scholarly journals Differential Expression of IL-36 Family Members and IL-38 by Immune and Nonimmune Cells in Patients with Active Inflammatory Bowel Disease

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Gabriela Fonseca-Camarillo ◽  
Janette Furuzawa-Carballeda ◽  
Emilio Iturriaga-Goyon ◽  
Jesús K. Yamamoto-Furusho
Keyword(s):  
Gene Expression ◽  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Members ◽  
Control Group ◽  
Clinical Activity ◽  
Gut Inflammation ◽  
Active Inflammatory Bowel Disease ◽  
Inflammatory Bowel

IL-1 family includes IL-38 (IL-1F10) and the subfamily of IL-36 and is the central mediators of inflammatory diseases, including pustular psoriasis, atopic dermatitis, rheumatoid arthritis, and gut inflammation. The purpose of the study was to evaluate on tissue of the patients with inflammatory bowel disease (IBD), the IL-36α, IL-36β, IL-36γ, IL-36Ra, and IL-38 gene and cell expression and its correlation with clinical activity. Patients and Methods. A cross-sectional and comparative study was performed. Seventy patients with IBD and 30 noninflamed non-IBD controls were enrolled. Gene expression was measured by RT-PCR. Protein expression was detected by double-staining immunohistochemistry. Results. The mRNA expression of IL-36 family members but not IL-38 was increased in colonic mucosa from patients with active ulcerative colitis versus Crohn’s disease group and noninflammatory control group (P<0.05). However, only gene expression of IL-38 was increased in tissue from patients with inactive ulcerative colitis versus active disease and control group (P<0.005). Conversely, gene expression of IL-36Ra was significantly higher in colonic tissue from patients with active versus inactive ulcerative colitis and noninflamed control group (P<0.05). A differential protein overexpression of IL-36α, IL-36β, IL-36γ, IL-36Ra, and IL-38 by intestinal epithelial cells, macrophages, CD8+ T cells, and/or versus dendritic cells (pDCs) was found in patients with active inflammatory bowel disease compared with noninflamed controls. Conclusion. IL-38 and IL-36 family members’ expression was increased by immune and nonimmune cells in patients with active inflammatory bowel disease. These cytokines and IL-36Ra might represent novel therapeutic targets in patients with gut inflammation.

scholarly journals The Autoimmune Protocol Diet Modifies Intestinal RNA Expression in Inflammatory Bowel Disease

2019 ◽  
Vol 1 (3) ◽  
Author(s):  
Anita Chandrasekaran ◽  
Bhuvan Molparia ◽  
Ehsaan Akhtar ◽  
Xiaoyun Wang ◽  
James D Lewis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Rna Expression ◽  
Inflammatory Pathways ◽  
Scripps Clinic ◽  
Inflammatory Bowel ◽  
La Jolla

Lay Summary Researchers from the Scripps Clinic in La Jolla, CA recently looked at gene expression to better understand the role that diet plays in inflammatory bowel disease. Their findings suggest that diet may help modify inflammatory pathways in people with ulcerative colitis.

scholarly journals Dynamics of leukocytic blood parameters depending on the activity of inflammatory bowel disease in children

2021 ◽  
Vol 24 (3) ◽  
pp. 148-156
Author(s):  
Elena L. Semikina ◽  
Valeriya S. Tsvetkova ◽  
Alexander S. Potapov ◽  
Elena A. Kopyltsova ◽  
Andrey P. Fisenko ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Bowel Disease ◽  
Absolute Number ◽  
Blood Parameters ◽  
Clinical Activity ◽  
Inflammatory Bowel ◽  
The Absolute

