scholarly journals Sero-Epidemiological Study of Hepatitis E Virus among Thalassemia as High Risk Patients: A Cross-Sectional Survey in Jahrom, Southern, Iran

2016 ◽  
Vol 8 (9) ◽  
pp. 245 ◽  
Author(s):  
Abdolreza Sotoodeh Jahromi ◽  
Abbas Ahmadi-vasmehjani ◽  
Hassan Zabetian ◽  
Hossein Hakimelahi ◽  
Alireza Yusefi ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Age Groups ◽  
Serum Levels ◽  
Hepatitis E ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Significant Difference ◽  
Ig G

<p>Hepatitis E virus (HEV) could be cause of viral hepatitis in the developing countries and cause severe epidemics. According to other studies, blood transfusion as a probable route of HEV infection has been suggested. An infection with hepatitis agents such as HEV causes active liver failure in multi-transfusion patients in particular thalassemia. The purpose of this study determines the seropositivity of anti-HEV antibodies in thalassemia individuals in Jahrom. In a cross-sectional study, sera from 110 thalassemia were collected between 2013 and 2014. Enzyme-linked immunosorbent assay (ELISA) method was performed to detection of anti-HEV antibodies. Individuals’ data were collected such as, demographic and clinical, for statistical analysis. Our results show that 10% and 1.8% of the enrolled patients were HEV Ig-G and Ig-M positive antibodies respectively. In addition, there was statiscally significant difference in age groups for prevalence of anti-HEV Ig-G (P = 0.01). Also the serum levels of liver enzymes such as ALT and AST in the HEV Ig-G and Ig-M positive samples were significantly higher than anti-HEV negative samples. But there were no significant difference between sex and splenectomy with anti-HEV positive samples. The results indicate more study are needed to assess HEV screening of blood products to these patients that those have a probably risk of exposure to HEV especially in higher years old. </p>

Serological and Molecular Investigation of Swine Hepatitis E Virus in Pigs Raised in Southern Italy

2015 ◽  
Vol 78 (11) ◽  
pp. 2099-2102 ◽  
Author(s):  
NICOLA COSTANZO ◽  
ELEONORA SARNO ◽  
VINCENZO PERETTI ◽  
LUCIA CIAMBRONE ◽  
FRANCESCO CASALINUOVO ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Southern Italy ◽  
Age Groups ◽  
Hepatitis E ◽  
Developed Countries ◽  
Oral Route ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Genomes ◽  
Reverse Transcription Pcr ◽  
Significant Difference

Hepatitis E virus (HEV) infection is a common acute hepatitis transmitted by the fecal-oral route. In developed countries, the virus has a zoonotic potential, and domestic pigs and wild boars are considered main reservoirs. To assess the prevalence of HEV-positive animals in the Calabria region (southern Italy) on a serological and molecular level, a total of 216 autochthonous healthy pigs (Apulo-Calabrese breed) were sampled. Both sera and feces were collected. Pigs were grouped based on age: 117 pigs &lt;6 months and 99 pigs &gt;6 months. By using a commercial enzyme-linked immunosorbent assay system, a total of 173 (80%) of the 216 pigs tested seropositive. In all sampled farms (n = 8), pigs with antibodies (immunoglobulin G) against HEV were detected at a level higher than 60%, with a significant difference among age groups (P &lt; 0.0001). Moreover, 16 fattening pigs were found to be nested reverse transcription PCR positive and thus to shed viral genomes in their feces. These positive findings resulted in a prevalence of 48.4% on the farm level (16 of 35 pigs) and an overall prevalence of 7.4% at the animal level (16 of 216 pigs). Based on the present study, HEV seems to circulate among the autochthonous domestic pig population of southern Italy with a low sharing rate. Further studies exploring the origin of infection are needed to minimize the risk of human exposure and to reduce consequences for public health.

scholarly journals Degree Of Proteinuria In Post-Icteric State Of Hepatitis E Virus (Hev) Infected Patients

2012 ◽  
Vol 7 (2) ◽  
pp. 37-39
Author(s):  
W Nargis ◽  
BU Ahamed ◽  
S Zabeen ◽  
F Alam ◽  
MA Wahab ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Cross Sectional Study ◽  
Urinary Protein ◽  
Age Group ◽  
Cross Sectional ◽  
Group I ◽  
Significant Difference ◽  
The Mean ◽  
Group Ii

