Strong Twirling-Rotating Manual Acupuncture with 4 r/s Is Superior to 2 r/s in Relieving Pain by Activating C-Fibers in Rat Models of CFA-Induced Pain

Background. Manual acupuncture (MA) with different stimulus frequencies may give rise to varying acupuncture effects. However, the intensity-effect relationship and the underlying mechanisms of MA remain unclear. Objective. To compare the analgesic effects of different frequencies of twirling-rotating MA on rats with complete Freund’s adjuvant- (CFA-) induced pain and explore the underlying mechanism via peripheral sensory nerves. Methods. First, 36 healthy male Wistar rats were randomly divided into 6 groups: control group, 2 r/s MA group (twirling-rotating MA with the frequency of 2 revolutions per second), 4 r/s MA group (twirling-rotating MA with the frequency of 4 revolutions per second), CFA group, CFA + 2 r/s MA group, and CFA + 4 r/s MA group. Rats in three CFA groups received an intraplantar injection of CFA to establish a pain model, while the rats in other three groups received an intraplantar injection of saline. Rats in the 2 r/s MA group and 4 r/s MA group were treated with the corresponding frequencies of twirling-rotating MA on bilateral Zusanli (ST36) and Kunlun (BL60) for 7 days. The ipsilateral nociceptive thresholds (paw withdrawal latency; PWL) were tested to evaluate the analgesic effects. Second, 9 healthy male Wistar rats were randomly divided into 3 groups: control group, 2 r/s MA group, and 4 r/s MA group. The proportion of C-fiber neurons (calcitonin gene-related peptide- (CGRP-) positive neurons) and A-fiber neurons (neurofilament 200- (NF200-) positive neurons) in the dorsal root ganglia (DRG) activated by MA were quantitatively analyzed with the morphological immunofluorescence staining method. Third, 30 healthy male Wistar rats were randomly divided into 6 groups: control group, CFA group, CFA + 2 r/s MA group, CFA + 2 r/s MA + RTX group, CFA + 4 r/s MA group, and CFA + 4 r/s MA + RTX group. Resiniferatoxin (RTX) was injected into the acupoints before acupuncture. PWL was evaluated to investigate the analgesic effect. Results. Both types of MA treatment increased the PWL of saline-injecting rats and pain model rats. Moreover, 4 r/s MA was superior to 2 r/s MA in increasing PWL. A higher quantity of excited C-fiber neurons was observed following 4 r/s MA than 2 r/s MA, while the reverse was observed in the activation of A-fiber neurons. Following the injection of RTX to inhibit the activation of C-fibers, the analgesic effect of 4 r/s MA reduced significantly but not of 2 r/s MA. Conclusion. Strong MA (4 r/s MA) has superior analgesic effects to gentle MA (2 r/s MA) on CFA model rats, which is associated with C-fiber activation.

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Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

Hang Tuah Medical journal ◽

10.30649/htmj.v15i2.66 ◽

2018 ◽

Vol 15 (2) ◽

pp. 146

Author(s):

BRILIAN DINANTI ◽

FITRI HANDAJANI

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Wistar Rats ◽

Group Method ◽

Control Group ◽

Intraplantar Injection ◽

Significant Difference ◽

Male Wistar Rats ◽

Potato Extract ◽

Purple Sweet Potato

Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (x̅= 207,50) and group of rats induced by carrageenan (x̅=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (x̅= 232,50).The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants. Keywords: Liver, Ipomoea batatas L., Malondialdehyde, Anthocyanins

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Impact of Vanadium Complexes Treatment on the Oxidative Stress Factors in Wistar Rats Plasma

Bioinorganic Chemistry and Applications ◽

10.1155/2011/206316 ◽

2011 ◽

Vol 2011 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

R. Francik ◽

M. Krośniak ◽

M. Barlik ◽

A. Kudła ◽

R. Gryboś ◽

...

Keyword(s):

Wistar Rats ◽

Reduced Glutathione ◽

The Body ◽

Stress Factors ◽

Control Group ◽

Vanadium Complexes ◽

Healthy Male ◽

Reducing Ability ◽

Male Wistar Rats ◽

Antioxidant Parameters

The aim of this study was to investigate the clinical efficacy of vanadium complexes on triglycerides (TG), total cholesterol (Chol), uric acid (UA), urea (U), and antioxidant parameters: nonenzymatic (FRAP—ferric reducing ability of plasma, and reduced glutathione—GSH) and enzymatic (glutathione peroxidase—GPx, catalase—CAT, and GPx/CAT ratio) activity in the plasma of healthy male Wistar rats. Three vanadium complexes: [VO(bpy)2]SO4⋅2H2O, [VO(4,4′Me2bpy)2]SO4⋅2H2O, and Na[VO(O2)2(bpy)]⋅8H2O are administered by gavage during 5 weeks in two different diets such as control (C) and high fatty (F) diets. Changes of biochemical and antioxidants parameters are measured in plasma. All three vanadium complexes statistically decrease the body mass growth in comparison to the control and fatty diet. In plasma GSH was statistically increased in all vanadium complexes-treated rats from control and fatty group in comparison to only control group. Calculated GPX/CAT ratio was the highest in the control group in comparison to others.

