scholarly journals Therapeutic Effect of Seawater Pearl Powder on UV-Induced Photoaging in Mouse Skin

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Siyin Han ◽  
Delun Huang ◽  
Taijin Lan ◽  
Yongpei Wu ◽  
Yingbiao Wang ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
Mouse Skin ◽  
Mapk Signaling ◽  
Collagen I ◽  
Emission Spectrometry ◽  
Atomic Fluorescence Spectrometry ◽  
Histopathological Analysis ◽  
Skin Damage ◽  
Photoaged Skin ◽  
Matrix Metalloproteinase 1

The objective of this study was to investigate the therapeutic effect of seawater pearl powder (SPP) on ultraviolet (UV) irradiation-induced photoaging in mouse skin. The protein and trace elements in SPP were detected by liquid chromatography-mass spectrometry, atomic fluorescence spectrometry, and inductively coupled plasma-atomic emission spectrometry. The effect of SPP on treating skin damage resulting from UV-induced photoaging was observed by gross physical appearance and histopathological analysis. Oxidative stress and melanin synthesis were analyzed using biochemical method. Western blotting was applied to analyze the phosphorylation and expression levels of matrix metalloproteinase-1 (MMP-1), collagen I, and proteins involved in the mitogen-activated protein kinase (MAPK) signaling pathways (p38, ERK, and JNK). The results show that SPP has a significant therapeutic effect on UV-induced photoaging of skin and improves and restores appearance and tissue structure of mouse skin. The major mechanism may be related to reduction of expression level of MMP-1 and enhancement of collagen I production via inhibition of MAPK signaling pathway after scavenging of excess reactive oxygen species (ROS) in the UV-induced photoaged skin of mice. Meanwhile, it may also be involved in reducing melanin content by inhibiting tyrosinase activity after scavenging excess ROS in the UV-induced photoaged skin of mice. Therefore, SPP could be a good substance to treat photoaging skin. Taking cost-effectiveness and efficacy into consideration, the optimal concentration of SPP for treating photoaging skin could be 100 mg/g.

scholarly journals HYBID (alias KIAA1199/CEMIP) and hyaluronan synthase coordinately regulate hyaluronan metabolism in histamine-stimulated skin fibroblasts

2020 ◽  
Vol 295 (8) ◽  
pp. 2483-2494
Author(s):  
Hiroyuki Yoshida ◽  
Mika Aoki ◽  
Aya Komiya ◽  
Yoko Endo ◽  
Keigo Kawabata ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Fold Increase ◽  
Histamine Receptor ◽  
Skin Fibroblasts ◽  
Dependent Manner ◽  
Skin Damage ◽  
Photoaged Skin ◽  
Molecular Sizes ◽  
Ha Synthase ◽  
Hyaluronan Binding

The immune-regulatory compound histamine is involved in the metabolism of the essential skin component hyaluronan (HA). We previously reported that histamine up-regulates the expression of HYBID (hyaluronan-binding protein involved in hyaluronan depolymerization, also called CEMIP or KIAA1199), which plays a key role in HA degradation. However, no information is available about histamine's effects on HA synthase (HAS) expression, the molecular sizes of HA species produced, and histamine receptors and their signaling pathways in skin fibroblasts. Moreover, histamine's effects on photoaged skin remain elusive. Here, we show that histamine increases HA degradation by up-regulating HYBID and down-regulating HAS2 in human skin fibroblasts in a dose- and time-dependent manner and thereby decreases the total amounts and sizes of newly produced HA. Histamine H1 blocker abrogated the histamine effects on HYBID up-regulation, HAS2 suppression, and HA degradation. Histamine H1 agonist exhibited effects on HA levels, composition, and breakdown similar to those of histamine. Of note, blockade of protein kinase Cδ or PI3K–Akt signaling abolished histamine-mediated HYBID stimulation and HAS2 suppression, respectively. Immunohistochemical experiments revealed a significant ∼2-fold increase in tryptase-positive mast cells in photoaged skin, where HYBID and HAS2 expression levels were increased and decreased, respectively, compared with photoprotected skin. These results indicate that histamine controls HA metabolism by up-regulating HYBID and down-regulating HAS2 via distinct signaling pathways downstream of histamine receptor H1. They further suggest that histamine may contribute to photoaged skin damage by skewing HA metabolism toward degradation.

