scholarly journals Statistical Optimization of Culture Conditions for Protein Production by a Newly Isolated Morchella fluvialis

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Zahra Rahgo ◽  
Hamid reza Samadlouie ◽  
Shideh Mojerlou ◽  
Kambiz Jahanbin
Keyword(s):  
Amino Acids ◽  
Protein Production ◽  
Carbon Sources ◽  
Its Region ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Culture Conditions ◽  
Northern Region ◽  
Indispensable Amino Acids ◽  
Promising Source

Morchella fungi are considered a good source of protein. The ITS region was used to identify Morchella isolated in the northern region of Iran. The isolated fungus was very similar to Morchella fluvialis. M. fluvialis was first isolated in Iran. Dried biomass of M. fluvialis contained 9% lipids and 50% polysaccharides. Fatty acid profiles of lipids of M. fluvialis are mainly made up of linoleic acid (C18:2) (62%), followed by palmitic acid (C16:0) (12%). Testosterone (TS) was also detected (0.732 ng/dry weight biomass (DWB)) in the hormone profile of this new isolated species. Then, various protein and carbon sources as variable factors were applied to identify the key substrates, which stimulated protein production using the one-factor-at-a-time method. Key substrates (glucose and soybean) were statistically analyzed to determine the optimum content of the protein and DWB accumulation using response surface methods. The highest protein content (38% DWB) was obtained in the medium containing 80 g/l glucose and 40 g/l soybean powder. Total nutritionally indispensable amino acids and conditionally indispensable amino acids constitute 55.7% crude protein. That is to say, these adequate quantities of essential amino acids in the protein of M. fluvialis make it a good and promising source of essential amino acids for human diet.

Diversity of marine yeasts with high protein content and evaluation of their nutritive compositions

2008 ◽  
Vol 88 (7) ◽  
pp. 1347-1352 ◽  
Author(s):  
Zhenming Chi ◽  
Kuiran Yan ◽  
Lingmei Gao ◽  
Jing Li ◽  
Xianghong Wang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Nucleic Acid ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Marine Yeasts ◽  
Marine Animals ◽  
Hanseniaspora Uvarum ◽  
Yeast Strains ◽  
Routine Identification

A total of 327 yeast strains from seawater, sediments, mud of salterns, guts of the marine fish and marine algae were obtained. After crude protein of the yeasts was estimated by the method of Kjehldahl, we found that eight strains of the marine yeasts grown in the medium with 20 g l−1 glucose contained more than 30.4 g protein per 100 g of cell dry weight. The results of routine identification and molecular methods show that they belong to Metschnikowa reukaui, Cryptococcus aureus, Aureobasidium pullulan, Yarrowia lipolytica and Hanseniaspora uvarum, respectively. With the exception of Aureobasidium pullulans 4#2 with nucleic acid of 7.7% (w/w), all other yeast strains contained less than 5.0% (w/w) of nucleic acid. Analysis of fatty acids shows that all the yeast strains tested had a large amount of C18:0 and C18:1 fatty acids while analysis of amino acids indicates that the yeast strains tested had a large amount of essential amino acids, especially lysine and leucine which are very important nutritive components for marine animals.

Optimal levels of the essential amino acids histidine, lysine and threonine in Caenorhabditis elegans maintenance medium

Nematology ◽  
2008 ◽  
Vol 10 (4) ◽  
pp. 539-544 ◽  
Author(s):  
Nancy Lu ◽  
Xiaoyan Xiong
Keyword(s):  
Amino Acids ◽  
Caenorhabditis Elegans ◽  
Population Growth ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Maintenance Medium ◽  
C Elegans ◽  
Quantitative Studies ◽  
Free Living Nematode ◽  
Optimal Population

