scholarly journals Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Pavel Borsky ◽  
Zdenek Fiala ◽  
Ctirad Andrys ◽  
Martin Beranek ◽  
Kvetoslava Hamakova ◽  
...  
Keyword(s):  
Disease Severity ◽  
Significant Relationship ◽  
Psoriasis Vulgaris ◽  
Serum Levels ◽  
Intracellular Proteins ◽  
Elisa Technique ◽  
Commercial Kits ◽  
Destructive Processes ◽  
Chronic Autoimmune Disease ◽  
The Relationship

Background. Psoriasis vulgaris is a chronic autoimmune disease associated with systemic inflammation. Increased levels of numerous cytokines, chemokines, growth factors, and other molecules were found in the skin and in the circulation of psoriatic patients. Alarmins, also known as danger signals, are intracellular proteins, which are released to an extracellular space after infection or damage. They are the markers of cell destructive processes. Objective. The aim of the present study was to evaluate the suitability of selected alarmins (HMGB1, IL-33, S100A7, and S100A12) as potential biomarkers of severity of psoriasis and to explore possible relationships between these proteins for the purpose of better understanding their roles in the immunopathology of psoriasis. Methods. The serum levels of selected alarmins were measured in 63 psoriatic patients and 95 control individuals. The levels were assessed by the ELISA technique using commercial kits. The data were statistically processed with MedCalc version 19.0.5. Results. In psoriatic patients, we found significantly increased levels of HMGB1 (p<0.05), IL-33 (p<0.01), S100A7 (p<0.0001), and S100A12 (p<0.0001). In addition, we found a significant relationship between HMGB1 and S100A7 (Spearman’s rho=0.276, p<0.05) in the patients and significant relationship between HMGB1 and IL-33 in the controls (Spearman’s rho=0.416, p<0.05). We did not find any relationship between observed alarmins and the disease severity. Conclusions. The alarmins HMGB1, IL-33, S100A7, and S100A12 were significantly elevated in the serum of patients, which states the hypothesis that they play specific roles in the immunopathology of psoriasis. However, we have not yet found a relationship between observed alarmins and the disease severity. The discovery of the relationship between HMGB1 and S100A7 is a novelty that should be studied in the future to further clarify its role and importance.

scholarly journals The Relationship Between Serum Levels of Total IgE, IL-18, IL-12, IFN-γ and Disease Severity in Children With Atopic Dermatitis

2006 ◽  
Vol 2006 ◽  
pp. 1-4 ◽  
Author(s):  
Murat Aral ◽  
Ozer Arican ◽  
Mustafa Gul ◽  
Sezai Sasmaz ◽  
Sumeyra Alkis Kocturk ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Disease Severity ◽  
Serum Levels ◽  
Total Ige ◽  
Ifn Γ ◽  
The Relationship

Immunoinflammatory Biomarkers in Serum Are Associated with Disease Severity in Atopic Dermatitis

Dermatology ◽  
2021 ◽  
pp. 1-8
Author(s):  
Jesper Grønlund Holm ◽  
Guillem Hurault ◽  
Tove Agner ◽  
Maja Lisa Clausen ◽  
Sanja Kezic ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Disease Severity ◽  
Predictive Models ◽  
Severe Disease ◽  
Serum Levels ◽  
Disease Characteristics ◽  
Immunological Markers ◽  
Chemokine Ligand ◽  
Serum Total Ige ◽  
The Relationship

