scholarly journals Screening of Zoonotic Parasites in Playground Sandboxes of Public Parks from Subtropical Mexico

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Gonzalo A. Pacheco-Ortega ◽  
José I. Chan-Pérez ◽  
Antonio Ortega-Pacheco ◽  
Eugenia Guzmán-Marín ◽  
Melissa Edwards ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Toxocara Canis ◽  
Public Parks ◽  
Risk Of Infection ◽  
Ancylostoma Caninum ◽  
High Prevalence ◽  
Sag1 Gene ◽  
Zoonotic Parasites ◽  
The City ◽  
Southeastern Region

The pathological agents Toxoplasma gondii, Ancylostoma caninum, and Toxocara canis are widely distributed zoonotic parasites with high prevalence in tropical and subtropical regions of the world. The aim of the present study was to determine the presence of DNA from these parasites in sand samples from the sand playgrounds in the southeastern region of Mexico. Samples of sand were collected from 68 playgrounds in public parks in the city of Merida, Yucatan, which is the main urban area in the southeast of Mexico. The samples were examined using nested PCR to detect the SAG1 gene from Toxoplasma gondii, and endpoint PCR for the amplification of ITS-2 and rRNA-ITS2 genes from Toxocara canis and Ancylostoma caninum, respectively. The presence of T. gondii DNA was detected in 11.8% (8/68) samples, DNA from A. caninum and T. canis was not detected. Results indicate that playgrounds from the studied sandboxes are contaminated with T. gondii oocysts and may represent a risk of infection for people in contact with the sand, especially for preschoolers.

Prevalence of Toxoplasma gondii and Toxocara canis among Myositis Patients in Southwest of Iran

2019 ◽  
Vol 20 ◽  
Author(s):  
Jasem Saki ◽  
Karim Mowla ◽  
Reza Arjmand ◽  
Forough Kazemi ◽  
Somayeh Fallahizadeh
Keyword(s):  
Toxoplasma Gondii ◽  
Nested Pcr ◽  
Healthy Subjects ◽  
Toxocara Canis ◽  
Control Group ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
High Prevalence ◽  
Acute Toxoplasmosis ◽  
Southwest Of Iran

Introduction: Parasitic myositis is caused by some parasites such as T. gondii and T. canis. So, the aim of the study was to evaluate the prevalence T. gondii and T. canis in patients with myositis and healthy individuals. Methods: A total of 108 samples were randomly selected as the control (54 healthy individuals) and test (54 myositis patients) groups. IgG and IgM antibodies against T. gondii and IgG antibodies against T. canis were measured by the ELISA. The detection of chronic and acute toxoplasmosis was performed by the ELISA IgG avidity. The presence of T. gondii in blood was evaluated by the nested-PCR. Results: Of 108, 33 (30.6%) cases were detected positive for IgG against T. gondii that 19 (35.2%) and 14 (25.9%) were observed in myositis patients and healthy individuals, respectively (P=0.296). Of 19 positive cases, 12 (63.2%) and 7 (36.8%) cases were detected as chronic and acute toxoplasmosis, respectively, while, all positive cases in the control group had chronic toxoplasmosis (P=0.013). One (1.9%) sample was detected positive for anti- Toxoplasma gondii IgM and two (3.7%) samples were found positive for IgG against T. canis by the ELISA that these positive cases were observed only in myositis patients (P=1.000 P=0.495, respectively). B1 T. gondii gene was amplified in 12 (63.2%) and 1 (7.1%) in myositis patients and healthy subjects (P=0.001). Conclusions: Our findings showed that there was a relatively high prevalence of acute toxoplasmosis in myositis patients in comparison with the control subjects in southwest of Iran.

scholarly journals Surveillance of Zoonotic Parasites in Animals Involved in Animal-Assisted Interventions (AAIs)