AbstractCurrent Caenorhabditis elegans Maintenance Medium (C-CeMM) is used to cultivate the free-living nematode, C. elegans. In C-CeMM, ten amino acids (AA) were found to be nutritionally essential. The optimal requirements of seven of these ten essential AA were determined previously. The objectives of the present study were to determine the optimal requirements of the remaining three essential AA: histidine, lysine and threonine. The Optimal Caenorhabditis elegans Maintenance Medium (O-CeMM) was formulated using the levels of all essential AA that supported the optimal population growth for C. elegans from previous quantitative studies and from the results obtained in the first part of this study. The efficacy of O-CeMM, C-CeMM, as well as Egg CeMM (E-CeMM), based on the essential AA ratio in hen's egg, was studied. The optimal requirements (mg ml–1) of histidine, lysine and threonine were determined to be 2.26 (8× C-CeMM), 1.03-2.06 (1× to 2× C-CeMM), and 0.717-2.86 (1× to 4× C-CeMM), respectively. It was found that O-CeMM supported a significantly higher (1.55 ∼ 1.60×) population growth (number of nematodes/ml) when compared with C-CeMM and E-CeMM. For both O-CeMM and C-CeMM, the AA efficiency ratio (AAER; g dry weight (wt) gain of C. elegans/g of total AA) was determined to be 0.18, which was significantly higher than the 0.15 that was determined in E-CeMM. Although the O-CeMM supported a significantly higher population growth, a higher level of histidine and consequently higher total AA were used in O-CeMM than in C-CeMM. Therefore, based on the findings on AAER, it was concluded that both the O-CeMM and C-CeMM were equally efficient for the cultivation of C. elegans.

scholarly journals A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2824
Author(s):  
Yixing Sui ◽  
Laura Mazzucchi ◽  
Parag Acharya ◽  
Yanan Xu ◽  
Geraint Morgan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Blue Light ◽  
Dunaliella Salina ◽  
Protein Quality ◽  
Monosodium Glutamate ◽  
Red Light ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Valuable Compounds ◽  
Β Carotene

Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene and amino acids in two strains cultivated under white, red or blue light until no further nitrogen was available. D. salina DF15 (CCAP 19/41 (PLY DF15)) produced more than 12% β-carotene (ash-free dry weight (AFDW) basis), and red light triggered the production of 9-cis β-carotene at a 9-cis/all-trans β-carotene ratio of 1.5. Phytoene production was also evident in D. salina DF15 under all conditions, particularly under blue light. However, the profile of essential amino acids (EAAs) and calculation of the essential amino acid index (EAAI) was less than ideal in terms of protein quality, for both strains. Umami compounds, quantified as monosodium glutamate (MSG) equivalents, indicated a higher equivalent umami concentration (EUC) in D. salina DF15 under red light (3.2 g MSG/100 g AFDW) than in D. salina CCAP19/30. Overall, D. salina DF15 demonstrates valuable traits for further exploration and product optimisation.

Nutritional profile of the wild harvested armoured cricket (Acanthoplus discoidalis) (Orthoptera: Tettigoniidae) in northern region of Zimbabwe

2021 ◽  
pp. 1-10
Author(s):  
A.K. Mugova ◽  
C.J. Zvidzai ◽  
R. Musundire
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
G Protein ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Daily Intake ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Nutritional Profile ◽  
Weight Content

