scholarly journals Controlling of oral pathogens using ginger oleoresin mediated silver nanoparticles

2019 ◽  
Vol 10 (4) ◽  
pp. 2988-2991 ◽  
Author(s):  
Karthikeyan Gayathri ◽  
Anitha Roy ◽  
Thangavelu Lakshmi ◽  
Rajeshkumar S
Keyword(s):  
Silver Nanoparticles ◽  
Low Cost ◽  
Diffusion Method ◽  
Natural Substances ◽  
Antimicrobial Sensitivity ◽  
Vis Spectroscopy ◽  
Wide Range ◽  
Test Organisms ◽  
Oral Pathogens ◽  
Agar Well Diffusion Method

Silver nanoparticles have become a substance of great interest among researches in the present time due to its low cost and its wide range of applications in various fields. Moreover, recently, green nanotechnology has been gaining popularity because of its exclusion of damaging reagents and cost-effectiveness. In this study, ginger oleoresin mediated silver nanoparticles were prepared and confirmed with UV-Vis spectroscopy. The ginger oleoresin mediated silver nanoparticles were then tested for its antibacterial activity using agar well diffusion method against Staphylococcus aureus, Streptococcus mutans, and Enterococcus faecalis. The antimicrobial sensitivity was found to be highest against Streotococcus mutans among the test organisms. Silver nanoparticle synthesised using natural substances can be used in control of oral pathogens.

Biogenic of silver nanoparticles using ayurveda tooth powder and its antibacterial activity of extended-spectrum beta-lactamases producing E.coli

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Abdullah A. Alarfajj ◽  
Mohammedsaleh Almallahi ◽  
Murugan A. Munusamy ◽  
Mickymaray Suresh ◽  
Wael Alturaiki
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Diffusion Method ◽  
Tem Analysis ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Extended Spectrum Beta Lactamases

Extended-spectrum beta-lactamases (ESBL) are enzymes produced by E. coli like some gram negative bacteria. The patients who are affected by ESBL producing bacteria facing a major problem and they may need different β- lactam antibiotics to treat the infection. But this extensive use of β- lactam antibiotics against ESPLs creating major public health threat.  As an alternative currently many eco- friendly, non-toxic, low cost nanoparticles are synthesizing by biogenic way used as an alternative for the β- lactam antibiotics. In the present study silver nanoparticles (AgNPs) were synthesized using Ayurveda Toothpowder. The synthesized AgNPs were characterized using ultraviolet (UV)-visible (vis) spectroscopy, scanning electron microscopy (SEM), and transmission electron microscope (TEM) analysis and finally the antibacterial activity was performed against ESBL producing bacteria by well diffusion method. Antibacterial tests against ESPL producing E.coli cells using biogenic synthesized AgNPs showed significant antibacterial effect at low concentration of AgNPs. The results proved that the biogenic synthesised nanoparticles using Toothpowder extract would help to arrest ESBL producing bacteria a

scholarly journals GREEN TECHNOLOGY MEDIATED SYNTHESIS OF SILVER NANOPARTICLES FROM MOMORDICA CHARANTIA FRUIT EXTRACT AND ITS BACTERICIDAL ACTIVITY

2017 ◽  
Vol 10 (3) ◽  
pp. 196 ◽  
Author(s):  
S C Joshi ◽  
Utkarsh Kaushik ◽  
Aproova Upadhyaya ◽  
Priyanka Sharma
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Nanoparticles ◽  
Atomic Force Microscope ◽  
Momordica Charantia ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Atomic Force ◽  
Vis Spectroscopy ◽  
Scanning Electron