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Biochemical and Histopathological Studies of Key Tissues in Healthy Male Wistar Rats Fed on African Yam Bean Seed and Tuber Meals

Journal of Food Quality ◽

10.1155/2020/8892618 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Omena Bernard Ojuederie ◽

John Adebayo Ajiboye ◽

Olubukola Oluranti Babalola

Keyword(s):

Wistar Rats ◽

Seed Meal ◽

Control Group ◽

Human Consumption ◽

Healthy Male ◽

Ether Anesthesia ◽

African Yam Bean ◽

Group I ◽

Male Wistar Rats ◽

Histopathological Studies

Food insecurity and malnutrition are currently major issues affecting most developing countries, especially on the African continent. To mitigate this effect, focus is being given to orphan or underutilized crops with immense potentials to boost food and nutrition security in Africa, such as the African yam bean (AYB) Sphenostylis stenocarpa. The effect of AYB seed and tuber meals on the tissues of the kidney, liver, and testis of healthy male Wistar rats were investigated in this study. Four accessions of AYB were used for this study, TSs 107, TSs 140, AYB 45, and AYB 57. Thirty rats were randomly assigned into five groups (n = 6). Group I was fed on standard pelletized rat chow (control), Group II fed on 50% seed meal, Group III fed on 100% seed meal, Group IV fed on 50% tuber meal, and Group-V fed on 100% tuber meal. At the end of the treatments, the animals were sacrificed after 72 h under light ether anesthesia, and biochemical and histopathological analyses were conducted on the tissues. Phytate concentration was higher in the seeds (TSs140 (550 mg 100g−1), AYB45 (460 mg 100g−1), and AYB57 (485 mg 100g−1)) compared to the tubers (TSs140 (14.8 mg 100g−1), AYB 45 (275 mg 100g−1), and AYB57 (240 mg 100g−1)). The consumption of 100% unprocessed AYB seeds caused liver and kidney damage in rats due to increased levels of aspartate aminotransferase (5.04 ± 1.62 U L−I), alanine aminotransferase (8.46 ± 2.43 U L−I), and lipid peroxidation (0.27 ± 0.02-unit mg−1protein). AYB tubers were innocuous to Wistar rats investigated. Good processing of AYB seeds is required for safe consumption by humans and livestock. This study has shown that tubers of AYB are safe for human consumption and should be utilized in meals as it contains fewer antinutrients and had no significant effect on the tissues examined in Wistar rats.

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Shrimp diet and skin healing strength in rats

Revista de Nutrição ◽

10.1590/s1415-52732007000300004 ◽

2007 ◽

Vol 20 (3) ◽

pp. 257-263 ◽

Cited By ~ 1

Author(s):

Elizabeth Lage Borges ◽

Fernanda Kelley Silva Pereira ◽

Jacqueline Isaura Alvarez-Leite ◽

Luiz Ronaldo Alberti ◽

Mônica Alves Neves Diniz Ferreira ◽

...

Keyword(s):

Tensile Strength ◽

Wistar Rats ◽

Skin Incision ◽

Control Group ◽

Dorsal Skin ◽

Interrupted Suture ◽

Male Wistar Rats ◽

Group 2 ◽

Skin Healing ◽

Group 1

OBJECTIVE: Surgical scar tensile strength may be influenced by several factors such as drugs, hormones and diet. The purpose of the present study was to determine the influence of a shrimp-enriched diet on the tensile strength of rat scars. METHODS: Forty male Wistar rats were submitted to a 4 cm dorsal skin incision and the wounds were sutured with 5-0 nylon interrupted suture. The animals were divided into two groups: Group 1 (control) received a regular diet, and Group 2 (experimental) received a shrimp-enriched diet. The two diets contained the same amounts of proteins, lipids and carbohydrates. The rats in each group were divided into two subgroups according to the time of assessment of the scar tensile strength: subgroup A, studied on the 5th postoperative day, and subgroup B, studied on the 21st postoperative day. RESULTS: The tensile strength of the scar on the 5th postoperative day was lower in the animals that received the shrimp-enriched-diet (303.0, standard error of mean= 34.1) than in the control group (460.1, SEM = 56.7) (p<0.05). CONCLUSION: A shrimp diet reduces the tensile strength of the scar. The next step of this study will be to clarify the mechanism in which shrimp affects tensile strength.

