scholarly journals Investigation of IL-17A Serum Levels in Patients with Nonmelanoma Skin Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Mehdi Ghahartars ◽  
Fatemeh Sedaghat ◽  
Elham Khajavi ◽  
Amir Ali Nejat ◽  
Mahyar Malekzadeh ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Cell Carcinoma ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Nonmelanoma Skin Cancer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cross Sectional ◽  
Interleukin 17A ◽  
Significant Difference

Background. Role of interleukin 17A (IL-17A) in carcinogenesis and cancer growth is controversial. Although some researches support its antitumor activity, some others suggest that it promotes the growth and development of different types of cancer including skin cancer by activation of STAT3. Although the function of the cytokines such as IL-17A has been extensively studied in various types of cancer, nonmelanoma skin cancer (NMSC) has not received much attention. Therefore, the present study was aimed to investigate the serum levels of IL-17A in NMSC patients. Methods. This cross-sectional study was performed on 60 patients with basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) as well as 57 age-sex matched healthy individuals as control group. Measurement of IL-17A serum levels in both case and control groups was performed by a commercially reliable sandwich enzyme-linked immunosorbent assay (ELISA) kit. Results. In this study, we observed that IL-17A serum levels in NMSC patients were significantly higher than the control group P < 0.001 . Also, both BCC and SCC patients had higher levels of IL-17A in their sera in comparison to the controls ( P = 0.001 and P < 0.001 , respectively). However, there was no significant difference between SCC and BCC patients regarding serum levels of IL-17A. Conclusion. According to our results, it can be concluded that IL-17A may play a role in inducing the growth and progression of NMSC and it can be used as a therapeutic target in these patients in future.

Serum levels of ARMS2, COL8A1, RAD51B, and VEGF and their correlations in Age-Related Macular Degeneration

2021 ◽  
Vol 18 ◽  
Author(s):  
Priya Battu ◽  
Kaushal Sharma ◽  
Manjari Rain ◽  
Ramandeep Singh ◽  
Akshay Anand
Keyword(s):  
Macular Degeneration ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Statistical Correlation ◽  
Age Related Macular Degeneration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cross Sectional ◽  
Age Related ◽  
Study Results

Background: Many factors including genetic and environmental are responsible for the incidence of age-related macular degeneration (AMD). However, its pathogenesis has not been clearly elucidated yet. Objective: This study aimed to estimate the Age-Related Maculopathy Susceptibility 2 (ARMS2), Collagen type VIII Alpha 1 chain (COL8A1), Rad 51 paralog(RAD51B), and Vascular Endothelial Growth Factor (VEGF) protein levels in serum of AMD and control participants and to further investigate their correlation to understand AMD pathogenesis. Methods: For this cross-sectional study, 31 healthy control and 57 AMD patients were recruited from Advanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. A blood sample was taken and serum was isolated from it. ELISA(enzyme-linked immunosorbent assay)was used for the estimation of proteins in the serum of patients. Results: ARMS2 and COL8A1 levels were significantly elevated in the AMD group than in the control group. The highest levels of ARMS2, COL8A1, and VEGF proteins were recorded for the wet AMD sub-group. The study results endorsed significant positive correlation between these following molecules; ARMS2 and COL8A1 (r=0.933, p<0.0001), ARMS2 and RAD51B (r=0.704, p<0.0001), ARMS2 and VEGF (r=0.925, p<0.0001), COL8A1 and RAD51B (r=0.736, p<0.0001), COL8A1 and VEGF (r=0.879, p<0.0001),and RAD51B and VEGF (r=0.691, p<0.0001). Conclusion: The ARMS2 and COL8A1 levels were significantly higher and RAD51B was significantly lower in the AMD group than controls. Also, a significant statistical correlation was detected between these molecules, indicating that their interaction may be involved in the pathogenesis of AMD.

scholarly journals Sero-Epidemiological Study of Hepatitis E Virus among Thalassemia as High Risk Patients: A Cross-Sectional Survey in Jahrom, Southern, Iran

