scholarly journals Determination of Antioxidant 264 in the Butyl Rubber Stopper and the Compatibility with Recombinant Potent Antitumor and Antivirus Protein Injection

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Min Zhao ◽  
Xianhui Li ◽  
Di Zhang ◽  
Longshan Zhao
Keyword(s):  
High Performance ◽  
Butyl Rubber ◽  
Rubber Stopper ◽  
Extraction Solvent ◽  
Migration Study ◽  
Butyl Rubber Stopper ◽  
Brominated Butyl Rubber ◽  
And Migration

Objective. To establish a method for extraction and determination of antioxidant 264 (2,6-di-tert-butyl-4-methylphenol) in the brominated butyl rubber stopper for injection and migration study in recombinant potent antitumor and antivirus protein injection (Novaferon). Methods. Dichloromethane-ethanol was adopted as the extraction solvent during the process of reflux extraction of antioxidant 264 in the brominated butyl rubber stopper. High-performance liquid chromatography (HPLC) was used for the determination of the migration of antioxidant 264 to Novaferon. The mobile phase consisted of acetonitrile-water (80 : 20, v/v). The flow rate was 1.5 mL/min. The detection wavelength was 280 nm. Results. The linearity range was from 4.003 to 200.150 μg/mL (r2 = 0.99996), and the average recovery of antioxidant 264 was 97.8%. The applicability of the methodology was good, which can be used for the determination of antioxidant 264. The results indicated that antioxidant 264 was not detected in Novaferon after the accelerated test and three months of long-term test. Conclusion. The established validated method in this study can be used for the determination of antioxidant 264 in the rubber stopper, and the brominated butyl rubber stopper has good compatibility with Novaferon.

scholarly journals Optimization of a Method for Extraction and Determination of Residues of Selected Antimicrobials in Soil and Plant Samples Using HPLC-UV-MS/MS

2021 ◽  
Vol 18 (3) ◽  
pp. 1159
Author(s):  
Klaudia Kokoszka ◽  
Agnieszka Kobus ◽  
Sylwia Bajkacz
Keyword(s):  
Nalidixic Acid ◽  
High Performance ◽  
Solid Phase ◽  
Sewage Treatment ◽  
Animal Manure ◽  
Uv Detector ◽  
Citrate Buffer ◽  
High Biological Activity ◽  
Extraction Solvent

The residues of antimicrobials used in human and veterinary medicine are popular pollutants of anthropogenic origin. The main sources of introducing antimicrobials into the environment are sewage treatment plants and the agricultural industry. Antimicrobials in animal manure contaminate the surrounding soil as well as groundwater, and can be absorbed by plants. The presence of antimicrobials in food of plant origin may pose a threat to human health due to their high biological activity. As part of the research, a procedure was developed for the extraction and determination of ciprofloxacin, enrofloxacin, cefuroxime, nalidixic acid and metronidazole in environmental samples (soil and parsley root). An optimized solid-liquid extraction (SLE) method was used to separate antimicrobials from the solid samples and a mixture of citrate buffer (pH = 4): methanol (1:1; v/v) was used as the extraction solvent. Solid phase extraction (SPE) with OASIS® HLB cartridges was used to purify and pre-concentrate the sample. The recovery of the developed method was in the range of 55–108%. Analytes were determined by high-performance liquid chromatography coupled with an ultraviolet (UV) detector and a tandem mass spectrometer (HPLC-UV-MS/MS). The procedure was validated and applied to the determination of selected antimicrobials in soil and parsley root samples. Five types of soil and five types of parsley roots of different origins were analyzed. The presence of nalidixic acid in the parsley root samples was found in the concentration range of 0.14–0.72 ng g−1. It has been shown that antimicrobials are absorbed by the plant and can accumulate antimicrobials in its edible parts.

scholarly journals Using MoS2/Fe3O4 as Ion-Electron Transduction Layer to Manufacture All-Solid-State Ion-Selective Electrode for Determination of Serum Potassium

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 155
Author(s):  
Yan Su ◽  
Ting Liu ◽  
Caiqiao Song ◽  
Aiqiao Fan ◽  
Nan Zhu ◽  
...  
Keyword(s):  
High Performance ◽  
Accurate Determination ◽  
Water Layer ◽  
Ion Selective Electrode ◽  
Potential Response ◽  
Selective Electrode ◽  
Response Measurement ◽  
Long Term Stability

As an essential electrolyte for the human body, the potassium ion (K+) plays many physiological roles in living cells, so the rapid and accurate determination of serum K+ is of great significance. In this work, we developed a solid-contact ion-selective electrode (SC-ISE) using MoS2/Fe3O4 composites as the ion-to-electron transducer to determine serum K+. The potential response measurement of MoS2/Fe3O4/K+-ISE shows a Nernst response by a slope of 55.2 ± 0.1 mV/decade and a low detection limit of 6.3 × 10−6 M. The proposed electrode exhibits outstanding resistance to the interference of O2, CO2, light, and water layer formation. Remarkably, it also presents a high performance in potential reproducibility and long-term stability.