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Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

BioScientia Medicina ◽

10.32539/bsm.v5i9.355 ◽

2021 ◽

Vol 5 (9) ◽

pp. 879-882

Author(s):

Mulyati Sri Rahayu ◽

Sri Wahyuni ◽

Yuziani

Keyword(s):

Body Weight ◽

Adult Male ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Body Weight Gain ◽

Monosodium Glutamate ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Group 2

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p <0.05). The mean of Lee index of group 2 was 70.51% lower than the control group.Conclusion: This study concluded that Lee index was not increased in MSG-treated rats than in the control group after oral MSG intervention for 21 days.

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Preliminary Study on the Effect of Methanolic Extract of Watermelon (Citrullus lanatus) Rind on Prednisolone Suppressed Immunity in Male Wistar Rats

Journal of Advances in Medical and Pharmaceutical Sciences ◽

10.9734/jamps/2021/v23i230217 ◽

2021 ◽

pp. 1-6

Author(s):

Nyejirime Young Wike ◽

Mobisson Samuel Kelechi ◽

Godspower Onyeso ◽

Okekem Amadi ◽

Elizabeth Eepho Krukru

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Methanolic Extract ◽

Citrullus Lanatus ◽

Control Group ◽

Group 4 ◽

Watermelon Rind ◽

Male Wistar Rats ◽

Group 3 ◽

Group 2

Citrullus lanatus thumb (Cucurbitaceae) commonly called watermelon is widely consumed in this part of the world as food and medicine. This study was carried out to examine the effect of methanolic extract of watermelon (Citrullus lanatus) rind on prednisolone suppressed immunity in male wistar rats. A total of 20 male wistar rats weighing 150-294g were used in 4 groups with five rats each. Group 1, the control group was given distilled water and feed, Group 2 was given 200 mg/kg body weight of methanolic extract of watermelon rind, Group 3 rats were given 2.5 mg/kg body weight of prednisolone and Group 4 rats were given 2.5 mg/kg body weight of prednisolone and 200 mg/kg body weight of methanolic extract of watermelon rind. Prednisolone and the methanolic extract of watermelon rind were administered orally for a period of 30 days. Blood samples were collected by cardio puncture from the rats for white blood cell (WBC), lymphocyte, and granulocyte and monocyte counts at the end of the experiment. The data were statistically analysed using one-way ANOVA (Analysis of variance). Data were considered significant at p<0.05. The results obtained showed that methanolic extract of watermelon rind caused a significant increasen in immune function of rats when compared with the control and immune suppressed rats.

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The effect of X-ray irradiation to the formation of polychromatic erythrocyte cell micronucleus in Wistar rats (Rattus norvegicus)

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v54.i3.p128-131 ◽

2021 ◽

Vol 54 (3) ◽

pp. 128

Author(s):

Eha Renwi Astuti ◽

Hutojo Djajakusuma ◽

Indeswati Diyatri ◽

Nastiti Faradilla Ramadhani

Keyword(s):

Treatment Group ◽

Wistar Rats ◽

Treatment Plan ◽

Statistical Significance ◽

Clinical Parameter ◽

Control Group ◽

X Ray ◽

Polychromatic Erythrocytes ◽

Male Wistar Rats ◽

Group 2

Background: Panoramic and cephalometric radiography is very important for diagnosis, treatment plan, and evaluation of orthodontic treatment results. Panoramic and cephalometric radiography are frequently performed at the same time, causing DNA damage and chromosome aberration. Purpose: This study aims to analyse the effect of X-ray exposure in panoramic and cephalometric radiography on micronuclei cell numbers. Methods: Laboratory-based analytical study with 60 healthy-male Wistar rats weighing 200–300 grams divided into 6 treatment groups (n=10). The control group: without radiographic exposure, the treatment group 2: using panoramic radiographic exposure followed by cephalometric, and the treatment group 3: using panoramic radiographic exposure and 24 hours later performed cephalometric radiographic. The unit of analysis was the polychromatic erythrocytes of mice cell, were examined 24 hours and 48 hours after irradiation had been finished. The polychromatic erythrocytes were examined using May-Gruenwald-Giemsa staining and 100x magnification under a microscope with 2000 cells per view. Data obtained were analysed using the SPSS 20 version software. The mean and standard deviations were calculated for each clinical parameter, and a one‐way ANOVA statistical test of significance was used. Statistical significance was set at p<0.05. Results: The analysis showed a significant increase (p<0.05) in the number of micronucleus in groups that used panoramic radiographic exposure followed by cephalometric. Conclusion: X-ray radiation can increase the number of micronucleus in polychromatic erythrocyte cells in rats.