ABSTRACTObjective: The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. Silver nanoparticlesare usually synthesized by chemicals and physical method, which are quite toxic and flammable in nature. This study deals with an environmentfriendly biosynthesis process of antibacterial silver nanoparticles using Momordica charantia fruit.Methods: AgNO3 (5 mM) was allowed to react with fruit extract of M. charantia. Biosynthesis of AgNPs was optimized by changing temperature,pH, and solvent. The silver nanoparticles so formed were characterized using ultraviolet-visible (UV-VIS) spectroscopy, Fourier transform infraredspectroscopy (FTIR), dynamic light scattering (DLS), atomic force microscope (AFM), and scanning electron microscopy (SEM).Results: UV-VIS spectra show absorption peak between 420 and 430 nm. The FTIR analysis showed the alcoholic, lactam, and nitro group presentin the plant extract, which were responsible for the reduction in AgNPs. The SEM images showed the size distribution of the nanoparticles and theaverage size was found to be 50-100 nm. By DLS analysis and AFM analysis, average sizes of the silver nanoparticles were of 150 nm. The results ofthese analyses confirmed the formation of silver nanoparticles. Silver nanoparticles were tested against Bacillus cereus and Staphylococcus epidermidisstrains using disc diffusion method and were found to be effective.Conclusion: Silver nanoparticles so synthesized in this study using fruit extract of M. charantia are simple, easy, and effective technique of nanoparticlesproduction.Keywords: Silver nanoparticles, Momordica charantia, Optimization, Antibacterial, Atomic force microscope, Scanning electron microscopy.

scholarly journals Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mona A. Alqahtani ◽  
Monerah R. Al Othman ◽  
Afrah E. Mohammed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Cancer Cell ◽  
Spherical Particles ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Hct 116

Abstract Recently, increase bacterial resistance to antimicrobial compounds issue constitutes a real threat to human health. One of the useful materials for bacterial control is Silver nanoparticles (AgNPs). Researchers tend to use biogenic agents to synthesize stable and safe AgNPs. The principal aim of this study was to investigate the ability of lichen in AgNPs formation and to find out their suppression ability to MDR bacteria as well as their cytotoxic activity. In the current study, lichens (Xanthoria parietina, Flavopunctelia flaventior) were collected from the south of the Kingdom of Saudi Arabia. Lichens methanolic extracts were used for conversion of Ag ions to AgNPs. Prepared biogenic AgNPs were characterized by Ultraviolet–Visible (UV–Vis) Spectroscopy, Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential and Energy-Dispersive X-ray Spectroscopy (EDS). Lichens Secondary metabolites were determined by Fourier-Transform Infrared Spectroscopy (FTIR) and Gas Chromatography–Mass Spectrometry (GC–MS). The antibacterial activity and synergistic effect of AgNPs were evaluated against pathogenic bacteria, including gram-positive; Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), and gram-negative; (Pseudomonas aeruginosa, Escherichia coli) as well as the reference strains (ATCC) using the agar disk diffusion method. Cytotoxic effect of biogenic AgNPs was tested against HCT 116 (Human Colorectal Cancer cell), MDA-MB-231 (Breast cancer cell), and FaDu (Pharynx cancer cell) by MTT test. TEM imaging showed well-dispersed spherical particles of 1–40 nm size as well as zeta size showed 69–145 nm. Furthermore, FTIR and GC–MS identified various lichen chemical molecules. On the other hand, the highest antibacterial activity of AgNPs was noticed against P. aeruginosa, followed by MRSA, VRE, and E. coli. AgNPs influence on gram-negative bacteria was greater than that on gram-positive bacteria and their synergistic effect with some antibiotics was noted against examined microbes. Moreover, higher cytotoxicity for biogenic AgNPs against FaDu and HCT 116 cell line in relation to MDA-MB-231 was noted. Given the current findings, the biogenic AgNPs mediated by lichens had positive antibacterial, synergistic and cytotoxic powers. Therefore, they might be considered as a promising candidate to combat the multi-drug resistance organisms and some cancer cells.

scholarly journals Antibacterial Activity of TiO2- and ZnO-Decorated with Silver Nanoparticles

2019 ◽  
Vol 3 (2) ◽  
pp. 61 ◽  
Author(s):  
Van Thang Nguyen ◽  
Viet Tien Vu ◽  
The Huu Nguyen ◽  
Tuan Anh Nguyen ◽  
Van Khanh Tran ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Visible Region ◽  
Light Irradiation ◽  
Hybrid Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Vis Spectroscopy ◽  
Xrd Patterns