Introduction: Nephropathies, as one of the multiple extrahepatic features of Hepatitis E virus (HEV) infection, can occur in clinically improved HEV hepatitis patients which in majority of the cases remain clinically silent for a long period. By the time these are reported, patients have already developed renal insufficiency which may even lead to renal replacement therapy. Proteinuria, a simple test in practice, can be a useful tool for early detection of the underlying renal impairment. Objective: The aim of this study was to detect the presence of proteinuria and to evaluate the degree of proteinuria in HEV- hepatitis patients during post-icteric state. Materials and Methods: This cross sectional study was conducted on 50 diagnosed patients of clinically improved HEV-hepatitis at the Department of Biochemistry, Bangabandhu Sheikh Mujib Medical University (BSMMU) with active cooperation of the Department of Hepatology, BSMMU, between July 2006 and June 2007. Cases were chosen carefully, to exclude the acute state of illness and the patients of HEV were selected during their third or fourth follow-up, in their post-icteric phase. The study subjects were grouped according to equal age ranges in group-I (18-26 year) and group-II (27-35 year). Depending on the level of spot urinary protein (mg/dl) the subjects were also categorized as having trace, mild and moderate proteinuria. Results: The mean age of HEV infected subjects was 24.72±2.59 years. The mean spot urinary protein in age group-II patients was raised compared to age group-I and the difference was highly significant (p<0.001). Moreover, there was no significant difference (p>0.05) of spot urinary protein of male and female. Majority of HEV patients (42%) presented with mild proteinuria and mostly (53.3%) was of age group-I. Conclusion: Spot urinary protein concentration should be checked in every HEV- hepatitis patient to detect the presence and level of proteinuria. DOI: http://dx.doi.org/10.3329/jafmc.v7i2.10395 JAFMC 2011; 7(2): 37-39

scholarly journals Seroprevalence of Hepatitis E Virus Infection Among People Living With HIV in the Central African Republic

2018 ◽  
Vol 5 (12) ◽  
Author(s):  
Ornella Anne Demi Sibiro ◽  
Alexandre Manirakiza ◽  
Narcisse Patrice Komas
Keyword(s):  
Hepatitis E Virus ◽  
Central African Republic ◽  
Hepatitis E ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
People Living With Hiv ◽  
Cross Sectional ◽  
Molecular Tests ◽  
Cd4 Lymphocyte ◽  
Living With Hiv

Abstract Background Hepatitis E virus (HEV) is a waterborne virus that causes acute hepatitis in immunocompromised patients and those who are immunocompetent. Few cases of chronic HEV have been described in industrialized countries. The Central African Republic is 1 of the few countries in the world that is endemic for both HIV and HEV. The prevalence of HIV infection is estimated to be 4.9% among adults aged 15–49 years, and hepatitis E is epidemo-endemic. The aim of this study was to characterize the epidemiology of HEV infection in people living with HIV (PLHIV) in Bangui. Methods A cross-sectional study was conducted between April and September 2015 based on answers to a questionnaire, and blood samples were collected for determination of immunological markers by enzyme-linked immunosorbent assay and molecular tests. Results Of the 200 PLHIV included, 15 (7.5%) had the IgM that characterizes acute HEV infection; 8.9% were women, and 2.2% were men. The overall seroprevalence of IgG was 68% (48% women and 70.4% men), indicating that men are statistically significantly more exposed to HEV than women. HEV infection had no effect on transaminase or T-CD4+ lymphocyte cell levels. The origin of infection could not be identified. Conclusions The seroprevalence of HEV is very high among PLHIV and in the general population of Bangui. This must be taken into account in studies of the risk factors of PLHIV infected with HEV.

scholarly journals Investigation of IL-17A Serum Levels in Patients with Nonmelanoma Skin Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Mehdi Ghahartars ◽  
Fatemeh Sedaghat ◽  
Elham Khajavi ◽  
Amir Ali Nejat ◽  
Mahyar Malekzadeh ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Cell Carcinoma ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Nonmelanoma Skin Cancer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cross Sectional ◽  
Interleukin 17A ◽  
Significant Difference