Background: A growing body of evidence links various biomarkers to atopic dermatitis (AD). Still, little is known about the association of specific biomarkers to disease characteristics and severity in AD. Objective: To explore the relationship between various immunological markers in the serum and disease severity in a hospital cohort of AD patients. Methods: Outpatients with AD referred to the Department of Dermatology, Bispebjerg Hospital, Copenhagen, Denmark, were divided into groups based on disease severity (SCORAD). Serum levels of a preselected panel of immunoinflammatory biomarkers were tested for association with disease characteristics. Two machine learning models were developed to predict SCORAD from the measured biomarkers. Results: A total of 160 patients with AD were included; 53 (33.1%) with mild, 73 (45.6%) with moderate, and 34 (21.3%) with severe disease. Mean age was 29.2 years (range 6–70 years) and 84 (52.5%) were females. Numerous biomarkers showed a statistically significant correlation with SCORAD, with the strongest correlations seen for CCL17/thymus and activation-regulated chemokine (chemokine ligand-17/TARC) and CCL27/cutaneous T cell-attracting-chemokine (CTACK; Spearman R of 0.50 and 0.43, respectively, p < 0.001). Extrinsic AD patients were more likely to have higher mean SCORAD (p < 0.001), CCL17 (p < 0.001), CCL26/eotaxin-3 (p < 0.001), and eosinophil count (p < 0.001) than intrinsic AD patients. Predictive models for SCORAD identified CCL17, CCL27, serum total IgE, IL-33, and IL-5 as the most important predictors for SCORAD, but with weaker associations than single cytokines. Conclusions: Specific immunoinflammatory biomarkers in the serum, mainly of the Th2 pathway, are correlated with disease severity in patients with AD. Predictive models identified biomarkers associated with disease severity but this finding warrants further investigation.

Evaluating the Relationship Between Serum Level of Interleukin-6 and Rheumatoid Arthritis Severity and Disease Activity

2020 ◽  
Vol 16 (3) ◽  
pp. 249-255 ◽  
Author(s):  
Elham Rajaei ◽  
Karim Mowla ◽  
Qodratollah Hayati ◽  
Ali Ghorbani ◽  
Mehrdad Dargahi-Malamir ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Serum Level ◽  
Interleukin 6 ◽  
Significant Relationship ◽  
Serum Levels ◽  
P Value ◽  
Das28 Score ◽  
Cross Sectional ◽  
Das 28 ◽  
The Relationship

Aim: The aim of this study was to evaluate the relationship between Interleukin-6 (IL-6) serum level and the severity and activity of Rheumatoid Arthritis (RA). Methods: In this cross-sectional study, 120 RA patients referred to the rheumatology clinic, the patients were diagnosed by rheumatologists according to ACR / EULAR 2010 criteria. Based on DAS28 score the patients were divided into 4 groups: Remission, Mild, Moderate and Severe. Each group contained 30 patients. Serum levels of Erythrocyte Sedimentation Rate (ESR), C-Reactive Protein (CRP), anti-Cyclic Citrullinated Peptide (anti-CCP) and Rheumatoid Factor (RF) and serum levels of IL-6, were measured. The relationship between these factors was measured and compared to the relationship between IL-6 and these factors, and the activity of the disease was evaluated based on DAS-28. Results: This study showed that the serum level of IL-6 has a significant relationship with RA activity according to DAS-28 (P value <0.001). There is also a significant relationship between the ESR level, the number of painful joints, and the number of swollen joints, and the severity of the disease based on VAS. Conclusion: Generally the findings of this study indicate that serum level of IL-6 plays an important role in the severity and activity of RA disease and can be considered as a determining factor in evaluating the severity of RA in RA patients and it is a good guide for a step up or down of treatment.

scholarly journals RELATIONSHIP BETWEEN SERUM LEVELS OF IMMUNOGLOBULIN M-ANTI PHENOLIC GLYCOLIPID 1 AND IMMUNOGLOBULIN G-ANTI PHENOLIC GLYCOLIPID 1 WITH BACTERIA INDEX VALUES IN MULTIBACILLARY LEPROSY

2021 ◽  
pp. 76-79
Author(s):  
FITRIYANTI FITRIYANTI
Keyword(s):  
Immunoglobulin G ◽  
Significant Relationship ◽  
Serum Levels ◽  
Immunoglobulin M ◽  
Cross Sectional ◽  
Cross Sectional Design ◽  
Relationship Of ◽  
The Relationship ◽  
Multibacillary Leprosy ◽  
Bacterial Index