2016 ◽  
Vol 8 (9) ◽  
pp. 245 ◽  
Author(s):  
Abdolreza Sotoodeh Jahromi ◽  
Abbas Ahmadi-vasmehjani ◽  
Hassan Zabetian ◽  
Hossein Hakimelahi ◽  
Alireza Yusefi ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Age Groups ◽  
Serum Levels ◽  
Hepatitis E ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Significant Difference ◽  
Ig G

<p>Hepatitis E virus (HEV) could be cause of viral hepatitis in the developing countries and cause severe epidemics. According to other studies, blood transfusion as a probable route of HEV infection has been suggested. An infection with hepatitis agents such as HEV causes active liver failure in multi-transfusion patients in particular thalassemia. The purpose of this study determines the seropositivity of anti-HEV antibodies in thalassemia individuals in Jahrom. In a cross-sectional study, sera from 110 thalassemia were collected between 2013 and 2014. Enzyme-linked immunosorbent assay (ELISA) method was performed to detection of anti-HEV antibodies. Individuals’ data were collected such as, demographic and clinical, for statistical analysis. Our results show that 10% and 1.8% of the enrolled patients were HEV Ig-G and Ig-M positive antibodies respectively. In addition, there was statiscally significant difference in age groups for prevalence of anti-HEV Ig-G (P = 0.01). Also the serum levels of liver enzymes such as ALT and AST in the HEV Ig-G and Ig-M positive samples were significantly higher than anti-HEV negative samples. But there were no significant difference between sex and splenectomy with anti-HEV positive samples. The results indicate more study are needed to assess HEV screening of blood products to these patients that those have a probably risk of exposure to HEV especially in higher years old. </p>

scholarly journals The effect of gamma radiation on plasma levels of zinc and selenium in nuclear medicine staff

2018 ◽  
Vol 21 (1) ◽  
pp. 51-56
Author(s):  
Mahdiyeh Mirnam Niha ◽  
Mehdi Salehi Barough
Keyword(s):  
Nuclear Medicine ◽  
Gamma Radiation ◽  
Graphite Furnace ◽  
Serum Zinc ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Control Group ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Mean

Background and aims: Gamma radiation is widely being used in medical-imaging centers. The aim of this study was to measure possible alterations in serum zinc and selenium levels among nuclear medicine staff. Methods: This cross-sectional study was carried out on 30 nuclear medicine staff as well as on 10 individuals as the control group. Blood samples (20 mL) were obtained from the participants and the serum specimens were isolated by centrifugation at 4000 rpm for 7 minutes. The samples were then used for measurement of zinc and selenium levels. Zinc and selenium levels were measured using atomic absorption spectroscopy (AAS) with a graphite furnace. The mean serum levels of zinc and selenium were compared using SPSS. Results: The mean level of zinc in the radiated group (70.91±14.46 μmol/L) was significantly lower than that in the control group (89.75±17.35 μmol/L) (P=0.002). A negative significant correlation was found between the duration of exposure to radiation and zinc levels (P=0.005). Furthermore, a negative significant relationship was observed between the mean radiation dose after 5 years and zinc concentration (P=0.019). Non-significant difference was found in the mean level of selenium between control (1.71±0.35 μmol/L) and radiation (2.13±1.12 μmol/L) groups. Furthermore, no significant correlation was found between selenium level and exposure time. Conclusion: Based on the results, declined level of zinc can be considered as one of the possible mechanisms caused by gamma radiation on cells which may be associated with oxidative damage. Therefore, zinc therapy can be helpful for those who work at medical radiation centers; however, it merits further studies.

scholarly journals Bone status and aortic calcifications in chondrocalcinosis patients

2020 ◽  
Vol 28 (2) ◽  
pp. 195-204
Author(s):  
Paulina Vele ◽  
Ciprian Nicolae Silaghi ◽  
Laura Otilia Damian ◽  
Siao-Pin Simon ◽  
Alexandra Craciun ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Bone Turnover Markers ◽  
Statistical Significance ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Mineral Density ◽  
Cross Sectional ◽  
Turnover Markers