Background. Role of interleukin 17A (IL-17A) in carcinogenesis and cancer growth is controversial. Although some researches support its antitumor activity, some others suggest that it promotes the growth and development of different types of cancer including skin cancer by activation of STAT3. Although the function of the cytokines such as IL-17A has been extensively studied in various types of cancer, nonmelanoma skin cancer (NMSC) has not received much attention. Therefore, the present study was aimed to investigate the serum levels of IL-17A in NMSC patients. Methods. This cross-sectional study was performed on 60 patients with basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) as well as 57 age-sex matched healthy individuals as control group. Measurement of IL-17A serum levels in both case and control groups was performed by a commercially reliable sandwich enzyme-linked immunosorbent assay (ELISA) kit. Results. In this study, we observed that IL-17A serum levels in NMSC patients were significantly higher than the control group P < 0.001 . Also, both BCC and SCC patients had higher levels of IL-17A in their sera in comparison to the controls ( P = 0.001 and P < 0.001 , respectively). However, there was no significant difference between SCC and BCC patients regarding serum levels of IL-17A. Conclusion. According to our results, it can be concluded that IL-17A may play a role in inducing the growth and progression of NMSC and it can be used as a therapeutic target in these patients in future.

scholarly journals High Myostatin Serum Related with High Prevalence of Sarcopenia Among Elderly Population in Pedawa Village, Bali, Indonesia

2019 ◽  
Vol 11 (3) ◽  
pp. 293-8
Author(s):  
I Gusti Putu Suka Aryana ◽  
I Nyoman Astika ◽  
Raden Ayu Tuty Kuswardhani ◽  
Ida Bagus Putu Putrawan ◽  
Ni Ketut Rai Purnami ◽  
...  
Keyword(s):  
Muscle Mass ◽  
Muscle Growth ◽  
Sampling Technique ◽  
Prevalence Ratio ◽  
Serum Levels ◽  
The Elderly ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Significant Difference

BACKGROUND: Sarcopenia is defined as a decrease in muscle mass accompanied by a decrease in muscle strength and performance. Sarcopenia arises from the disruption of the complex balance between anabolic and catabolic factors. Myostatin strongly influences muscle growth inhibition. Deletion and function loss of myostatin causes hyperplasia and skeletal muscle hypertrophy.METHODS: This study was an analytical cross-sectional study. Seventy respondents aged ≥60 years in Pedawa Village, Bali, Indonesia were selected by using the stratified random sampling technique. Sarcopenia status was assessed according to Asian Working Group for Sarcopenia (AWGS) criteria, including muscle mass, grip strength, and walking speed. While the myostatin serum levels was measured by enzyme-linked immunosorbent assay (ELISA).RESULTS: The incidence of sarcopenia in the elderly was 45 people (64.3%). Based on the analysis, there was a significant difference between myostatin levels in sarcopenia subjects (47.59 ng/mL) and non-sarcopenia subjects (39.7 ng/mL). Based on the statistical calculations, it was determined that the cut-off range of myostatin levels was 48.91 ng/mL. The prevalence ratio of sarcopenia incidence based on the myostatin levels in the elderly was 3.84, while based on the combination of age risk and myostatin levels was 9.75.CONCLUSION: Based of the data, there are significant differences of myostatin level between elderly people with and without sarcopenia. The prevalence of high myostatin levels in elderly is almost 4 times higher than low myostatin levels in the elderly.KEYWORDS: myostatin, sarcopenia, elderly

scholarly journals The Difference in Interleukin-19 Serum on Degrees of Acne Vulgaris Severity

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Moerbono Mochtar ◽  
Alamanda Murasmita ◽  
M. Eko Irawanto ◽  
Indah Julianto ◽  
Harijono Kariosentono ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Acne Vulgaris ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Severe Acne ◽  
Significant Difference ◽  
The Difference ◽  
Degrees Of Severity

