scholarly journals Active Compound Identification in Extracts of N. lappaceum Peel and Evaluation of Antioxidant Capacity

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Mariel Monrroy ◽  
Onix Araúz ◽  
José Renán García
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Anthocyanin Content ◽  
Compound Identification ◽  
Total Anthocyanin ◽  
Food Industries ◽  
By Products

Nephelium lappaceum and its by-products have great potential in the agricultural, pharmaceutical, and food industries. Some studies have shown that N. lappaceum by-products exhibit antimicrobial, antioxidant, antidiabetic, and anticancer properties. However, studies focused on identifying these compounds are rare. The availability of polyphenolic compounds can vary according to environmental conditions, soil, plant variety, and agronomic management. Therefore, in this study, the active compounds in extracts of the N. lappaceum peel were identified, and their antioxidant properties were evaluated using various extraction solvents and both ultrasonic and boiling extraction techniques. The chemical characterization of the N. lappaceum peel exhibited carbohydrate and reducing sugar contents of 12 and 2%, respectively. Phytochemical analysis indicated the presence of flavonoids, tannins, terpenes, and steroids. The total phenolic and flavonoid contents and total antioxidant capacity were the highest in the hydroethanolic extract obtained by ultrasound, with values of 340 mg gallic acid equivalents g−1, 76 mg quercetin equivalents g−1, and 2.9 mmol of Trolox equivalents g−1, respectively. Contrarily, the total anthocyanin content was higher in the acid extract obtained by ultrasound, with a value of 0.7 mg cyanidin-3-O-glucoside equivalents g−1. A total of 18 compounds—including hydroxybenzene, phenolic acid, flavonoids, fatty acids (saturated, unsaturated, and ester), vitamin, arenecarbaldehyde, and phthalate—were identified for the first time in the N. lappaceum peel using gas chromatography-mass spectrometry. The identified compounds have been previously isolated from other plants and reportedly exhibit anticancer, anti-inflammatory, antimicrobial, and antioxidant activities. Thus, the N. lappaceum peel was shown to be a potential source of bioactive compounds of immense importance in the pharmacological and food industries.

scholarly journals Physicochemical and Bioactive Characteristics of Wild Grown Bilberry (Vaccinium myrtillus L.) Genotypes from Northeastern Turkey

2018 ◽  
Vol 46 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Ferit CELIK ◽  
Mehmet Ramazan BOZHUYUK ◽  
Sezai ERCISLI ◽  
Muttalip GUNDOGDU
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Fruit Weight ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Antioxidant Power

In present study, fruit weight, fruit firmness, external fruit color, soluble solid content, pH, titratable acidity, vitamin C, total phenolics, total anthocyanins, and antioxidant capacity of eight wild bilberry grown in Coruh valley in northeastern Turkey were determined. The blueberry cv. ‘Bluecrop’ is also included study to make comparison with bilberry genotypes. Antioxidant activity of bilberry and blueberry fruits were comparatively assessed by FRAP (Ferric reducing antioxidant power) assay. Significantly higher content of phenolics and anthocyanins was found in bilberry fruits than in blueberry fruits. However blueberry cv. ‘Bluecrop’ exhibited higher fruit weight and vitamin C content than bilberry fruits. Total phenolic and total anthocyanin content was 327 mg gallic acid equivalent and 142 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruit in cv. ‘Bluecrop’ while it was between 492-563 mg gallic acid equaivalent and 307-342 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruits of  bilberry accessions. Moreover, wild accessions approximately had 2-3 folds higher antioxidant capacity than cv. ‘Bluecrop’. Results suggest the possibility of improving the bioactive and antioxidant properties of bilberry cultivars based food products by using wild ones in cross breeding. It was also concluded that genotypes significantly affect their bioactive content and consequently the possibility of using wild bilberry fruits as a potential source of natural antioxidants in food industry.

scholarly journals Biocomponents and Antioxidant Activity of Ribes khorasanicum

2018 ◽  
Vol 3 (3) ◽  
pp. 99-103 ◽  
Author(s):  
Mohammad Ehsan Taghavizadeh Yazdi ◽  
Jalil Khara ◽  
Mohammad Reza Housaindokht ◽  
Hamid Reza Sadeghnia ◽  
Sedigheh Esmaeilzadeh Bahabadid ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sugar Content ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Bioactive Components ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Biological And Medical Applications