scholarly journals Urinary Pesticide Levels in Children and Adolescents Residing in Two Agricultural Communities in Mexico

2019 ◽  
Vol 16 (4) ◽  
pp. 562 ◽  
Author(s):  
Erick Sierra-Diaz ◽  
Alfredo Celis-de la Rosa ◽  
Felipe Lozano-Kasten ◽  
Leonardo Trasande ◽  
Alejandro Peregrina-Lucano ◽  
...  
Keyword(s):  
High Performance ◽  
Public Health Problem ◽  
Cross Sectional Study ◽  
Urine Samples ◽  
Agricultural Activities ◽  
Cross Sectional ◽  
Agricultural Communities ◽  
Health Authorities

The use of pesticides in agricultural activities has increased significantly during the last decades. Several studies have reported the health damage that results from exposure to pesticides. In Mexico, hundreds of communities depend economically on agricultural activities. The participation of minors in this type of activity and their exposure to pesticides represents a potential public health problem. A cross-sectional study was conducted, in which urine samples (first-morning urine) were taken from children under 15 years of age in both communities. A total of 281 urine samples obtained in both communities were processed for the determination of pesticides with high-performance liquid chromatography together with tandem mass spectrometry. In 100% of the samples, at least two pesticides of the 17 reported in the total samples were detected. The presence of malathion, metoxuron, and glyphosate was remarkable in more than 70% of the cases. Substantial differences were detected regarding the other compounds. It is necessary to carry out long-term studies to determine the damage to health resulting from this constant exposure and to inform the health authorities about the problem in order to implement preventive measures.

A Method for Determination of Penicillin G Residue in Waste Penicillium chrysogenum Using High Performance Liquid Chromatography

2015 ◽  
Vol 768 ◽  
pp. 15-24
Author(s):  
Pu Wang ◽  
Hui Ling Liu ◽  
Bing Wang ◽  
Xiu Wen Cheng ◽  
Qing Hua Chen ◽  
...  
Keyword(s):  
Penicillium Chrysogenum ◽  
High Performance ◽  
Extraction Process ◽  
Single Step ◽  
Penicillin G ◽  
Selective Method ◽  
Extraction Solvent ◽  
Oasis Hlb ◽  
Optimized Conditions

In this study, a rapid and selective method has been developed to determine PENG residues in waste penicillium chrysogenum by using SPE cleanup strategy followed by HPLC. Furthermore, some parameters which influenced the extraction efficiency including extraction mode, solvent and time, while washing solution and eluting solution for SPE were systematically investigated. It should be noted that the extraction process was carried out in a single step by mixing the extraction solvent acetonitrile: formic acid in aqueous solution and chrysogenum samples under ultrasound. The SPE procedure was conducted using Oasis HLB as the clean up cartridge, n-hexane as washing solution, and mixture of acetonitrile and methanol as eluting solution. Under the optimized conditions, the linear of PENG are in the range of 0.1-2000 μg/mL, with the correlation was R2>0.99. In addition, the recoveries of PENG in these samples at three fortification levels of 800-1800mg/kg were 74.98% to 113.47% are obtained, respectively. Moreover, a limits of detection (0.006 mg/kg) and quantification (0.02 mg/kg) could be achieved.

Liquid-chromatographic determination of serotonin in serum and plasma.

1978 ◽  
Vol 24 (9) ◽  
pp. 1509-1514 ◽  
Author(s):  
S Sasa ◽  
C L Blank ◽  
D C Wenke ◽  
C A Sczupak
Keyword(s):  
High Performance ◽  
Clinical Laboratory ◽  
Chromatographic Determination ◽  
Present Scheme ◽  
Long Term Storage ◽  
Significant Difference ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Using high-performance liquid chromatography with electrochemical detection, we determined serotonin in plasma from parkinsonian patients being treated with L-3,4-dihydroxyphenylalanine or N-(DL-seryl)-N'(2,3,4-trihydroxybenzyl)hydrochloride plus L-3,4-dihydroxyphenylalanine ("Sinemet") and in serum from a blood bank, from "normal" persons, and a pooled specimen from a hospital clinical laboratory. The values obtained for the two groups of Parkinson's disease patients showed no significant difference. Long-term storage on solid CO2 was xhown to be an adequate technique for preserving samples. The mean (+/-SEAM) normal value obtained for serotonin in serum was 146 +/- 46 microgram/liter (n = 23), a result in harmony with that previously obtained [Clin. Chem. 20, 812 (1974)] by fluorometry. In comparison to other methods for measurement of serotonin in serum or plasma, we believe that the present scheme offers greater selectivity, sensitivity, and precision.

