scholarly journals Determination of the Chemical Stability of Cyanocobalamin in Medical Food by a Validated Immunoaffinity Column-Linked HPLC Method

2022 ◽  
Vol 2022 ◽  
pp. 1-8
Author(s):  
Wonjong Lee ◽  
Yoon-Bok Lee ◽  
Moon Haeng Huh ◽  
Jae Kwon Choi
Keyword(s):  
Hplc Method ◽  
The Body ◽  
Coefficient Of Determination ◽  
Analysis Method ◽  
Food Matrix ◽  
Medical Food ◽  
A Value ◽  
Medical Foods ◽  
The Stability

Cyanocobalamin, which plays an essential role in the body, is a synthetic form used in medical food. This present study aimed to develop an HPLC analysis method for determination cyanocobalamin and investigate the stability of cyanocobalamin in medical food. Validation of the developed method for cyanocobalamin was evaluated with linearity, LOD, LOQ, and accuracy. The linearity of this method was calculated with a value of the coefficient of determination (R2) ≥ 0.999. LOD and LOQ were 0.165 and 0.499 μg/kg, respectively. The recovery of medical food matrixes for accuracy was more than 97.63%. The validated method was applied for determining cyanocobalamin from medical foods. The developed method was used to examine the additives for cyanocobalamin protection. Ferric chloride and sorbitol alleviated cyanocobalamin degradation from heat and ascorbic acid. Especially, sorbitol showed a superior protective effect during the medical food production process. Therefore, this study suggests that sorbitol is a sweetener additive that prevents cyanocobalamin degradation by heat and the food matrix in medical food processing.

scholarly journals Determination of Energy Absorption on Seat Regions of Footwear for the Obese

2019 ◽  
Vol 27 (4(136)) ◽  
pp. 76-80
Author(s):  
Selvaraj Mathivanan ◽  
Ranganathan Mohan ◽  
Palanisami Balachander
Keyword(s):  
Energy Absorption ◽  
Slope Angle ◽  
The Body ◽  
Coefficient Of Determination ◽  
Predictive Equation ◽  
Absorbed Energy ◽  
Heel Height ◽  
Therapeutic Benefits ◽  
A Value

Ideal footwear with a design configuration is necessitated as a tool of rehabilitation to render therapeutic benefits, especially for obese individuals who are experiencing greater risk during locomotion. Energy absorption is found to depend on variables like heel height, slope angle and load exerted by the body mass index. Statistical analysis was carried out to formulate a predictive equation for absorbed energy. It was found that a heel height of 30 mm offers an optimum base for further design of footwear. A coefficient of determination (R2) with a value of 0.933 indicates that the model fits the experimental data nicely. Hence, 30 mm 20 degree is suggested for designing ideal footwear as it enables load dissipation and energy absorption to render foot-comfort benefits and advantages to the user.

scholarly journals Development and Validation of an Analytical Method for Deacetylasperulosidic Acid, Asperulosidic Acid, Scopolin, Asperuloside and Scopoletin in Fermented Morinda citrifolia L. (Noni)

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 80
Author(s):  
Sun-Il Choi ◽  
Hee-Yeon Kwon ◽  
Im-Joung La ◽  
Yeon-Hui Jo ◽  
Xionggao Han ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Biologically Active ◽  
Morinda Citrifolia ◽  
Coefficient Of Determination ◽  
Analysis Method ◽  
Food Ingredients