2020 ◽  
Vol 17 (21) ◽  
pp. 7914
Author(s):  
Giulia Simonato ◽  
Patrizia Danesi ◽  
Antonio Frangipane di Regalbono ◽  
Giorgia Dotto ◽  
Cinzia Tessarin ◽  
...  
Keyword(s):  
Therapeutic Relationship ◽  
Potential Risk ◽  
Giardia Duodenalis ◽  
Toxocara Canis ◽  
Risk Of Infection ◽  
Positive Effects ◽  
Faecal Samples ◽  
Risk Of Exposure ◽  
Cutaneous Mycosis ◽  
Zoonotic Parasites

Animal-assisted interventions (AAIs) are based on the establishment of a therapeutic relationship between animals and beneficiaries that is certain to provide positive effects, while currently, it reads as if AAIs aim at exposing stakeholders to potential risk of infection. The surveillance of zoonotic pathogens is necessary for guaranteeing common health. This study investigated the presence of potentially zoonotic parasites, including dermatophytes, in animals involved in AAIs. Between 2015 and 2017, 190 animals (equids, dogs, cats, birds, rabbits, rodents, and goats) were investigated. Anamnestic and management data were recorded. Individual faecal samples were analysed using a copromicroscopic procedure. Fur and skin were examined for ectoparasites during clinical examinations, and samples for mycological investigation were collected by brushing. Parasites were described in 60 (31.6%) investigated animals. Thirteen out of the 60 (21.7%) animals harboured potentially zoonotic parasites, mainly recovered in dogs (Ancylostomatidae, Eucoleus aerophilus, Toxocara canis, and Giardia duodenalis) and a cat (G. duodenalis). Nannizzia gypsea and Paraphyton mirabile, potential agents of cutaneous mycosis, were isolated in a dog and a horse, respectively. No ectoparasites were found. AAIs might represent a source of infections either directly or via environmental contamination. Thus, active surveillance is necessary and animal screenings should be planned and scheduled according to the risk of exposure.

scholarly journals Detection and genotypes of Toxoplasma gondii DNA in feces of domestic cats in Colombia

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 25 ◽  
Author(s):  
Alejandro Zamora-Vélez ◽  
Jessica Triviño ◽  
Sebastián Cuadrado-Ríos ◽  
Fabiana Lora-Suarez ◽  
Jorge Enrique Gómez-Marín
Keyword(s):  
Toxoplasma Gondii ◽  
Optical Microscopy ◽  
Human Population ◽  
Phylogenetic Analyses ◽  
High Density ◽  
Domestic Cat ◽  
Fecal Samples ◽  
Stray Cats ◽  
High Prevalence ◽  
The City

The high prevalence of Toxoplasma gondii in the human population in Colombia has been linked to the existence of a high density of urban stray cats, exposing the whole population to a high density of oocysts. The goal of this study was to determine the DNA prevalence of T. gondii by conventional PCR and to phylogenetically analyze ROP18 sequences from positive samples in domestic cat (Felis catus) fecal samples in the city of Armenia, Quindío. Fecal samples from 140 cats were collected from 10 districts around the city. Samples were concentrated using Ritchie’s method and analyzed through optical microscopy. Concentrates were used for DNA extraction followed by nested PCR amplification for T. gondii gene B1. PCR for ROP18 was performed on all B1 positive samples; the ROP18 sequences obtained were related to the Archetype I Brazilian and Chinese strains. No oocysts were detected by optical microscopy; however, 17.8% (25/140) B1 and 24% (6/25) ROP18 PCR-positive samples were detected. Phylogenetic analyses showed that isolates clustered into a single group. We assessed whether associations existed between T. gondii positive fecal samples and survey variables such as cat healthcare and socioeconomic characteristics of owners, but no statistically significant associations were found. The presence of T. gondii in cat feces is an important factor contributing to the high prevalence in the human population of this city.

