scholarly journals Antimicrobial Evaluation of Latex and TLC Fractions from the Leaves of Aloe adigratana Reynolds

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Demoze Asmerom ◽  
Gebremedhin Solomon Hailu ◽  
Ebrahim M. Yimer ◽  
Helen Bitew ◽  
Getu Kahsay
Keyword(s):  
Staphylococcus Aureus ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antimicrobial Agents ◽  
Antimicrobial Effect ◽  
Candida Krusei ◽  
Assay Method ◽  
Bacterial Strains ◽  
Microbial Strains ◽  
Layer Chromatography

Background. The highest prevalence and emergence of microbial infections coupled with the threat of antimicrobial resistance constitute a global concern, which entails searching for novel antimicrobial agents. Medicinal plants are among the major sources of medicines for novel drug discovery. Aloe adigratana is one of the endemic Aloe species in Ethiopia where the leaf latex of the plant is traditionally used for the treatment of various pathogenic conditions such as wound, dandruff, malaria, and diabetes. In spite of such claims, there was no scientific study done so far. The aim of the current study was, therefore, to evaluate the antimicrobial effect of leaf latex of A. adigratana and its thin layer chromatography (TLC) fractions. Methods. Thin layer chromatography (TLC) separation was employed for isolation of bioactive compounds. Agar well diffusion and microdilution assay method were used to evaluate the antimicrobial actions of the leaf latex and TLC fractions against six bacterial strains and four Candida species of reference and clinical isolate microbial strains. Results. Three major fractions, AA01, AA02, and AA03, were identified by TLC. Among the tested microbial strains, the reference strain of Staphylococcus aureus ATCC 29213 (MIC = 0.06 mg/mL) and clinical Candida krusei 242/18 (MIC = 0.14 mg/mL) exhibited higher susceptibility towards AA02, while reference strains of Klebsiella pneumoniae ATCC 700603 (MIC = 0.19 mg/mL) revealed the highest susceptibility towards AA01. The leaf latex displayed the highest activity against Staphylococcus aureus ATCC 29213 and clinical Candida krusei 242/18 with a MIC value of 0.19 mg/mL. Conclusion. The leaf latex and TLC fractions were found to be active against the tested bacterial and Candida species. Therefore, this finding supports the traditional claim of Aloe adigratana and the need for characterization of the TLC fractions to provide as lead compounds for further comprehensive antibacterial and antifungal activities.

scholarly journals ANTIBACTERIAL ACTIVITY AND THIN LAYER CHROMATOGRAPHY PROFILE Muntingia Calabura LEAF EXTRACT AS A MATERIAL CANDIDATE HAND SANITIZER

2018 ◽  
Vol 11 (1) ◽  
pp. 43
Author(s):  
Santy Pristianingrum ◽  
Baiq Lely Zainiati ◽  
Iwan Doddy Dharmawibawa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Preventive Action ◽  
Hand Sanitizer ◽  
Layer Chromatography ◽  
Active Substances ◽  
Muntingia Calabura

Abstract : the utilization of antibacterial active substances from several plants is increasingly not only as the ingredients of medicine, but its utilization is also utilized for an antibacterial ingredient for preventive action, one of them is hand sanitizer material. This research focused to find the antibacterial active substances alternative from Muntingia calabura leaves extract. The data were analyzed descriptively including the inhibitory of ethanol absolute extract and ethanol 95% of M. Calabura against isolate clinical bacteria by Kirby Bauer method and the type of coumpund that contains in M.calabura leaf by thin layer chromatography utilizingeluen n-hexan- Methanol. Etanol absolute extract M.calabura leaf showing the average inhibition zone against Pseudomonas aeruginosa 15.67 mm, Staphylococcus aureus 19.33 mm and Escherichia coli 13 mm. While, The etanol extract 95% showing higher inhibition Pseudomonas aeruginosa 19.67 mm, Staphylococcus aureus 19.33 mm and Escherichia coli 16.67 mm. This inhibitory zone was slightly lower than chlorhexidine gluconate with an average of 20-24 mm against the three bacteria that utilized in the test but belongs to the strongly sensitive category for natural materials according to Mukherjee (1988). From thin layer chromatography profile with eluen n-hexan: methanol found three compounds that were in the range of Rf value 0.4; 0.5 and 0.7. The Conclusion for this study is the bioactive material from etanol 95% extract M.calabura leaf can be optimized to the hand sanitizer active compound candidate.