Currently, there have been lots of interests in phytochemicals as bioactive components. The roles of fruit, vegetables, and red pigments in preventing diseases have been partly accredited to the antioxidant properties of their constituent polyphenols, flavonoid, anthocyanins, and etc. Biochemistry parameters including the relative levels of antioxidant activity, total phenolic content, total flavonoid, total anthocyanin, soluble and insoluble sugar content of Ribes khorasanicum, have been calculated in this project. The total anthocyanin content of the investigated Ribes khorasanicum measured to be 62.9 mg/g in dry weight, while displaying high levels when compared to other components, which indicates that the anthocyanin content was the predominant antioxidant components in the investigated plant. This particular plant has the potential to serve as the important source of antioxidant that can be utilized in different biological and medical applications.

scholarly journals Effect of growing location on anthocyanin content and total antioxidant capacity of haskap (Lonicera caerulea L.) berry: A preliminary investigation

2021 ◽  
Vol 48 (No. 4) ◽  
pp. 183-189
Author(s):  
Aruma B.H.K. De Silva ◽  
H.P. Vasantha Rupasinghe
Keyword(s):  
Nova Scotia ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Ph Value ◽  
Preliminary Investigation ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Total Antioxidant

The haskap has been successfully cultivated in many geographical zones in Canada. We investigated whether the latitude has any impact on the anthocyanin accumulation and total antioxidant capacity in the haskap berry. The measured quality attributes were compared with that of the blueberry. Haskap ‘Aurora’ berries were collected from Yukon, British Columbia, Saskatchewan, and Nova Scotia in Canada, and blueberries were collected from Nova Scotia. Cyanidin-3-O-glucoside (C3G), the most abundant anthocyanin of haskap berry, the total anthocyanins, the total polyphenols, the soluble solids (°Brix), the pH value, the total antioxidant capacity, and the moisture content were determined. The results revealed that the total anthocyanin, total phenolic content, and antioxidant capacity of the haskap range between 88 to 273 mg C3GE/100 g fresh weight (FW), 256 to 442 mg GAE/100 g of FW, and 27 to 52 µmol TE/g FW, respectively. The liquid chromatography-mass spectrometry (UPLC/MS) analysis revealed that the C3G contained in the haskap berry is highly dependent on the harvest stage. Among the samples, the haskap berry harvested on July 19, 2019, from the Yukon had the highest C3G content. Interestingly, the total anthocyanin content of the haskap berry is comparable to that of the lowbush blueberry, but higher than the highbush blueberry. A systematic multi-year experiment employing many cultivars and growing locations is recommended to better understand the latitude effect.

scholarly journals Evaluation of The Physicochemical, Sensorial and Antioxidant Properties of Functional Ale Beer Brewed with Rice and Fruit by-Products

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Wichien Sriwichai ◽  
Pakkawat Detchewa ◽  
Patcharee Prasajak
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Principal Component ◽  
Banana Peel ◽  
Total Phenolic ◽  
Malt Extract ◽  
Flavonoid Content ◽  
Coffee Pulp ◽  
By Products ◽  
Rice Powder

Functional beer brewed with a high bioactive compound ingredients is beneficial to prevent many diseases. Rice and fruit by-products are rich in bioactive compounds and have a potential for functional beer production. The aim of the present work was to assess the physicochemical, nutritional and sensorial properties of the beer brewed with rice and fruit by-products. The five formulas of beer investigated were the dried malt extract, Phitsanulok paddy rice powder, riceberry powder, banana peel and coffee pulp. The ratio of the dried malt extract and the other alternative ingredients was 80:20. The contents in reducing sugar, total phenolic compounds, flavonoid, chlorogenic acid, caffeine and dietary fiber were evaluated. The correlations between the physicochemical, sensorial, antioxidant properties and acceptability of beers were assessed by the Principal Component Analysis. The results showed that beers were differed in color density and have a low alcohol content. Among beer formula, beers brewed with coffee pulp was the richest in total phenolic and beers brewed with banana peel was the richest in flavonoid content (278.82 mg gallic acid equivalent/ L beer and 69.18 mg catechin equivalent/ L respectively). The antioxidant capacity was the highest in banana peel beer formula (76.64%). The Principal Component Analysis revealed that beers brewed with the two varieties of rice powder formula were in the same group of beer control (dried malt extract formula). These beers were characterized by a high note of appreciation from consumer. Whereas, a high correlation between the antioxidant capacity and total polyphenolic, flavonoid content was observed in beer fruit by-products formulas. The incorporation of two varieties of rice powder or fruit by-products was feasible in brewing process to produce a functional ale beer appreciated by customer and having a noticeable antioxidant capacity.

scholarly journals Phenolics, antioxidative activity and characterization of anthocyanins in berries of blackcurrant interspecific hybrids.