AbstractAim: We aimed to examine the association between several circulating bone turnover markers [ osteocalcin (OC), osteoprotegerin (OPG), beta-CrossLaps (β-CTx)], hip and spine bone mineral density (BMD) and abdominal aortic calcification (AAC) in patients with chondrocalcinosis (CC).Methods: Thirty-six patients with CC and thirty-seven controls were consecutively enrolled in this pilot case-control, cross-sectional study. The following parameters were assessed: serum levels of OC, OPG and β-CTx by enzyme-linked immunosorbent assay (ELISA); hip and spine BMD by dual-energy X-ray absorptiometry and AAC score by lateral radiography.Results: Patients with CC had higher levels of serum bone turnover markers and AAC score than the control group: OC [6.5 (3.5-9.9) vs 4.5 (2.6-7.2) ng/ml; p=0.05], OPG [(7.7 (6.2-9.4) vs 6.5 (5.5-8.12) pmol/ml; p=0.02], β-CTx [6078 (5870-6171) vs 5851 (5465-6109) pg/ml; p=0.02] and AAC score (3.6±6.2 vs 0.5±2; p=0.006). Conversely, even if statistical significance was not reached, hip and spine BMD was lower in patients with CC. Additionally, we found a positive correlation between OPG and AAC, but also between OPG and osteoporosis in patients with CC.Conclusion: Patients with CC are characterized by higher circulating OC, OPG and β-CTx. The presence of AAC was more common in patients with CC, being only associated with serum OPG.

scholarly journals Increased Circulatory Interleukin-17A Levels in Patients with Progressive and Leukotrichial Vitiligo

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Thai Van Thanh Le ◽  
Huy Ngoc Phan ◽  
Tran Ngoc Dang ◽  
Le Duy Pham
Keyword(s):  
Lupus Erythematosus ◽  
Age Of Onset ◽  
Treatment Options ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Clinicopathological Characteristics ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Cross Sectional ◽  
Interleukin 17A

Background. Vitiligo is a chronic condition characterized by skin depigmentation. Although not life-threatening, it significantly impacts quality of life. The pathophysiology of vitiligo remains poorly understood, and treatment options are limited. Mounting evidence supports the importance of autoreactive T cells and, particularly interleukin-17A- (IL-17A-) secreting Th17 cells, in vitiligo. IL-17A targeting has been proven successful in various inflammatory dermatological conditions, including psoriasis and lupus erythematosus. Objective. We evaluated the relationship between serum levels of IL-17A and the clinicopathological characteristics of Vietnamese vitiligo patients. Methods. In this cross-sectional study, we analyzed data from 52 nonsegmental vitiligo patients and 50 age- and sex-matched healthy individuals. Serum levels of IL-17A were measured using an enzyme-linked immunosorbent assay. We evaluated the correlation between IL-17A levels and clinical characteristics including leukotrichia, disease duration, vitiligo activity, and body surface area involvement. Results. Patients with progressive vitiligo had significantly higher IL-17A levels than patients with stable vitiligo ( P  = 0.014) or healthy individuals ( P  = 0.002). In addition, serum IL-17A levels were higher in vitiligo patients with leukotrichia than in patients without it ( P  = 0.04). Furthermore, serum IL-17A levels were negatively correlated with age (r = −0.39, P  = 0.004) and age of onset (r = −0.33, P  = 0.016) in vitiligo patients. Conclusions. Higher serum levels of IL-17A in patients with progressive vitiligo and leukotrichia suggest a potential role of IL-17A in melanocyte destruction in the epidermis and the follicular matrix.

scholarly journals High Myostatin Serum Related with High Prevalence of Sarcopenia Among Elderly Population in Pedawa Village, Bali, Indonesia

2019 ◽  
Vol 11 (3) ◽  
pp. 293-8
Author(s):  
I Gusti Putu Suka Aryana ◽  
I Nyoman Astika ◽  
Raden Ayu Tuty Kuswardhani ◽  
Ida Bagus Putu Putrawan ◽  
Ni Ketut Rai Purnami ◽  
...  
Keyword(s):  
Muscle Mass ◽  
Muscle Growth ◽  
Sampling Technique ◽  
Prevalence Ratio ◽  
Serum Levels ◽  
The Elderly ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Significant Difference

