scholarly journals An Image Recognition Framework for Oral Cancer Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Hao Zhang ◽  
Wei Li ◽  
Hanzhong Zhang

Oral squamous cell carcinoma (OSCC) is a common type of cancer of the oral cavity. Despite their great impact on mortality, sufficient screening techniques for early diagnosis of OSCC often lack accuracy and thus OSCCs are mostly diagnosed at a late stage. Early detection and accurate recognition of OSCCs would lead to an improved curative result and a reduction in recurrence rates after surgical treatment. The introduction of image recognition technology into the doctor’s diagnosis process can significantly improve cancer diagnosis, reduce individual differences, and effectively assist doctors in making the correct diagnosis of the disease. The objective of this study was to assess the precision and robustness of a deep learning-based method to automatically identify the extent of cancer on digitized oral images. We present a new method that employs different variants of convolutional neural network (CNN) for detecting cancer in oral cells. Our approach involves training the classifier on different images from the imageNet dataset and then independently validating on different cancer cells. The image is segmented using multiscale morphology methods to prepare for cell feature analysis and extraction. The method of morphological edge detection is used to more accurately extract the target, cell area, perimeter, and other multidimensional features followed by classification through CNN. For all five variants of CNN, namely, VGG16, VGG19, InceptionV3, InceptionResNetV2, and Xception, the train and value losses are less than 6%. Experimental results show that the method can be an effective tool for OSCC diagnosis.

2020 ◽  
Vol 45 (2) ◽  
pp. 738-751
Author(s):  
Koji Harada ◽  
Tarannum Ferdous ◽  
Kenji Watanabe ◽  
Yoichi Mizukami ◽  
Katsuaki Mishima

Cancers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2450
Author(s):  
Sheng-Yao Peng ◽  
Jen-Yang Tang ◽  
Ruei-Nian Li ◽  
Hurng-Wern Huang ◽  
Chang-Yi Wu ◽  
...  

Combined treatment is increasingly used to improve cancer therapy. Non-ionizing radiation ultraviolet-C (UVC) and sinularin, a coral Sinularia flexibilis-derived cembranolide, were separately reported to provide an antiproliferation function to some kinds of cancer cells. However, an antiproliferation function using the combined treatment of UVC/sinularin has not been investigated as yet. This study aimed to examine the combined antiproliferation function and explore the combination of UVC/sinularin in oral cancer cells compared to normal oral cells. Regarding cell viability, UVC/sinularin displays the synergistic and selective killing of two oral cancer cell lines, but remains non-effective for normal oral cell lines compared to treatments in terms of MTS and ATP assays. In tests using the flow cytometry, luminescence, and Western blotting methods, UVC/sinularin-treated oral cancer cells exhibited higher reactive oxygen species production, mitochondrial superoxide generation, mitochondrial membrane potential destruction, annexin V, pan-caspase, caspase 3/7, and cleaved-poly (ADP-ribose) polymerase expressions than that in normal oral cells. Accordingly, oxidative stress and apoptosis are highly induced in a combined UVC/sinularin treatment. Moreover, UVC/sinularin treatment provides higher G2/M arrest and γH2AX/8-hydroxyl-2′deoxyguanosine-detected DNA damages in oral cancer cells than in the separate treatments. A pretreatment can revert all of these changes of UVC/sinularin treatment with the antioxidant N-acetylcysteine. Taken together, UVC/sinularin acting upon oral cancer cells exhibits a synergistic and selective antiproliferation ability involving oxidative stress-dependent apoptosis and cellular DNA damage with low toxic side effects on normal oral cells.


2020 ◽  
Vol 19 (18) ◽  
pp. 2223-2230 ◽  
Author(s):  
Poonam Verma ◽  
Sanjukta Naik ◽  
Pranati Nanda ◽  
Silvi Banerjee ◽  
Satyanarayan Naik ◽  
...  

