scholarly journals Beneficial Effects of Apple Vinegar on Hyperglycemia and Hyperlipidemia in Hypercaloric-Fed Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Driss Ousaaid ◽  
Hassan Laaroussi ◽  
Meryem Bakour ◽  
Asmae ElGhouizi ◽  
Abderrazak Aboulghazi ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Lipid Profile ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Radical Scavenging ◽  
Total Acidity ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Hypercaloric Diet

The main objective of this study is to determine the effects of apple vinegar on the metabolic changes caused by hypercaloric diet in Wistar rats. Apple vinegar was first analyzed to find out the total acidity, the polyphenolic and flavonoid contents, the total antioxidant capacity, and the free radical scavenging activity. In vivo study on adult male and female Wistar rats was conducted by administering a drink containing either 10% D-glucose or water (control) for five weeks. Apple vinegar is administered daily by gavage (2 mL/kg) to rats fed D-glucose for 5 weeks. The results showed that the polyphenolic content in apple vinegar was 148.02±10.16 mg GAE/100 mL, flavonoid content was 22.93±0.73 QE/100 mL, and total antioxidant capacity was 13.4±0.47 mg AAE/100 mL. Free radical IC50 apple vinegar scavenging activity (DPPH) was 0.74±0.154 μL/mL. The total acidity was (3.24±0.02 mg AAE/100 mL). The treatment during five weeks with D-glucose leads to increased plasma glucose, lipid profile, hepatic enzyme levels, urea, and creatinine. Simultaneous treatment with apple vinegar improves the parameters studied. These results clearly show that the daily consumption of vinegar can reduce the rise in blood sugar and lipid profile induced by hypercaloric diet in rats. Therefore, the use of apple vinegar would have a very beneficial effect in the prevention of metabolic disorders caused by high-caloric food.

scholarly journals In Vitro Antioxidant and Thrombolytic Activities of Bridelia Species Growing in Bangladesh

2013 ◽  
Vol 5 (2) ◽  
pp. 343-351
Author(s):  
A. Anjum ◽  
M. A. Sikder ◽  
M. R. Haque ◽  
C. M. Hasan ◽  
M. A. Rashid
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Total Antioxidant

The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tomentosa growing in Bangladesh were subjected to screening for free radical scavenging activity, total antioxidant capacity and total phenolic content. All of the methanol extracts of the these plants and their kupchan fractions showed moderate to strong free radical scavenging activity, the total antioxidant capacity and total phenolic content, of which the methanol extract of the leaf of B. verrucosa revealed highest activity having IC50 value of 6.35 ?g/ml. All the extractives of three plants were also studied for their thrombolytic potential. Among the three plants the carbon tetrachloride soluble fraction and methanol extract of leaf and aqueous soluble fraction of bark of B. tomentosa, methanol extract of bark of B. stipularis and carbon tetrachloride soluble fraction of leaf of B. verrucosa exhibited highest thrombolytic activity with clot lysis value of 41.46%, 34.85%, 37.04%, 36.45% and 33.72%, respectively. Standard streptokinase was used as positive control which exhibited 61.50% lysis of clot while the negative control water revealed 2.56% lysis of clot.Keywords: Antioxidant; Free radical scavenging; Total antioxidant capacity; Phenolic content; Thrombolytic activity; Bridelia.© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v5i2.13568        J. Sci. Res. 5 (2), 343-351 (2013)

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Frutos silvestres de Anisocapparis speciosa “pajagua naranja” composición proximal, minerales y potencial antioxidante

2021 ◽  
Vol 2 (5) ◽  
pp. 6608-6618
Author(s):  
Eva Coronel ◽  
Silvia Caballero ◽  
Gabriela Gonzalez ◽  
Miho Sunguino ◽  
Laura Mereles
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Dietary Fiber ◽  
Total Antioxidant Capacity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Indigenous Populations ◽  
Soluble Solids ◽  
Gran Chaco ◽  
Total Antioxidant