Coating Quartz Arc Tube with Polycrystalline Alumina for High Intensity Discharge Lamps

2013 ◽  
Vol 718-720 ◽  
pp. 219-222
Author(s):  
Jia Qi Ju ◽  
Zhong Hang Wu ◽  
Zhen Liu Chen ◽  
Zhi Jiang He ◽  
Long He ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Tandem Mass ◽  
Phase Extraction ◽  
Polycrystalline Alumina ◽  
Extraction Solvent ◽  
Chromatography Tandem Mass Spectrometry ◽  
Dispersing Agent ◽  
Liquid Chromatography Tandem Mass

This research developed and optimized the methods for simultaneous determination of tetracycline (TCs), macrolide (MLs) and sulfonamide (SAs) antibiotics in soils using accelerated solvent extraction (ASE)- solid-phase extraction (SPE)-high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Methanol-citric acid (pH=4.7) was used as extraction solvent, diatomite (washed by EDTA) was used as dispersing agent. Firstly, soil was extracted by ASE with the parameter conditions: pressure 1500 psi, temperature 70°C, static 10 min, 1 circle, then pre-concentration by SPE and followed by HPLC-MS/MS analysis. Recovery was 86.3%~97.4% for SAs, 67.3%~87.4% for TCs and 68.4%~78.3% for MLs. RSD < 9 % and r > 0.99. Limits of detection (LOD) was 0.5~0.9 ug/kg for SAs, 0.2~1.1 ug/kg for TCs and 0.2~0.3 ug/kg for MLs. This method determined 9 kinds of antibiotics within 15 min, the determination accuracy can meet the requirements of actual analysis.

scholarly journals A Green and Efficient Method for the Preconcentration and Determination of Gallic Acid, Bergenin, Quercitrin, and Embelin from Ardisia japonica Using Nononic Surfactant Genapol X-080 as the Extraction Solvent

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Ying Chen ◽  
Kunze Du ◽  
Jin Li ◽  
Yun Bai ◽  
Mingrui An ◽  
...  
Keyword(s):  
Gallic Acid ◽  
High Performance ◽  
Solution Ph ◽  
Experimental Conditions ◽  
Micellar Extraction ◽  
Extraction Solvent ◽  
Ultrasonic Assisted ◽  
Preconcentration Factor ◽  
Interday Precision

A simple cloud point preconcentration method was developed and validated for the determination of gallic acid, bergenin, quercitrin, and embelin in Ardisia japonica by high-performance liquid chromatography (HPLC) using ultrasonic assisted micellar extraction. Nonionic surfactant Genapol X-080 was selected as the extraction solvent. The effects of various experimental conditions such as the type and concentration of surfactant and salt, temperature, and solution pH on the extraction of these components were studied to optimize the conditions of Ardisia japonica. The solution was incubated in a thermostatic water bath at 60°C for 10 min, and 35% NaH2PO4 (w/v) was added to the solution to promote the phase separation and increase the preconcentration factor. The intraday and interday precision (RSD) were both below 5.0% and the limits of detection (LOD) for the analytes were between 10 and 20 ng·mL−1. The proposed method provides a simple, efficient, and organic solvent-free method to analyze gallic acid, bergenin, quercitrin, and embelin for the quality control of Ardisia japonica.

Simultaneous Determination of Four Plant Hormones in Soil by Ultrasound-Assisted Ionic Liquid Based Dispersive Liquid-Liquid Microextraction

2014 ◽  
Vol 675-677 ◽  
pp. 181-184 ◽  
Author(s):  
Gui Qi Huang ◽  
She Ying Dong ◽  
Zhen Yang ◽  
Ting Lin Huang
Keyword(s):  
Ionic Liquid ◽  
Plant Hormones ◽  
High Performance ◽  
Extraction Solvent ◽  
Target Analytes ◽  
Relative Standard ◽  
Ultrasound Assisted ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

An ultrasound-assisted ionic liquid based dispersive liquid-liquid microextraction (UA-IL-DLLME) was developed for the determination of four plant hormones (6-benzyladenine (6-BA), kinetin (6-KT), 2, 4-dichlorophenoxy acetic acid (2, 4-D) and uniconazole (UN)) in soil, using high performance liquid chromatography (HPLC)-diode array detection (DAD). Several important parameters including the type and volume of extraction solvent, the volume of disperser solvent, ultrasound time, pH of the solution and salt effect were studied and optimized. Under optimum conditions, the limits of detections (LODs) for the target analytes were in the range of 0.002-0.01 μg g-1. And satisfactory recoveries of the target analytes in the soil samples were 79.3-96.7 %, with relative standard deviations (RSD, n=5) that ranged from 4.3 to 6.7%.

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