scholarly journals Inhibitory effect of endostatin gene therapy combined with phosphorus-32 colloid on tumour growth in Wistar rats

2016 ◽  
Vol 36 (3) ◽  
Author(s):  
Huiqi Gao ◽  
Jing Zhu ◽  
Yong Li ◽  
Peng Fu ◽  
Baozhong Shen
Keyword(s):  
Wistar Rats ◽  
Tumour Growth ◽  
Combined Therapy ◽  
Inhibitory Effect ◽  
Saline Control ◽  
Control Group ◽  
Healthy Male ◽  
Saline Control Group ◽  
Male Wistar Rats

Eighty healthy male Wistar rats, aged 5 weeks, weighing 100–120 g, were utilized for establishing tumour-bearing models by immediate Walker-256 cancerous ascites injection and randomly divided to four groups (n=20) treated with 0.2 ml solution containing saline, 32P-colloid (0.3 mCi), endostatin gene (20 μg), endostatin gene combined with colloid 32P. The effect of endostatin combined with a small dose of 32P-colloidal on tumour growth in vivo was evaluated and the potential mechanism underlying the combined therapy was explored. We found that 32P-colloid combined with endostatin exhibited higher inhibitory effect upon tumour growth compared with application of 32P-colloid or endostatin alone, although three therapies all significantly inhibited tumour growth compared with saline control group. The higher inhibitory effect of 32P-colloid combined with endostatin upon tumour growth might be attributed to a synergistic effect of inhibiting angiogenesis by endostatin and inducing apoptosis by 32P-colloid, as demonstrated by microvessel density (MVD) and apoptotic index (AI) measurement. Combined therapy of 32P-colloid and endostatin probably serves as a novel and efficacious therapy of tumour growth.

scholarly journals Impact of Vanadium Complexes Treatment on the Oxidative Stress Factors in Wistar Rats Plasma

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
R. Francik ◽  
M. Krośniak ◽  
M. Barlik ◽  
A. Kudła ◽  
R. Gryboś ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Reduced Glutathione ◽  
The Body ◽  
Stress Factors ◽  
Control Group ◽  
Vanadium Complexes ◽  
Healthy Male ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Antioxidant Parameters

The aim of this study was to investigate the clinical efficacy of vanadium complexes on triglycerides (TG), total cholesterol (Chol), uric acid (UA), urea (U), and antioxidant parameters: nonenzymatic (FRAP—ferric reducing ability of plasma, and reduced glutathione—GSH) and enzymatic (glutathione peroxidase—GPx, catalase—CAT, and GPx/CAT ratio) activity in the plasma of healthy male Wistar rats. Three vanadium complexes: [VO(bpy)2]SO4⋅2H2O, [VO(4,4′Me2bpy)2]SO4⋅2H2O, and Na[VO(O2)2(bpy)]⋅8H2O are administered by gavage during 5 weeks in two different diets such as control (C) and high fatty (F) diets. Changes of biochemical and antioxidants parameters are measured in plasma. All three vanadium complexes statistically decrease the body mass growth in comparison to the control and fatty diet. In plasma GSH was statistically increased in all vanadium complexes-treated rats from control and fatty group in comparison to only control group. Calculated GPX/CAT ratio was the highest in the control group in comparison to others.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals Biochemical and Histopathological Studies of Key Tissues in Healthy Male Wistar Rats Fed on African Yam Bean Seed and Tuber Meals

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Omena Bernard Ojuederie ◽  
John Adebayo Ajiboye ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Wistar Rats ◽  
Seed Meal ◽  
Control Group ◽  
Human Consumption ◽  
Healthy Male ◽  
Ether Anesthesia ◽  
African Yam Bean ◽  
Group I ◽  
Male Wistar Rats ◽  
Histopathological Studies

Food insecurity and malnutrition are currently major issues affecting most developing countries, especially on the African continent. To mitigate this effect, focus is being given to orphan or underutilized crops with immense potentials to boost food and nutrition security in Africa, such as the African yam bean (AYB) Sphenostylis stenocarpa. The effect of AYB seed and tuber meals on the tissues of the kidney, liver, and testis of healthy male Wistar rats were investigated in this study. Four accessions of AYB were used for this study, TSs 107, TSs 140, AYB 45, and AYB 57. Thirty rats were randomly assigned into five groups (n = 6). Group I was fed on standard pelletized rat chow (control), Group II fed on 50% seed meal, Group III fed on 100% seed meal, Group IV fed on 50% tuber meal, and Group-V fed on 100% tuber meal. At the end of the treatments, the animals were sacrificed after 72 h under light ether anesthesia, and biochemical and histopathological analyses were conducted on the tissues. Phytate concentration was higher in the seeds (TSs140 (550 mg 100g−1), AYB45 (460 mg 100g−1), and AYB57 (485 mg 100g−1)) compared to the tubers (TSs140 (14.8 mg 100g−1), AYB 45 (275 mg 100g−1), and AYB57 (240 mg 100g−1)). The consumption of 100% unprocessed AYB seeds caused liver and kidney damage in rats due to increased levels of aspartate aminotransferase (5.04 ± 1.62 U L−I), alanine aminotransferase (8.46 ± 2.43 U L−I), and lipid peroxidation (0.27 ± 0.02-unit mg−1protein). AYB tubers were innocuous to Wistar rats investigated. Good processing of AYB seeds is required for safe consumption by humans and livestock. This study has shown that tubers of AYB are safe for human consumption and should be utilized in meals as it contains fewer antinutrients and had no significant effect on the tissues examined in Wistar rats.