Introduction. Acne vulgaris is a multifactorial disease. Recent study showed that inflammation does have a central role in the formation of both inflammatory and noninflammatory lesions in acne vulgaris. There are various findings of proinflammatory cytokines related to acne vulgaris, but no previous study correlate interleukin- (IL-) 19 to acne vulgaris. This pilot study aims to look at difference in IL-19 serum concentration on degrees of severity of acne vulgaris. Methods. This is an analytical observational cross-sectional study. Sample subjects were patients with acne vulgaris who met the inclusion criteria. Enzyme-linked immunosorbent assay (ELISA) study was applied to measure IL-19 serum. Result. Analysis test found statistically significant difference between IL-19 serum concentration of group of patients with mild acne vulgaris and that of group of patients with severe acne vulgaris. Moreover, analysis revealed significant difference between IL-19 serum concentration of group of patients with moderate acne vulgaris and that of group of patients with severe acne vulgaris. Conclusions. There are differences in serum levels of IL-19 on the severity of acne vulgaris. The significant difference might show that inflammation has a core role in severity of acne vulgaris, and IL-19 might potentially be related to acne vulgaris.

scholarly journals Seroprevalence of hepatitis E virus in risk populations and blood donors in a referral hospital in the south of Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marisa Boff Costa ◽  
Michele Soares Gomes Gouvêa ◽  
Samira Chuffi ◽  
Gustavo Hirata Dellavia ◽  
Felipe Ornel ◽  
...  
Keyword(s):  
Low Income ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Total Sample ◽  
Hepatitis E ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Confirmatory Test ◽  
Cross Sectional ◽  
Populations At Risk

AbstractThe prevalence of anti-hepatitis E virus (HEV) antibodies has a high heterogeneity worldwide. South American data are still scarce. The aim of this study was to evaluate the prevalence of HEV in populations at risk in comparison to blood donors (BD). A cross-sectional study was carried out in adults of different risk populations including crack users (CK), residents in a low income area (LIA), cirrhotic (CIR) and liver transplant patients (LT) compared with BD. The WANTAI HEV ELISA test was used and real-time PCR (in-house for screening and ALTONA as confirmatory test) for HEV RNA screening. A total of 400 participants were included. Anti-HEV IgG was positive in 19.5% of the total sample, reaching the highest rate in the CIR group, 22.5%, followed by CK, LT, and LIA (20%, 18.7%, and 17.5%, respectively). The prevalence found in BD individuals was of 18.7% (p = NS). Anti-HEV IgM was positive in only 1.5% of the sample (6/400). No blood or stools samples were positive for HEV RNA. The seroprevalence reported is among the highest rates ever found in Brazil. Considering the intense diagnostic investigation, data show that HEV circulation is more common that might be expected in our country.

scholarly journals Evaluation of the Blood Level of Adiponectin in Pregnant Adolescents

2021 ◽  
Vol 43 (06) ◽  
pp. 429-435
Author(s):  
Elaine Cristina Rocha Pádua ◽  
Silvia Daher ◽  
Isa de Pádua Cintra Sampaio ◽  
Edward Araujo Júnior ◽  
Cristina Falbo Guazzelli
Keyword(s):  
Serum Levels ◽  
Cross Sectional Study ◽  
Serum Adiponectin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sectional Study ◽  
Pregnant Adolescents ◽  
Cross Sectional ◽  
Adolescent Group ◽  
Gestational Weight ◽  
Intergroup Comparison

Abstract Objective To evaluate serum levels of adiponectin in pregnant adolescents between 30 and 36 weeks of gestation. Method: A prospective cross-sectional study enrolled 67 normal pregnant women between 30 and 36 weeks of gestation and eutrophic (body mass index [BMI]: 18.5–25 kg/m2), of which 36 were adolescents (< 20 years old) and 31 adults (≥ 20 years old). Serum adiponectin levels were determined by enzyme-linked immunosorbent assay (ELISA). The t-student or Mann-Whitney tests were used for intergroup comparison. Results Pregnant adolescents showed significantly higher serum adiponectin concentrations compared with pregnant adults (p = 0.04). No differences were observed in adiponectin levels in younger pregnant adolescents (< 16 years old) compared with older pregnant adolescents (≥ 16 years old). Adiponectin values were divided into 3 subgroups: < 3,000 ng/mL, between 3,000 and 5,000 ng/mL, and > 5,000 ng/mL. Birthweight was significantly higher in women > 5,000 ng/mL when compared with < 3,000 ng/mL in the adolescent group. No association between pregestational adiponectin levels and BMI, gestational weight gain, and gestational age was observed; however, there was a positive relation with birthweight (p = 0.0239). Conclusion Serum adiponectin values in pregnant adolescents between 30 and 36 weeks of gestation were higher compared with pregnant adults; however, no differences between younger and older pregnant adolescents were observed.

