scholarly journals Protozoan Parasites of Rodents and Their Zoonotic Significance in Boyer-Ahmad District, Southwestern Iran

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Zeinab Seifollahi ◽  
Bahador Sarkari ◽  
Mohammad Hossein Motazedian ◽  
Qasem Asgari ◽  
Mohammad Javad Ranjbar ◽  
...  
Keyword(s):  
Intestinal Parasites ◽  
Molecular Study ◽  
Wild Rodents ◽  
Intestinal Protozoa ◽  
Tissue Samples ◽  
Modified Agglutination Test ◽  
Protozoan Infection ◽  
Stool Samples ◽  
Southwestern Iran ◽  
Giardia Muris

Backgrounds. Wild rodents are reservoirs of various zoonotic diseases, such as toxoplasmosis, babesiosis, and leishmaniasis. The current study aimed to assess the protozoan infection of rodents in Boyer-Ahmad district, southwestern Iran.Materials and Methods. A total of 52 rodents were collected from different parts of Boyer-Ahmad district, in Kohgiluyeh and Boyer-Ahmad province, using Sherman live traps. Each rodent was anesthetized with ether, according to the ethics of working with animals, and was dissected. Samples were taken from various tissues and stool samples were collected from the contents of the colon and small intestines. Moreover, 2 to 5 mL of blood was taken from each of the rodents and the sera were examined for anti-Leishmaniaantibodies, by ELISA, or anti-T. gondiiantibodies, by modified agglutination test (MAT). DNA was extracted from brain tissue samples of each rodent and PCR was used to identify the DNA ofT. gondii.Results. Of the 52 stool samples of rodents studied by parasitological methods, intestinal protozoa infection was seen in 28 cases (53.8%). From 52 rodents, 19 (36.5%) were infected withTrichomonas, 10 (19.2%) withGiardia muris, and 11 (21.2%) withEntamoebaspp. Also, 10 cases (19.2%) were infected withBlastocystis, 3 (5.8%) were infected withChilomastix, 7 (13.5%) were infected withEndolimax, 1 (1.9%) was infected withRetortamonas, 3 (5.77%) were infected withT. gondii, and 6 (11.54%) were infected withTrypanosoma lewisi. Antibodies toT. gondiiwere detected in the sera of 5 (9.61%) cases. Results of the molecular study showedT. gondiiinfection in 3 (5.77%) of the rodents. Findings of this study showed that rodents in Kohgiluyeh and Boyer-Ahmad province, southwestern Iran, are infected with several blood and intestinal parasites; some of them might be potential risks to residents and domestic animals in the region.

scholarly journals Prevalence of Giardia intestinalis Infection in Schistosomiasis-Endemic Areas in South-Central Mali

2019 ◽  
Vol 4 (2) ◽  
pp. 86 ◽  
Author(s):  
Hassan K.M. Fofana ◽  
Maren Schwarzkopf ◽  
Mama N. Doumbia ◽  
Rénion Saye ◽  
Anna Nimmesgern ◽  
...  
Keyword(s):  
Intestinal Parasites ◽  
Epidemiological Data ◽  
Giardia Intestinalis ◽  
Diagnostic Tools ◽  
Intestinal Protozoa ◽  
Control Programs ◽  
South Central ◽  
Concurrent Infections ◽  
Intestinal Pathogens ◽  
Stool Samples

