scholarly journals Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Linfen Xu ◽  
Lingna Huang ◽  
Chengying Lian ◽  
Huili Xue ◽  
Yanfang Lu ◽  
...  
Keyword(s):  
Pregnant Women ◽  
16S Rdna ◽  
High Throughput Sequencing ◽  
Interleukin 2 ◽  
Life Quality ◽  
Control Group ◽  
Operational Taxonomic Units ◽  
Causative Relationship ◽  
Rdna Sequencing ◽  
And Control

Embryonic miscarriage severely affects the life quality and physical and mental state of pregnant women. However, the detailed mechanism underlying embryonic miscarriage is not fully understood. This study is aimed at analyzing embryonic miscarriage. We collected samples from 25 normal pregnant women and 25 embryonic miscarriage patients of similar age to analyze microbiota isolated from the vagina. Crude examination of the vagina isolates showed that compared with the control group, 80% of the embryonic miscarriage group contained a significantly lower number of Lactobacillus, the major healthy microbe in the vagina. Furthermore, the levels of Th1 and Th2 secreted cytokine interleukin 2 (IL-2) and interleukin10 (IL-10), respectively, were examined. Results showed that the IL2 level was higher, and IL10 level was lower in the embryonic miscarriage group than in the control group, whereas the IL2/IL10 level was higher in the embryonic miscarriage group than in the control group. This finding suggested that the immune response was suppressed in the embryonic miscarriage group. To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed. Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively. At the genus level, the relative abundance of Fam_Finegoldia, Lac_Coprococcus_3, and Lac_Roseburia significantly differed in the embryonic miscarriage group. Overall, our analyses provided potential biomarkers for embryonic miscarriage and elucidated the causative relationship between microbiota and immune responses and may enable the possible diagnosis and therapeutics of early pregnancy loss.

scholarly journals Fate of Functional Bacterial and Eukaryotic Community Regulated by Earthworms during Vermicomposting of Dewatered Sludge, Studies Based on the 16S rDNA and 18S rDNA Sequencing of Active Cells

2021 ◽  
Vol 18 (18) ◽  
pp. 9713
Author(s):  
Jun Yang ◽  
Kui Huang ◽  
Lansheng Peng ◽  
Jianhui Li ◽  
Aozhan Liu
Keyword(s):  
16S Rdna ◽  
18S Rdna ◽  
High Throughput Sequencing ◽  
Propidium Monoazide ◽  
Eukaryotic Community ◽  
Rdna Sequencing ◽  
Stable Product ◽  
Active Bacteria ◽  
Dewatered Sludge ◽  
And Control

DNA sequencing of active cells involved in vermicomposting can clarify the roles of earthworms in regulating functional microorganisms. This study aimed to investigate the effect of earthworms on functional microbial communities in sludge by comparing biodegradation treatments with and without earthworms. PCR and high throughput sequencing based on pretreatment of propidium monoazide (PMA) were used to detect the changes in active bacterial 16S rDNA and eukaryotic 18S rDNA during vermicomposting. The results showed that the nitrate in sludge vermicomposting and control were significantly different from day 10, with a more stable product at day 30 of vermicomposting. Compared with the control, the Shannon indexes of active bacteria and eukaryotes decreased by 1.9% and 31.1%, respectively, in sludge vermicompost. Moreover, Proteobacteria (36.2%), Actinobacteria (25.6%), and eukaryotic Cryptomycota (80.3%) were activated in the sludge vermicompost. In contrast, the control had Proteobacteria (44.8%), Bacteroidetes (14.2%), Cryptomycota (50.00%), and Arthropoda (36.59%). Network analysis showed that environmental factors had different correlations between active bacterial and eukaryotic community structures. This study suggests that earthworms can decrease the diversity of bacterial and eukaryotic communities, forming a specific-functional microbial community and thus accelerating organic matter decomposition during vermicomposting of dewatered sludge.

scholarly journals Effect of Serum Copper on Circulating Angiogenesis-Related Factors in Women with Preeclampsia

2019 ◽  
Author(s):  
Khalid Najm Nadheer ◽  
Zohreh Zahraei ◽  
Hussein Al-Hakeim
Keyword(s):  
Pregnant Women ◽  
Serum Copper ◽  
Control Group ◽  
Vascular Endothelial ◽  
Related Factors ◽  
Soluble Endoglin ◽  
Iraqi Women ◽  
Endothelial Integrity ◽  
And Control ◽  
Endothelial Growth Factor