Aim: to establish the patterns of changes in leukocyte blood parameters to determine their diagnostic significance in the different activity of Crohn’s disease (CD) and ulcerative colitis (UC) in children. Materials and methods. 370 5-18-year children (198 boys, 172 girls) with inflammatory bowel disease (IBD) were examined. Leukocytic blood parameters, concentrations of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), faecal calprotectin (FC) levels were determined. Changes in clinical activity in Crohn’s disease (CD) and ulcerative colitis (UC) cases were determined using pediatric activity indices: PCDAI and PUCAI, respectively. Analysis of endoscopic activity in 160 CD and UC patients was determined using the scales: SES-CD and UCEIS, respectively. Results. It was found that with an increase in the clinical activity of CD in children, the absolute numbers of leukocytes, lymphocytes and monocytes did not change significantly. In contrast, a significant increase in neutrophil reactivity was noted. In patients with high endoscopic CD activity an increase in the absolute number of monocytes and neutrophil reactivity was found. In patients with high clinical activity of UC, an increase in the number of leukocytes and the absolute number of neutrophils was found. At the same time, the granularity and reactivity of neutrophils increased from remission to moderate and high clinical activity of UC. In children with high endoscopic UC activity, the number of leukocytes, the absolute number of neutrophils and monocytes increased. At the same time, the granularity of neutrophils significantly increased as the endoscopic activity of UC increased from remission to moderate activity. Leukocyte parameters in UC patients correlated moderately with FC level, and the absolute number of neutrophils was closely related to its content. The granularity and reactivity of neutrophils positively correlated with ESR and CRP levels in children with CD and CRP concentrations in children with UC. Conclusion. The established patterns of changes in the quantitative composition of leukocytes and their functional properties (reactivity and granularity) are directly determined by the different activity of IBD in children and have diagnostic value

Specific quality of life factors in patients with inflammatory bowel disease

2012 ◽  
Vol 153 (38) ◽  
pp. 1511-1519 ◽  
Author(s):  
Beáta Nagy ◽  
Réka Laczkóné Majer
Keyword(s):  
Quality Of Life ◽  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Content Analysis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Bowel Disease ◽  
Control Group ◽  
Inflammatory Bowel

Introduction: Inflammatory bowel disease is a chronic disease with a fluctuating course and unknown origin. Its two major forms are Crohn’s disease and ulcerative colitis. Objective: The authors attempted to analyse the quality of life of patients with inflammatory bowel disease as compared to healthy persons and patients with asthma in order to identify specific factors which most significantly affect the quality of life of these patients. Method: 269 subjects participated in the study (control group, 115 subjects; Crohn’s disease, 67 patients; ulcerative colitis, 25 patients; asthma, 62 patients). The following methods were used: WHO-5, IIRS, SIBDQ and AQLQ(S) questionnaires and content analysis. Results: Patients with inflammatory bowel disease had the worst quality of life with respect to both subjective well-being and burden of disease. The results obtained from questionnaires developed for the analysis of quality of life indicated that extraintestinal symptoms and emotional problems are the major factors which interfere with the quality of life of patients with inflammatory bowel disease. These findings were confirmed by content analysis of interviews with patients. Conclusions: The authors recommend that more attention should be paid to improving the quality of life of patients with inflammatory bowel disease and helping them to live with the illness, especially at the level of interpersonal relations. Orv. Hetil., 2012, 153, 1511–1519.

scholarly journals Pilot study: duodenal MDR1 and COX2 gene expression in cats with inflammatory bowel disease and low-grade alimentary lymphoma

2017 ◽  
Vol 20 (8) ◽  
pp. 759-766 ◽  
Author(s):  
Jorge Castro-López ◽  
Mariana Teles ◽  
Camino Fierro ◽  
Karin Allenspach ◽  
Marta Planellas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Activity Index ◽  
Clinical Signs ◽  
Control Group ◽  
Low Grade ◽  
Mrna Levels ◽  
Chronic Enteropathy ◽  
Inflammatory Bowel