Armoured crickets are an important food source in some parts of Zimbabwe. These insects are abundant in some regions but however, they are under utilised and not much is known about their nutritional value. The aim of this study was to determine the nutritional profile of the armoured cricket (Acanthoplus discoidalis). Armoured crickets were collected from a structured sampling framework in Mbire district Mashonaland Central province of Zimbabwe. Removal of the head and thorax, degutting and boiling were done. A proximate analysis was carried out followed by mineral, amino acid and fatty acid profiling of the prepared insects. Analyses were done on a dry basis. A. discoidalis contained 69.2% protein, 16.8% fat, 8.6% ash, 1.2% carbohydrate, 4.2% chitin and 454.3 Kcal/100 g energy. A 100 g dry weight quantity of insect contains 11.48 mg iron which is enough to meet the required daily intake of 10.0-20 mg/day for an adult. The zinc (4.37 mg/100 g dry weight) and phosphorus (491.4 mg/100 g dry weight) content in A. discoidalis almost meets the reference nutrient intake for adults. Essential amino acids, leucine (60.7 mg/g protein) had the highest concentration followed by phenylalanine+tyrosine (59.3 mg/g protein), valine (48.4 mg/g protein), lysine (46.7 mg/g protein), threonine (37.4 mg/g protein), isoleucine (26.4 mg/g protein), methionine+cysteine (20.9 mg/g protein) and histidine (16.5 mg/g protein) the least concentration. In total, 9 saturated fatty acids were determined of which stearic acid and palmitic acid had the highest concentrations of 2,034.5 mg/100 g and 2,005.3 mg/100 g respectively. Four mono unsaturated fatty acids were determined of which oleic acid and palmitoleic acid had the highest concentrations of 361.4 mg/100 g and 305.2 mg/100 g respectively. Four poly-unsaturated fatty acids were determined of which eicosapentaenoic and α-linolenic acid had the highest concentrations of 1,598.0 mg/100 g and 185.6 mg/100 g respectively. A. discoidalis is good source of protein, the minerals iron, zinc and phosphorus, some essential amino acids and poly-unsaturated fatty acids.

Intracellular free amino acid patterns in duodenal and colonic mucosa

1990 ◽  
Vol 36 (2) ◽  
pp. 378-381 ◽  
Author(s):  
G Ollenschläger ◽  
K Langer ◽  
H M Steffen ◽  
M Schrappe-Bächer ◽  
H Schmitt ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Medical Patients ◽  
The Mean ◽  
First Time ◽  
Duodenal Biopsies ◽  
Hiv Negative

Abstract We report for the first time the concentrations of free amino acids in human intestinal biopsies obtained by routinely performed endoscopy. We studied 15 medical patients with no changes of the mucosa and six HIV-infected persons with duodenitis. The mean (and SD) sum of all amino acids, taurine excepted, was 61.9 (5.4) mmol/kg dry weight in duodenal biopsies of HIV-negative subjects (n = 11) and 82.9 (0.6) mmol/kg in colonic specimens: 50% (44%) of the total (minus taurine) consisted of aspartate and glutamate and 14% (12%), of the essential amino acids. The relative amino acid pattern in duodenum and colon differed completely from that for muscle: aspartate was fourfold higher; glutamate, phenylalanine, glycine, valine, leucine, and isoleucine were about twofold higher. In contrast, glutamine amounted only to 4% (duodenum) to 14% (colon) of muscle glutamine. In duodenal biopsies of the HIV-infected persons, we found significantly (P less than 0.01, except glutamine: P less than 0.025) increased concentrations of glutamate (24.1 vs 17 mmol/kg dry weight), ornithine (1.4 vs 0.4), valine (2.2 vs 1.7), and glutamine.

scholarly journals AMINO ACID MIXTURES EFFECTIVE PARENTERALLY FOR LONG CONTINUED PLASMA PROTEIN PRODUCTION. CASEIN DIGESTS COMPARED

1944 ◽  
Vol 79 (6) ◽  
pp. 607-624 ◽  
Author(s):  
S. C. Madden ◽  
R. R. Woods ◽  
F. W. Shull ◽  
G. H. Whipple
Keyword(s):  
Amino Acids ◽  
Intravenous Injection ◽  
Plasma Protein ◽  
Nitrogen Balance ◽  
Protein Production ◽  
Nitrogen Retention ◽  
Essential Amino Acids ◽  
Skin Lesions ◽  
Good Utilization ◽  
Rapid Injection