1970 ◽  
Vol 60 (4) ◽  
Author(s):  
Nijolė Anisimovienė ◽  
Jurga Jankauskienė ◽  
Milda Jodinskienė ◽  
Vidmantas Bendokas ◽  
Vidmantas Stanys ◽  
...  
Keyword(s):  
Interspecific Hybrids ◽  
Antioxidative Activity ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Synthetic Antioxidant ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Berry Extracts

The interest in phenols and anthocyanins has increased due to their antioxidant properties and to their potential usage as dietary antioxidants in human nutrition. Total phenols and anthocyanin content, composition and stability in berry extracts of blackcurrant interspecific hybrids, and antioxidative activity of extracts was evaluated. Berries of interspecific hybrids accumulated 530 to 614 mg 100 g(-1) FW of total phenolic compounds, while 621 mg 100 g(-1) FW of phenolics was established in berries of control Ribes nigrum cultivar 'Ben Tirran'. 'Ben Tirran' berries accumulated 444 mg 100 g(-1) FW of anthocyanins and higher amount was identified in berries of interspecific hybrids No. 11-13 (R. nigrum × R. petraeum) and No. 57 (R. nigrum × R. aureum), 522 and 498 mg100 g(-1) FW respectively. Berry extracts of hybrid No. 11-13 distinguished by the highest antioxidative activity (80%) and it was higher than antioxidant activity of 'Ben Tirran' (70%). Antioxidative activity of all tested berry extracts (70-80%) was twice higher compared to synthetic antioxidant BHT (39%). However correlation between phenolics or total anthocyanin content and antioxidative activity degree was not established. Amount of cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside in berries of hybrids No. 57, No. 11-13 and No. 8 ((R. nigrum × R. americanum) × (R. nigrum × R. americanum)) was higher than in berries of 'Ben Tirran'. It was established that cyanidins are more stable anthocyanins in all studied temperature and irradiation conditions. Therefore interspecific hybrids No. 57 and No. 11-13 were the most agronomically valuable hybrids.

scholarly journals Antioxidant properties of Philippine Bignay (Antidesma bunius (Linn.) Spreng cv. ‘Common’) flesh and seeds as affected by fruit maturity and heat treatment

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 1980-1987
Author(s):  
K.A.T. Castillo-Israel ◽  
K.J.D. Sartagoda ◽  
M.C.R. Illano ◽  
L.E.L. Flandez ◽  
M.C.M. Compendio ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Antioxidant Content ◽  
Common Variety ◽  
Ripe Stage

The effects of maturity (green-unripe, red-ripe, and purple-fully ripe) and heat treatment (blanching at 90±5°C for 2 mins and steaming at 105±5°C for 5 mins) on the antioxidant content and activity of Philippine bignay (Antidesma bunius (Linn.) Spreng cv. ‘common’) flesh and seeds were investigated. The total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC) were determined using the Folin-Ciocalteau assay, aluminum chloride assay, and pH differential technique, respectively, and the antioxidant activity was determined using DPPH, ABTS, and FRAP assays. Results showed that the antioxidant content and activity of bignay increased with maturity. Fully ripe flesh and seeds were found to have the highest antioxidant content and activity regardless of the heat treatment applied. The TPC (8.06±0.15 mg gallic acid equivalents (GAE)/g), TFC (8.10±0.06 mg quercetin equivalents (QE)/g), TAC (4.07±0.03 mg cyanidin-3-glucoside equivalents (CGE)/L extract), and antioxidant activity (ABTS 10.37±0.04 , DPPH 9.60±0.10, FRAP 24.37±0.53 Trolox equivalents (TE)/g) of fully ripe seeds were superior when blanched. On the other hand, the TPC (40.73±0.04 mg GAE/g), TFC (31.54±0.15 mg QE/g), TAC (20.93±0.12 mg CGE/L extract), of fully ripe flesh were superior when not heat-treated while its antioxidant activity (ABTS 34.19±0.19, DPPH 14.01±0.02, FRAP 87.78±1.22 (TE)/g) was highest when blanched but did not vary significantly compared to unheated samples. This study suggested that bignay ‘common’ variety was a good source of antioxidants particularly the flesh at fully ripe stage. Blanching had shown to enhance its antioxidant activities.