Preeclampsia (PE) is characterized by a series of clinical features such as hypertension and proteinuria associated with endothelial dysfunction and the impairment of placenta vascular endothelial integrity. This study aimed to investigate the effect of serum copper (Cu) level on some angiogenesis-related factors including vascular endothelial growth factor-A (VEGF-A), soluble Fms-like tyrosine kinase-1 (sVEGF-R1), soluble endoglin (sEng) and cerruloplasmin (Cp) in Iraqi women with preeclampsia (PE) and control pregnant women. Therefore, 60 women with PE in addition to 30 healthy pregnant women were enrolled in the study. Serum concentration of sEng, VEGF-A, sVEGF-R1, and Cu in PE group significantly increased (p<0.05) in the PE group compared with that in the control group. Increased production of antiangiogenic factors, soluble VEGF-A and sEng contribute to the pathophysiology of PE, indicating the involvement of these parameters in the angiogenic balance in patients with PE. Tests for between-subject effects showed that the circulating angiogenesis factors and Cu were significantly associated with the presence of PE. Serum Cu level was significantly correlated with VEGF- A and VEGF-R1 levels but not with sEng. Multiple regression analysis revealed that only Cp and BP can significantly predict the complications in women with PE. In conclusion, serum Cu has a role in the angiogenesis in women with PE and may be a new drug target in the prevention or treatment of PE.

scholarly journals Quality of Sleep and Quality of Life during Pregnancy

2020 ◽  
Vol 38 (3) ◽  
pp. 188-193
Author(s):  
Keun Tae Kim ◽  
Hyoeun Bae ◽  
Jin Gon Bae ◽  
Yong Won Cho
Keyword(s):  
Sleep Quality ◽  
Pregnant Women ◽  
Short Form ◽  
Life Quality ◽  
Control Group ◽  
Quality Of Sleep ◽  
Third Trimester ◽  
Cross Sectional ◽  
Sf 36

Background: The prevalence of sleep disorders increases as pregnancy progresses, which affects the health of pregnant women, fetal health, and the outcomes of pregnancy. The aim of this study is to evaluate the quality of sleep and life in pregnant women in Korea.Methods: This study is a prospective cross-sectional, case-control study of pregnant women and age-matched controls. From July to September 2019, all participants completed Korean-language versions of the sleep questionnaires including Pittsburgh sleep quality index (K-PSQI), Insomnia severity index, Epworth sleepiness scale, Back’s depression inventory-2(K-BDI), STOP (Snoring, Tiredness, Observed apneas, and high blood Pressure), and short-form 36 (K-SF-36).Results: A total of 422 participants consisted of 385 pregnant women and 137 controls. Second and third trimester were 200 and 185, respectively. K-PSQI scores were higher in pregnant women compared with the controls (7.87±3.49 and 8.50±3.55 vs. 5.79±2.76, <i>p</i><0.001). Total score of K-SF-36 was lowest in third trimester (62.07±17.72) and highest in the control group(79.41±13.36). There was no statistical difference between groups in K-BDI.Conclusions: This study demonstrated worsening of sleep quality as well as life quality during pregnancy. More attention to sleep of pregnant women is needed.

scholarly journals EFFECTIVENESS OF LAVENDER AROMATHERAPY AND CLASSICAL MUSIC THERAPY IN LOWERING BLOOD PRESSURE IN PREGNANT WOMEN WITH HYPERTENSION

2017 ◽  
Vol 3 (6) ◽  
pp. 750-756
Author(s):  
Sri Maisi ◽  
Suryono Suryono ◽  
Melyana Nurul Widyawati ◽  
Ari Suwondo ◽  
Suryati Kusworowulan
Keyword(s):  
Blood Pressure ◽  
Music Therapy ◽  
Systolic Blood Pressure ◽  
Pregnant Women ◽  
Classical Music ◽  
Combination Group ◽  
Control Group ◽  
P Value ◽  
The Mean ◽  
And Control