Objectives Multidrug resistance 1 (MDR1) encodes a protein called P-glycoprotein (P-gp), which serves as an efflux pump membrane protein implicated in intestinal homeostasis and drug resistance. Cyclooxygenase-2 (COX2) is a key enzyme in the synthesis of proinflammatory prostaglandins, tumourigenesis and in mucosal defence. Despite the importance of MDR1 and COX2, changes in their mRNA levels have not been studied in cats with inflammatory bowel disease (IBD) and low-grade alimentary lymphoma (LGAL). The present study aimed to determine the mRNA levels of MDR1 and COX2 in cats with IBD and LGAL, and to evaluate their correlation with clinical signs, histological severity and between genes. Methods Cats diagnosed with IBD (n = 20) and LGAL (n = 9) between 2008 and 2015 were included in the current study. Three healthy animals composed the healthy control cats group in which endoscopy was performed immediately before the ovariohysterectomy. All duodenal biopsy samples were obtained by endoscopy. Feline chronic enteropathy activity index was calculated for all cases. IBD histopathology was classified according to severity. MDR1 and COX2 mRNA levels were determined by absolute reverse transcriptase-quantitative real-time PCR. Results Statistically significant differences were observed for MDR1 and COX2 mRNA levels between the IBD and LGAL groups. No correlations were observed between molecular gene expression, feline chronic enteropathy activity index and histological grading for IBD, and between MDR1 and COX2 genes. However, a positive statistically significant correlation was observed between MDR1 and COX2 expression in the duodenum of cats. Conclusions and relevance MDR1 and COX2 gene expression is increased in cats with LGAL compared with cats with IBD. The control group tended to have lower values than both diseased groups. These results suggest that these genes may be involved in the pathogenesis of IBD or LGAL in cats.

scholarly journals Colonoscopy in patients with inflammatory bowel disease: self-reported experience, understanding, anxieties and tolerance of the procedure

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 927
Author(s):  
Samantha Morgan ◽  
Christopher Alexakis ◽  
Lucy Medcalf ◽  
Vivek Chhaya ◽  
Penny Neild ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Bowel Preparation ◽  
Bowel Disease ◽  
Clinical Activity ◽  
Gender And Age ◽  
Significant Difference ◽  
Inflammatory Bowel ◽  
Specific Factors ◽  
Structured Questionnaire

Objective: To address Inflammatory Bowel Disease (IBD) patients’ attitudes, understanding and tolerance of colonoscopy and assess whether there are specific factors that influence these parameters.Design: structured questionnaire-based survey. Tolerance of various aspects of colonoscopy procedure graded on a scale 1-5, 5 representing most intolerance/burden (worries/concerns about the procedure/risks, bowel preparation, disruption to life, procedural discomfort and travel concerns).Setting: London teaching hospital - St Georges HospitalPatients: Consecutive patients with established IBD attending the specialist IBD clinicResults: 98 patients responded (46% male). Mean age was 43.2 years. 33 had Ulcerative Colitis (UC), 50 had Crohn’s Disease (CD), and 11 were unsure of diagnosis. Mean number of colonoscopies was 3.7. Females were more worried about the procedure than males (3.0 vs 2.1, p<0.05), were less tolerant of bowel preparation (3.5 vs 2.3, p<0.05), experienced more disruption to their lives (2.9 vs 1.9, p<0.05) and were more troubled by travel concerns (2.0 vs 1.4, p<0.05). Patients with the disease for ≥ 5 years experienced significantly more discomfort than patients with the disease for a shorter duration (3.2 vs 2.7 p<0.05). Patients aged ≥55 years are significantly less worried about the procedure (2.7 vs 2.0, p = <0.05) and tolerate the bowel preparation better (3.1 vs 2.4, p = <0.05). The majority of the patients felt colonoscopy was ‘bearable’ (53%) with only 13% describing it as ‘very unpleasant’. 55% would have the procedure ‘as frequently as required’ if their physician felt it appropriate.Conclusions: Our research highlights a significant difference in the perception of colonoscopy by gender and age. Overall our findings reveal a preparedness to undergo colonoscopy as required despite an increasing requirement for this test. The differences highlighted should prompt endoscopy units to accommodate and make allowances for these different perceived tolerance in routine clinical activity.