Objective: This study aims to determine the relationship of serum levels of anti-phenolic glycolipid (PGL-1), immunoglobulin M (IgM), and anti-PGL-1 immunoglobulin G (IgG) with bacterial index (BI) values in multibacillary (MB) leprosy. Methods: This study was an observational study with a cross-sectional design. A total of 30 study samples were examined for a Slit Skin Smear and examined serum levels of IgM and IgG Anti PGL-1 antibodies by ELISA method. Results: The results showed the higher the BI value, the serum levels of IgM anti-PGL-1 and IgG anti-PGL-1 increased. Conclusion: There was a significant relationship between IgM anti-PGL-1 with BI value in MB leprosy (p<0.05), but there was no significant relationship between IgG anti-PGL-1 with BI value in MB leprosy (p>0.05). IgM anti-PGL-1 has more role in increasing the value of BI in MB leprosy.

scholarly journals The Association of Thyroid Hormones With β-HCG in Patients With Hydatidiform Mole

2020 ◽  
Vol 9 (4) ◽  
pp. 140-143
Author(s):  
Hamid Reza Samimagham ◽  
Shirin Mehraban ◽  
Marzieh Nikparvar ◽  
Mohsen Arabi ◽  
Elham Boushehri ◽  
...  
Keyword(s):  
Serum Level ◽  
Thyroid Hormones ◽  
Significant Relationship ◽  
Gestational Hypertension ◽  
Hydatidiform Mole ◽  
Serum Levels ◽  
Subclinical Hyperthyroidism ◽  
Free T4 ◽  
Β Hcg ◽  
The Relationship

Background: A hydatidiform mole or molar pregnancy is the most prevalent gestational trophoblastic disease (GTD). About 55%-60% of women with trophoblastic diseases have overt hyperthyroidism at the time of diagnosis, which may have severe manifestations. This study examined the relationship between gestational hypertension and the level of thyroid hormones with beta human chorionic gonadotropin (β-HCG) in patients with a hydatidiform mole. Materials and Methods: This cross-sectional study enrolled 65 patients with a hydatidiform mole admitted to Khalij-e Fars hospital, Bandar Abbas, Iran. Patients were divided into three groups of clinical hyperthyroidism, subclinical hyperthyroidism, and healthy. The serum levels of thyroid-stimulating hormone (TSH), T4, T3, and β-HCG were checked in all subjects, and the relationship between gestational hypertension and the level of thyroid hormones with β-HCG in patients with a hydatidiform mole was examined. Results: The mean age of patients was 29.93 ± 9.04 years, and their mean gestational age was 11.09 ± 4.2 weeks. In this study, 12.3% of the patients had subclinical hyperthyroidism, 41.5% had clinical hyperthyroidism, and 46.2% were healthy. A significant relationship was found between the serum level of β-HCG and thyroid function in patients with clinical and subclinical hyperthyroidism compared to the healthy group (P= 0.001). No significant relationship was found between systolic/ diastolic blood pressure and the serum level of β-HCG. Conclusion: A significant relationship was observed between the serum levels of β-HCG and TSH, free T3, and free T4 in patients with a hydatidiform mole and thyrotoxicosis. Thus, early diagnosis of hyperthyroidism can help treat these patients more quickly.

scholarly journals Upregulation of the miRNA-155, miRNA-210, and miRNA-20b in psoriasis patients and their relation to IL-17

2020 ◽  
Vol 34 ◽  
pp. 205873842093374 ◽  
Author(s):  
Mohamed El-Komy ◽  
Iman Amin ◽  
Marwa Safwat El-Hawary ◽  
Dina Saadi ◽  
Olfat Shaker
Keyword(s):  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epigenetic Mechanisms ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Lesional Skin ◽  
Immune Mediated ◽  
Elisa Technique ◽  
Polymerase Chain ◽  
The Relationship