scholarly journals Ultrasonic Effects on the Quality of Mulberry Juice

Beverages ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 56 ◽  
Author(s):  
Chi Nguyen ◽  
Ha Nguyen
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Mulberry Juice

This study was conducted to investigate the effects of ultrasonic treatments on the extraction yield and the quality of mulberry juice. The mulberry mash was treated with ultrasound at different incubation times from 30 to 120 min and different temperatures from 30 to 75 °C. The determination of the juice yield, total phenolic content, total anthocyanin content, antioxidant capacity, l-ascorbic acid content, total soluble solids, and the titratable acidity of the juice were carried out. Overall, applying ultrasound at 45 °C for 60 min resulted in the highest juice yield and antioxidant contents for the mulberry juice. The ultrasonic treatment increased the extraction yield (29.6%), the total soluble solid (8.7%), the titratable acidity (39.3%), the l-ascorbic acid content (94.3%), total phenolic content (174.1%), total anthocyanin content (156.9%) and the antioxidant capacity (40.7%) of the mulberry juice as compared to pressing only. A strong positive correlation between the total phenolic content and the antioxidant capacity indicated that phenolic compounds were the main antioxidants in the beverage.

scholarly journals Análisis de parámetros fisicoquímicos, compuestos fenólicos y capacidad antioxidante en piel, pulpa y fruto entero de cinco cultivares de manzana (Malus domestica) cosechadas en México//Analysis of physicochemical parameters, phenolic compounds and antioxidant capacity of peel, pulp and whole fruit of five apple varieties (Malus domestica) harvested in Mexico

Biotecnia ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 166-174
Author(s):  
Lizbeth Sandra Corona Leo ◽  
Diana Maylet Hernández-Martínez ◽  
Ofelia Gabriela Meza-Márquez
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Malus Domestica ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Positive Correlation ◽  
Apple Varieties ◽  
Dpph Method ◽  
Golden Delicious

La manzana es fuente de compuestos polifenólicos con propiedades antioxidantes que tienen efectos protectores para la salud humana. El objetivo del trabajo fue determinar los sólidos solubles totales (SST), acidez total (AT), pH, compuestos fenólicos totales (CFT), contenido total de flavonoides (CTF), contenido total de antocianinas (CTA) y capacidad antioxidante (ABTS y DPPH) en cinco variedades de manzana cultivadas en México. Los resultados indican que, en todas las variedades de manzana, los CFT, CTF, CTA, ABTS y DPPH se encuentran en mayor cantidad en la piel, seguido por el fruto entero y pulpa. En la manzana Golden delicious y en la pulpa de todos los cultivares no se detectaron antocianinas. El análisis de componentes principales (ACP) indica que los SST y AT están asociados a la pulpa y fruto entero, mientras que los CFT, CTF, CTA, ABTS y DPPH están asociados a la piel del fruto. Los CFT y CTA presentaron correlación positiva (r: 0.72-0.83) con el método DPPH en piel, mientras que el ensayo ABTS tuvo correlación positiva (r: 0.75-0.89) con CFT y CTF en piel. Los SST, AT, pH, CFT, CTF, CTA, ABTS y DPPH varían dependiendo del cultivar, región geográfica y parte del fruto.ABSTRACTApples are a source of polyphenolic compounds with antioxidant properties that have protective effects on human health. The objective of this work was to determine total soluble solids (SST), total acidity (AT), pH, total phenolic compounds (CFT), total flavonoid content (CTF), total anthocyanin content (CTA) and antioxidant capacity (ABTS and DPPH) of five apple varieties harvested in Mexico. Results indicate that CFT, CTF, CTA, ABTS and DPPH are distributed in the following order: peel, whole fruit, pulp. In the Golden delicious variety and in the pulp of all apple varieties no anthocyanins were detected. The ACP indicates that SST and AT are associated with pulp and whole fruit, while CFT, CTF, CTA, ABTS and DPPH are associated with the peel of the fruit. The CFT and CTA showed a positive correlation (r: 0.72-0.83) with the DPPH method in peel, while the ABTS assay had a positive correlation (r: 0.75-0.89) with CFT and CTF in peel. The SST, AT, pH, CFT, CTF, CTA, ABTS and DPPH vary depending on the cultivar, geographic region and part of the fruit.