scholarly journals ORAL MUCOSA LESIONS AND ORAL SYMPTOMS IN INFLAMMATORY BOWEL DISEASE PATIENTS

2015 ◽  
Vol 52 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Nuno LARANJEIRA ◽  
Jorge FONSECA ◽  
Tânia MEIRA ◽  
João FREITAS ◽  
Sara VALIDO ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Oral Mucosa ◽  
Bowel Disease ◽  
Statistical Significance ◽  
Control Group ◽  
Study Group ◽  
Immunosuppressant Therapy ◽  
Oral Symptoms ◽  
Inflammatory Bowel

Background Inflammatory Bowel Disease is known for its extra intestinal manifestations, the oral cavity is no exception. Objectives The aim of this study was to evaluate the association between Inflammatory Bowel Disease and oral mucosa lesions and symptoms, and complementary to evaluate their possible relation with oral hygiene, smoking habits, drug therapy, duration and activity of the disease. Methods Patients were selected from the Gastroenterology Clinic of a Portuguese tertiary referral hospital. This sample consisted of 113 patients previously diagnosed with ulcerative colitis or Crohn’s disease along with a control group of 58 healthy individuals that were accompanying the study group patients to their appointments. Clinical interviews and clinical examinations were performed for data collection. Results The patients in the study group were more affected by oral symptoms (P=0.011), and showed a trend towards a higher incidence of oral mucosal lesions, even though statistical significance was not reached (8.8% versus 3.4% in the control group; P=0.159). Patients in active phase were the most affected. No differences were detected between Crohn’s disease and ulcerative colitis, or concerning smoking habits. The corticosteroid and immunosuppressant therapy seemed to increase the incidence of oral symptoms (P=0.052). The oral mucosa lesions increased and the oral symptoms decreased over the course of the disease, however without statistical significance. Conclusion Oral mucosa’s lesions and oral symptoms were positively associated with Inflammatory Bowel Disease, mainly during disease activity periods and conceivably, associated with corticosteroid and immunosuppressant therapy.

scholarly journals MicroRNAs in Colon Tissue of Pediatric Ulcerative Pancolitis Patients Allow Detection and Prognostic Stratification

2021 ◽  
Vol 10 (6) ◽  
pp. 1325
Author(s):  
Petr Jabandziev ◽  
Tatsuhiko Kakisaka ◽  
Julia Bohosova ◽  
Tereza Pinkasova ◽  
Lumir Kunovsky ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Pediatric Patients ◽  
Characteristic Curve ◽  
Control Group ◽  
Colon Tissue ◽  
Candidate Mirnas ◽  
Prognostic Stratification ◽  
Inflammatory Bowel

Prevalence of inflammatory bowel disease has been on the rise in recent years, especially in pediatric populations. This study aimed to provide precise identification and stratification of pediatric patients with diagnosed ulcerative colitis (UC) according to the severity of their condition and the prediction for standard treatment according to the specific expression of candidate miRNAs. We enrolled consecutive, therapeutically naïve, pediatric UC patients with confirmed pancolitis. We examined formalin-fixed paraffin-embedded specimens of colonic tissue for the expression of 10 selected candidate miRNAs. We performed receiver operating characteristic curve analysis, using area under the curve and a logistic regression model to evaluate the diagnostic and predictive power of the miRNA panels. Sixty patients were included in the final analysis. As a control group, 18 children without macroscopic and microscopic signs of inflammatory bowel disease were examined. The combination of three candidate miRNAs (let-7i-5p, miR-223-3p and miR-4284) enabled accurate detection of pediatric UC patients and controls. A panel of four candidate miRNAs (miR-375-3p, miR-146a-5p, miR-223-3p and miR-200b-3p) was associated with severity of UC in pediatric patients and a combination of three miRNAs (miR-21-5p, miR-192-5p and miR-194-5p) was associated with early relapse of the disease. Nine patients out of the total were diagnosed with primary sclerosing cholangitis (PSC) simultaneously with ulcerative colitis. A panel of 6 candidate miRNAs (miR-142-3p, miR-146a-5p, miR-223-3p, let-7i-5p, miR-192-5p and miR-194-5p) identified those patients with PSC. Specific combinations of miRNAs are promising tools for potential use in precise disease identification and severity and prognostic stratification in pediatric patients with ulcerative pancolitis.