This work emphasizes the use of the silver decorative method to enhance the antibacterial activity of TiO2 and ZnO nanoparticles. These silver-decorated nanoparticles (hybrid nanoparticles) were synthesized using sodium borohydride as a reducing agent, with the weight ratio of Ag precursors/oxide nanoparticles = 1:30. The morphology and optical properties of these hybrid nanoparticles were investigated using transmission electron microscopy (TEM), X-ray diffraction (XRD) patterns, and UV-Vis spectroscopy. The agar-well diffusion method was used to evaluate their antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria, with or without light irradiation. The TEM images indicated clearly that silver nanoparticles (AgNPs, 5–10 nm) were well deposited on the surface of nano-TiO2 particles (30–60 nm). In addition to this, bigger AgNPs (<20 nm) were dispersed on the surface of nano-ZnO particles (30–50 nm). XRD patterns confirmed the presence of AgNPs in both Ag-decorated TiO2 and Ag-decorated ZnO nanoparticles. UV-Vis spectra confirmed that the hybridization of Ag and oxide nanoparticles led to a shift in the absorption edge of oxide nanoparticles to the lower energy region (visible region). The antibacterial tests indicated that both oxide pure nanoparticles did not exhibit inhibitory effects against bacteria, with or without light irradiation. However, the presence of AgNPs in their hybrids, even at low content (<40 mg/mL), leads to a good antibacterial activity, and higher inhibition zones under light irradiation as compared to those in dark were observed.

Antimicrobial susceptibility of Pasteurella multocida isolated from swine and poultry

2009 ◽  
Vol 57 (3) ◽  
pp. 357-367 ◽  
Author(s):  
Boglárka Sellyei ◽  
Zsuzsanna Varga ◽  
Katalin Szentesi-Samu ◽  
Éva Kaszanyitzky ◽  
Tibor Magyar
Keyword(s):  
Pasteurella Multocida ◽  
First Generation ◽  
Antimicrobial Agents ◽  
Generation Cephalosporin ◽  
Fourth Generation ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Wide Range

Pasteurella multocida causes infectious diseases in a wide range of animal species. Antimicrobial therapy is still an effective tool for treatment. Generally, P. multocida isolates are susceptible to most of the widely used commercial antimicrobial agents but their excessive and unjustified use accelerates the emergence of resistant strains. We defined the antimicrobial sensitivity pattern of 56 P. multocida strains isolated from poultry (20) and swine [16 P. multocida toxin (PMT) positive and 20 PMT negative] to 16 widely applied antibiotics (apramycin, cefquinome, chloramphenicol, colistin, doxycycline, enrofloxacin, erythromycin, florfenicol, flumequine, neomycin, oxolinic acid, penicillin, trimethoprim potentiated sulphamethoxazole, sulphonamide compounds, tetracycline, tulathromycin) by the disk diffusion method. The majority of the strains was susceptible to most of the antimicrobial agents tested. However, the resistance to sulphonamides, tetracyclines, first-generation quinolones and aminoglycosides was remarkable, and thus the use of these compounds for the treatment of infection caused by P. multocida is not recommended. On the other hand, the antimicrobial activity of the classical penicillin, the newer macrolide (tulathromycin), the third-generation fluoroquinolone (enrofloxacin) and the fourth-generation cephalosporin (cefquinome) proved to be satisfactory against this bacterium.

scholarly journals Antimicrobial effect of natural kinds of toothpaste on oral pathogenic bacteria

2021 ◽  
Vol 15 (10) ◽  
pp. 1436-1442
Author(s):  
Serdar Demir ◽  
Gul Keskin ◽  
Nese Akal ◽  
Yasemin Zer
Keyword(s):  
Antimicrobial Activity ◽  
Adverse Effects ◽  
Pathogenic Bacteria ◽  
Natural Kinds ◽  
Aloe Vera ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Oral Pathogens ◽  
Inhibition Zones ◽  
Agar Well Diffusion Method

