scholarly journals Estimation of Total Quercetin and Rutin Content in Malus domestica of Nepalese Origin by HPLC Method and Determination of Their Antioxidative Activity

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Jitendra Pandey ◽  
Tonking Bastola ◽  
Julu Tripathi ◽  
Milu Tripathi ◽  
Rabindra Kumar Rokaya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Malus Domestica ◽  
Antioxidative Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Total Carbohydrate ◽  
Dry Extract ◽  
Phytochemical Content ◽  
Hydrolysis Of

Malus domestica (Apple) is one of the most widely cultivated cash crops of Nepal. Jumla and Mustang are two major pocket areas for the production of apple. Flavonoids including quercetin and rutin are potent antioxidants present in apples. This study was designed to quantify and compare the presence of quercetin and rutin in different plant parts (peel, leaf, and bark) among various cultivars of Malus domestica from two pocket zones of Nepal. A new HPLC-UV method was developed and validated for the quantification of quercetin and rutin. Polyphenols, flavonoids, and carbohydrate contents were determined by colorimetric methods. 2,2′-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay was carried out to measure in vitro antioxidative activity. Acid hydrolysis of each extract was carried out by the standard method to measure aglycone quercetin content after hydrolysis of its glycosides. The total rutin content ranged from 3.69 ± 1.34 to 374.50 ± 2.35 mg/100g dry extract weight. Before the acidic hydrolysis, the total quercetin content ranged from 2.96 ± 0.13 to 171.05 ± 0.95 mg/100g dry extract weight whereas its amount increased highly after the hydrolysis and it ranged from 80.84 ± 19.65 to 7445.32 ± 29.25 mg/100g dry extract weight. Total polyphenol content ranged from 19.48 ± 0.23 to 123.48 ± 1.84 µg gallic acid equivalent/mg of dry extract weight. Similarly, flavonoid content ranged from 2.21 ± 0.72 µg to 755.54 ± 1.91 µg quercetin equivalent/mg of dry extract weight. Total carbohydrate content ranged from 144.15 ± 3.73 to 484.65 ± 2.63 µg glucose equivalent per 0.5 mg dry extract weight. All the extracts showed the various degrees of antioxidant activity in a dose-dependent manner. Among them, stem bark of the Jonathan Jumla showed potent antioxidant activity with IC50 value of 13.003 µg/mL. The present study provides the information about variation of the phytochemical content among the different cultivars, parts, and geographic locations. Furthermore, it revealed that bark of Malus domestica cultivars had high quercetin and rutin content with high antioxidant activity.

scholarly journals In Vitro Antioxidant Activity of Dibenz[b,f]azepine and its Analogues

2008 ◽  
Vol 5 (s2) ◽  
pp. 1123-1132 ◽  
Author(s):  
H. Vijay Kumar ◽  
C. R. Gnanendra ◽  
Nagaraja Naik ◽  
D. Channe Gowda
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Antioxidant Compounds ◽  
Dependent Manner ◽  
Concentration Dependent Manner

Dibenz[b,f]azepine and its five derivatives bearing different functional groups were synthesized by known methods. The compounds thus synthesized were evaluated for antioxidant potential through different in vitro models such as (DPPH) free radical scavenging activity,ß-carotene-linoleic acid model system, reducing power assay and phosphomolybdenum method. Under our experimental condition among the synthesized compounds dibenz[b,f]azepine (a) and 10-methoxy-5H-dibenz[b,f]azepine (d) exhibited potent antioxidant activity in concentration dependent manner in all the above four methods. Butylated hydroxyl anisole (BHA) and ascorbic acid (AA) were used as the reference antioxidant compounds. The most active compounds like dibenz[b,f]azepine and its methoxy group substituent have shown more promising antioxidant and radical scavengers compared to the standards like BHA and ascorbic acid. It is conceivable from the studies that the tricyclic amines,i.e. dibenz[b, f]azepine and some of its derivatives are effective in their antioxidant activity properties.

scholarly journals Antioxidant Activity and Acteoside Analysis of Abeliophyllum distichum

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1148 ◽  
Author(s):  
Hak-Dong Lee ◽  
Ji Hyun Kim ◽  
Qi Qi Pang ◽  
Pil-Mun Jung ◽  
Eun Ju Cho ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidative Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Nutritional Supplements ◽  
Scavenging Activity ◽  
Antioxidant Agent ◽  
Abeliophyllum Distichum ◽  
Potential Use