Psoriasis is an immune-mediated disease, with genetic background and triggering environmental factors; however, several gaps are still present in understanding the intertwined relationship between these elements. Epigenetic mechanisms, including microRNAs (miRNAs), play an important role in the pathogenesis of psoriasis. The relationship between interleukin (IL)-17, a key cytokine in psoriasis, and these epigenetic mechanisms still needs to be elucidated. This study aimed at assessing the expression of miRNA-155, miRNA-210, and miRNA-20b in skin and sera of psoriasis patients in relation to IL-17 levels. For 20 psoriasis patients and 20 matching controls, the expression of miRNA-155, miRNA-210, and miRNA-20b was assessed using real-time polymerase chain reaction (RT-PCR), whereas IL-17/IL-17A levels were measured using quantitative enzyme-linked immunosorbent assay (ELISA) technique. MiRNA-155 expression was significantly higher in lesional skin compared to controls ( P = 0.001). MiRNA-210 expression was significantly higher in both, lesional skin ( P = 0.010) and sera of patients ( P = 0.001) in comparison with controls. A statistically significant positive correlation was found between serum miRNA-210 expression and serum levels of IL-17/IL-17A ( P = 0.010, rs = 0.562). MiRNA-20b lesional and non-lesional expression was significantly higher than controls ( P < 0.001; P = 0.018). In conclusion, the expression of miRNA-155, miRNA-210, and miRNA-20b is exaggerated in psoriasis and they may be involved in disease pathogenesis. A possible relationship between miRNA-210 and IL-17 may be suggested; however, further studies are still needed to verify this relation.

scholarly journals Insight into the Metabolite Pattern of Psoriasis: Correlation among Homocysteine, Methionine, and Polyamines

Symmetry ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 606
Author(s):  
Silviya Ruseva ◽  
Inna Popova ◽  
Valentin Lozanov ◽  
Vanio Mitev
Keyword(s):  
Psoriasis Vulgaris ◽  
Serum Levels ◽  
Serum Homocysteine ◽  
Metabolite Pattern ◽  
The Relationship ◽  
Epidermal Cell Proliferation ◽  
Insight Into ◽  
Psoriasis Severity ◽  
Psoriatic Lesions ◽  
Skin Samples

Psoriasis is an incurable dermatological disorder, characterized by increased epidermal cell proliferation. Numerous studies have focused on the modulation of polyamine (PA) metabolism in psoriatic lesions, as well as the relationship between serum homocysteine (Hcy) levels and psoriasis severity. The correlation between Hcy and PA levels has not been investigated, although both of them depend on methionine (Met) loading. The aim of this study was to examine Met, Hcy, and polyamine levels in serum, saliva, lesions, and non-affected skin to investigate the eventual relationship between their levels in skin samples and to assess the correlation of each metabolite among the tested samples. This study was conducted on 24 patients with plaque-form psoriasis vulgaris. The original LC-MS/MS method was used for quantification of analytes. Hyperhomocysteinemia was observed and the serum levels of spermidine (Spd) and spermine (Spm) were also found to be elevated. A significant increase in Met (p < 0.05) and Spm (p < 0.001) concentrations in the psoriatic plaques were found, when compared to the non-affected skin. Significant correlations were established between all polyamines levels and between methionine and spermine in both types of cutaneous samples. This study illustrates the tight relationship between Met and Polyamine levels in epidermis of psoriatic patients. Our results could be helpful in psoriasis treatment, highlighting the importance of the balanced protein diet and intake of vitamins B12 and B9.

scholarly journals Elevations in Liver Transaminases in COVID-19: (How) Are They Related?