BACKGROUND: Sarcopenia is defined as a decrease in muscle mass accompanied by a decrease in muscle strength and performance. Sarcopenia arises from the disruption of the complex balance between anabolic and catabolic factors. Myostatin strongly influences muscle growth inhibition. Deletion and function loss of myostatin causes hyperplasia and skeletal muscle hypertrophy.METHODS: This study was an analytical cross-sectional study. Seventy respondents aged ≥60 years in Pedawa Village, Bali, Indonesia were selected by using the stratified random sampling technique. Sarcopenia status was assessed according to Asian Working Group for Sarcopenia (AWGS) criteria, including muscle mass, grip strength, and walking speed. While the myostatin serum levels was measured by enzyme-linked immunosorbent assay (ELISA).RESULTS: The incidence of sarcopenia in the elderly was 45 people (64.3%). Based on the analysis, there was a significant difference between myostatin levels in sarcopenia subjects (47.59 ng/mL) and non-sarcopenia subjects (39.7 ng/mL). Based on the statistical calculations, it was determined that the cut-off range of myostatin levels was 48.91 ng/mL. The prevalence ratio of sarcopenia incidence based on the myostatin levels in the elderly was 3.84, while based on the combination of age risk and myostatin levels was 9.75.CONCLUSION: Based of the data, there are significant differences of myostatin level between elderly people with and without sarcopenia. The prevalence of high myostatin levels in elderly is almost 4 times higher than low myostatin levels in the elderly.KEYWORDS: myostatin, sarcopenia, elderly

scholarly journals The Difference in Interleukin-19 Serum on Degrees of Acne Vulgaris Severity

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Moerbono Mochtar ◽  
Alamanda Murasmita ◽  
M. Eko Irawanto ◽  
Indah Julianto ◽  
Harijono Kariosentono ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Acne Vulgaris ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Severe Acne ◽  
Significant Difference ◽  
The Difference ◽  
Degrees Of Severity

Introduction. Acne vulgaris is a multifactorial disease. Recent study showed that inflammation does have a central role in the formation of both inflammatory and noninflammatory lesions in acne vulgaris. There are various findings of proinflammatory cytokines related to acne vulgaris, but no previous study correlate interleukin- (IL-) 19 to acne vulgaris. This pilot study aims to look at difference in IL-19 serum concentration on degrees of severity of acne vulgaris. Methods. This is an analytical observational cross-sectional study. Sample subjects were patients with acne vulgaris who met the inclusion criteria. Enzyme-linked immunosorbent assay (ELISA) study was applied to measure IL-19 serum. Result. Analysis test found statistically significant difference between IL-19 serum concentration of group of patients with mild acne vulgaris and that of group of patients with severe acne vulgaris. Moreover, analysis revealed significant difference between IL-19 serum concentration of group of patients with moderate acne vulgaris and that of group of patients with severe acne vulgaris. Conclusions. There are differences in serum levels of IL-19 on the severity of acne vulgaris. The significant difference might show that inflammation has a core role in severity of acne vulgaris, and IL-19 might potentially be related to acne vulgaris.

scholarly journals Evaluation of the Blood Level of Adiponectin in Pregnant Adolescents

2021 ◽  
Vol 43 (06) ◽  
pp. 429-435
Author(s):  
Elaine Cristina Rocha Pádua ◽  
Silvia Daher ◽  
Isa de Pádua Cintra Sampaio ◽  
Edward Araujo Júnior ◽  
Cristina Falbo Guazzelli
Keyword(s):  
Serum Levels ◽  
Cross Sectional Study ◽  
Serum Adiponectin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sectional Study ◽  
Pregnant Adolescents ◽  
Cross Sectional ◽  
Adolescent Group ◽  
Gestational Weight ◽  
Intergroup Comparison