This study determined acteoside and its content in Abeliophyllum distichum via HPLC/UV and LC/ESI-MS to obtain insights into the potential use of this plant as an antioxidant agent. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (•OH), and O2− radical scavenging activity assays were performed to assess in vitro antioxidative activity. The DPPH, •OH, and O2− radical scavenging activities of A. distichum leaf EtOH extracts at a 250 μg/mL concentration were 88.32%, 94.48%, and 14.36%, respectively, whereas those of stem extracts at the same concentration were 88.15%, 88.99%, and 15.36%, respectively. The contents of acteoside in A. distichum leaves and stems were 162.11 and 29.68 mg/g, respectively. Acteoside was identified as the main antioxidant compound in A. distichum leaves, which resulted in DPPH, •OH, and O2− radical scavenging activities of 82.84%, 89.46%, and 30.31%, respectively, at a 25 μg/mL concentration. These results indicate that A. distichum leaves and stems containing the antioxidant acteoside can be used as natural ingredients for functional and nutritional supplements.

scholarly journals In-vitro Antioxidant Activity and Antimicrobial Activity of Hydroalcoholic Extracts of Vernonia cinerea

2019 ◽  
Vol 9 (1) ◽  
pp. 225-228
Author(s):  
Jaswinder Mehta ◽  
Peenu Mahendra Joshi ◽  
Priyanka Kushwaha ◽  
Geeta Parkhe
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Pathogenic Bacteria ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Dependent Manner ◽  
Hydroalcoholic Extract ◽  
Vernonia Cinerea

The aim of present study was to estimate the in vitro antioxidant potential and antimicrobial activity of hydroalcoholic extract of Vernonia cinerea. Antioxidant activity was assessed by using 2, 2- diphenyl-1-picryl-hydrazyl (DPPH) assay using ascorbic acid as standard antioxidant. The extract was found to scavenge effectively the free radicals. The total flavonoid contents were determined by established methods and were found to be 0.547 mg/100mg in quercitin equivalents. Antimicrobial activity was performed against 2 stains of human pathogenic bacteria by well diffusion method. Hydroalcoholic extract of Vernonia cinerea showed good antimicrobial activity against gram positive bacteria. The antioxidant activities may be attributed to the presence of significant amounts of flavonoid compounds. Results indicated that hydroalcoholic extract of Vernonia cinerea possess significant antioxidant effect in dose dependent manner, followed by the hydroalcoholic extract of Vernonia cinerea possessed good antimicrobial activity. Keywords: Antioxidant activity, Radical scavenging activity, Free radicals, Antimicrobial activity.

scholarly journals Synthesis, Spectroscopic Study and Radical Scavenging Activity of Kaempferol Derivatives: Enhanced Water Solubility and Antioxidant Activity

2019 ◽  
Vol 20 (4) ◽  
pp. 975 ◽  
Author(s):  
Sui-Ping Deng ◽  
Yi-Li Yang ◽  
Xing-Xing Cheng ◽  
Wen-Rong Li ◽  
Ji-Ye Cai
Keyword(s):  
Antioxidant Activity ◽  
Water Solubility ◽  
High Resolution Mass Spectrometry ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Thermal Methods ◽  
Vis Spectroscopy

Kaempferol (Kae) is a natural flavonoid with potent antioxidant activity, but its therapeutic use is limited by its low aqueous solubility. Here, a series of Kae derivatives were synthesized to improve Kae dissolution property in water and antioxidant activity. These compounds included sulfonated Kae (Kae-SO3), gallium (Ga) complexes with Kae (Kae-Ga) and Kae-SO3 (Kae-SO3-Ga). The compound structures were characterized by high-resolution mass spectrometry (HRMS), nuclear magnetic resonance (NMR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy and thermal methods (TG/DSC). The results showed that a sulfonic group (-SO3) was successfully tethered on the C3’ of Kae to form Kae-SO3. And in the metal complexation, 4-CO and 3-OH of the ligand participated in the coordination with Ga(III). The metal-to-ligand ratio 1:2 was suggested for both complexes. Interestingly, Kae-SO3-Ga was obviously superior to other compounds in terms of overcoming the poor water-solubility of free Kae, and the solubility of Kae-SO3-Ga was about 300-fold higher than that of Kae-Ga. Furthermore, the evaluation of antioxidant activities in vitro was carried out for Kae derivatives by using α,α-diphenyl-β-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical scavenging. The results showed that Kae-SO3-Ga was also optimal for scavenging free radicals in a dose-dependent manner. These data demonstrate that sulfonate kaempferol-gallium complex has a promising future as a potential antioxidant and as a potential therapeutic agent for further biomedical studies.

scholarly journals Evaluation of Ethnopharmacological and Antioxidant Potential ofZanthoxylum armatumDC.