Analytical methods for the quantification of selenium species in biological matrix: Where are we?

2021 ◽  
Vol 02 ◽  
Author(s):  
Rama Alhasan ◽  
Caroline Perrin-Sarrado ◽  
Claus Jacob ◽  
Caroline Gaucher
Keyword(s):  
Analytical Methods ◽  
Inductively Coupled Plasma ◽  
Hydride Generation ◽  
Essential Element ◽  
The Body ◽  
Cutting Edge ◽  
Emission Spectrometry ◽  
Atomic Fluorescence Spectrometry ◽  
Selenium Species ◽  
Inductively Coupled

Objective: Over the years, scientific investigations have proven the importance of selenium as an essential element for mammals, emphasizing its activity against many diseases and even its prophylactic effects. It is also established now that a malconsumption of selenium can be harmful. Therefore, the nature and the concentration of selenium and its derivatives found in the diet, the body, and even in the environment, for example, in the soil, should be determined carefully. Methods: In this review, analytical methods for speciation and determination of selenium concentrations in biological samples are summarized. Results: Methods ranging from routine to cutting-edge are explored, focusing on their analytical characteristics, such as specificity for discrete selenium species, sensitivity, accuracy, reproducibility, and skills required. Conclusion: There are already numerous studies regarding the analysis of selenium species. Beyond the method employed for actual measurements, we propose to review the preanalytic steps for sample handling in biological matrices, which directly affect results that will be more accurate with careful pretreatment. Furthermore, to reach better outcomes in terms of the identification of selenium species, different combinations of techniques might be the answer. We highlight here the last and the cutting-edge methods to identify and quantify selenium such as, high-performance liquid chromatography combined to inductively coupled plasma mass spectrometry (HPLC-ICP-MS), hydride generation atomic absorption spectrometry (HG-AAS), hydride-generation combined to atomic fluorescence spectrometry (HG-AFS), or to inductively coupled plasma optical emission spectrometry (HG-ICP-OES). This review emphasizes the importance of such investigations and the need to achieve reliable, safe, and effective quantification and methods of determination.

scholarly journals EFFICACY OF TRIDHAM AND 1,2,3,4,6-PENTA-O-GALLOYL-β-D-GLUCOSE IN REVERSING LIPID PEROXIDATION LEVELS AND MITOCHONDRIAL ANTIOXIDANT STATUS IN 7,12-DIMETHYLBENZENEANTHRACENE (DMBA) INDUCED BREAST CANCER IN SPRAGUE-DAWLEY RATS

2016 ◽  
Vol 8 (9) ◽  
pp. 288 ◽  
Author(s):  
Stalin Ramakrishnan ◽  
Karthick Dharmalingam ◽  
Sachidanandham T Panchanatham ◽  
Shanthi Palanivelu
Keyword(s):  
Lipid Peroxidation ◽  
Therapeutic Effect ◽  
Mammary Carcinoma ◽  
Antioxidant Status ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Sprague Dawley ◽  
Standard Drug ◽  
Gastric Intubation ◽  
Group I