When blood plasma proteins are depleted by bleeding with return of red cells suspended in saline (plasmapheresis) it is possible to bring dogs to a steady state of hypoproteinemia and a constant level of plasma protein production if the diet nitrogen intake is controlled and limited. Such dogs are outwardly normal but have a lowered resistance to infection and to certain intoxications. The ten growth essential amino acids of Rose plus glycine will maintain nitrogen balance and produce as much new plasma protein as will good diet proteins. This good utilization is demonstrated over periods of several months when the amino acids are given either orally or parenterally. There is no evidence of toxicity in general nor to unnatural forms of these synthetic amino acids in particular. Given parenterally appropriate mixtures of these amino acids are well tolerated even upon rapid injection. The minimal daily requirements for a 10 kilo dog may be given intravenously in 10 minutes without reaction. Subcutaneously a 10 per cent solution may be given rapidly without reaction. Among various mixtures tested Vt approximates a minimum for a 10 kilo dog. It contains in grams (dl-threonine 0.7, dl-valine 1.5, l-(-) leucine 1.5, dl-isoleucine 1.4, dl-lysine hydrochloride 1.5, l(-) tryptophane 0.4, dl-phenylalanine 1.0, dl-methionine 0.6, l(+)-histidine hydrochloride 0.5, l(+)-arginine hydrochloride 0.5, and glycine 1.0. The presence of glycine improves tolerance to rapid intravenous injection, but excess glycine does not improve utilization of the mixture. Over a long period this mixture appears suboptimal in quantity. Doubled it is more than ample. Of two casein digests tested the one prepared by enzymatic hydrolysis provided good nitrogen retention and fairly good plasma protein production but was much less tolerable upon intravenous injection than certain mixtures of pure amino acids. The other one prepared by acid hydrolysis and tryptophane fortification afforded bare nitrogen equilibrium and produced virtually no plasma protein. Skin lesions observed after 10 to 20 weeks of synthetic diet probably reflect a deficiency of some member or members of the vitamin B2 group. A persistent slight weight loss in the face of a strongly positive nitrogen balance may accompany this deficiency.

scholarly journals PLASMA PROTEIN PRODUCTION INFLUENCED BY AMINO ACID MIXTURES AND LACK OF ESSENTIAL AMINO ACIDS

1945 ◽  
Vol 82 (2) ◽  
pp. 77-92 ◽  
Author(s):  
S. C. Madden ◽  
F. W. Anderson ◽  
J. C. Donovan ◽  
G. H. Whipple
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Plasma Protein ◽  
Protein Production ◽  
Daily Intake ◽  
Essential Amino Acids ◽  
Nitrogen Excretion ◽  
Acid Mixture ◽  
Rapid Weight Loss ◽  
Urinary Nitrogen

When blood plasma proteins are depleted by bleeding with return of red cells suspended in saline (plasmapheresis) it is possible to bring dogs to a steady state of hypoproteinemia and a constant level of plasma protein production if the diet nitrogen intake is controlled and limited. Such dogs are outwardly normal but have a lowered resistance to infection and intoxication and probably to vitamin deficiency. When the diet nitrogen is provided by certain mixtures of the ten growth essential amino acids plus glycine, given intravenously at a rapid rate, plasma protein production is good. The same mixture absorbed subcutaneously at a slower rate may be slightly better utilized. Fed orally the same mixture is better utilized and associated with a lower urinary nitrogen excretion. An ample amino acid mixture for the daily intake of a 10 kilo dog may contain in grams dl-threonine 1.4, dl-valine 3, dl-leucine 3, dl-isoleucine 2, l(+)-lysine·HCl·H2O 2.2, dl-tryptophane 0.3, dl-phenylalanine 2, dl-methionine 1.2, l(+)-histidine·HCl·H2O 1, l(+)-arginine·HCl 1, and glycine 2. Half this quantity is inadequate and not improved by addition of a mixture of alanine, serine, norleucine, proline, hydroxyproline, and tyrosine totalling 1.4 gm. Aspartic acid appears to induce vomiting when added to a mixture of amino acids. The same response has been reported for glutamic acid (8). Omission from the intake of leucine or of leucine and isoleucine results in negative nitrogen balance and rapid weight loss but plasma protein production may be temporarily maintained. It is possible that leucine may be captured from red blood cell destruction. Tryptophane deficiency causes an abrupt decline in plasma protein production. No decline occurred during 2 weeks of histidine deficiency but the urinary nitrogen increased to negative balance. Plasma protein production may be impaired during conditions of dietary deficiency not related to the protein or amino acid intake. Skin lesions and liver function impairment are described. Unidentified factors present in liver and yeast appear to be involved.