High prevalence of intestinal zoonotic parasites in dogs from Belgrade, Serbia — Short communication

2008 ◽  
Vol 56 (3) ◽  
pp. 335-340 ◽  
Author(s):  
Aleksandra Nikolić ◽  
Sanda Dimitrijević ◽  
Sofija Katić-Radivojević ◽  
Ivana Klun ◽  
Branko Bobić ◽  
...  
Keyword(s):  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Toxocara Canis ◽  
Stray Dogs ◽  
Significant Difference ◽  
High Prevalence ◽  
Working Dogs ◽  
Baseline Knowledge ◽  
Zoonotic Parasites ◽  
Military Working Dogs

To identify areas of risk for canine-related zoonoses in Serbia, the aim of this study was to provide baseline knowledge about intestinal parasites in 151 dogs (65 household pets, 75 stray and 11 military working dogs) from Belgrade. The following parasites, with their respective prevalences, were detected: Giardia duodenalis (14.6%), Ancylostomatidae (24.5%), Toxocara canis (30.5%), Trichuris vulpis (47.0%) and Taenia -type helminths (6.6%). Of all examined dogs, 75.5% (114/151) were found to harbour at least one parasite species. Of these, mixed infections with up to four species per dog occurred in 44.7% (51/114). Infections with all detected species were significantly higher (p < 0.05) in military working (100%) and stray dogs (93.3%) versus household pets (50.8%). Among all parasites, agents with zoonotic potential including Giardia , Ancylostomatidae and Toxocara were detected in 58.3% (88/151) of all examined dogs with a significant difference (p < 0.05) among the subgroups (100%, 62.7% and 46.2% for military working dogs, stray dogs and household pets, respectively). The high prevalence of zoonotic parasites registered in the dog population from a highly urban area in south-eastern Europe indicates a potential risk to human health. Thus, veterinarians should play an important role in helping to prevent or minimise zoonotic transmission.

scholarly journals Soil and sand contamination with canine intestinal parasite eggs as a risk factor for human health in public parks in Niš (Serbia)

2020 ◽  
Vol 57 (2) ◽  
pp. 109-119
Author(s):  
M. Ristić ◽  
N. Miladinović-Tasić ◽  
S. Dimitrijević ◽  
K. Nenadović ◽  
D. Bogunović ◽  
...  
Keyword(s):  
Human Health ◽  
Intestinal Parasites ◽  
Public Health Problem ◽  
Soil Samples ◽  
Variable Degree ◽  
Public Parks ◽  
Degree Of Contamination ◽  
Significant Difference ◽  
Zoonotic Parasites ◽  
The City

SummaryRegarding geographical distribution and clinical relevance, the most common canine geohelminths are Toxocara canis, ancylostomatids, and Trichuris vulpis. Canine intestinal parasites from the soil and sand present an important potential serious human health hazard, especially for the children preschool and school – age. This paper aimed to establish the degree of contamination of soil and sand with zoonotic parasites from the canine feces and the degree of risk they could pose for human health in public places and playgrounds in the city of Niš. Our parasitological study involved 200 soil samples and 50 sand samples from the public parks in the city of Niš in southeastern Serbia (43°19′15″N, 21°53′45″ E). From several locations, about 100 g of soil and sand was collected based on the bioclimatic indices. Parasitological diagnosis was performed using conventional qualitative and quantitative coprological methods, abiding by the recommendations about the diagnosis of parasitic diseases. In 38 – 46 % of soil samples and 40 % of sand samples seven species of endoparasites were diagnosed. In the samples of soil, a medium and high degree of contamination with the ascarid T. canis (14 – 22 %) was detected, as well as a low and medium degree of contamination with ancylostomatids (4 – 12 %), and in the samples of sand, a variable degree of contamination with the helminths T. canis (26 %) and A. alata (16 %) was found. A statistically significant difference was found in the contamination with A. alata eggs between the samples of sand and samples of soil. The studied public surfaces represent the reservoir of zoonotic parasites, which is a public health problem requiring a synergistic action of several factors to be successfully resolved, i.e. the implementation of prevention, surveillance, and control measures.

scholarly journals Zoonotic intestinal nematodes in dogs from public parks in Yucatán, México

Biomédica ◽  
2018 ◽  
Vol 38 (1) ◽  
pp. 105 ◽  
Author(s):  
Rodrigo Adán Medina-Pinto ◽  
Roger Iván Rodríguez-Vivas ◽  
Manuel Emilio Bolio-González
Keyword(s):  
Toxocara Canis ◽  
Public Parks ◽  
Ancylostoma Caninum ◽  
Trichuris Vulpis

