scholarly journals Increased Circulatory Interleukin-17A Levels in Patients with Progressive and Leukotrichial Vitiligo

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Thai Van Thanh Le ◽  
Huy Ngoc Phan ◽  
Tran Ngoc Dang ◽  
Le Duy Pham
Keyword(s):  
Lupus Erythematosus ◽  
Age Of Onset ◽  
Treatment Options ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Clinicopathological Characteristics ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Cross Sectional ◽  
Interleukin 17A

Background. Vitiligo is a chronic condition characterized by skin depigmentation. Although not life-threatening, it significantly impacts quality of life. The pathophysiology of vitiligo remains poorly understood, and treatment options are limited. Mounting evidence supports the importance of autoreactive T cells and, particularly interleukin-17A- (IL-17A-) secreting Th17 cells, in vitiligo. IL-17A targeting has been proven successful in various inflammatory dermatological conditions, including psoriasis and lupus erythematosus. Objective. We evaluated the relationship between serum levels of IL-17A and the clinicopathological characteristics of Vietnamese vitiligo patients. Methods. In this cross-sectional study, we analyzed data from 52 nonsegmental vitiligo patients and 50 age- and sex-matched healthy individuals. Serum levels of IL-17A were measured using an enzyme-linked immunosorbent assay. We evaluated the correlation between IL-17A levels and clinical characteristics including leukotrichia, disease duration, vitiligo activity, and body surface area involvement. Results. Patients with progressive vitiligo had significantly higher IL-17A levels than patients with stable vitiligo ( P  = 0.014) or healthy individuals ( P  = 0.002). In addition, serum IL-17A levels were higher in vitiligo patients with leukotrichia than in patients without it ( P  = 0.04). Furthermore, serum IL-17A levels were negatively correlated with age (r = −0.39, P  = 0.004) and age of onset (r = −0.33, P  = 0.016) in vitiligo patients. Conclusions. Higher serum levels of IL-17A in patients with progressive vitiligo and leukotrichia suggest a potential role of IL-17A in melanocyte destruction in the epidermis and the follicular matrix.

scholarly journals Investigation of IL-17A Serum Levels in Patients with Nonmelanoma Skin Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Mehdi Ghahartars ◽  
Fatemeh Sedaghat ◽  
Elham Khajavi ◽  
Amir Ali Nejat ◽  
Mahyar Malekzadeh ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Cell Carcinoma ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Nonmelanoma Skin Cancer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cross Sectional ◽  
Interleukin 17A ◽  
Significant Difference

Background. Role of interleukin 17A (IL-17A) in carcinogenesis and cancer growth is controversial. Although some researches support its antitumor activity, some others suggest that it promotes the growth and development of different types of cancer including skin cancer by activation of STAT3. Although the function of the cytokines such as IL-17A has been extensively studied in various types of cancer, nonmelanoma skin cancer (NMSC) has not received much attention. Therefore, the present study was aimed to investigate the serum levels of IL-17A in NMSC patients. Methods. This cross-sectional study was performed on 60 patients with basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) as well as 57 age-sex matched healthy individuals as control group. Measurement of IL-17A serum levels in both case and control groups was performed by a commercially reliable sandwich enzyme-linked immunosorbent assay (ELISA) kit. Results. In this study, we observed that IL-17A serum levels in NMSC patients were significantly higher than the control group P < 0.001 . Also, both BCC and SCC patients had higher levels of IL-17A in their sera in comparison to the controls ( P = 0.001 and P < 0.001 , respectively). However, there was no significant difference between SCC and BCC patients regarding serum levels of IL-17A. Conclusion. According to our results, it can be concluded that IL-17A may play a role in inducing the growth and progression of NMSC and it can be used as a therapeutic target in these patients in future.

scholarly journals Evaluation of the Blood Level of Adiponectin in Pregnant Adolescents