Background: Hypertension during pregnancy remains high in Indonesia. It is a major cause of maternal death. Aromatherapy lavender and classical music therapy are considered effective in lowering blood pressure in hypertension.Objective: To examine the effect of lavender aromatherapy and classical music therapy in lowering blood pressure in pregnant women with hypertension.Methods: A quasy experimental study with pretest-posttest control group design. There were 52 pregnant women with the inclusion criteria selected as samples using simple random sampling, divided into lavender aromatherapy group, classical music group, combination of aromatherapy and music group, and control group. Sphygmomanometer was used to measure blood pressure. Mann Whitney and Post Hoc test were used for data analysis.Results: Results showed that four groups have a significant decrease in systolic blood pressure after given intervention with p-value <0.05. The mean decrease of systolic blood pressure among four groups was: lavender group (5.77 mmHg), music group (7.23 mmHg), combination group (9.54 mmHg), and control group (3.67 mmHg); and the mean decrease of diastolic blood pressure was: the lavender group (2.77 mmHg), music group (0.61 mmHg), combination group (8.23 mmHg), and control group (3.42 mmHg).Conclusion: there was a significant effect of lavender aromatherapy and classical music therapy in lowering blood pressure in pregnant women with hypertension. However, the combination of both interventions was more effective than lavender aromatherapy or music therapy alone.

Investigating the Effect of Positive, Intermediate and Negative Enabling and Training Factors Affecting Physical Activity in Pregnant Women

2021 ◽  
Author(s):  
Leila Kianfard ◽  
Farkhonde Amin SHokravi ◽  
Sakineh Rakhshanderou ◽  
Shamsaddin Niknami
Keyword(s):  
Physical Activity ◽  
Pregnant Women ◽  
Control Group ◽  
Control Groups ◽  
Enabling Factors ◽  
Factors Affecting ◽  
Significant Difference ◽  
E Learning ◽  
And Control ◽  
Experimental Group

Abstract BackgroundPhysical activity during pregnancy period is one of the issues with priority during pregnancy period. Researches show that women reduce their physical activity during this period and are unaware of the benefits on the health of the mother and embryology. Although researchers regarding physical activity during pregnancy have prepared many guidelines; it is not however clear why pregnant women do not perform physical activity and the effectiveness factors that facilitate the desired behavior. MethodsThe research population included all pregnant women aged 18 to 40 years with gestational age of 12-38 weeks referring to the healthcare centers in District 5 of Tehran Municipality, from 22 districts of the city who had eliminated their physical activity during pregnancy. Questionnaires of physical activity assessment questionnaire after educational intervention in pregnant women (PPAQ) and a questionnaire designed based on the results of the needs assessment and the dimensions of the PEN-3 model were used for assessment. This study is a Research Clinical Trial (RCT). Due to the nature, a quasi-experimental research design (pre-test, post-test) with the control group was used in this research. ResultsBased on the obtained results, the intervention based on E-learning has a significant effect of enabling factors to increase physical activity in pregnant women in the experimental group. In addition, the intervention based on E-learning has a significant effect on the training factors in order to increase physical activity in pregnant women in the experimental group. ConclusionBased on the research results, it can be said that there is a statistically significant difference between the experimental and control groups in the scores obtained from training factors in the pretest and the experimental group. Furthermore, it can be said that there is a statistically significant difference between the experimental and control groups in the scores obtained from the enabling factors in the pre-test and the experimental group.

APC-RESISTANCE ASSOCIATED WITH FACTOR V LEIDEN GENE MUTATION (GENOTYPE GA): CLINICAL OCCURRENCE IN PREGNANCY

2018 ◽  
Author(s):  
М.Г. Николаева ◽  
А.П. Момот ◽  
Г.В. Сердюк ◽  
В.А. Елыкомов ◽  
К.А. Момот ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Gene Mutation ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Control Group ◽  
Factor V ◽  
Factor V Leiden Mutation ◽  
Apc Resistance ◽  
Factor Va ◽  
And Control