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Rabia Kanwal ◽  
Muhammad Arshad ◽  
Yamin Bibi ◽  
Saira Asif ◽  
Sunbal Khalil Chaudhari
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Zanthoxylum Armatum

Zanthoxylum armatumDC. (syn.Z. alatumRoxb.) is an important medicinal plant commonly called Timur or Indian prickly ash. The ethnopharmacological study ofZ. armatumrevealed the use of different plant parts for curing various ailments including cholera, chest infection, fever, indigestion, stomach disorders, gas problems, piles, toothache, gum problems, dyspepsia, as carminative, antipyretic, aromatic, tonic, and stomachic. Keeping in view the medicinal potential of the plant, the antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power, and phosphomolybdate assay using different concentrations (7.81 μg/mL–250 μg/mL). Ascorbic acid was taken as standard. The results indicated that the free radical scavenging activity ranged from 40.12% to 78.39%, and the reductive potential ranged from 0.265 nm to 1.411 nm while the total antioxidant activity ranged from 0.124 nm to 0.183 nm. The antioxidant potential evaluated by three assays increased in a concentration dependent manner and ascorbic acid showed better antioxidant activity than leaf extract. Results obtained through different tests confirmed redox protective activities ofZanthoxylum armatum. Further in vitro and in vivo research should be performed, so this plant can be further utilized in drug development.

scholarly journals Canthium parviflorum Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Vanitha Reddy Palvai ◽  
Sowmya Mahalingu ◽  
Asna Urooj
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Temperature Stability ◽  
Dependent Manner ◽  
Oil Emulsion ◽  
Dose Dependent

Canthium parviflorum leaves were analyzed for their proximate and phytochemical composition. The leaves were extracted with methanol (ME) and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, and in vitro inhibition of Fenton’s reagent induced oxidation in oil emulsion and microsomes. In addition, the effect of high temperature (100°C, 15 and 30 min) and pH (4.5, 7, and 9) on the antioxidant activity of ME was investigated. The leaves were rich in polyphenols, flavonoids β-carotene, glutathione, α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose dependent manner. The RSA was 68%–500 μg. Reducing potency was 0.34 and FRAP was 1.377. Canthium exhibited greater inhibition of oxidation in microsomes (73%) than in the oil emulsion (21%). Heat treatment resulted in reduction of radical scavenging activity of extract from 68% to 40%. At pH 4.5 and 7 methanol extract exhibited some percent of antioxidant activity which ranged between 18 and 32%. Data indicates Canthium as a good source of antioxidants and methanol extract exhibited good antioxidant activity.

scholarly journals Formulation Evaluation and In- Vitro Antioxidant activity of Microparticles of Syzygium Cumini Plant Extract

2021 ◽  
pp. 77-85
Author(s):  
Sweta Srivastava Koka ◽  
Pravin Kumar Sharma ◽  
Vaishali Sharma ◽  
Jaya Verma ◽  
G. N. Darwhekar
Keyword(s):  
Antioxidant Activity ◽  
Plant Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Flowering Plant ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Antioxidant Supplement

Sygygium cumini, commonly known as Malabar plum, Java plum, black plum, jamun or jambolan, is an evergreen tropical tree in the flowering plant family Myrtaceae. The present study the antioxidant effects of microparticles of S. cumini plant extract were evaluated using in vitro, 2, 2-diphenylpicrylhydrazine photometric assay, total antioxidant activity and super oxide free radical scavenging activity. The microparticles showed different levels of radicals scavenging activity in a dose-dependent manner over the range of 50–150 µg/mL concentration, indicating the high antioxidative capacity of the extract. In all the experimental screening models microparticles of aqueous extract show the maximum inhibition of free radicals comparison of other experimental model at different concentrations. These findings suggest that the seed of Syginium cumini has potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.

scholarly journals Inhibition of Fe2+ Induced Lipid Peroxidation in Rats Brain by Extract of Euphorbia heterophylla in vitro