2021 ◽  
Vol 43 (06) ◽  
pp. 429-435
Author(s):  
Elaine Cristina Rocha Pádua ◽  
Silvia Daher ◽  
Isa de Pádua Cintra Sampaio ◽  
Edward Araujo Júnior ◽  
Cristina Falbo Guazzelli
Keyword(s):  
Serum Levels ◽  
Cross Sectional Study ◽  
Serum Adiponectin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sectional Study ◽  
Pregnant Adolescents ◽  
Cross Sectional ◽  
Adolescent Group ◽  
Gestational Weight ◽  
Intergroup Comparison

Abstract Objective To evaluate serum levels of adiponectin in pregnant adolescents between 30 and 36 weeks of gestation. Method: A prospective cross-sectional study enrolled 67 normal pregnant women between 30 and 36 weeks of gestation and eutrophic (body mass index [BMI]: 18.5–25 kg/m2), of which 36 were adolescents (< 20 years old) and 31 adults (≥ 20 years old). Serum adiponectin levels were determined by enzyme-linked immunosorbent assay (ELISA). The t-student or Mann-Whitney tests were used for intergroup comparison. Results Pregnant adolescents showed significantly higher serum adiponectin concentrations compared with pregnant adults (p = 0.04). No differences were observed in adiponectin levels in younger pregnant adolescents (< 16 years old) compared with older pregnant adolescents (≥ 16 years old). Adiponectin values were divided into 3 subgroups: < 3,000 ng/mL, between 3,000 and 5,000 ng/mL, and > 5,000 ng/mL. Birthweight was significantly higher in women > 5,000 ng/mL when compared with < 3,000 ng/mL in the adolescent group. No association between pregestational adiponectin levels and BMI, gestational weight gain, and gestational age was observed; however, there was a positive relation with birthweight (p = 0.0239). Conclusion Serum adiponectin values in pregnant adolescents between 30 and 36 weeks of gestation were higher compared with pregnant adults; however, no differences between younger and older pregnant adolescents were observed.

Serum levels of ARMS2, COL8A1, RAD51B, and VEGF and their correlations in Age-Related Macular Degeneration

2021 ◽  
Vol 18 ◽  
Author(s):  
Priya Battu ◽  
Kaushal Sharma ◽  
Manjari Rain ◽  
Ramandeep Singh ◽  
Akshay Anand
Keyword(s):  
Macular Degeneration ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Statistical Correlation ◽  
Age Related Macular Degeneration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cross Sectional ◽  
Age Related ◽  
Study Results

Background: Many factors including genetic and environmental are responsible for the incidence of age-related macular degeneration (AMD). However, its pathogenesis has not been clearly elucidated yet. Objective: This study aimed to estimate the Age-Related Maculopathy Susceptibility 2 (ARMS2), Collagen type VIII Alpha 1 chain (COL8A1), Rad 51 paralog(RAD51B), and Vascular Endothelial Growth Factor (VEGF) protein levels in serum of AMD and control participants and to further investigate their correlation to understand AMD pathogenesis. Methods: For this cross-sectional study, 31 healthy control and 57 AMD patients were recruited from Advanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India. A blood sample was taken and serum was isolated from it. ELISA(enzyme-linked immunosorbent assay)was used for the estimation of proteins in the serum of patients. Results: ARMS2 and COL8A1 levels were significantly elevated in the AMD group than in the control group. The highest levels of ARMS2, COL8A1, and VEGF proteins were recorded for the wet AMD sub-group. The study results endorsed significant positive correlation between these following molecules; ARMS2 and COL8A1 (r=0.933, p<0.0001), ARMS2 and RAD51B (r=0.704, p<0.0001), ARMS2 and VEGF (r=0.925, p<0.0001), COL8A1 and RAD51B (r=0.736, p<0.0001), COL8A1 and VEGF (r=0.879, p<0.0001),and RAD51B and VEGF (r=0.691, p<0.0001). Conclusion: The ARMS2 and COL8A1 levels were significantly higher and RAD51B was significantly lower in the AMD group than controls. Also, a significant statistical correlation was detected between these molecules, indicating that their interaction may be involved in the pathogenesis of AMD.