2021 ◽  
Vol 8 ◽  
Author(s):  
Henrique Pott-Junior ◽  
Natália Queiroz Prado Bittencourt ◽  
Silvana F. G. Chacha ◽  
Rafael Luís Luporini ◽  
Marcia Regina Cominetti ◽  
...  
Keyword(s):  
Disease Severity ◽  
Severe Disease ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Liver Transaminase ◽  
Cross Sectional ◽  
Inflammatory Parameters ◽  
Liver Transaminases ◽  
Altered Liver ◽  
The Relationship

Liver involvement in COVID-19 is not yet well-understood, but elevations in liver transaminases have been described to occur in 14–53% of the cases and are more frequently seen in severe disease. This cross-sectional study explored the relationship between the elevations in liver transaminases and inflammatory parameters in 209 adults with COVID-19. Demographic and clinical data, serum levels of inflammatory cytokines and liver aminotransferases were analyzed. Three groups were formed according to the liver transaminase abnormalities: (I) Normal transaminases, (II) Borderline transaminases elevation, and (III) Mild to severe transaminases elevation. Altered liver transaminases were directly related to disease severity, showing association with the NEWS2 score at admission and greater need for ICU or death. Moreover, higher levels of IL-2 and CRP were associated with borderline transaminases elevations, whereas higher levels of IL-10 and Neutrophil to Lymphocyte ratio were associated with mild to severe transaminases elevation. These results reinforce the importance of liver transaminases in patients with COVID-19 as a complementary marker for disease severity and also point to them as a parameter reflecting the continuous dynamics between viral infection and the immune response.

Predicting the Dose of Warfarin for Therapeutic Anticoagulation

1982 ◽  
Vol 47 (03) ◽  
pp. 230-231 ◽  
Author(s):  
N K Sharma ◽  
P A Routledge ◽  
M D Rawlins ◽  
D M Davies
Keyword(s):  
Therapeutic Range ◽  
Significant Relationship ◽  
Maintenance Dose ◽  
Warfarin Dose ◽  
Loading Dose ◽  
Intravenous Injections ◽  
Therapeutic Anticoagulation ◽  
Close Relationship ◽  
The Relationship ◽  
Anticoagulant Response

SummaryThe validity of a previously described technique for predicting warfarin requirements based on the anticoagulant response to a fixed loading dose was assessed prospectively in 57 patients. There was a close relationship between the predicted and initially observed daily warfarin dose required to maintain the patient within the therapeutic range for anticoagulation. The significant relationship between predicted and observed maintenance dose persisted at 4 and 12 weeks although it decreased with increasing time.The relationship between observed and predicted maintenance requirement of warfarin was not affected by the concomitant use of intermittent intravenous injections of heparin when 9 hr was allowed to elapse between the previous dose of heparin and the thrombotest estimation on which the prediction was based.It is concluded that the method is valuable in predicting an individual’s warfarin requirement, although it does not obviate the need for regular monitoring of anticoagulant control.

EL NINO RELATED CLIMATE AND DENGUE FEVER CASES IN JEDDAH, SAUDI ARABIA

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
MASROOR ALI KHAN ◽  
KHALID AL GHAMDI ◽  
JAZEM A. MEHYOUB ◽  
RAKHSHAN KHAN
Keyword(s):  
Relative Humidity ◽  
Dengue Fever ◽  
El Niño ◽  
Significant Relationship ◽  
El Nino ◽  
Climate Data ◽  
Light Traps ◽  
Mosquito Abundance ◽  
Dengue Transmission ◽  
The Relationship

The focus of this study is to find the relationship between El Nino and dengue fever cases in the study area.Mosquito density was recorded with the help of light traps and through aspirators collection. Climate data were obtained from National Meteorology and Environment centre. (Year wise El Nino and La Nina data are according to NOAA & Golden Gate Weather Services). Statistical methods were used to establish the correlation coefficient between different factors. A high significant relationship was observed between Relative Humidity and Dengue fever cases, but Aedes abundance had no significant relationship with either Relative humidity and Temperature. Our conclusion is that the El Nino does not affect the dengue transmission and Aedes mosquito abundance in this region, which is supported by earlier works.

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