Intestinal parasite infections are frequent causes of diarrhea and malnutrition among children in the tropics. Transmission of helminths and intestinal protozoa is intimately connected with conditions of poverty, including inadequate sanitation and hygiene. Concurrent infections with several intestinal pathogens may lead to excess morbidity. Yet, there is a paucity of epidemiological data from Mali. In this study, stool samples from 56 individuals, aged 2–63 years, from Bamako and Niono, south-central Mali were examined for intestinal parasites using stool microscopy. Additionally, stool samples were subjected to a rapid diagnostic test (RDT) and polymerase chain reaction (PCR) for the detection of Cryptosporidium spp. and Giardia intestinalis. The predominant pathogens were Schistosoma mansoni and G. intestinalis with prevalences of 41% and 38%, respectively. Hymenolepis nana was detected in 4% of the participants, while no eggs of soil-transmitted helminths were found. Concurrent infections with G. intestinalis and S. mansoni were diagnosed in 16% of the participants. For the detection of G. intestinalis, PCR was more sensitive (100%) than RDT (62%) and microscopy (48%). As helminth-protozoa coinfections might have important implications for morbidity control programs, future studies should employ diagnostic tools beyond stool microscopy to accurately assess the co-endemicity of giardiasis and schistosomiasis.

scholarly journals Prevalence of intestinal parasitic infections among patients attending in a tertiary care hospital of eastern region of Nepal – A retrospective, laboratory based study

2017 ◽  
Vol 8 (3) ◽  
pp. 55-59 ◽  
Author(s):  
Ratna Baral ◽  
Pramod Jha ◽  
Ritu Amatya ◽  
Basudha Khanal
Keyword(s):  
Intestinal Parasites ◽  
Tertiary Care ◽  
Parasitic Infection ◽  
Parasitic Infections ◽  
Eastern Region ◽  
Care Hospital ◽  
Medical Sciences ◽  
Intestinal Protozoa ◽  
Intestinal Parasitosis ◽  
Stool Samples

Background: Intestinal parasitosis still constitutes one of the major causes of public healthproblems in the world, particularly in developing countries. Nepal is a small, impoverished country prevalent to infectious diseases, including intestinal parasitosis. Poverty, lack of awareness, failure to practice proper hand washing after defecation, practice of open defecation, unsafe drinking water and use of improper toilets are some of the reasons causing of parasitic infections.Aims and Objective: To determine the types of intestinal parasites in stool samples of patients attending to hospital. Materials and Methods: Stools specimens collected in standard stool vial were submitted in microbiology (2006-2010) unit at BP Koirala Institute of Health Sciences hospital (BPKIHS) were examined for intestinal parasites by direct wet mount using normal saline (0.9%) and lugol’s iodine (0.5%).Results: A total of 11,791 stool samples (2928 in 2006, 2238 in 2007, 2151 in 2008, 2344 in 2009 and 2130 in 2010) were submitted to the Parasitology section of Department of Microbiology BPKIHS were included in the analysis. Of these, 675 (5.72%) were positive intestinal protozoa and 289 (2.45%) for intestinal helminths. Giardia intestinalis accounted for the most prevalent parasitic infection (3.34%) followed by Entamoeba histolytica/E. dispar (1.96%) and Hookworm (0.97%). A parasitic infection was observed to be highest among 20-50 years of age group and lowest in the less than 5 years group of subjects.Conclusion: Intestinal parasitosis is a common problem. Amoebiasis, giardiasis and the common intestinal helminthes Ascaris lumbricoides, hookworm, and Trichuris trichiura were prevalent in our settings.Asian Journal of Medical Sciences Vol.8(3) 2017 55-59

scholarly journals Epidemiological and Molecular Characterization of Blastocystis Infection in Children Attending Daycare Centers in Medellín, Colombia

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 669
Author(s):  
Maria I. Osorio-Pulgarin ◽  
Adriana Higuera ◽  
Juan C. Beltran-Álzate ◽  
Miryan Sánchez-Jiménez ◽  
Juan David Ramírez
Keyword(s):  
Molecular Characterization ◽  
Intestinal Parasites ◽  
Phylogenetic Reconstruction ◽  
Kappa Index ◽  
Blastocystis Infection ◽  
Conventional Pcr ◽  
Intestinal Protozoa ◽  
Daycare Centers ◽  
Stool Samples