scholarly journals Association between vitamin D, oestradiol and interferon-gamma in female patients with inactive systemic lupus erythematosus: A cross-sectional study

2017 ◽  
Vol 46 (3) ◽  
pp. 1162-1171 ◽  
Author(s):  
Visnja Kokic ◽  
Dusanka Martinovic Kaliterna ◽  
Mislav Radic ◽  
Leida Tandara ◽  
Dijana Perkovic
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Vitamin D ◽  
Lupus Erythematosus ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sectional Study ◽  
Systemic Lupus ◽  
Cross Sectional ◽  
Female Patients ◽  
Hydroxyvitamin D

Objectives To investigate possible associations between 25-hydroxyvitamin D3 (25(OH)D3), oestradiol (E2) and IFN-gamma (IFNγ) in female patients with inactive systemic lupus erythematosus (SLE). Methods Female patients with inactive SLE and age-matched healthy controls were recruited into this cross-sectional study. Serum concentrations of 25(OH)D3, E2 and IFNγ were measured by radioimmunoassay with gamma-counters and enzyme-linked immunosorbent assay. Results 36 patients and 37 controls were enrolled. In patients with SLE, the concentration of 25(OH)D3 was lower and E2 was higher compared with controls. In vitamin D deficient (i.e., 25(OH)D3≤20 ng/ml) patients, IFNγ was 150% higher compared with patients with 25(OH)D3>20 ng/ml and controls. The concentration of E2 was higher in all patients compared with controls independently of the vitamin D level. A difference was found between patients and controls in the correlation of 25(OH)D3 with E2 and a positive correlation was found between E2 and IFNγ in all participants. Conclusions Our results suggest that E2 may have a strong modulating effect on vitamin D function which is significant only at low concentration of E2.

scholarly journals Sero-Epidemiological Study of Hepatitis E Virus among Thalassemia as High Risk Patients: A Cross-Sectional Survey in Jahrom, Southern, Iran

2016 ◽  
Vol 8 (9) ◽  
pp. 245 ◽  
Author(s):  
Abdolreza Sotoodeh Jahromi ◽  
Abbas Ahmadi-vasmehjani ◽  
Hassan Zabetian ◽  
Hossein Hakimelahi ◽  
Alireza Yusefi ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Age Groups ◽  
Serum Levels ◽  
Hepatitis E ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Significant Difference ◽  
Ig G

<p>Hepatitis E virus (HEV) could be cause of viral hepatitis in the developing countries and cause severe epidemics. According to other studies, blood transfusion as a probable route of HEV infection has been suggested. An infection with hepatitis agents such as HEV causes active liver failure in multi-transfusion patients in particular thalassemia. The purpose of this study determines the seropositivity of anti-HEV antibodies in thalassemia individuals in Jahrom. In a cross-sectional study, sera from 110 thalassemia were collected between 2013 and 2014. Enzyme-linked immunosorbent assay (ELISA) method was performed to detection of anti-HEV antibodies. Individuals’ data were collected such as, demographic and clinical, for statistical analysis. Our results show that 10% and 1.8% of the enrolled patients were HEV Ig-G and Ig-M positive antibodies respectively. In addition, there was statiscally significant difference in age groups for prevalence of anti-HEV Ig-G (P = 0.01). Also the serum levels of liver enzymes such as ALT and AST in the HEV Ig-G and Ig-M positive samples were significantly higher than anti-HEV negative samples. But there were no significant difference between sex and splenectomy with anti-HEV positive samples. The results indicate more study are needed to assess HEV screening of blood products to these patients that those have a probably risk of exposure to HEV especially in higher years old. </p>

scholarly journals Anti-Mullerian Hormone Levels in Regularly Menstruating Nigerian Women

2021 ◽  
Vol 7 (2) ◽  
pp. 95-106
Author(s):  
OO Olawale ◽  
VO Dada ◽  
FM Abbiyesuku ◽  
OO Eluyera ◽  
EW Olooto ◽  
...  
Keyword(s):  
Serum Levels ◽  
Significant Negative Correlation ◽  
Cross Sectional Study ◽  
Reproductive Age ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Nigerian Women ◽  
Healthy Women ◽  
Caucasian Americans ◽  
The North