The influence of varying maxillary central incisor vertical dimension on perceived smile aesthetics

2019 ◽  
Vol 46 (2) ◽  
pp. 137-142
Author(s):  
Elis Janaina Lira dos Santos ◽  
Alana Moura Xavier Dantas ◽  
Raíssa Marçal Vilela ◽  
Karina Jerônimo Rodrigues Santiago de Lima ◽  
Rejane Targino Soares Beltrão
Keyword(s):  
Age Groups ◽  
Educational Background ◽  
Cross Sectional Study ◽  
Central Incisor ◽  
Chi Square ◽  
Cross Sectional ◽  
Exact Test ◽  
Maxillary Central Incisor ◽  
Significant Difference ◽  
Chi Square Test

Objective: To evaluate patient perception of the smile focused on analysis of the upper incisors. Design: Cross-sectional study. Setting: Department of Clinical and Social Dentistry at Federal University of Paraíba, João Pessoa, Brazil. Participants: The sample consisted of 135 individuals (73 women and 62 men). Methods: Images with different characteristics associated with the incisal edges were shown at random and analysed using a Likert scale. Data were analysed using the Chi-square test or Fisher’s exact test with a statistically significant difference ( P < 0.05). Results: Smile I (difference of 1.5 mm in relation to the central incisor and lateral) was considered the most aesthetic, while Smile III (no difference in height between the central and lateral incisors) was the least aesthetic. A significant difference was found between perception of smile II (difference of 1 mm in relation to the central incisor and lateral) and age groups ( P < 0.005), with 31% (n = 13) of individuals aged 45–64 years considering smile II as not pleasant, 4.4% (n = 2) aged 15–24 years and 8.3% (n=4) aged 25–44 years and 4.4% (n = 2) aged 15–24 years considering the same. A significant difference was also found in relation to educational background of the assessor and smile perception, with smile III ( P = 0.0441) and smile IV ( P = 0.0053) considered the most aesthetic ( P = 0.0116). Conclusion: The smile considered most attractive among the sample was that with 1.5 mm steepness between the central and lateral incisors, while a flat smile arc was considered least attractive.

scholarly journals Investigating social media statistics of usage and its determinant factors in students of Kurdistan University of Medical Sciences in 2018: A cross-sectional study.

2021 ◽  
Author(s):  
Hiva Bozorgpanah ◽  
Ghobad Moradi ◽  
Pourya Bahrami ◽  
Elham Noori ◽  
Mohammad Pourya Ezati ◽  
...  
Keyword(s):  
Social Media ◽  
Age Groups ◽  
Cross Sectional Study ◽  
Medical Sciences ◽  
Cross Sectional ◽  
Determinant Factors ◽  
Significant Difference ◽  
Improper Use ◽  
Spss Software ◽  
Use Of Social Media

Abstract Background and Aim: the present study was conducted to investigate social media statistics of usage and its determinant factors in students of Kurdistan University of Medical Sciences in 2018.Materials and Methods: This study is a cross-sectional-analytical study. The total of 380 students of Kurdistan University of Medical Sciences were randomly selected by Probability Proportional to Size Sampling method, from the lists of the students in each faculty. The data collection tool was a researcher-made questionnaire, organized in four sections. Data analysis was done using descriptive and inferential statistics by SPSS software version 21.Results: Based on the results of this study 373 (98.2%) of the sample students were on social media, which the most popular ones among them were Telegram (94.1%), Instagram (78.8%) and Whatsapp (61.9%), respectively. The average time spent on social media among students was reported to be 2.78 hours per day and is a significant difference between age groups in terms of attitudes toward using social media (P = 0.031).Conclusion: the results of this study suggested that the majority of students use social media. Since improper use of social media can exert unfortunate effects on different aspects of life of students, and negatively influence their education.

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