<p><strong>Objective: </strong>To determine the effect of <em>Tridham</em> (TD) and 1,2,3,4,6-penta-O-galloyl-β-d-glucose(PGG) on lipid peroxidation levels and mitochondrial antioxidants status in experimental mammary carcinoma.</p><p><strong>Methods</strong>:<strong> </strong><em>Elaecoarpus ganitrus </em>(fruits), <em>Terminalia chebula </em>(seed coats), <em>Prosopis cineraria </em>(leaves)<em>, </em>adult female albino rats of Sprague-Dawley strain weighing 170–190 g and 7,12-dimethylbenzeneanthracene (DMBA) were used for this study. Group I control rats, Group II rats mammary carcinoma induced with DMBA (25 mg in 1 ml olive oil) by gastric intubation. Group III, IV and V DMBA induced rats were treated with TD (400 mg/kg. b. wt/day), PGG (30 mg/kg. b. wt/day) and standard drug, Cyclophosphamide (30 mg/kg. b. wt/day), respectively for 48 d by gastric intubation. Group VI and VII rats served as TD and PGG treated controls, respectively for 48 d by gastric intubation. At the end of the experimental period, the rats were anaesthetized and sacrificed. Mammary glands were isolated and used for biochemical assays and histopathological evaluation.</p><p><strong>Results: </strong>In rats with cancer, the lipid peroxide levels (LPO) were significantly increased and mitochondrial antioxidant levels were decreased. Treatment with TD and PGG decreased LPO levels and increased mitochondrial antioxidant status in mammary carcinoma bearing rats. Histopathological analysis also confirmed the therapeutic effect of TD and PGG. No significant adverse effect was observed in sole drug treated group of rats.</p><p><strong>Conclusion: </strong>TD and PGG have definite therapeutic effect in experimental mammary carcinoma and inhibit growth of cancer cells by restoring mitochondrial antioxidant status and energy metabolism to normal states.</p>

scholarly journals Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression

2018 ◽  
Vol 19 (9) ◽  
pp. 2503 ◽  
Author(s):  
Ah-Ram Han ◽  
Tae-Gyu Lim ◽  
Young-Ran Song ◽  
Mi Jang ◽  
Young Rhee ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Radical Scavenging ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Dermal Fibroblasts ◽  
Ros Generation ◽  
Skin Damage ◽  
Opuntia Humifusa ◽  
Matrix Metalloproteinase 1 ◽  
Solar Ultraviolet

Opuntia humifusa is a type of cactus whose fruits have been used in folk medicine for the treatment of several diseases. In the present study, we aimed to determine whether O. humifusa fruit water extract (OHE) has inhibitory effects against solar ultraviolet (sUV)-induced matrix metalloproteinase-1 (MMP-1) expression. In ex vivo human skin, we found that OHE suppressed sUV radiation-induced MMP-1 expression. The inhibitory effect of OHE was confirmed in human dermal fibroblasts. OHE treatment reduced sUV-induced MMP-1 expression by suppressing reactive oxygen species (ROS) generation and phosphorylation of c-Jun, a component of transcription factor activator protein 1 (AP-1). On the other hand, OHE recovered the tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and type 1 collagen production attenuated by sUV. As upstream signaling pathways for AP-1, MKK4-JNK, MEK-ERK, and MKK3/6-p38 phosphorylation were downregulated by OHE treatment. In addition, OHE exhibited DPPH radical scavenging activity. These findings demonstrate that OHE has a preventive effect against sUV-induced skin damage via suppression of pathways triggered by ROS.

Role of Biomarkers in Incisional Hernias

2007 ◽  
Vol 73 (6) ◽  
pp. 561-567 ◽  
Author(s):  
J.R. Salameh ◽  
Ladawn M. Talbott ◽  
Warren May ◽  
Bashar Gosheh ◽  
Parminder J.S. Vig ◽  
...  
Keyword(s):  
Biological Activities ◽  
Transcript Level ◽  
Collagen I ◽  
Type I ◽  
Western Blots ◽  
Incisional Hernias ◽  
Matrix Metalloproteinase 1 ◽  
Previous Laparotomy ◽  
Polymerase Chain