Background: Studies have shown that serum levels of Anti-Mullerian Hormones (AMH) decrease with age as it is also an early and sensitive marker of ovarian reserve in women in the North American, European and Asian regions. Various research works have also generated data about AMH in the Caucasian, Americans and Asians There was a need to compare these known data with African data. Objectives: To assess the serum levels of AMH in healthy women of reproductive age and determine the relationship between AMH, age, Body Mass Index, parity and menstrual cycle in healthy regularly menstruating women. Methods: A cross-sectional study of 200 apparently healthy women aged 21-45 years was carried out between January and May 2014. Serum AMH and FSH levels were measured in the participants using Enzyme-Linked Immunosorbent Assay. Results: The median AMH value was 4.07ng/mL, while the median FSH value was 9.65mIU/mL. The reference 90% CI of AMH was 0.60 -9.71 ng/ml. There was a significant negative correlation between serum level of AMH and age (r = - 0.718, p<0.001). Conclusion: The serum AMH levels gradually declined throughout the reproductive lifespan of a woman.

scholarly journals Galectin-1, The New Player in Staging and Prognosis of COVID-19

2021 ◽  
Author(s):  
Sofija Sekulic ◽  
Nevena Gajovic ◽  
Milena Jurisevic ◽  
Marina Jovanovic ◽  
Biljana Popovska Jovicic ◽  
...  
Keyword(s):  
Proinflammatory Cytokines ◽  
Virus Disease ◽  
Radiographic Finding ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Atypical Pneumonia ◽  
Tissue Destruction ◽  
Roc Curve Analysis ◽  
Cross Sectional

Abstract Background A new virus from the group of coronaviruses was identified as the cause of atypical pneumonia and called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and disease called Corona Virus Disease (COVID-19). During the cytokine storm, the main cause of the death, proinflammatory cytokines are released which stimulate further tissue destruction. Galectin-1 is pleiotropic cytokine involved in many immune and inflammatory processes and its role in COVID-19 is still unknown. Objective The aim of this study was to determine systemic values of galectin-1 and correlations between gal-1 and proinflammatory cytokines and clinical parameters during COVID-19 progression. Design Observational and cross-sectional study. Patients 210 COVID-19 patients were included and divided into mild, severe or critical group according to COVID-19 severity. Main Measures Serum levels of IL-1β, IL-6, IL-10, IL-23, IL-33 and galectin-1 were measured using sensitive enzyme-linked immunosorbent assay (ELISA) kits. Key Results Systemic levels of IL-1β, IL-6, IL-10, IL-23, IL-33 and galectin-1 were significantly higher in stage III of COVID-19 patients compared to stage I and II. There were no significant differences in ratio between Galectin-1 and IL-10 with proinflammatory cytokines. Positive correlation was detected between Gal-1 and IL-1β, IL6, IL-10, IL-23 and IL-33. Gal-1 positively correlated with chest radiographic finding, dry cough and headache and negatively correlated with normal breathing sound. Conclusions Linear regression model and ROC curve analysis point on Gal-1 as significant predictors for COVID-19 severity. Presented results implicate on Gal-1 and IL-10 dependent immunomodulation. The precise mechanism of Gal-1 effect in COVID-19 and its potential as a stage marker of disease severity is still to be clarified.

scholarly journals Bone status and aortic calcifications in chondrocalcinosis patients

2020 ◽  
Vol 28 (2) ◽  
pp. 195-204
Author(s):  
Paulina Vele ◽  
Ciprian Nicolae Silaghi ◽  
Laura Otilia Damian ◽  
Siao-Pin Simon ◽  
Alexandra Craciun ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Bone Turnover Markers ◽  
Statistical Significance ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Mineral Density ◽  
Cross Sectional ◽  
Turnover Markers