Fermentation is a technology that enhances biologically active ingredients, improves the absorption rate and induces the generation of new functional ingredients by the catalytic action of enzyme systems possessed by microorganisms. In this study, changes in the content of five kinds of bioactive compounds (deacetylasperulosidic acid, asperulosidic acid, scopolin, asperuloside and scopoletin) of Morinda citrifolia L. were confirmed by fermentation, and a high-performance liquid chromatography-photodiode array (HPLC-PDA) analysis method for measuring analytes was developed and validated. HPLC method for the determination of five bioactive compounds in Morinda citrifolia L. extracts (MCE) was validated in terms of sensitivity, linearity, selectivity, limit of detection (LOD) and quantification (LOQ), precision and accuracy. The coefficient of determination of the calibration curve for bioactive compounds (1.56–100 μg/mL) showed linearity (R2 ≥ 0.9999). LOD and LOQ were in the range 0.04–0.97 and 0.13–2.95 μg/mL, respectively. The range of intra- and intraday accuracies values (recovery) were 97.5–121.9% and 98.8–118.1%, respectively, and precision value (RSDs) of the bioactive compounds were <4%. In addition, changes in the content of five bioactive compounds in MCE by fermentation were confirmed. These results indicate that the developed fermentation and analysis method could be applied in the development of potential functional food ingredients.

scholarly journals Photostability study of amlodipine besylate tablets packed in primary packaging

2021 ◽  
Vol 10 (1) ◽  
pp. 20-28
Author(s):  
Ivana Savić-Gajić ◽  
Ivan Savić ◽  
Predrag Sibinović ◽  
Valentina Marinković
Keyword(s):  
Mobile Phase ◽  
Limit Of Detection ◽  
Degradation Products ◽  
Hplc Method ◽  
Coefficient Of Determination ◽  
Amlodipine Besylate ◽  
Limit Of Quantification ◽  
First Order Kinetics ◽  
The Given

In this study, the modified stability-indicating RP-HPLC method was validated for quantitative analysis of amlodipine besylate in the presence of its impurity D (3-ethyl 5-methyl 2-[(2-aminoethoxy)methyl]-4-(2-chlorophenyl)-6-methylpyridine-3,5-dicarboxylate). The method was applied for the determination of an analyte in the tablets and irradiated samples packed in the primary packaging (Alu/PVC/PVDC blister packaging). The efficient chromatographic separation was achieved using a ZORBAX Eclipse XDB-C18 column (4.6×250 mm, 5 mm) with isocratic elution of mobile phase which consisted of acetonitrile:methanol:triethylamine solution (15:35:50, v/v/v) (pH 3.0). The flow rate of the mobile phase was 1 mL min-1, while the detection of amlodipine besylate was carried out at 273 nm. Amlodipine besylate and its impurity D were identified at the retention times of 16.529 min and 2.575 min, respectively. The linearity of the method with the coefficient of determination of 0.999 was confirmed in the concentration range of 10 - 75 µg mL-1 for amlodipine besylate. The limit of detection was 0.2 µg mL-1, while the limit of quantification was 0.66 µg mL-1. After UV and Vis radiation of the tablets packed in the primary packaging, the content of amlodipine besylate was reduced by 22.38% and 19.89%, respectively. The presence of new degradation products was not detected under the given chromatographic conditions. The photodegradation of amlodipine besylate followed pseudo-first-order kinetics. Based on the half-life of amlodipine besylate (38.4 days for UV radiation and 43.3 days for Vis radiation), it was concluded that amlodipine besylate in the tablets has satisfactory photostability after its packing in the Alu/PVC/PVDC blister packaging.

scholarly journals Stability-indicating HPLC-PDA assay for simultaneous determination of paracetamol, thiamine and pyridoxal phosphate in tablet formulations

2019 ◽  
Vol 69 (2) ◽  
pp. 249-259 ◽  
Author(s):  
Amir Ali ◽  
Muhammad Makshoof Athar ◽  
Mahmood Ahmed ◽  
Kashif Nadeem ◽  
Ghulam Murtaza ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Pyridoxal Phosphate ◽  
Degradation Products ◽  
Ammonium Phosphate ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Complete Separation ◽  
Coefficient Of Determination ◽  
Tablet Formulations