Incisional hernias represent one of the most common complications of laparotomies. Previous investigations have suggested that a disorder in collagen fiber structure and production level may be an important pathologic cause of abdominal wall hernias. We hypothesized that a cross-examination of multiple extracellular matrix biomarkers might identify underlying defects contributing to the development of hernias. We examined two patient populations: patients with incisional hernias (presenting for hernia repair) and patients with no hernia after previous laparotomy (undergoing a second laparotomy). Patients with previous wound infections, open abdomens, or on steroids were excluded. Fascia samples were obtained from all patients at the time of their second operation and they were studied. Western blots and reverse transcriptase-polymerase chain reaction were used to determine the ratio of type I, III, and IV collagens, as well as matrix metalloproteinase 1 (MMP1) and MMP2 in both groups. Values of P < 0.05 were considered statistically significant. At the protein level, collagen I/III ratio was slightly decreased in patients with incisional hernias compared with those with no hernia, whereas it was significantly decreased at the mRNA transcript level (0.49 vs 1.03, P < 0.01, respectively). The MMP-1 mRNA transcripts were not different in incisional hernia (IH) versus nonincisional hernia, but the MMP-2 level was significantly increased in patients with IH. Reduced collagen I/III and MMP-1/MMP-2 ratios in IH might be consequence of the biological activities between key elements participating in the development of IH after laparotomies. The potential role of MMP-2–specific inhibitors in preventing IH is of significance for future studies.

Preclinical Radiobiology with Pions: RBE ? Values for Mouse Skin Damage as a Function of Single Doses of Pions

1979 ◽  
Vol 16 (3) ◽  
pp. 289-294 ◽  
Author(s):  
E. M. Fr�hlich ◽  
H. Blattmann ◽  
L. Pfister ◽  
I. Cordt ◽  
J. Zehnder ◽  
...  
Keyword(s):  
Mouse Skin ◽  
Skin Damage

scholarly journals Hydrangea serrata (Thunb.) Ser. Extract Attenuate UVB-Induced Photoaging through MAPK/AP-1 Inactivation in Human Skin Fibroblasts and Hairless Mice

Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 533 ◽  
Author(s):  
Hee-Soo Han ◽  
Ji-Sun Shin ◽  
Da-Bin Myung ◽  
Hye Ahn ◽  
Sun Lee ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Molecular Mechanisms ◽  
Mapk Signaling ◽  
Collagen Degradation ◽  
Hot Water ◽  
Mrna Levels ◽  
Skin Damage ◽  
Hairless Mice ◽  
Skin Photoaging ◽  
Mapk Signaling Pathways

Skin photoaging is mainly caused by exposure to ultraviolet (UV) light, which increases expressions of matrix metalloproteinases (MMPs) and destroys collagen fibers, consequently inducing wrinkle formation. Nutritional factors have received scientific attention for use as agents for normal skin functions. The aim of this study was to investigate the effect of hot water extracts from the leaves of Hydrangea serrata (Thunb.) Ser. (WHS) against ultraviolet B (UVB)-induced skin photoaging and to elucidate the underlying molecular mechanisms in human foreskin fibroblasts (Hs68) and HR-1 hairless mice. WHS recovered UVB-reduced cell viability and ameliorated oxidative stress by inhibiting intracellular reactive oxygen species (ROS) generation in Hs68 cells. WHS rescued UVB-induced collagen degradation by suppressing MMP expression, and reduced the mRNA levels of inflammatory cytokines. These anti-photoaging activities of WHS were associated with inhibition of the activator protein 1 (AP-1), signal transduction and activation of transcription 1 (STAT1), and mitogen-activated protein kinase (MAPK) signaling pathways. Oral administration of WHS effectively alleviated dorsal skin from wrinkle formation, epidermal thickening, collagen degradation, and skin dehydration in HR-1 hairless mice exposed to UVB. Notably, WHS suppressed UVB activation of the AP-1 and MAPK signaling pathways in dorsal mouse skin tissues. Taken together, our data indicate that WHS prevents UVB-induced skin damage due to collagen degradation and MMP activation via inactivation of MAPK/AP-1 signaling pathway.

Sign in / Sign up

Export Citation Format

Share Document