A Rapid and Inexpensive Method for the Determination of Biogenic Amines from Bacterial Cultures by Thin-Layer Chromatography

2005 ◽  
Vol 68 (3) ◽  
pp. 625-629 ◽  
Author(s):  
EMILIA GARCÍA-MORUNO ◽  
ALFONSO V. CARRASCOSA ◽  
ROSARIO MUÑOZ
Keyword(s):  
Thin Layer ◽  
Biogenic Amines ◽  
Thin Layer Chromatography ◽  
Culture Media ◽  
Bacterial Strains ◽  
Inexpensive Method ◽  
Ph Indicator ◽  
Amino Acid Precursor ◽  
Differential Medium ◽  
Layer Chromatography

This study describes a simple, rapid, and inexpensive method to determine the ability to produce biogenic amines (BA) by bacteria in liquid culture media containing the corresponding amino acid precursor. In view of their role as starters in food fermentation, BA formation by these microorganisms has to be taken into consideration by selecting appropriate strains. For the standardization of the assay pure BA were mixed. The method avoids a prior extraction step of the amines and allows the separation and identification of the amines histamine, tyramine, putrescine, and phenylethylamine using thin-layer chromatography. The method was successfully applied to several BA-producer bacterial strains. This method constitutes a simple solution to the previous reports describing false-positive reactions in routine screening procedures generally involving the use of a differential medium containing a pH indicator.

scholarly journals Studies on the Antibacterial Activity and Chemical Composition of Methanol Extract of Cochlospermum tinctorium Root

2019 ◽  
pp. 1-11
Author(s):  
R. Abdulaziz ◽  
M. H. Usman ◽  
U. B. Ibrahim ◽  
B. M. Tambari ◽  
A. Nafiu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Plant Extract ◽  
Methanol Extract ◽  
Antibiotic Resistant ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Layer Chromatography

The aim of study was to evaluate the antibacterial activity of Cochlospermum tinctorium against ten (10) strains of antibiotic resistant food-borne pathogens Staphylococcus aureus and Listeria monocytogene. Ten (10) strains of antibiotic resistant food-borne pathogens Staphylococcus aureus and Listeria monocytogene procured from Microbiology Research Laboratory Usman Danfodiyo University Sokoto. The roots of Cochlospermum tinctorium were collected from the rock side in Dambu Gomo, Zuru Local Government Area of Kebbi State, Nigeria. The roots were washed, air-dried and milled to powder using mortal and pestle and sieved to obtained fine powder. Maceration was used for extraction using methanol as solvent. The antibacterial activity of the plant was determined on Mueller Hinton agar using agar well diffusion method. Minimum concentration (MIC) and minimum inhibitory concentration (MBC) of plant extract was also determined. Thin layer chromatography and column chromatography was employed for separation and fraction of different compounds in the plant extract. The fractions were screened for antibacterial activity and active fractions having high antibacterial activity were subjected Gas Chromatography Mass Spectoscopy (GC-MS) analysis. The result of methanol extraction yield 5.17% extracts. The methanol extract of Cochlospermum tinctorium was effective in inhibiting the isolates at high concentration of 10 mg/mL. The results thin layer chromatography revealed four spots with Rf values 0.02, 0.37, 0.44 and 0.80 respectively. The GC-MS analysis of the active methanol extract of Cochlospermum tinctorium root powder revealed the existence of major peaks 1-(+)-Ascorbic acid 2,6-dihexadecanoate (R.T: 13.666), Diethyl phthalate (R.T: 10.440), Undecyl acetate (R.T: 10.007), 3-tetradecanone (R.T: 9.793), 3-hexadecanone (R.T: 12.427). It therefore concluded that the root of Cochlospermum tinctorium has immense potential to be used in the area of pharmacology as it possess antimicrobial activity against the antibiotic resistant food-borne pathogens, thus could be exploited as alternative antimicrobial drugs.

scholarly journals Cytotoxic Activity of Flavonoid Compounds Isolated from Tetrameles Nudliflora Leaves

2020 ◽  
Vol 23 (2) ◽  
pp. 81
Author(s):  
Megawati Megawati ◽  
Akhmad Darmawan ◽  
Sofa Fajriah
Keyword(s):  
Thin Layer ◽  
Cytotoxic Activity ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Assay Method ◽  
Cytotoxic Activities ◽  
Spectroscopic Techniques ◽  
Flavonoid Compounds ◽  
Layer Chromatography ◽  
Mcf 7