Background: The present study aims to perform an epidemiological and molecular characterization of Blastocystis infection in a child population attending daycare centers of Medellín, Colombia. Methods: A total of 265 children aged 0–5 years were enrolled in five children’s centers in urban sectors of Medellín, northwestern Colombia. Stool samples were taken to identify intestinal parasites by direct examination, Ritchie–Frick concentration, and molecular identification of Blastocystis by conventional PCR and subtype (ST) identification by PCR barcoding with subsequent phylogenetic reconstruction. Kappa index was calculated to evaluate the agreement between microscopy and PCR for the diagnosis of Blastocystis. Results: The prevalence of intestinal protozoa was 36.6% (97/265), with Blastocystis as the most frequent parasitic protozoan at 15.8% (42/265), followed by Giardia intestinalis at 15.5% (41/265) and Endolimax nana at 15.1% (40/265). The prevalence of Blastocystis by PCR was 53.2% (141/265), the subtypes identified were ST3 at 30.5% (18/59), ST2 at 23.7% (14/59), ST1 at 20.3% (12/59), and with less frequency, ST4 at 5.1% (3/59), ST6 at 1.7% (1/59) and ST16 at 15.3% (9/59) allele 162. Conclusion: This study provides the first genetic characterization of Blastocystis subtypes circulating in a population of Medellín, Colombia, and also updates the epidemiology of Blastocystis subtypes in the world with the first identification of ST16 in humans.

scholarly journals Intestinal parasites of pets and other house-kept animals in Moscow

2019 ◽  
Vol 56 (2) ◽  
pp. 108-117 ◽  
Author(s):  
O. P. Kurnosova ◽  
M. V. Arisov ◽  
I. M. Odoyevskaya
Keyword(s):  
Guinea Pigs ◽  
Parasite Species ◽  
Intestinal Parasites ◽  
Parasitic Infections ◽  
Intestinal Protozoa ◽  
Exotic Animals ◽  
Source Of Infection ◽  
Protozoan Infection ◽  
The Family ◽  
Moscow City

SummaryA study screening pet animals (dogs, cats, chinchillas, ferrets, guinea pigs, rabbits, primates, reptiles, and hedgehogs) within Moscow city limits for intestinal parasitic diseases has been conducted over a period of 6 years. According to the study, parasitic infections caused by intestinal protozoa are found in pet animals more frequently than by intestinal helminths. Although dogs and cats exhibit the highest level of diversity of intestinal parasite species, in the group of exotic animals, helminth infection are found much less frequently and parasitic fauna is represented mostly by intestinal protozoa with a high percentage of mixed infection. The most widespread helminth infeсtion of dogs and cats is toxocarosis (respectively 2.5 and 5.7 %) and the most widespread protozoan infection is Giardia sp. (9.8 and 4.6 %). Giardia sp. was found in 47.4 % of chinchillas, Cryptosporidium sp. was more frequently found in ferrets (6.55 %), protozoa from the family Trichomonadida was found in guinea pigs (9 %), Eimeria sp. in rabbits (13.9 %), Acanthocephala in primates (15.7 %), and eggs from the generaOxyurida (59 %), along with protozoa from the family Trichomonadida, in reptiles. Capillaria sp. was most prevalent in hedgehogs (33.4 %). Acanthocephala eggs, as well as protozoa from the Giardia and Entamoeba genera, were more frequently found in primates. Parasites common to animals and humans, which may become a source of infection for the latter under certain conditions, have been identified in pet animals.