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Inhibitory effect of endostatin gene therapy combined with phosphorus-32 colloid on tumour growth in Wistar rats

Bioscience Reports ◽

10.1042/bsr20160117 ◽

2016 ◽

Vol 36 (3) ◽

Cited By ~ 1

Author(s):

Huiqi Gao ◽

Jing Zhu ◽

Yong Li ◽

Peng Fu ◽

Baozhong Shen

Keyword(s):

Wistar Rats ◽

Tumour Growth ◽

Combined Therapy ◽

Inhibitory Effect ◽

Saline Control ◽

Control Group ◽

Healthy Male ◽

Saline Control Group ◽

Male Wistar Rats

Eighty healthy male Wistar rats, aged 5 weeks, weighing 100–120 g, were utilized for establishing tumour-bearing models by immediate Walker-256 cancerous ascites injection and randomly divided to four groups (n=20) treated with 0.2 ml solution containing saline, 32P-colloid (0.3 mCi), endostatin gene (20 μg), endostatin gene combined with colloid 32P. The effect of endostatin combined with a small dose of 32P-colloidal on tumour growth in vivo was evaluated and the potential mechanism underlying the combined therapy was explored. We found that 32P-colloid combined with endostatin exhibited higher inhibitory effect upon tumour growth compared with application of 32P-colloid or endostatin alone, although three therapies all significantly inhibited tumour growth compared with saline control group. The higher inhibitory effect of 32P-colloid combined with endostatin upon tumour growth might be attributed to a synergistic effect of inhibiting angiogenesis by endostatin and inducing apoptosis by 32P-colloid, as demonstrated by microvessel density (MVD) and apoptotic index (AI) measurement. Combined therapy of 32P-colloid and endostatin probably serves as a novel and efficacious therapy of tumour growth.

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Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

BioScientia Medicina ◽

10.32539/bsm.v5i4.355 ◽

2021 ◽

Vol 5 (4) ◽

pp. 851-854

Author(s):

Mulyati Sri Rahayu ◽

Sri Wahyuni ◽

Yuziani

Keyword(s):

Body Weight ◽

Adult Male ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Body Weight Gain ◽

Monosodium Glutamate ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Group 2

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Anticarcinogenic effect of probiotic fermented milk and chlorophyllin on aflatoxin-B1-induced liver carcinogenesis in rats

British Journal Of Nutrition ◽

10.1017/s0007114511003953 ◽

2011 ◽

Vol 107 (7) ◽

pp. 1006-1016 ◽

Cited By ~ 46

Author(s):

M. Kumar ◽

V. Verma ◽

R. Nagpal ◽

A. Kumar ◽

P. V. Behare ◽

...

Keyword(s):

Wistar Rats ◽

Lactobacillus Rhamnosus ◽

Thiobarbituric Acid ◽

Fermented Milk ◽

Hepatoprotective Effect ◽

Control Group ◽

Time Interval ◽

Group I ◽

Male Wistar Rats ◽

Aflatoxin B

The present investigation was carried out to evaluate the hepatoprotective effect of probiotic fermented milk (FM) containing Lactobacillus rhamnosus GG and Lactobacillus casei strain Shirota, alone as well as in combination with chlorophyllin (CHL) as an antioxidant agent in male Wistar rats administered aflatoxin-B1 (AFB1). AFB1 was injected intraperitoneally at the rate of 450 μg/kg body weight per animal twice a week for 6 weeks, maintaining an equal time interval between the two consecutive AFB1 administrations. A total of 125 male Wistar rats were randomly allocated to five groups, each group having twenty-five animals. Group I was offered FM containing L. rhamnosus GG and L. casei strain Shirota. Group II was administered AFB1 and served as the control group; group III was administered FM-AFB1, in which besides administering AFB1, FM was also offered. Group IV was offered CHL and AFB1, and group V was offered both FM and CHL along with AFB1. The rats were euthanised at the 15th and 25th week of the experiment and examined for the biochemical and hepatopathological profile. A significant reduction in thiobarbituric acid-reactive substances (TBARS) was observed in the FM–CHL–AFB1 group compared with the AFB1 control group. FM alone or in combination with CHL was found to show a significant (P < 0·05) hepatoprotective effect by lowering the levels of TBARS and by enhancing the activities of antioxidant enzymes such as glutathione peroxidase, superoxide dismutase, catalase and glutathione-S-transferase, indicating that probiotic FM alone or in combination with CHL possesses a potent protective effect against AFB1-induced hepatic damage.

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