Introduction: Because of the adverse effects on human health of some antimicrobial ingredients in traditional toothpaste, consumers are increasingly turning to toothpastes with natural ingredients. This study evaluates the antimicrobial effect of toothpastes containing different natural active agents against three oral pathogens: Streptococcus mutans, Streptococcus sanguinis, and Enterococcus faecalis. Methodology: This study tested one traditional toothpaste and seven different natural toothpastes containing theobromine, aloe vera, miswak, propolis, chitosan, enzymes and probiotics. The agar-well diffusion method was used to test the antimicrobial effect. Inhibition zones formed around toothpastes after 24 hours of incubation were measured and the data collected were statistically analyzed. Results: Toothpastes containing theobromine and chitosan and the traditional toothpaste showed antimicrobial efficacy for all tested bacteria. Toothpastes containing aloe vera, miswak, and propolis were only effective on S. mutans, while toothpastes containing probiotics and enzymes did not show any antimicrobial effect on the bacteria. Among toothpastes with natural ingredients, the theobromine-containing toothpaste showed the highest efficacy on S. mutans, while the aloe vera- and propolis-containing toothpastes had the lowest efficacy (p < 0.05). Conclusions: Theobromine- and chitosan-containing toothpastes, which showed antimicrobial activity against all bacteria, can be recommended as alternatives to traditional toothpastes.

scholarly journals Hyaluronic Acid Mediated Zinc Nanoparticles against Oral Pathogens and Its Cytotoxic Potential

2020 ◽  
pp. 113-117
Author(s):  
V. Karthik ◽  
Lakshminarayanan Arivarasu ◽  
S. Rajeshkumar
Keyword(s):  
Hyaluronic Acid ◽  
Diffusion Method ◽  
Receptor Interaction ◽  
Bacterial Invasion ◽  
Technology Study ◽  
Cytotoxic Potential ◽  
Zinc Nanoparticles ◽  
Wide Range ◽  
Brine Shrimps ◽  
Oral Pathogens

Aim: To determine the Hyaluronic acid mediated zinc nanoparticles against oral pathogens and cytotoxic potential. Introduction: Hyaluronic acid is a non sulfated glycosaminoglycan. Bacterial invasion can also be repressed by an inhibitor Interfering with receptor interaction for bacterial invasion, Hyaluronic acid is an example of inhibitor.Nanoparticles is considered as one of the most promising studies in science and technology study  and  Maintaining the shape, size and distribution of nanoparticles helps in its function and interaction with other molecules. Materials and Methods: 0.1 g of Hyaluronic acid was added to a flask containing 100 ml of distilled water and heated for an hour. After observing the solubility, 0.574 g of Zn was added to the mixture and then kept on a magnetic stirrer for 1 hour at 100 degree Celsius. Anti microbial activity: Agar well diffusion and Disc diffusion method is used. Then incubated for 37 degree Celsius for 48 hour. The zone of inhibition is recorded. Cytotoxic potential: Different concentrations of Hyaluronic acid mediated zinc nanoparticles are incorporated to the wells. After 24 hrs the results were analysed. Results and Discussion: Hyaluronic mediated zinc nanoparticles is proved to be effective against a wide range of foodborne and clinically relevant Gram-positive and Gram-negative bacteria using several assays such as disk diffusion, agar or broth dilution.Hyaluronic acid mediated Zinc nanoparticles has high potent cytotoxic potential it had been proved with the help of brine shrimps. Conclusion: From the observed results, it has been concluded that Hyaluronic acid has a lot of medicinal values and it has antimicrobial activity and it has good cytotoxic potential.

scholarly journals Antimicrobial Potential of Rhizospheric Bacteria Streptobacillus sp.