scholarly journals Strong Twirling-Rotating Manual Acupuncture with 4 r/s Is Superior to 2 r/s in Relieving Pain by Activating C-Fibers in Rat Models of CFA-Induced Pain

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Simin Song ◽  
Yuan Xu ◽  
Jiang Liu ◽  
Yadi Jia ◽  
Xiaowei Lin ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Control Group ◽  
Healthy Male ◽  
Intraplantar Injection ◽  
Pain Model ◽  
Analgesic Effects ◽  
C Fibers ◽  
C Fiber ◽  
Male Wistar Rats ◽  
Group 2

Background. Manual acupuncture (MA) with different stimulus frequencies may give rise to varying acupuncture effects. However, the intensity-effect relationship and the underlying mechanisms of MA remain unclear. Objective. To compare the analgesic effects of different frequencies of twirling-rotating MA on rats with complete Freund’s adjuvant- (CFA-) induced pain and explore the underlying mechanism via peripheral sensory nerves. Methods. First, 36 healthy male Wistar rats were randomly divided into 6 groups: control group, 2 r/s MA group (twirling-rotating MA with the frequency of 2 revolutions per second), 4 r/s MA group (twirling-rotating MA with the frequency of 4 revolutions per second), CFA group, CFA + 2 r/s MA group, and CFA + 4 r/s MA group. Rats in three CFA groups received an intraplantar injection of CFA to establish a pain model, while the rats in other three groups received an intraplantar injection of saline. Rats in the 2 r/s MA group and 4 r/s MA group were treated with the corresponding frequencies of twirling-rotating MA on bilateral Zusanli (ST36) and Kunlun (BL60) for 7 days. The ipsilateral nociceptive thresholds (paw withdrawal latency; PWL) were tested to evaluate the analgesic effects. Second, 9 healthy male Wistar rats were randomly divided into 3 groups: control group, 2 r/s MA group, and 4 r/s MA group. The proportion of C-fiber neurons (calcitonin gene-related peptide- (CGRP-) positive neurons) and A-fiber neurons (neurofilament 200- (NF200-) positive neurons) in the dorsal root ganglia (DRG) activated by MA were quantitatively analyzed with the morphological immunofluorescence staining method. Third, 30 healthy male Wistar rats were randomly divided into 6 groups: control group, CFA group, CFA + 2 r/s MA group, CFA + 2 r/s MA + RTX group, CFA + 4 r/s MA group, and CFA  + 4 r/s MA + RTX group. Resiniferatoxin (RTX) was injected into the acupoints before acupuncture. PWL was evaluated to investigate the analgesic effect. Results. Both types of MA treatment increased the PWL of saline-injecting rats and pain model rats. Moreover, 4 r/s MA was superior to 2 r/s MA in increasing PWL. A higher quantity of excited C-fiber neurons was observed following 4 r/s MA than 2 r/s MA, while the reverse was observed in the activation of A-fiber neurons. Following the injection of RTX to inhibit the activation of C-fibers, the analgesic effect of 4 r/s MA reduced significantly but not of 2 r/s MA. Conclusion. Strong MA (4 r/s MA) has superior analgesic effects to gentle MA (2 r/s MA) on CFA model rats, which is associated with C-fiber activation.

scholarly journals Evaluation of the effect of different concentrations of Arsenicum album 6cH on intoxicated rats

2021 ◽  
Vol 8 (28) ◽  
pp. 119-127
Author(s):  
Olney Leite Fontes ◽  
Fátima Cristiane Lopes Goularte Farhat ◽  
Amarilys Toledo Cesar ◽  
Marilisa Guimarães Lara ◽  
Maria Imaculada Lima Montebelo ◽  
...  
Keyword(s):  
Atomic Absorption Spectroscopy ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups ◽  
Before And After ◽  
Male Wistar Rats