Цель исследования: изучить связь феномена резистентности фактора Vа к активированному протеину С (АПС-резистентность) при носительстве мутации гена FVL (1691) GA с клинической реализацией во время беременности тромботических событий и гестационных осложнений, таких как преэклампсия, задержка развития плода и невынашивание беременности. Материалы и методы. Проведено проспективное клиническое когортное исследование 1100 беременных. Выделено 2 когорты: основная группа – 500 пациенток с генотипом FVL (1691) GA и группа контроля – 600 женщин с генотипом FVL (1691) GG. Результаты. Медиана нормализованного отношения (НО) АПС-резистентности в контрольной группе у беременных с генотипом FVL (1691) GG колебалась в диапазоне 1,0→0,86. У беременных – носителей генотипа FVL (1691) GA этот показатель был достоверно ниже – 0,55→0,48 (р < 0,05). У пациенток при НО > 0,5 течение беременности было благоприятным. Более выраженная АПС-резистентность (НО ≤ 0,49) ассоциировалась с гестационными осложнениями. Заключение. Полученные данные по АПС-резистентности позволяют относить в группу высокого риска по тромботическим и акушерским осложнениям женщин – носительниц мутации фактора V Лейден (1691) не только с генотипом АА, но и с генотипом GA. AПС-резистентность ≤ 0,49 (по показателю НО) при носительстве мутации фактора V Лейден (1691) GA может рассматриваться как прогностический маркер развития гестационных осложнений с наибольшей точностью при сроке 7-8 недель беременности. Aim: to study during pregnancy the relationship between factor Va resistance to activated protein C (APC-resistance) in carriers of FVL gene mutation (1691) GA with clinical realization of thrombotic events and gestational complications such as preeclampsia, fetal growth retardation and miscarriage. Materials and methods. A prospective clinical cohort study of 1100 pregnant women was performed. Two cohorts were identified: main group – 500 patients with FVL genotype (1691) GA and control group – 600 women with FVL genotype (1691) GG. Results. The median of normalized ratio (NR) of APC resistance in the control group with FVL genotype (1691) GG ranged from 1.0→0.86. In pregnant women – the carriers of FVL genotype (1691) GA this parameter was significantly lower – 0.55→0.48 (р < 0.05). In patients with HO > 0.5 the course of pregnancy was favorable. More expressed APS-resistance (НО ≤ 0,49) was associated with gestational complications. Conclusion. The obtained data on APC-resistance allow to classify women – the carriers of Factor V Leiden (1691) mutation, not only with the AA genotype but also with GA genotype as the group of high risk for thrombotic and obstetric complications. APC resistance ≤ 0.49 (according NR) with the carriage of Factor V Leiden mutation (1691) GA can be considered as a prognostic marker for the development of gestational complications with the greatest accuracy at a period of 7-8 weeks of gestation.

scholarly journals Development of Late-Onset Preeclampsia in Association with Road Densities as a Proxy for Traffic-Related Air Pollution

2015 ◽  
Vol 39 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Meng Wu ◽  
Jean-Jacques Ries ◽  
Elena Proietti ◽  
Deborah Vogt ◽  
Sinuhe Hahn ◽  
...  
Keyword(s):  
Air Pollution ◽  
Pregnant Women ◽  
Late Onset ◽  
Epidemiological Studies ◽  
Control Group ◽  
Main Road ◽  
Increased Risk ◽  
Hypertensive Disorders In Pregnancy ◽  
And Control ◽  
Early Late

Introduction: Previous epidemiological studies indicate an association between maternal exposure to air pollution and an increased risk of hypertensive disorders in pregnancy. We analyzed the association between the occurrence of mild/severe and early-/late-onset preeclampsia (PE) and traffic-related air pollution (TRAP). Materials and Methods: Based on retrospective data, 50 pregnant women with PE were selected and matched with a control group of healthy pregnant women according to their age, parity, and number of fetuses. The total length of major roads around the women's home within a radius of 100, 200, 300, and 500 m and the distances from the domicile to the nearest ‘first class' main road and freeway were used as a proxy indicator of TRAP. We compared a PE subgroup and control group in terms of their exposure to TRAP. Results: Late-onset PE cases showed a significantly higher occurrence with density of major roads within a radius of 100-300 m compared to early onset cases (p = 0.006; 0.02; 0.04). In addition, a significantly shorter distance to the nearest ‘first class' main road was observed in late-onset PE cases (p = 0.0078). Conclusions: Exposure to TRAP during pregnancy was associated with an increased risk for the development of late-onset PE.

scholarly journals Seasonal Diversity of Endophytic Bacteria Associated with Cinnamomum camphora (L.) Presl.