Intestinal Parasites and Salmonella typhi Infection among Food-handlers in Port Harcourt Metropolis, Nigeria

2020 ◽  
pp. 1-9
Author(s):  
Adedokun, Ambali Amudatu ◽  
Onosakponome Evelyn Orevaoghene ◽  
Austin E. Abah
Keyword(s):  
Intestinal Parasites ◽  
Salmonella Typhi ◽  
Food Handlers ◽  
Intestinal Protozoa ◽  
Concentration Technique ◽  
Salmonella Infections ◽  
Port Harcourt ◽  
Stool Samples ◽  
Handling Practices ◽  
Food Handling Practices

Background: Intestinal parasites and Salmonella infections are of main public health concerns in Nigeria and the world, especially in developing countries where access to safe water and hygienic food handling practices by food handlers is a challenge. Consequently, food-handlers play a major role in the transmission of these types of foodborne diseases. Objective: This study was to determine the preponderance of intestinal parasites and Salmonella typhi among food-handlers in selected areas in Port Harcourt. Materials and Methods: A total of 480 food-handlers (195 males and 285 females) of various educational levels were sampled. Stool samples were collected and analyses were carried out using direct smear examination and formol-ether concentration technique - adopted for investigation of intestinal parasites and deoxycholate citrate agar and Salmonella-Shigella agar for the cultivation of Salmonella typhi. Results: Of 480 food-handlers examined, comprises of 195(40.6%) males and 285(59.4%) females, - majority (85.4%) were young adults aged 20-40 years. 47.9% of them - had education above secondary educational level. The preponderance of intestinal parasites was 48(10.0%) and implicated were Ascaris lumbricoides (41.7%), hookworm (33.3%) and Trichuris trichiura (25.0%). No intestinal protozoa were identified. Prevalence of 30(6.3%) was recorded for Salmonella typhi. Conclusion: Prevalence of Intestinal Parasites and S.typhi infection is relatively low among food handlers in Port Harcourt, but indicates the important role food handlers play in the transmission of the infection. Therefore, awareness should be step-up for the food handlers to avoid escalation of these infections.

scholarly journals Parasitic infections in Swiss children: Are we overtesting?

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Corinne Légeret ◽  
Céline Rüttimann ◽  
Hans Fankhauser ◽  
Henrik Köhler
Keyword(s):  
Retrospective Study ◽  
Positive Result ◽  
Pediatric Patients ◽  
Intestinal Parasites ◽  
Gastrointestinal Symptoms ◽  
International Travel ◽  
Parasitic Infections ◽  
Stool Samples ◽  
Stool Specimens

Abstract Background A wide variation of causes can lead to gastrointestinal symptoms in children- an infection with parasites is one of them. The expansion of international travel might lead to an increase in testing children for a correspondent infection. Currently there are no guidelines available, which patients should be tested for a possible parasitical infection. The aim of the study was to characterize Swiss children suffering from intestinal parasites, in order to provide more knowledge for the clinician who should be tested. Methods This is a retrospective study of Swiss pediatric patients, whose stools have been tested for parasites and helminths. Results A total of 1855 stool samples, belonging to 572 different children with an average age of 7.9 years, were tested within a 10-year period. The prevalence of a positive result was 4.2%, of which all were positive for Blastocystis, and 12.5% had a co-infection with Endolimax nana. Conclusion Immigrants, immune compromised children with diarrhea and pediatric patients with bloody or protracted diarrhea should have 2 different stool specimens examined for a possible parasitical infection.

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

scholarly journals Prevalence and Correlates of Intestinal Parasites among Patients Admitted to Mirembe National Mental Health Hospital, Dodoma, Tanzania

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Azan A. Nyundo ◽  
David Z. Munisi ◽  
Ainory P. Gesase
Keyword(s):  
Neglected Tropical Diseases ◽  
Intestinal Parasites ◽  
Psychiatric Patients ◽  
Parasitic Infection ◽  
Parasitic Infections ◽  
Intestinal Parasitic Infection ◽  
Psychiatric Facility ◽  
Concentration Data ◽  
Cross Sectional ◽  
Stool Samples