Aims: Homeopaths diverge on the concept of dose, i.e. the amount of drug that a patient must take to alter his or her state of disease. In order to stimulate reflections on this concept, this study sought to evaluate in vivo the effect of different concentrations of Arsenicum album 6cH prepared according to homeopathic pharmacotechnics. Methods: male Wistar rats were intoxicated with arsenic and then treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1%, administered orally. The amount of arsenic retained in the animals’ organism and that eliminated by urine were measured through atomic absorption spectroscopy. Samples of urine were collected before and after intoxication and during treatment. The positive control group (intoxicated animals) and the negative control group (non-intoxicated animals) received only the vehicle used in the preparation of the medicine. Results: Groups treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1% eliminated significant amounts of arsenic when compared to the control groups. The group treated with Arsenicum album 6cH eliminated significantly higher amounts of arsenic than the group treated with the diluted medicine at 1%. Conclusion: results suggest that Arsenicum album 6cH should not be diluted as not to compromise its effectiveness in the treatment of rats intoxicated with arsenic.

scholarly journals The Effectiveness of Tamarindus indica Extract in Total Osteoblast Cell of Male Wistar Rat’s Femur Bone Induced by Aluminium

2020 ◽  
Vol 6 (2) ◽  
pp. 110
Author(s):  
Indah Pratiwi ◽  
Rena Normasari ◽  
Rony Prasetyo
Keyword(s):  
Data Analysis ◽  
Wistar Rats ◽  
Control Group ◽  
Tamarindus Indica ◽  
Control Group Design ◽  
Chain Reactions ◽  
Femur Bone ◽  
Post Test ◽  
Male Wistar Rats

Aluminum is widely used in life but includes non-essential metals toxic to the human body that most accumulates in the femur. Aluminum is toxic because it increases the number of free radicals resulting in oxidative stress that causes osteoblast apoptosis. Tamarindus indica has the most potent antioxidant effect on the seed. The polyphenol content in Tamarindus indica seed are myricetin, procyanidin B2, and caffeic acid. Antioxidants in Tamarindus indica seed have mechanism of donating electrons from the -OH group in the phenolic ring to stop oxidative chain reactions and prevent the formation of hydroxyl radicals and lipid peroxidation that play a role in cell apoptosis. The purpose of this study  to determine the effect of giving Tamarindus indica extract on the total osteoblasts of femur bone in male wistar rats induced by aluminum. This research is a true experimental in vivo with randomized post-test only control group design using 25 male Wistar rats divided into five groups. Aluminum is given orally dose 300mg/kgBB and Tamarindus indica extract dose 25, 50, 100mg/kgBB for 10 weeks. The results of this study indicate the average total of osteoblasts in groups K, P1, P2, P3, P4 are 15.21 ± 1.71; 18.48 ± 3.65; 17.8 ± 7.05; 17.13 ± 1.16; 16.74 ± 5.71. Data analysis used the Kruskal Wallis test. The results of data analysis showed that there was no significant differences in all groups (p = 0.581). Based on the results of this study, Tamarindus indica extract that was given to male wistar rats induced by aluminum for 10 weeks had no effect on the total osteoblasts.

scholarly journals Influences of Anlotinib on Cytochrome P450 Enzymes in Rats Using a Cocktail Method

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Wei Sun ◽  
Zhe Wang ◽  
Ruimin Chen ◽  
Chengke Huang ◽  
Rui Sun ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Oral Administration ◽  
Inductive Effect ◽  
Saline Control ◽  
Control Group ◽  
Cytochrome P450 Enzymes ◽  
Saline Control Group ◽  
Blood Samples ◽  
Time Points

The present study aimed to investigate the effect of anlotinib (AL3818) on pharmacokinetics of cytochrome P450 (CYP) enzymes (CYP1A2, CYP2C6, CYP2D1, CYP2D2, and CYP3A1/2) by using five cocktail probe drugs in vivo. After pretreatment for 7 days with anlotinib (treatment group) or saline (control group) by oral administration, probe drugs phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam were administered to rats by oral administration. Blood samples were obtained at a series of time-points and the concentrations of five probe drugs in plasma were determined by a UHPLC-MS/MS method. The results showed that treatment with anlotinib had no significant effect on rat CYP1A2, CYP2D2, and CYP2C6. However, anlotinib had a significant inductive effect on CYP2D1 and CYP3A1/2. Therefore, caution is needed during the concomitant use of anlotinib with other drugs metabolized by CYP2D1 and CYP3A1/2 because of potential drug-anlotinib interactions.