Tetrameles nudliflora is one of the endemic plants in Wallace region, especially in Mekongga Forest, Southeast Sulawesi, Indonesia. As part of our project in the field of natural drug discovery based on local bioresources, this study aimed to isolate secondary metabolite compounds from the ethyl acetate fractions of the leaves of T. nudiflora and evaluate their cytotoxic activities against breast carcinoma cancer lines, MCF-7. The methods used were various chromatography techniques such as vacuum column chromatography, gravitation column chromatography, thin layer chromatography, preparative thin layer chromatography, and re-crystallization using two different organic solvents. In the present study, two flavonoid compounds, kaempferol (1) and quercetin (2) were identified. The structures of 1-2 were determined using serial spectroscopic techniques, including mass spectrometry, one- and two-dimensional nuclear magnetic resonance. Moreover, using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay method, both compounds showed in vitro cytotoxic activity IC50 of 34.162 and 133.148 μg mL-1, respectively against MCF-7 cell lines.

scholarly journals Synthesis of quaternary ammonium salts based on quinuclidin-3-ol and pyridine-4-aldoxime with alkyl chains

St open ◽  
2020 ◽  
Vol 1 ◽  
pp. 1-8
Author(s):  
Doris Crnčević ◽  
Renata Odžak
Keyword(s):  
Melting Point ◽  
Nitrogen Atom ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antimicrobial Agents ◽  
Ftir Spectra ◽  
Ammonium Salts ◽  
Product Yields ◽  
Alkyl Chains ◽  
Layer Chromatography

Aim: In search of a new class of potential antimicrobial agents, novel ammonium salts based on quinuclidine-3-ol and pyridine-4-aldoxime with different lengths of alkyl chains were synthesized and analyzed. In addition to their potential biological application, newly synthesized salts containing terminal bromine could possibly be used for the synthesis of more potent bisquaternary salts. Methods: The commercially available quinuclidine-3-ol and pyridine-4-aldoxime were used for the synthesis of new derivatives with the appropriate alkyl chains. The purity of the synthesized salts was tested by thin-layer chromatography on an aluminum plate where aluminum oxide was used as a stationary phase. Melting points were determined in open capillaries using an instrument for melting point determination and the obtained values were left uncorrected. The FTIR spectra were recorded with a spectrometer and the data were analyzed in cm-1. Results: All the synthesized compounds, which contained heterocyclic moiety and alkyl chains (with or without a terminal bromine atom), were obtained in very good yields under the simple quaternization conditions. The better product yields were observed in reactions with quinuclidine3-ol (46-95%) compared to those with pyridine-4-aldoxime (49-59%). The obtained products were analyzed and confirmed by the thin-layer chromatography, the melting point measurement and the FTIR spectra. Conclusion: The observed difference in product yields could be explained by the different basicity of the nitrogen atom of quinuclidin-3-ol than that of pyridine-4-aldoxime. All prepared salts have a positively charged nitrogen atom as a part of their polar “head” and a long hydrocarbon chain as the non-polar “tail”. Such a structure allows electrostatic interaction with the negatively charged bacterial membrane and causes it to be disrupted. The structure of the prepared salts containing long alkyl chains could be crucial for their antimicrobial activity.

scholarly journals Short tryptophan- and arginine-rich peptide shows efficacy against clinical methicillin-resistant Staphylococcus aureus strains isolated from skin and soft tissue infections

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mihaela Bacalum ◽  
Elena-Carmina Dragulescu ◽  
George Necula ◽  
Irina Codita ◽  
Mihai Radu
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Antimicrobial Agents ◽  
Therapeutic Potential ◽  
Antimicrobial Effect ◽  
Soft Tissue Infections ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
Membrane Level

AbstractIn recent years methicillin-resistant Staphylococcus aureus has posed a challenge in treating skin and soft tissue infections. Finding new antimicrobial agents has therefore become imperative. We evaluated the in vitro antimicrobial activity of a synthetic peptide, P6, against multidrug resistant clinical strains of Staphylococcus aureus isolated from skin and soft tissue infections. The P6 antimicrobial effect was evaluated in vitro by determining MIC/MBC, the ratio of live/dead cells and the effects induced at membrane level. The therapeutic efficiency was determined against human skin cells. P6 inhibited growth for all strains between 8 and 16 mg/L and killed all bacterial strains at 16 mg/L. The therapeutic potential was found to be 30 and 15 in the presence of BSA. We showed that P6 localizes at membrane level, where it acts slowly, by depolarizing it and affecting its integrity. P6 can be considered a good candidate for use as an antimicrobial agent in topical applications.

Sign in / Sign up

Export Citation Format

Share Document