Background. Neglected tropical diseases continue to be one of the leading causes of morbidity and mortality in the developing world. Psychiatric patients are among groups at risk for parasitic infection although control and monitoring programs largely overlook this population. This study aimed at determining prevalence and factors associated with intestinal parasitic infection among patients admitted to a psychiatric facility.Method. The study followed cross-sectional design; all the residing patients that met the inclusion criteria were included in the survey. Stool samples were collected and examined by direct wet preparation and formol-ether concentration. Data were analyzed with STATA version 12.1; Chi-square test was computed to determine the level of significance atpvalue < 0.05.Results.Of all 233 patients who returned the stool samples, 29 (12.45%) screened were positive for an intestinal parasite. There was no significant association between parasite carriage and age, sex, or duration of hospital stay.Conclusion. The study shows that intestinal parasitic infection is common among patients in a psychiatric facility and highlights that parasitic infections that enter through skin penetration may be a more common mode of transmission than the oral route. Furthermore, the study underscores the need for surveillance and intervention programs to control and manage these infections.

scholarly journals Survey of iodine deficiency and intestinal parasitic infections in school-going children: Bie Province, Angola

2010 ◽  
Vol 13 (9) ◽  
pp. 1314-1318 ◽  
Author(s):  
Mark Tomlinson ◽  
Vera Adams ◽  
Mickey Chopra ◽  
Pieter Jooste ◽  
Emmerentia Strydom ◽  
...  
Keyword(s):  
Iodine Deficiency ◽  
International Health ◽  
Target Population ◽  
Cross Sectional Study ◽  
Parasitic Infections ◽  
Intestinal Protozoa ◽  
Cross Sectional ◽  
Helminth Infections ◽  
Stool Samples ◽  
The Government

AbstractObjectiveTo obtain baseline pre-intervention prevalence levels of iodine deficiency and parasitic and helminth infections in school-going children in Bie Province, Angola.DesignA cross-sectional study conducted in randomly selected schools. The target population was children aged 6–10 years.SettingBie Province, Angola.SubjectsA total of 1029 children sampled, with 791 stool samples and 826 urine samples collected from twenty-four schools.ResultsWidespread severe and moderate deficiencies in iodine. Children in five schools were severely iodine deficient. All sampled schoolchildren were iodine deficient to a greater or lesser extent. In all, 80 % of all children across the twenty-four schools were infected with one or a combination of intestinal helminths and intestinal protozoa.ConclusionsThese findings have serious implications for the cognitive development of Angolan children, as well as for Angola’s development in terms of productivity and economic potential. It is strongly recommended that the provincial and national Ministries of Health, in collaboration with international health agencies, immediately plan and implement a strategy to provide sufficient iodine through iodised oil capsules and iodised salt to the iodine-deficient population. National coalitions need to be strengthened among the government, partners and salt producers. It is also recommended that all the children in schools be de-wormed for multiple helminth species at least twice a year.

Protozoan infection in archaeological material

1970 ◽  
Vol 80 (1) ◽  
Author(s):  
Marcelo Luiz Carvalho Gonçalves ◽  
Cassius Schnell ◽  
Luciana Sianto ◽  
Francoise Bouchet ◽  
Mathieu Le Bailly ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
19Th Century ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Human Feces ◽  
Archaeological Material ◽  
The Past ◽  
Protozoan Infection ◽  
Protein Molecules ◽  
The 19Th Century

The identification of parasites in ancient human feces is compromised by differential preservation of identifiable parasite structures. However, protein molecules can survive the damage of the environment. It was possible to detected antigen of Entamoeba histolytica and Giardia duodenalis in historic and prehistoric human fecal remains using two enzyme immunoassay (ELISA) kits with monoclonal antibodies specific for E. histolytica and G. duodenalis, respectively. Specimens of desiccated feces and ancient latrine sediment from the New and the Old World were examined. The ELISA detected E. histolytica antigen in samples from Argentina, USA, France, Belgium, and Switzerland, dated to about 5300 years BP to the 19th Century AD. G. duodenalis antigen was detected in samples from USA, Belgium, and Germany, dated to about 1200 AD, 1600 AD, and 1700 AD. The detection of protozoan antigen using immunoassays is a reliable tool for the study of intestinal parasites in the past.

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