Abstract Objective To evaluate serum levels of adiponectin in pregnant adolescents between 30 and 36 weeks of gestation. Method: A prospective cross-sectional study enrolled 67 normal pregnant women between 30 and 36 weeks of gestation and eutrophic (body mass index [BMI]: 18.5–25 kg/m2), of which 36 were adolescents (< 20 years old) and 31 adults (≥ 20 years old). Serum adiponectin levels were determined by enzyme-linked immunosorbent assay (ELISA). The t-student or Mann-Whitney tests were used for intergroup comparison. Results Pregnant adolescents showed significantly higher serum adiponectin concentrations compared with pregnant adults (p = 0.04). No differences were observed in adiponectin levels in younger pregnant adolescents (< 16 years old) compared with older pregnant adolescents (≥ 16 years old). Adiponectin values were divided into 3 subgroups: < 3,000 ng/mL, between 3,000 and 5,000 ng/mL, and > 5,000 ng/mL. Birthweight was significantly higher in women > 5,000 ng/mL when compared with < 3,000 ng/mL in the adolescent group. No association between pregestational adiponectin levels and BMI, gestational weight gain, and gestational age was observed; however, there was a positive relation with birthweight (p = 0.0239). Conclusion Serum adiponectin values in pregnant adolescents between 30 and 36 weeks of gestation were higher compared with pregnant adults; however, no differences between younger and older pregnant adolescents were observed.

scholarly journals Anolunula in Fingernails among Patients Infected with HIV

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Pratik Gahalaut ◽  
Nitin Mishra ◽  
Sandhya Chauhan ◽  
Mir Mubashir Ali ◽  
Madhur Kant Rastogi ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Cross Sectional Study ◽  
Negative Control ◽  
Hiv Positive ◽  
Control Group ◽  
Cross Sectional ◽  
Significant Difference ◽  
Stage 1 ◽  
And Control ◽  
Hiv Negative

Lunula is the white, half-moon shaped area seen in proximal ends of some nails. Though a few studies have described the nail changes that can occur in association with HIV infection, none of these paid much attention to lunula. Aims and Objectives. To study the lunula in fingernails among HIV infected patients. Materials and Methods. An observational, cross-sectional study to record presence of lunula in 168 HIV-positive patients and compare it with age and sex matched 168 healthy HIV-negative control. Anolunula (absence of lunula) in HIV-positive patients was correlated with CD4 counts, stages of HIV infection, time since patient was diagnosed as HIV-positive, and status of antiretroviral therapy. Results. Anolunula was present in significantly more fingernails in HIV-positive patients compared to HIV-negative controls. There was a highly significant difference for total anolunula (anolunula in all fingernails) in study and control group. Incidence of total anolunula was directly proportional to the stage of HIV infection, increasing progressively as the HIV infection advances from stage 1 to stage 4. Conclusion. Absence of lunula is related to not only HIV infection per se but also the stages of HIV infection.

scholarly journals Variables associated with family breakdown in healthy and obese/ overweigh adolescents

2014 ◽  
Vol 32 (1) ◽  
pp. 70-77
Author(s):  
Carla Cristina J. N. de Almeida ◽  
Paula de Oliveira Mora ◽  
Valmir Aparecido de Oliveira ◽  
Camila Aparecida Joao ◽  
Carolina Regina Joao ◽  
...  
Keyword(s):  
Public School ◽  
Cross Sectional Study ◽  
Control Group ◽  
Sectional Study ◽  
Cross Sectional ◽  
Obese Adolescents ◽  
Significant Difference ◽  
Family Breakdown

Objective: To evaluate the presence of family breakdown factors among eutrophic and overweight/obese adolescents. Methods: Cross-sectional study of 242 students aged between 14 and 19 years old, from a public school. Each student was weighed, measured and answered a questionnaire with closed questions addressing the presence of family breakdown factors. The adolescents were divided in two groups: euthophic and overweight/obese. The answers of both groups were compared by Fisher's exact and Mann-Whitney tests. Results: There was no statistically significant difference in the prevalence of the studied factors between the two groups. Comparing the number of positive answers (presence of family breakdown factors) and negative ones (absence of family breakdown factors), no difference was observed between the groups. Conclusions: The inclusion of a control group showed that factors of family breakdown, usually identified as associated with obesity in adolescents, may also be present in eutrophic adolescents.

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