scholarly journals Effect of Harvest Age on Total Phenolic, Total Anthocyanin Content, Bioactive Antioxidant Capacity and Antiproliferation of Black and White Glutinous Rice Sprouts

2020 ◽  
Vol 10 (20) ◽  
pp. 7051
Author(s):  
Visessakseth So ◽  
Piman Pocasap ◽  
Khaetthareeya Sutthanut ◽  
Benjabhorn Sethabouppha ◽  
Wipawee Thukhammee ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Antiproliferative Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Jurkat Cells ◽  
Biologically Active ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Glutinous Rice

Black (cv. BGR) and white (cv. RD6) glutinous rice sprouts from fertilizer- and pesticide-free farm in Khon Kaen province, Thailand were investigated for antioxidation and antiproliferative activity. Three different ages of rice sprouts were collected and prepared as the extract. BGR exerted higher antioxidant capacity than RD6 based on total phenolic (TPC) and total anthocyanin contents (TAC), DPPH, and FRAP assays. BGR at 10–15 days contained the highest TPC (29.72 ± 1.42 mg gallic acid equivalent/g extract) and reducing power (2.22 ± 0.014 mmole FeSO4/g extract). BGR at 20–25 days contained the highest TAC (0.86 ± 0.096 equivalence of cyanidin-3-glucoside/g extract) and DPPH radical scavenging activity (IC50 = 231.09 ± 12.99 μg/mL). Antiproliferative activity of the extracts was evaluated in the human T-lymphocyte (Jurkat), hepatocellular carcinoma (HepG2), colorectal carcinoma (HCT116), melanoma (SK-MEL-2) and noncancerous cells (Vero) by neutral red assay. BGR showed the most selective antiproliferation against Jurkat cells, by inducing apoptosis, and caspase 3/7 activity. BGR at 200 μg/mL from all ages significantly decreased ROS using DCFH-DA and increased endogenous glutathione levels in Jurkat cells compared to the control (p < 0.05). The higher antiproliferation of BGR than RD6 was via its antioxidation capacity and attributed to its higher phenolic and anthocyanin contents. BGR sprout is a potential source of biologically active substances good for wellness and health benefits.

scholarly journals Preservation of Antioxidant Properties of Endemic Dark Corn Using Solar Energy for Nixtamalization

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 401
Author(s):  
Hermelinda Servín-Campuzano ◽  
Mauricio González-Avilés ◽  
José Ángel Rodríguez-Morales ◽  
Marco Antonio Serrato Juárez ◽  
Jessica Grizzel Maya Castro ◽  
...  
Keyword(s):  
Solar Energy ◽  
Antioxidant Capacity ◽  
Difference Method ◽  
Antioxidant Properties ◽  
The Other ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Energy Process ◽  
Total Anthocyanin Content ◽  
Dpph Method

A comparative study of two corn nixtamalization processes is presented, one in the traditional way of the Michoacan region of Mexico, and the other using solar devices. The corn selected for the process was a nontransgenic endemic dark corn. For the nixtamalization process, a solar concentration oven was used; this process using the solar device is simple and affordable for communities. For characterization, the properties of the antioxidant content of dark corn were analyzed for both processes, and the highest concentration of antioxidants between the two varieties of corn was obtained. The antioxidant capacity in the two varieties of black corn was quantified and determined, and the anthocyanin pigments were extracted with methanol. The total anthocyanin content (CAT) was evaluated by the pH difference method, and the antioxidant capacity by the DPPH method. In the solar nixtamalization process, a higher content of CAT anthocyanins and antioxidant capacity were obtained. Therefore, the solar energy process was proposed as a sustainable energy option, and we concluded that black corn nixtamalized with a solar oven had high antioxidant levels and could be used as a nutritious food.

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