scholarly journals Prevalence of Erosive-ulcerative Lesions of the Stomach and Helicobacter Pylori Infection in Patients with Ulcerative Colitis

2019 ◽  
Vol 29 (3) ◽  
pp. 74-80
Author(s):  
N. A. Ponkratova ◽  
P. V. Pavlov ◽  
O. S. Shifrin
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Bowel Disease ◽  
Underlying Disease ◽  
Control Group ◽  
Inflammatory Bowel ◽  
Ulcerative Lesions ◽  
H Pylori

Aim. To assess the prevalence of erosive-ulcerative lesions of the gastric mucosa, as well as the frequency of Helicobacter pylori infection in patients with ulcerative colitis. Materials and methods. The study included 70 patients with ulcerative colitis. All patients, along with the standard examination using esophagogastroduodenoscopy, were diagnosed with regard to H. pylori infection: all 70 patients received a rapid urease test (RUT), 24 patients (34.3 %) had H. pylori DNA analyzed in feces, and 46 patients (65.7 %) had 13C labeled urea breath test. The study of H. pylori infection with RUT was carried out in 111 patients without inflammatory bowel disease, who formed the control group.Results. Endoscopic examination of patients with ulcerative colitis revealed inflammatory and erosive changes in the gastric mucosa. In 7.1 % of cases (5 out of 70 people), the erosion of the body stomach was detected, in 40.0 % of patients (28 out of 70) – erosion of the antrum. No effect of previous steroid therapy, as well as the high activity of ulcerative colitis on the frequency of the stomach erosive lesions (respectively, p = 0.433; p = 0.158) was detected. H. pylori infection was found in patients with ulcerative colitis significantly less frequently than in the control group (respectively, in 52.9 % (37 people out of 70) and 71.2 % of cases (79 people out of 111); p = 0.012). No relationship was found between H.pylori infection and the severity of the exacerbation of the underlying disease (p = 0.157).Conclusion. In patients with ulcerative colitis, erosive changes frequently found in the stomach do not correlate with the activity of the underlying disease. H. pylori infection is less common in patients with ulcerative colitis than in individuals not suffering from inflammatory bowel disease.

scholarly journals The condition of the oral cavity at the time of diagnosis of inflammatory bowel disease in pediatric patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Małgorzata Klichowska-Palonka ◽  
Aneta Komsta ◽  
Elżbieta Pac-Kożuchowska
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Oral Cavity ◽  
Oral Mucosa ◽  
Bowel Disease ◽  
Control Group ◽  
Systemic Diseases ◽  
Inflammatory Bowel

AbstractChanges in the oral mucosa can appear in the course of inflammatory bowel disease in both children and adults. They often precede the appearance of gastrointestinal symptoms. The aim of the study was to determine the nature of changes in the oral cavity at the time of diagnosis of inflammatory bowel disease in children compared to children without systemic diseases. 49 children diagnosed with inflammatory bowel disease and 60 children without systemic diseases were examined. The prevalence of the aphthae stomatitis and angular cheilitis was 24.5% in the examined group and 10% in the control group (p = 0.0772). Changes in the oral mucosa occurred more frequently in children with Crohn's disease 35.3% than with ulcerative colitis 18.7%. In children with Crohn's disease, the most frequently observed lesion was aphthous stomatitis 23.5%, and in ulcerative colitis, angular cheilitis 12.5%. Changes in the oral mucosa are a therapeutic problem requiring in general diseases patients both local and systemic treatment and interdisciplinary cooperation between dentists, paediatricians and gastroenterologists. The finding of repeated changes in the oral mucosa during a dental examination should be the reason for referring the patient to a paediatrician for the foreclosure or make a diagnosis of inflammatory bowel diseases.

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