AbstractAim: We aimed to examine the association between several circulating bone turnover markers [ osteocalcin (OC), osteoprotegerin (OPG), beta-CrossLaps (β-CTx)], hip and spine bone mineral density (BMD) and abdominal aortic calcification (AAC) in patients with chondrocalcinosis (CC).Methods: Thirty-six patients with CC and thirty-seven controls were consecutively enrolled in this pilot case-control, cross-sectional study. The following parameters were assessed: serum levels of OC, OPG and β-CTx by enzyme-linked immunosorbent assay (ELISA); hip and spine BMD by dual-energy X-ray absorptiometry and AAC score by lateral radiography.Results: Patients with CC had higher levels of serum bone turnover markers and AAC score than the control group: OC [6.5 (3.5-9.9) vs 4.5 (2.6-7.2) ng/ml; p=0.05], OPG [(7.7 (6.2-9.4) vs 6.5 (5.5-8.12) pmol/ml; p=0.02], β-CTx [6078 (5870-6171) vs 5851 (5465-6109) pg/ml; p=0.02] and AAC score (3.6±6.2 vs 0.5±2; p=0.006). Conversely, even if statistical significance was not reached, hip and spine BMD was lower in patients with CC. Additionally, we found a positive correlation between OPG and AAC, but also between OPG and osteoporosis in patients with CC.Conclusion: Patients with CC are characterized by higher circulating OC, OPG and β-CTx. The presence of AAC was more common in patients with CC, being only associated with serum OPG.

scholarly journals The Usefulness of Endothelial Cell Adhesion Molecules and Anti-C1q Antibody in Monitoring Systemic Lupus Erythematosus Disease Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Hasni Mahayidin ◽  
Nurul Khaiza Yahya ◽  
Wan Syamimee Wan Ghazali ◽  
Asmahan Mohd Ismail ◽  
Wan Zuraida Wan Ab Hamid
Keyword(s):  
Disease Activity ◽  
Lupus Erythematosus ◽  
Activity Index ◽  
Inverse Correlation ◽  
Disease Activity Index ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Positive Correlations ◽  
Global Disease Activity

Objectives. The study was conducted to determine the correlation of ICAM-1, VCAM-1, and anti-C1q antibody levels with SLE disease activity index (SLEDAI) and standard SLE disease activity immunological markers (anti-dsDNA and sera C3 and C4). Study Design. This was a cross-sectional study. Materials and Methods. Blood samples were obtained from 95 SLE patients (45 active SLE and 50 nonactive SLE) and 50 controls. The subjects were assessed using SLEDAI and score of more than five is determined as having active SLE. The sera were tested for serum ICAM-1, VCAM-1, and anti-C1q (ELISA), anti-dsDNA (CLIFT), serum C3, and serum C4 (immunonephelometry). Results. Anti-dsDNA and anti-C1q antibody showed good positive correlations with SLEDAI (r=0.529, P<0.001 and r=0.559, P<0.001, resp.). VCAM-1 and sera C3 and C4 showed fair correlation with SLEDAI (r=0.294, P=0.004; r=-0.312, P=0.002; and r=-0.382, P<0.001, resp.). ICAM-1 level showed no significant correlation with SLEDAI (P=0.062). There were significant correlations of VCAM-1 and anti-C1q antibody with anti-dsDNA (r=0.226, P=0.006 and r=0.511, P<0.001, resp.). VCAM-1 showed poor inverse correlation with serum C3 (r=-0.183, P=0.028) and fair inverse correlation with serum C4 (r=-0.251, P=0.002). Anti-C1q antibody demonstrated fair inverse correlation with both sera C3 and C4 (r=-0.420, P≤0.001 and r=-0.398, P<0.001, resp.). However, ICAM-1 showed no significant correlation with anti-dsDNA and sera C3 and C4 (P=0.259, P=0.626 and P=0.338, resp.). Conclusions. The serum levels of anti-C1q antibody in SLE patients showed the best correlation with the SLEDAI and standard immunological tests for SLE disease activity. These data support that anti-C1q antibody is a useful marker for monitoring SLE global disease activity. The potential of VCAM-1 needs further confirmation.

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