Los frutos de Anisocapparis speciosa son nativas de la Ecoregión del Gran Chaco, crecen de manera silvestre en todo este territorio, su pulpa y semillas son comestibles y muy apreciadas por las poblaciones indígenas que lo habitan, además de otros usos con propiedades medicinales atribuidas por conocimientos etnobotánicos, pero no se tienen datos sobre la composición de estos frutos regionales y sus potenciales aplicaciones. El objetivo del presente trabajo fue evaluar la composición de la pulpa y semillas de frutos en estado maduro, y el potencial antioxidante en diferentes estadíos de madurez. La composición proximal, el contenido de minerales, vitamina C y solidos solubles se realizaron según metodologías oficiales de la AOAC.  Los fenoles totales se determinaron por el método de Folin Ciocalteau y la capacidad antioxidante total por medio de la inhibición del radical ABTS. Los componentes mayoritarios en pulpa y semillas fueron carhobidratos y fibra alimentaria. La pulpa madura posee un buen contenido de vitamina C (20 mg/100g). El contenido de fenoles y capacidad antioxidante total fue mayor en la pulpa que en la semillas en estados inmaduros. Los frutos de Anisocapparis speciosa pueden representar una buena fuente de carbohidratos, fibra alimentaria, Mg y vitamina C en la dieta. La pulpa y semillas son fuentes de antioxidantes naturales. Estudios futuros sobre métodos postcosecha, almacenamiento y procesamiento serán necesarios para potenciar su uso y re-valorización en el marco de la Seguridad alimentaria en la población regional.   The native Anisocapparis speciosa fruits from Gran Chaco Ecoregion, they grow wild throughout this territory, their edible pulp and seeds are highly appreciated by the indigenous populations that inhabit it, in addition to other uses with medicinal properties attributed by ethnobotanicals knowledge, but there are no data on the composition of these regional fruits and their potential applications. The aims of the present work were to analyze the pulp and seeds composition of fruits in the ripe state, and the antioxidant potential in different stages of maturity. The proximal composition, minerals content, vitamin C and soluble solids were carried out according to official AOAC methodologies. The total phenolics compounds (TPC) by the Folin Ciocalteau method and the total antioxidant capacity by means of the inhibition of the radical ABTS were evaluated. The main components in pulp and seeds were carbohydrates and dietary fiber. The ripe pulp has a good content of vitamin C (20 mg/100g). The content of TPC and total antioxidant capacity (ABTS radical scavenging) was higher in pulp than in the seeds, in immature stages. The Anisocapparis speciosa fruits can represent a good source of carbohydrates, dietary fiber, Mg and vitamin C in the diet of regional populations. The pulp and seeds are sources of natural antioxidants. Future studies on post-harvest, storage and processing methods will be necessary to enhance its use and re-valorization within the Food Security framework.  

scholarly journals Re-evaluation of ABTS•+ Assay for Total Antioxidant Capacity of Natural Products

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Jian-Wei Dong ◽  
Le Cai ◽  
Yun Xing ◽  
Jing Yu ◽  
Zhong-Tao Ding
Keyword(s):  
Natural Products ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reaction Times ◽  
Universal Method ◽  
Stable Free Radical ◽  
Abts Assay ◽  
Total Antioxidant

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS•+) is a stable free radical frequently used for estimating the total antioxidant capacity (TAC) of natural products. The existing methods for ABTS•+ radical-scavenging activity assays are diverse in pre-diluting solvents and reaction time, which lead to errors in the TAC estimations. To develop an effective and universal method for estimating the ABTS•+ capacity accurately and reasonably, five pre-dilution solvents [methanol, ethanol, phosphate buffer (Na2HPO4-NaH2PO4, 200 mM, pH = 7.4), PBS buffer (Na2HPO4-NaH2PO4-NaCl, 200 mM, pH = 7.4), and distilled water] and different reaction times were investigated in ABTS•+ assays of five typical antioxidants. The results showed that the solvent effects were very significant. When using different pre-diluting solvents, different detection wavelengths should be selected. ABTS-+ assay could be measured within 2–10 min to obtain a rough result, which was mostly 6 min in the literature. However, full and accurate evaluation of antioxidant reactivity rather than capacity requires recording ABTS•+ loss continuously during the whole reaction period. The present study makes a recommendation for estimating the ABTS•+ capacity accurately and reasonably.

scholarly journals in vitro Screening of Sesamum indicum Seeds for Antioxidant, Phytochemical and Biological Properties

2020 ◽  
Vol 32 (9) ◽  
pp. 2262-2266
Author(s):  
SHERAZ KHAN ◽  
SANIYA HASHIM KHAN ◽  
INAM ULLAH KHAN ◽  
WALI INAM ◽  
MUHAMMAD ASIM ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Sesamum Indicum ◽  
Total Antioxidant

Herbal medicines has been the most cost-effective and valuable medical practice to cure diseases and emphasize modern health care treatment. The present research was conducted to assess the biological activities of 10 fractions obtained from methanolic extract which was derived from dried seeds of Sesamum indicum. Antioxidant activity was assessed using DPPH radical scavenging, total antioxidant capacity and total reducing power assays. Highest free radical scavenging activity (80.3 ± 1.36%), total antioxidant capacity (104.7 ± 4.04 μg AAE/mg) and ferric reducing power activity (238.76 ± 1.23 μg AAE/mg) was shown by fraction SE. Fraction SE showed the highest phenolic contents (63.72 ± 1.5 μg GAE/mg) while fraction SG sample showed highest flavonoid contents (54.62 ± 2.61 μg QE QE/mg). Antibacterial activity was performed against four selected bacterial strains including Staphylococcus aureus, Escherichia coli, Enterobacter aerogens and Bacillus subtilis. Highest inhibition was shown by fraction SD (11 ± 1.04 mm) against Staphylococcus aureus, fraction SH against Bacillus subtilis (11 ± 1.06 mm) and fraction SB against Escherichia coli. All fractions were found inactive against the selected fungal strains. While performing antileishmanial activity, fraction SC showed highest percent mortality (78%) of Leishmania tropica. In brine shrimp lethality bioassay, fraction SG showed significant LD50 value (23.48 μg/mL).

scholarly journals Antioxidants and Radical Scavenging Activities of Nigerian Soybeans (Glycine max (L.) Merr.)