Abstract With the increased number of multi-drug formulations, there is a need to develop new methods for simultaneous determinations of drugs. A precise, accurate and reliable liquid chromatographic method was developed for simultaneous determination of paracetamol, thiamine, and pyridoxal phosphate in pharmaceutical formulations. Separation of analytes was carried out with an Agilent Poroshell C18 column. A mixture of ammonium phosphate buffer (pH = 3.0), acetonitrile and methanol in the ratio of 86:7:7 (V/V/V) was used as the mobile phase pumped at a flow rate of 1.8 mL min−1. Detection of all three components, impurities and degradation products was performed at the selected wavelength of 270 nm. The developed method was validated in terms of linearity, specificity, precision, accuracy, LOD and LOQ as per ICH guidelines. Linearity of the developed method was found in the range 17.5–30 µg mL−1 for thiamine, 35–60 µg mL−1 for pyridoxal phosphate and 87.5–150 µg mL−1 for paracetamol. The coefficient of determination was ≥ 0.9981 for all three analytes. The proposed HPLC method was found to be simple and reliable for the routine simultaneous analysis of paracetamol, thiamine and pyridoxal phosphate in tablet formulations. Complete separation of analytes in the presence of degradation products indicated selectivity of the method.

scholarly journals Determination of Mefenamic Acid in Pharmaceutical Drugs and Wastewater by RP-HPLC

2017 ◽  
Vol 2 (2) ◽  
pp. 85-88
Author(s):  
Mohammad Anas Alfeen
Keyword(s):  
Mefenamic Acid ◽  
Chromatographic Analysis ◽  
Mobile Phase ◽  
Pharmaceutical Preparations ◽  
Hplc Method ◽  
Treated Wastewater ◽  
Pharmaceutical Drugs ◽  
Analysis Method ◽  
Rp Hplc

This study presents a chromatographic analysis method for the determination of mefenamic acid in pharmaceutical preparations and treated wastewater from the pharmaceutical industry in East Asia and specifically (Syria). An isocratic RP-HPLC method has been developed for determination of Mefenamic acid on a Grace, alltima C18 column (250 x 4.6 mm, 5.0 μm) using a mobile phase consisting of (1%) Triethylamine aqueous buffer adjust pH = 2 by H3PO4 (85%): Methanol: Acetonitrile); (35: 20: 45 v\v\v %) at a flow rate of 2 mL/min. Detection was carried out at 220 nm. Retention time of Mefenamic acid was 7.85 ( ± 0.36) mins. The method was validated with respect to specificity, linearity, accuracy, precision, ruggedness, and robustness.  The proposed method is simple, precise, sensitive, and reproducible and is applicable for quantification of Mefenamic acid in Tablets formulations and Wastewater developed and formulated in our laboratory.

scholarly journals Magnitude Scales in Central Greece

2018 ◽  
Vol 40 (3) ◽  
pp. 1114 ◽  
Author(s):  
G. Kaviris ◽  
P. Papadimitriou ◽  
K. Makropoulos
Keyword(s):  
Goodness Of Fit ◽  
Body Wave ◽  
Velocity Model ◽  
The Body ◽  
Moment Magnitude ◽  
Coefficient Of Determination ◽  
Gulf Of Corinth ◽  
Duration Magnitude ◽  
The Moment

The Gulf of Corinth is one of the most active tectonic rifts around the world. Data used in the present study are obtained by the four digital stations of the Cornet Network which was installed in 1995 around the Eastern Gulf of Corinth. A velocity model was calculated, while the majority of local events were located within the Gulf of Corinth. Main scope of the study is the determination of a reliable earthquake magnitude. Concerning the duration magnitude Mo, a multiple linear regression technique was developed for the determination of the constants α, β and γ with very satisfactory values of errors. The coefficient of determination (goodness of fit) R2 was found equal to 0.99. Following, the moment magnitude Mw, which is considered to be the most reliable magnitude scale, was determined. Spectral analysis was applied for the calculation of the seismic moment M0 and a seismic catalogue was created. After the determination of the moment magnitude Mw and of the duration magnitude MD for the same dataset, a relationship between them was obtained, according to which Mw is systematically larger than Mjy Relationships between these magnitudes, the local magnitude ML and the body wave magnitude mb  were also obtained.