scholarly journals Quantifying dietary macronutrient sources of carbon for bone collagen biosynthesis using natural abundance stable carbon isotope analysis

2006 ◽  
Vol 95 (6) ◽  
pp. 1055-1062 ◽  
Author(s):  
Susan Jim ◽  
Vicky Jones ◽  
Stanley H. Ambrose ◽  
Richard P. Evershed
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
De Novo ◽  
Stable Carbon Isotope ◽  
Isotopic Fractionation ◽  
Essential Amino Acids ◽  
Natural Abundance ◽  
Bone Collagen ◽  
Collagen Biosynthesis ◽  
Indispensable Amino Acids

The diets of laboratory rats were isotopically and nutritionally manipulated using purifiedC3 and/or C4 macronutrients to investigate the routing of dietary carbonto bone collagen biosynthesis. Diets were formulated with purified proteins, carbohydrates andlipids of defined composition and natural abundance stable isotope ratios. Bulk protein and constituent amino acid δ13C values determined for whole diet and bone collagen provided the basis for assessing isotopic fractionation and estimating the degree of routing versus synthesis de novo of essential, non-essential and conditionally indispensable amino acids. Essential and conditionally indispensable amino acids were shown to be routed from diet to collagen with little isotopic fractionation whereas non-essential amino acids differed by up to 20‰. Mathematical modelling of the relationships between macronutrient and tissue δ13C values provided qualitative and quantitative insights into the metabolic and energetic controls on bone collagen biosynthesis. Essential amino acids comprise 21·7% of the carbon in collagen, defining the minimum amount of dietary carbon routing. Estimates of 42 and 28% routing were shown for the non-essential amino acids, glycine and aspartate, respectively. In total, the routing of non-essential and conditionally indispensable amino acids was estimated to equal 29·6% of the carbon in collagen. When the contribution of carbon from the essential amino acids is also considered, we arrive at an overall minimum estimate of 51·3% routing of dietary amino acid carbon into bone collagen.

Cultivation of Eri-Silkworm Cordyceps militaris and Determination of its Amino Acid Composition

2013 ◽  
Vol 796 ◽  
pp. 21-24
Author(s):  
Yu Qiao Jin ◽  
Bing Li
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Large Scale ◽  
Essential Amino Acids ◽  
Culture Conditions ◽  
Cordyceps Militaris ◽  
Artificial Culture ◽  
Different Parts

In order to study eri-silkworm Cordyceps militaris culture condition and the amino acid composition features in different parts of it, this experiment optimized eri-silkworm Cordyceps militaris artificial culture conditions, and determined the amino acid composition in different parts. The results showed that the inoculated eri-silkworm Cordyceps militaris which was kept at low temperature (18 ° C) and seventeen dark days grew well and began to use natural light in the eighteenth day with humidity 85%, temperature 21 ° C, which could induce the germination of the sporophore. The artificial cultivation period was about 40 days. Amino acid test shows that its sporophore and stroma are rich in 17 kinds of amino acids, including 7 kinds of essential amino acids. The higher levels of amino acids in its sporophore are lysine 15.28%, glutamate 15.05% and aspartate 7.55%. The higher levels of the amino acids in the stroma are glycine 16.11%, lysine 9.16% and glutamate 8.30%. The results indicate that eri-silkworm Cordyceps militaris can be artificially cultured. As there are differences of amino acid composition in different parts of eri-silkworm Cordyceps militaris, this study lays the foundation for its large-scale cultivation and the exploitation of its amino acid nutrition.

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