2019 ◽  
pp. 1-8
Author(s):  
J. Olawuni Idowu ◽  
A. David Oyinade ◽  
S. Bamidele Funminiyi ◽  
E. Ogundepo Gbenga
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Ferrous Ion ◽  
Antioxidant Assays ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
Chelating Ability

Aim: To evaluated the antioxidant and radical scavenging ability of three different accessions (TGx-1835-10E, TGx-1987-62F and TGx 1951-3F) of soybean. Study Design: In vitro evaluation of antioxidant assays: Total phenol, Total flavonoid, Total antioxidant capacity, Ferric reducing antioxidant capacity, Cupric reducing antioxidant capacity, Ferrous Ion-chelating Ability, 2, 2-Diphenyl-2-Picryl-Hydrazyl (DPPH) and Nitric oxide (NO) radical scavenging activities. Place and Duration: Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria (August–December, 2016). Methodology: Hydroalcoholic crude extracts of TGx-1835-10E, TGx-1987-62F and TGx 1951-3F were obtained through soxhlet apparatus using 80% methanol and concentrated in a rotary evaporator at 4°C. The crude extract was then subjected to different  antioxidant assays (Total phenol, Total flavonoid, Total antioxidant capacity, Ferric reducing antioxidant capacity, Cupric reducing antioxidant capacity, Ferrous Ion-chelating Ability , DPPH and NO radical scavenging activities.) following standard procedures. Results: The results shows that TGx 1951-3F elicited the highest DPPH and NO radical scavenging activity with IC50 value of 2.61± 0.02 mg/ml and 2.58 ± 0.02 mg/ml, compared to TGx-1835-10E and TGx-1987-62F. Similarly, Ferrous Ion-chelating Ability (FIC) of TGx 1951-3F was higher with IC50 value of 1.38 ± 0.07 mg/ml, compared to TGx-1835-10E and TGx-1987-62F with an IC50 of 1.86 ± 0.16 and 2.07 ± 0.16 mg/ml. The reducing power of the three accessions expressed in terms of ascorbic acid equivalent tested using FRAP, TAC and CUPRAC assays showed that TGx 1951-3F has highest antioxidant activity follow by TGx-1835-10E and TGx-1987-62F. This same trend was also observed in antioxidant constituent present in the samples as TGx-1951-3F has higher phenolic and flavonoid content compared to TGx-1835-10E and TGx-1987-62F. Conclusion: The result of this present study revealed that Accession TGx 1951-3F elicit the highest antioxidant potential nevertheless, accessions TGx-1835-10E and TGx-1987-62F also contain significant amounts of flavonoids and phenolic compounds. Consequently, the plant seeds might be an important source of natural antioxidant, and helpful in prevention and management of various diseases associated with oxidative stress.

Effect of turmeric on glycemic status, lipid profile, hs‐CRP, and total antioxidant capacity in hyperlipidemic type 2 diabetes mellitus patients

2019 ◽  
Vol 33 (4) ◽  
pp. 1173-1181 ◽  
Author(s):  
Zohreh Adab ◽  
Shahryar Eghtesadi ◽  
Mohammad‐Reza Vafa ◽  
Iraj Heydari ◽  
Asieh Shojaii ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Antioxidant Capacity ◽  
Lipid Profile ◽  
Total Antioxidant Capacity ◽  
Glycemic Status ◽  
Total Antioxidant ◽  
Hs Crp

scholarly journals Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 533 ◽  
Author(s):  
Annalisa Chiavaroli ◽  
Koaudio Ibrahime Sinan ◽  
Gokhan Zengin ◽  
Mohamad Fawzi Mahomoodally ◽  
Nabeelah Bibi Sadeer ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Trichoderma Viride ◽  
Radical Scavenging ◽  
Dna Gyrase ◽  
Network Pharmacology ◽  
E Coli ◽  
In Silico Studies ◽  
Total Antioxidant ◽  
Reducing Potential

Mangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer-assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase and anti-cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol-14-α-demethylase and E. coli DNA gyrase, underlying the observed bio-pharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited the highest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy-trihydroxyflavone. The same compounds were also docked against lanosterol-14α-demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar–micromolar range that further support the observed anti-microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti-oxidants and enzyme inhibitors that can be exploited as future first-line pharmacophores.

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