2019 ◽  
pp. 1-8
Author(s):  
Leye Jonathan Babatola ◽  
Oluwakemisola B. Oshanimi ◽  
Olanrewaju M. Oluba ◽  
Lawrence Okoror ◽  
Adewale Agboola Odutuga
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenol ◽  
Dependent Manner ◽  
Significant Difference

This study is sought to determine the antioxidant activity and protective ability of aqueous and methanol extractible phytochemicals from Euphorbia heterophylla leaves on lipid peroxidation induced in rat brain by pro-oxidant, in vitro. The extracts of the leaves were prepared, and the ability of the extracts is to inhibit 25 µM FeSO4 induced lipid peroxidation in isolated rats’ brain, were determined. Thereafter, total phenol content, reducing power (FRAP), Fe (II) chelating, and DPPH* free radical scavenging ability of the extracts was determined and considered as an index of antioxidant activity. The results revealed that the extracts inhibit malondialdehyde (MDA) production in the basal and pro-oxidant induced lipid peroxidised rats in a dose-dependent manner, [methanol 80.11%, aqueous 70.3%] with the methanol extract (MEE) significantly (P< 0.05) than that of aqueous extract (AEE). The methanol extract (0.74 ± 0.6 mg/g) had higher total phenol contents than the aqueous (0.57 ± 1.2 mg/g); likewise the methanol extract had higher reducing power (0.08 ± 0.2, 0.03 ± 0.1 mg/g), but had no significant difference in Fe (II) chelating ability (EC50= 0.34, 0.36) with DPPH* scavenging ability (EC50=0.075, 0.075). This antioxidant properties and the protective effect of this leaf could be harnessed in the management and prevention of degenerative diseases in association with oxidative stress.

Preparation of Guiqi Polysaccharide and Antioxidant Activity In Vitro

2013 ◽  
Vol 834-836 ◽  
pp. 539-542 ◽  
Author(s):  
Xiu Ying Pu ◽  
Heng Rui Wang ◽  
Wen Bo Fan ◽  
Shuang Yu
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Astragalus Membranaceus ◽  
Health Food ◽  
Total Carbohydrate ◽  
Free Radical Scavenging Assay

Polysaccharide extracts (ASP, AMP and GQP) were obtained by water extraction and ethyl alcohol deposition method from Angelica sinensis, Astragalus membranaceus and the mixture of Angelicasinensis and Astragalus membranaceus. And the total carbohydrate content of AMP, ASP and GQP was respectively determined to be 63.1%, 48.7% and 76.7% by phenol-sulfuric acid colorimetric method using glucose as the standard. The in vitro antioxidant activities of 3 polysaccharides were evaluated by free radical, superoxide radicals and DPPH free radical scavenging assay, respectively. Among 3 polysaccharides, GQP showed the highest antioxidant activity in vitro at 100-300 μg/mL. These results indicated that the GQP might be useful for developing natural safe antiaging drugs or health food.

scholarly journals Acetone Extract fromRhodomyrtus tomentosa: A Potent Natural Antioxidant

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Goodla Lavanya ◽  
Supayang Piyawan Voravuthikunchai ◽  
Nongporn Hutadilok Towatana
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Antioxidant Power ◽  
Lipid Peroxidation Inhibition

Rhodomyrtus tomentosa(Myrtaceae) has been employed in traditional Thai medicine to treat colic diarrhoea, dysentery, abscesses, haemorrhage, and gynaecopathy. In addition, it has been used to formulate skin-whitening, anti-aging and skin beautifying agents. Ethnomedical activities of this plant may be due its antioxidant property. Hence, the aim of this study was to evaluate bothin vitroandin vivoantioxidant activities ofR. tomentosaleaf extract.In vitroantioxidant activity of the extract was assessed by lipid peroxidation inhibition capacity, ferric reducing antioxidant power, and metal chelating activity.R. tomentosaextract demonstrated its free radical scavenging effects in concentration dependent manner.In vivoantioxidant activity of the extract was conducted in SwissAlbinomice. Levels of thio-barbituric acid reactive substances (TBARS), glutathione (GSH), and the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in blood, liver, and kidney were analyzed using microtitre plate photometer. Administration of CCl4caused significant increase in TBARS and decrease in GSH, SOD, CAT and GPx levels. In contrast,R. tomentosaextract (0.8 g/kg) effectively prevented these alterations and maintained the antioxidant status. The results suggest thatR. tomentosaextract can serve as a potent antioxidant.

Sign in / Sign up

Export Citation Format

Share Document