Diversity ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 112 ◽  
Author(s):  
Abubakr Abdelwhab Hamd Elmagzob ◽  
Muhammed Mustapha Ibrahim ◽  
Guo-Fang Zhang
Keyword(s):  
16S Rdna ◽  
High Throughput Sequencing ◽  
Shannon Index ◽  
Ribosomal Database Project ◽  
Cinnamomum Camphora ◽  
Operational Taxonomic Units ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Endophytic Bacterial Community ◽  
Seasonal Diversity

Investigations on the density, diversity, and distribution of endophytic bacterial community associated with leaves of Cinnamomum camphora (L.) Presl. were carried out during three seasons using 16s rDNA high-throughput sequencing technology. Samples were collected from five species in Nanping (A, B, C, D, and E) and one from Fuqin (F) in the Fujian province, China in the months of April, July, and October (represented by 1, 2 and 3), indicating spring, summer, and early winter, respectively. Results from 16s rDNA sequences revealed 10,844,124 effective sequences. The highest OTUs (Operational taxonomic units) was highest in the A1 sample (1086), while the lowest was in C2 (509). Our observations showed that samples taken in October had the highest diversity of endophytes as indicated by the Shannon index (B3 = 5.3337), Chao1 (E3 = 1233.10), abundance-based coverage estimator (ACE) (A3 = 1764.72), and the Simpson indices of diversity (C3 = 0.1655) irrespective of the species. The order of the endophytes richness in the samples was April > July > October. The Ribosomal Database Project (RDP) classifier showed that the obtained sequences belonged to nine major phyla: Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Gemmatimonadetes, Acidobacteria, Planctomycetes, Chloroflexi, and Fusobacteria. Proteobacteria accounted for the highest proportion in each sample, ranging from 35.15% to 89.72%. These sequences belonged mainly to 10 orders: Rhizobiales, Clostridiales, Peseudomonadales, Burkholderiales, Bacteroidales, Enterobacteriales, Rhodocyclales, Sphingomonadales, Lactobacillales, and Bacillales. Also, other taxa with possible taxonomic statuses, which were unclassified, were present.

scholarly journals A Comparative Study of Relationship between Micronutrients and Gestational Diabetes

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Farideh Akhlaghi ◽  
Seyyed Majid Bagheri ◽  
Omid Rajabi
Keyword(s):  
Gestational Diabetes ◽  
Pregnant Women ◽  
Gestational Age ◽  
Control Group ◽  
Study Group ◽  
Inclusion Criteria ◽  
Control Groups ◽  
Significant Difference ◽  
Micronutrient Concentration ◽  
And Control

In this paper, we studied the relation between the micronutrient and gestational diabetes. Therefore, we measured micronutrient concentration including Ni, Al, Cr, Mg, Fe, Zn, Cu, and Se in serum of women with gestational diabetes between 24 and 28 weeks of gestational age (study group) who had inclusion criteria and comparison with micronutrient levels in normal pregnant women with same gestational age (control group). Results showed that there was no significant difference between the serum micronutrient level (Ni, Al, Cr, Mg, Zn, Cu, Se) in study and control groups except serum level of iron which in serum of gestational diabetic women was lower than normal pregnant women and difference was significant.

scholarly journals THE EFFECT OF PRENATAL GENTLE YOGA AND HYPNOTHERAPY ON ANXIETY LEVEL AND SLEEP QUALITY IN THE THIRD TRIMESTER PREGNANT WOMAN AT PUSKESMAS SUMBER CIREBON REGENCY IN 2019

2021 ◽  
Vol 1 (1) ◽  
pp. 32-38
Author(s):  
Nurlaela Kurnia Rahayu ◽  
Pepi Hapitria ◽  
Rani Widiyanti
Keyword(s):  
Sleep Quality ◽  
Pregnant Women ◽  
Intervention Group ◽  
Anxiety Level ◽  
Control Group ◽  
P Value ◽  
Third Trimester ◽  
Control Groups ◽  
The Third ◽  
And Control

As the pregnancy gets older, the attention and thoughts of pregnant women begin to focus on something that is considered as a climax, so that the anxiety experienced will intensify just before the childbirth. Excessive anxiety and sleep disorders during pregnancy can cause mental disorders in pregnant women and inhibit fetal growth. To determine the effect of prenatal gentle yoga and hypnotherapy on anxiety level and sleep quality in the third trimester pregnant women. This is a quasi-experimental study with a pre-post test with a control group. The sample was 32 respondents consisting of 16 respondents as an intervention group and 16 respondents as a control group taken by purposive sampling. The analysis used was the Paired T-test. There is a difference in the average of anxiety level in the third trimester pregnant women in the intervention and control groups with a p value 0,000; there is a difference in the average of sleep quality in the third trimester pregnant women in the intervention and control groups with a p value 0,000; and there is no difference in the average of anxiety level and sleep quality in the third trimester pregnant women in the intervention and control groups with a p value 0,64. Prenatal Gentle Yoga and Hypnotherapy have an effect in reducing the anxiety level and improving the sleep quality in the third trimester pregnant women.

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