Introducción. Los perros representan un potencial riesgo para la salud pública debido a que transmiten infecciones parasitarias al hombre.Objetivo. Estimar la frecuencia y determinar los factores asociados a la presencia de huevos de nematodos intestinales en heces de perros recolectadas en parques públicos de Mérida, Yucatán, México.Materiales y métodos. Se analizaron 100 muestras de heces de perros recolectadas en 20 parques públicos de dos zonas de la ciudad. Las muestras se procesaron mediante las técnicas de flotación centrifugada y de McMaster para confirmar la presencia de huevos de nematodos intestinales y cuantificarlos por gramo de heces. Se determinaron los factores asociados a la presencia de los huevos mediante un análisis univariado de χ2.Resultados. Se encontró una frecuencia de 11 %. Se identificaron huevos de tres especies de parásitos y Ancylostoma caninum fue el más frecuente (10 %), seguido por Toxocara canis (1 %) y Trichuris vulpis (1 %). La mayoría de las muestras positivas presentaba infección con un nematodo intestinal únicamente (10 %) y solo el 1 % resultó positivo para infección mixta por A. caninum y T. vulpis. La presencia de perros sin dueño en los parques públicos fue el factor asociado (p=0,046) con un mayor número de heces positivas para huevos de nematodos intestinales.Conclusiones. En los parques de la ciudad se encontraron heces de perros con huevos de nematodos intestinales con potencial zoonótico; la probabilidad de que las muestras fueran positivas fue mayor en los parques con presencia de perros sin dueño.

scholarly journals Prevalence of gastrointestinal parasites in domestic dogs in Tabasco, southeastern Mexico

2015 ◽  
Vol 24 (4) ◽  
pp. 432-437 ◽  
Author(s):  
Oswaldo Margarito Torres-Chablé ◽  
Ricardo Alfonso García-Herrera ◽  
Melchor Hernández-Hernández ◽  
Jorge Alonso Peralta-Torres ◽  
Nadia Florencia Ojeda-Robertos ◽  
...  
Keyword(s):  
Toxocara Canis ◽  
Gastrointestinal Parasites ◽  
Parasitic Infections ◽  
Parasitic Species ◽  
High Prevalence ◽  
Close Proximity ◽  
Public Areas ◽  
Study Population ◽  
The City ◽  
Trichuris Vulpis

Abstract The overall goal of this study was to estimate the prevalence of gastrointestinal (GI) parasites in dogs in the city of Villahermosa in Tabasco, Mexico. The study population consisted of 302 owned dogs that had limited access to public areas. A fecal sample was collected from each animal and examined for GI parasites by conventional macroscopic analysis and centrifugal flotation. Fecal samples from 80 (26.5%) dogs contained GI parasites. Of these, 58 (19.2%) were positive for helminths and 22 (7.3%) were positive for protozoan parasites. At least seven parasitic species were identified. The most common parasite was Ancylostoma caninum which was detected in 48 (15.9%) dogs. Other parasites detected on multiple occasions were Cystoisospora spp. (n = 19), Toxocara canis (n = 7) and Giardia spp. (n = 3). Three additional parasites, Dipylidium caninum, Trichuris vulpis and Uncinaria spp., were each detected in a single dog. No mixed parasitic infections were identified. In summary, we report a moderately high prevalence of GI parasites in owned dogs in Villahermosa, Tabasco. Several parasitic species identified in this study are recognized zoonotic pathogens which illustrates the important need to routinely monitor and treat dogs that live in close proximity to humans for parasitic infections.

