function in response to liver injury may be undermined by hyperglycemia. The levels of the other amino acids (leucine, alanine, glycine, tyrosine, and phenylalanine) were significantly less than those of the control group during DM development. Conclusions: Our results suggested that the hepatic metabolic pathways of glucose, choline-betaine-methionine, and amino acids were disturbed during the evolution of diabetes, and that choline-betaine-methionine metabolism may play a key role.

scholarly journals Changes in Metabolites from Bovine Milk with β-Casein Variants Revealed by Metabolomics

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 954
Author(s):  
Zhongwang Lv ◽  
Hui Liu ◽  
Yongxin Yang ◽  
Dengpan Bu ◽  
Changjiang Zang ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
High Resolution Melting ◽  
Bovine Milk ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Resonance Spectroscopy ◽  
Liquid Chromatography Mass Spectrometry ◽  
Multivariate Statistical ◽  
Isoleucine Biosynthesis ◽  
Pathways Analysis

β-casein is a primary protein in milk, and its variants have been associated with changes in the protein content of bovine milk. However, there has been little research focused on the effects of β-casein variants on milk metabolites. In the present study, dairy cows producing milk with β-casein variant A1/A1 (A1), A2/A2 (A2), and their heterozygote A1/A2 (A12) were screened by a high-resolution melting method. Individual milk samples were then collected from each of the cows, and the milk metabolites were separated and analyzed using nuclear magnetic resonance spectroscopy- and liquid-chromatography mass spectrometry-based metabolomics techniques. Differences in metabolites among the variant groups were evaluated by multivariate statistical analysis. The relative abundances of methionine, proline, and α-lactose were the highest in β-casein variant A2 milk, whereas choline, glycine, citric acid, and cyclic adenosine monophosphate (cAMP) showed the highest abundances in variant A1 milk. Metabolic pathways analysis indicated that the differential metabolites between variants A1 and A2 were involved in pantothenate and coenzyme A biosynthesis, butanoate metabolism, and valine, leucine, and isoleucine biosynthesis. Our results reveal the differences in milk metabolites among the β-casein variants A1, A2, and the heterozygote. These findings, thus, provide novel insights into the effects of β-casein variants on milk metabolites, facilitating further research into the mechanism of the biosynthesis of milk components in the mammary gland and the potential physiological function of milk associated with β-casein variants.

scholarly journals Evaluation of an aldo-keto reductase gene signature with prognostic significance in colon cancer via activation of epithelial to mesenchymal transition and the p70S6K pathway

2020 ◽  
Vol 41 (9) ◽  
pp. 1219-1228
Author(s):  
Seçil Demirkol Canlı ◽  
Esin Gülce Seza ◽  
Ilir Sheraj ◽  
Ismail Gömçeli ◽  
Nesrin Turhan ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Metabolic Pathways ◽  
Epithelial To Mesenchymal Transition ◽  
Ex Vivo ◽  
Prognostic Significance ◽  
Distal Colon ◽  
Polyol Pathway ◽  
Gene Signature ◽  
Nutrient Sensing ◽  
Mesenchymal Transition

Abstract AKR1B1 and AKR1B10, members of the aldo-keto reductase family of enzymes that participate in the polyol pathway of aldehyde metabolism, are aberrantly expressed in colon cancer. We previously showed that high expression of AKR1B1 (AKR1B1HIGH) was associated with enhanced motility, inflammation and poor clinical outcome in colon cancer patients. Using publicly available datasets and ex vivo gene expression analysis (n = 51, Ankara cohort), we have validated our previous in silico finding that AKR1B1HIGH was associated with worse overall survival (OS) compared with patients with low expression of AKR1B1 (AKR1B1LOW) samples. A combined signature of AKR1B1HIGH and AKR1B10LOW was significantly associated with worse recurrence-free survival (RFS) in microsatellite stable (MSS) patients and in patients with distal colon tumors as well as a higher mesenchymal signature when compared with AKR1B1LOW/AKR1B10HIGH tumors. When the patients were stratified according to consensus molecular subtypes (CMS), AKR1B1HIGH/AKR1B10LOW samples were primarily classified as CMS4 with predominantly mesenchymal characteristics while AKR1B1LOW/AKR1B10HIGH samples were primarily classified as CMS3 which is associated with metabolic deregulation. Reverse Phase Protein Array carried out using protein samples from the Ankara cohort indicated that AKR1B1HIGH/AKR1B10LOW tumors showed aberrant activation of metabolic pathways. Western blot analysis of AKR1B1HIGH/AKR1B10LOW colon cancer cell lines also suggested aberrant activation of nutrient-sensing pathways. Collectively, our data suggest that the AKR1B1HIGH/AKR1B10LOW signature may be predictive of poor prognosis, aberrant activation of metabolic pathways, and can be considered as a novel biomarker for colon cancer prognostication.

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