Background: Coconut oil is an edible oil obtained from fresh, mature coconut kernels. Few studies have reported the anticancer role of coconut oil. The fatty acid component of coconut oil directly targets the liver by portal circulation and as chylomicron via lymph. However, the anti-cancer activity of coconut oil against liver cancer cells and oral cancer cells is yet to be tested. The active component of coconut oil, that is responsible for the anticancer activity is not well understood. In this study, three different coconut oils, Virgin Coconut Oil (VCO), Processed Coconut Oil (PCO) and Fractionated Coconut Oil (FCO), were used. Objective: Based on previous studies, it can be hypothesized that fatty acids in coconut oil may have anticancer potential and may trigger cell death in cancer cell lines. Methods: Each cell line was treated with different concentrations of Virgin Coconut Oil (VCO), Processed Coconut Oil (PCO) and Fractionated Coconut Oil (FCO). The treated cells were assayed by MTT after 72 hr of incubation. The fatty acid composition of different coconut oils was analyzed by gas chromatography. Result: Different concentrations of coconut oils were used to treat the cells. Interestingly, the anticancer efficacy of VCO, PCO and FCO was not uniform, rather the efficacy varied from cell line to cell line. Only 20% VCO showed significant anticancer activity in HepG2 cells in comparison to 80% PCO against the KB cell line. Remarkably, 20% of PCO and 5% of FCO showed potential growth inhibition in the KB cell line as compared to 80% PCO in HepG2 cells. Moreover, there was a difference in the efficacy of VCO, PCO and FCO, which might be due to their fatty acid composition. Comparing the anticancer efficacy of VCO, PCO and FCO in this study helped to predict which class of fatty acids and which fatty acid might be associated with the anticancer activity of VCO. Conclusion: This study shows that VCO, PCO and FCO have anticancer efficacy and may be used for the treatment of cancer, especially liver and oral cancer.


Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 887
Author(s):  
Gaël Runel ◽  
Noémie Lopez-Ramirez ◽  
Julien Chlasta ◽  
Ingrid Masse

Since the crucial role of the microenvironment has been highlighted, many studies have been focused on the role of biomechanics in cancer cell growth and the invasion of the surrounding environment. Despite the search in recent years for molecular biomarkers to try to classify and stratify cancers, much effort needs to be made to take account of morphological and nanomechanical parameters that could provide supplementary information concerning tissue complexity adaptation during cancer development. The biomechanical properties of cancer cells and their surrounding extracellular matrix have actually been proposed as promising biomarkers for cancer diagnosis and prognosis. The present review first describes the main methods used to study the mechanical properties of cancer cells. Then, we address the nanomechanical description of cultured cancer cells and the crucial role of the cytoskeleton for biomechanics linked with cell morphology. Finally, we depict how studying interaction of tumor cells with their surrounding microenvironment is crucial to integrating biomechanical properties in our understanding of tumor growth and local invasion.


2010 ◽  
Vol 71 (4) ◽  
pp. 240-248 ◽  
Author(s):  
Daih-Huang Kuo ◽  
Li-Min Liu ◽  
Hsin-Wei Chen ◽  
Fu-An Chen ◽  
Chung-Ren Jan

2013 ◽  
Vol 35 (2) ◽  
pp. 178-184 ◽  
Author(s):  
Jau-Min Chien ◽  
Chiang-Ting Chou ◽  
Yi-Chau Lu ◽  
Ti Lu ◽  
Chao-Chuan Chi ◽  
...  

2013 ◽  
Vol 20 (8) ◽  
pp. 624-632 ◽  
Author(s):  
Ying-hui Chen ◽  
Lyh-Jyh Hao ◽  
Chih-peng Hung ◽  
Jung-wei Chen ◽  
Sew-fen Leu ◽  
...  

2014 ◽  
Vol 67 (1) ◽  
pp. 126-136 ◽  
Author(s):  
Zhaohu Yuan ◽  
Huili Wang ◽  
Ziyou Hu ◽  
Yanqing Huang ◽  
Fang Yao ◽  
...  

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