2021 ◽  
Author(s):  
Jay Kishor Prasad ◽  
Riddha Dey ◽  
Richa Raghuwanshi
Keyword(s):  
Pathogenic Bacteria ◽  
Antagonistic Activity ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Biochemical Tests ◽  
Good Source ◽  
Rhizospheric Bacteria ◽  
Wide Range ◽  
And Staphylococcus Aureus ◽  
Agar Well Diffusion Method

Rhizospheric bacteria exhibiting antagonistic effects are a good source for the production of antibiotics. The antibiotics produced are naturally bactericidal or bacteriostatic in nature. In the present investigation, thirty-five rhizospheric bacteria were isolated from different soil samples. Agar well diffusion method, streak agar method, disc diffusion method and biochemical tests were performed to screen the ten antibiotic-producing bacteria. Among them, strain JRR34 selected on the basis of primary antagonistic activity was identified as Streptobacillus sp. Media optimisation was done to ensure maximum production of secondary metabolites. Streptobacillus sp. JRR34 showed good inhibitory activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The ethyl crude extract of Streptobacillus sp. JRR34 rhizobacteria possessing good antagonistic activity against a wide range of pathogenic bacteria can be a vital source of novel antibiotics.

scholarly journals Biosynthesis and characterization of silver nanoparticles using ginger spent and their antibacterial activity

2021 ◽  
pp. 174-179
Author(s):  
Sinthia Ganesan ◽  
Palanichamy Mehalingam ◽  
Govindan Sadasivam Selvam
Keyword(s):  
Silver Nanoparticles ◽  
Waste Product ◽  
Colour Change ◽  
Zingiber Officinale ◽  
Xrd Analysis ◽  
Sem Analysis ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Gram Positive Bacteria ◽  
Vis Spectroscopy

Ginger spent is the byproduct of spice industries that remove the essential oils of ginger (Zingiber officinale) for food industry and medicinal purposes. Ginger is a well known spice used often for seasoning in Indian cuisine. The de-oiled ginger has no specific use mostly goes to waste. Hence, we utilized this industrial waste product in the efficient synthesis of silver nanoparticles with the aid of UV irradiation from a solution of 1mM silver nitrate and spent extract in the ratio 9:1. Immediate colour change from pale yellow to dark brown was noted indicating the rapid synthesis of silver nanoparticles. These nanoparticles were centrifuged, dried and well characterized. UV Vis Spectroscopy, XRD analysis, Zeta potential and SEM analysis was carried out. It was commendable that the size of the nanoparticles fell well within the upper limit of 100nm. Agar well diffusion method was used to screen the antimicrobial activity of the well characterized silver nanoparticles. They were tested against seven pathogenic strains of three gram negative bacteria (Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa) three gram positive bacteria(Bacillus subtilis, Staphylococcus aureus and Streptococcus faecalis) and a fungus (Candida  albicans). It was seen that the zone of inhibition(ZOI) in well plate method  increased on increasing the concentration of silver nanoparticles. Further studies could lead to the application of these silver nanoparticles in medicine.

scholarly journals Microwave Assisted Green Synthesis of Silver Nanoparticles using Pomegranate Peel Extract: Characterization and Antibacterial Activity Studies

2019 ◽  
Vol 32 (2) ◽  
pp. 458-462
Author(s):  
Gugulothu Yaku ◽  
Bandi Rajkumar ◽  
T.V.D. Prasad Rao
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Diffusion Method ◽  
Microwave Assisted Synthesis ◽  
Pomegranate Peel ◽  
Stabilizing Agent ◽  
X Ray ◽  
Microwave Assisted ◽  
Pomegranate Peel Extract

In the present work, a simple and low cost and eco-friendly technique is applied for the microwave assisted synthesis of silver nanoparticles (AgNPs) using the extract of pomegranate peel wastes, which does not require any use of external stabilizing agent. The extract of pomegranates peelings waste served as a reducing as well as capping/stabilizing agent. The synthesized silver nanoparticles were characterized by using powder X-ray diffraction (XRD), transmission eelectron microscopy (TEM), FT-IR, UV-visible spectroscopy and scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX). The stability of AgNPs was analyzed by zeta potential measurements. The antibacterial activity of synthesized AgNPs was also evaluated on six Gram-positive and Gram-negative bacteria using agar well diffusion method.

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