Zoonotic parasites in dog feces from Leon, Mexico

2019 ◽  
Vol 29 ◽  
pp. 1-6
Author(s):  
Eduardo Alfonso Hernandez Muñoz ◽  
Jesús Martínez Canseco ◽  
Ariadna Beltrán-Rico ◽  
Roberto Hernández Tavera ◽  
Benjamín González Aguilera ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Intestinal Parasites ◽  
Fecal Contamination ◽  
Zoonotic Diseases ◽  
Public Parks ◽  
Fecal Samples ◽  
Concentration Technique ◽  
Zoonotic Parasites ◽  
The City ◽  
Common Parasite

Dogs are associated with at least 60 zoonotic diseases. Statistically, intestinal parasites stand out. The objective of this study is to evaluate the presence of zoonotic parasitic forms in dog feces collected from public parks and median strips in the city of Leon, México. Between June and August, 2015, dog feces were collected from 60 public parks and four median strips of the city. Samples were processed by the formalin-ether concentration technique and analyzed by optical microscopy. A total of 439 fecal samples from 64 areas were analyzed; the median of collected samples was 6 (Q1 to Q3: 2 to 12) per park. Contamination was present in 46 (71.88%) areas. It was identified that five (1.14%) areas tested positive for at least one parasite and one (0.23%) turned out positive for more than one parasite. The most common parasite was Ancylostoma spp, appearing in 3/439 samples (0.68%), among others. The rate of parks with fecal contamination was 71.88%, of which 10.87% showed parasitic contamination. Ancylostoma spp was the most prevalent parasite. Sanitary measures are needed to lower fecal contamination in public parks and the number of parasitized dogs.

Seroprevalence of Neospora caninum and Toxoplasma gondii Antibodies in the South American Opossum (Didelphis marsupialis) From the City of São Paulo, Brazil

2003 ◽  
Vol 89 (4) ◽  
pp. 870-871 ◽  
Author(s):  
L. E O. Yai ◽  
W. A. Cañon-Franco ◽  
V. C. Geraldi ◽  
M. E L. Summa ◽  
M. C G. O. Camargo ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Sao Paulo ◽  
South American ◽  
São Paulo ◽  
The South ◽  
American Opossum ◽  
The City

scholarly journals Parasitic contamination of urban and rural environments in the Slovak Republic: dog’s excrements as a source

2014 ◽  
Vol 51 (4) ◽  
pp. 273-280 ◽  
Author(s):  
I. Papajová ◽  
J. Pipiková ◽  
J. Papaj ◽  
A. Čižmár
Keyword(s):  
Slovak Republic ◽  
Intestinal Parasites ◽  
Toxocara Canis ◽  
Public Parks ◽  
Public Places ◽  
Rural Environments ◽  
Faecal Samples ◽  
One Year ◽  
Trichuris Vulpis ◽  
Toxascaris Leonina

AbstractThe objective of this study was to determine the possibility of soil contamination with propagative stages of intestinal endoparasites at different public places (public parks, playgrounds, sandpits, sidewalks, road sides). In a one-year-study, totally 578 dog’s faecal samples from 8 towns (Košice, Trebišov, Veľké Kapušany, Prešov, Snina, Levoča, Zvolen and Trenčín) and 3 villages (Dlhé Stráže, Dravce, Valaliky) were examined for the presence of parasitic germs. 29.9 % of faecal samples were positive. Eight different species of intestinal parasites with following incidence were detected: Toxocara canis (11.9 %), Trichuris vulpis (8.5 %), Ancylostomatidae (8.1 %), Taenia spp. (4.0 %), Toxascaris leonina (3.1 %), Capillaria spp. (1.0 %), Dipylidium caninum (0.2 %) and Coccidia oocysts (0.2 %). Additionally 285 sandpits were examined. Sand samples were collected from Košice (136), Zvolen (38), Trenčín (36), Prešov (30), Veľké Kapušany (24), Snina (15), and village Valaliky (6). The parasitic eggs occurence in the sandpits was as follows: Toxocara spp. (11.8 %), Ancylostomatidae (1.1 %), Taenia type (0.7 %) and Trichuris spp. (0.4 %). The occurrence of Toxocara spp. varied between the urban and rural environments. The highest number of Toxocara spp. was found in village Valaliky (33.3 %) whereas cities showed lesser prevalence (Prešov — 10 %, Košice — 7.4 %, Trenčín — 5.6 %, Zvolen — 5.3 %, Veľké Kapušany — 4.2 %). The epidemiological aspects and health risk factors are also discussed.

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