Acute high blood glucose level attenuates histamine-stimulated acid secretion in male Wistar rats

2014 ◽  
Vol 0 (0) ◽  
Author(s):  
Taofeek O. Usman ◽  
Lawrence A. Olatunji ◽  
Abdul Rasak A. Alada
Keyword(s):  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Wistar Rats ◽  
Saline Control ◽  
Control Group ◽  
High Blood Glucose ◽  
High Blood Glucose Level ◽  
Perfusion Apparatus ◽  
Male Wistar Rats

AbstractStudies have shown that the hyperglycemic state induced by intravenous glucagon or glucose infusion may lead to inhibition of gastric acid secretion through the inhibition of gastric vagus activity. Histamine is a well-known mammalian acid secretagogue and it stimulates acid secretion through HMale Wistar rats weighing between 200 and 250 g were divided into three groups that received an intravenous infusion of normal saline (control group), glucose (hyperglycemic group), or insulin (hypoglycemic group) followed by injection of histamine to stimulate acid secretion. The oesophageal and duodenal ends of the rats’ stomach were cannulated with polythene tubing and the stomach was perfused at room temperature using a Langendoff perfusion apparatus. The effluent was collected in aliquots over 10 min per sample and its pH was measured.There was a significant (p<0.05) decrease in acid secretion following histamine injection in the hyperglycemic rats, while histamine injection led to an increase (p<0.05) in acid secretion in the hypoglycemic rats.These results show that hyperglycemic state would result in attenuated histamine-stimulated gastric acid secretion, while hypoglycemic state would lead to increased histamine-stimulated gastric acid secretion.

scholarly journals EFEK NIGELLA SATIVA OIL TERHADAP UKURAN DIAMETER ULKUS TRAUMATIKUS PADA MALE WISTAR RATS SECARA IN VIVO

2016 ◽  
Vol 3 (2) ◽  
pp. 94
Author(s):  
Linda Septiana ◽  
Ratnawati Hendari ◽  
Erwid Fatchur Rahman ◽  
Diyah Fatmasari
Keyword(s):  
Wistar Rats ◽  
Nigella Sativa ◽  
Pathological Condition ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Epithelial Tissue ◽  
Nigella Sativa Oil ◽  
Male Wistar Rats

Background: Ulcer is a pathological condition characterized by loss of epithelial tissue. Ulcer will experience healing within 2 weeks after trauma source is removed. Nigella sativa Oil has been known to heal wounds. The purpose of this study was to evaluate the effect of NSO on healing of ulcers in male wistar rats from the use of chemicals.Method: This study design was quasi-experimental methods. Ten male wistar rats were exposing the H2O2 on the mandibular anterior gingiva. divided into negatif control group and NSO group. NSO group treated twice daily for 10 days. Observaton wound size was measured on 0, 3,7, 10 days. The results were analayzed with Repeated Anova test and LSD test.Result: The observations difference diameter of traumatic ulcer negative control group and NSO on 0-10 days was 1.458 mm and 2.182 mm. The results of data analysis showed that there are significant differences (p<0,05) between negatif control group and NSO.Conclusion: NSO has an influence of the size reduction of the diameter of traumatic ulcers.

scholarly journals Effect of Notrombel on systemic hemodynamics and thrombin tolerance in the in vivo experiment

2017 ◽  
Vol 16 (4) ◽  
pp. 65-72
Author(s):  
D. L. Sonin ◽  
I. N. Dementeva ◽  
S. G. Chefu
Keyword(s):  
Wistar Rats ◽  
Gravimetric Method ◽  
Hemodynamic Effect ◽  
Systemic Hemodynamics ◽  
Control Group ◽  
Intragastric Administration ◽  
Intravenous Bolus ◽  
New Class ◽  
Male Wistar Rats

Objectives. The aim of the study was to investigate of the effect of administration of Notrombel, (one of the representatives of a new class of compounds, N,N'-substituted piperazines), on systemic hemodynamics and thrombin tolerance. Material and methods. The effect of daily intragastric administration for 14 days of Notrombel (0.01 mM/kg) and ASA (0.01 mM/kg) on the risk of spontaneous hemorrhages and thrombin tolerance (intravenous bolus thrombin administration at a dose of 50 units NIH/kg) was studied in male Wistar rats. The number of circulating platelets was determined before and 5 minutes after thrombin administration. The concentration of fibrinogen after 30 min was determined by the gravimetric method according to Rutberg R. A. Results. With the daily intragastric administration of Notrombel and ASA, spontaneous hemorrhages and increased bleeding were not observed. Blood pressure and heart rate did not differ from the control animals. Intravenous administration of thrombin caused thrombocytopenia and hypofibrinogenemia in all experimental animals. The severity of hypofibrinogenemia after thrombin administration was significantly less in rats treated with Notrombel (0.01 mM/kg) and ASA (0.01 mM/kg) than in the control group. There were no differences between Notreblel and ASA groups. Conclusions. Daily intragastric administration of Notrombel and ASA for 14 days did not cause spontaneous hemorrhages and were not associated with any adverse hemodynamic effect. Both Notrombel and ASA increased tolerance to thrombin. These data broaden the currently available knowledge related to the antithrombotic action of Notrombel.

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