scholarly journals KAJIAN STRUKTUR CHECK DAM 3 BATANG HULU KURANJI KOTA PADANG STUDY OF CHECK DAM STRUCTURE 3 BATANG HULU KURANJI PADANG CITY

2021 ◽  
Vol 3 (4) ◽  
pp. 15-23
Author(s):  
Silta Yulan Nifen ◽  
Afif Dzaky Almy
Keyword(s):  
Rainy Season ◽  
The Body ◽  
Dam Construction ◽  
Check Dam ◽  
Observation Station ◽  
Safety Coefficient ◽  
A Value ◽  
Sediment Flow ◽  
The Stability ◽  
The City

Batang Kuranji is a river located in Kota Padang. The high rainfall and human factors that cause changes in characteristics, especially in the upstream area make the water from the flow of kuranji stems in the rainy season often overflows, and cause flash floods, therefore built Check Dam at the head of the river batang kuranji to prevent the shallowing of the riverbed. Thisresearch aims toreview the structure of the Check Dam 3 building on Batang Kuranji in the city of Padang. This study refers to SNI 2851:2015 with rainfall data for 15 years used from 2005 to 2019, with batu busuk observation station and rice fields obtained from PSDA. Luas DAS is obtained from ArcGIS Applications. Dari hydroligi analysis obtained rainfall plan (R100th) 153,152 m3/dt with Gumbel method, Discharge flood plan for the 100 year anniversary period used Haspers method obtained (Q100th) 165.19 m3/dt. The type of Check Dam that is planned is the type of pelimpah (head work) with a height of Check Dam 8.5 m. Tilt of the body at the upstream 0.6, the distance between the main dam and sub dam 25.2 m, the thickness of the apron floor 1.6 m, with an estimated volume of sediment flow that can be accommodated by 14797.6 m3. The stability of the Check Dam construction was obtained at a value of 3.43 >1.5 and a sliding of 1.53 > 1.5 with a safety coefficient of 1.5, so that the construction of the Check Dam was stable.

scholarly journals Stability-indicating HPLC-DAD method for the determination of empagliflozin

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Shilpi Pathak ◽  
Pradeep Mishra
Keyword(s):  
Hplc Method ◽  
Coefficient Of Determination ◽  
Forced Degradation ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Forced Degradation Studies ◽  
Tablet Dosage

Abstract Background A stability-indicating RP-HPLC method was developed and validated for the estimation of empagliflozin drug and its tablet dosage form using a DAD detector. The mobile phase consisted of methanol/acetonitrile/0.1%OPA (75:20:5). The peak was observed at 2.54 min using 222.0 nm absorption maxima. Results Calibration curve plot was found within the range of 10–50 µg/mL. The coefficient of determination (R2) was found to be 0.9990. Forced degradation studies were performed for the empagliflozin in various conditions, and the results were calculated as %RSD values and were found to be within the limits. Conclusion The method was validated as per ICH guidelines with respect to all validation parameters.

scholarly journals Quantitative determination of ciprofloxacin and levofloxacin antibacterials by Spectrophotometeric and high performance liquid chromatography

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad Akram ◽  
Jamil Anwar ◽  
Ammar Z. Alshemarya ◽  
Yi-Fan Goh ◽  
Ahmed Sher Awan ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Drug Effect ◽  
Hplc Method ◽  
Linear Calibration ◽  
Pharmaceutical Tablets ◽  
Pure Compounds ◽  
The Stability

A simple, rapid and sensitive Spectrophotometeric method for the determination of fluoroquinolones; ciprofloxacin and levofloxacin have been performed in pure form and pharmaceutical tablets. Both drugs gave reddish complexes when treated with iron (III) chloride at pH 4.0. The drugs showed maximum absorption at 530 and 545 nm. In both cases linear calibration was obtained up to 0.9 mg/10 mL of the drug. Effect of different parameters like pH, temperature and time was also studied on the stability of the complexes. The percentage recoveries found by described method was in the range of 98.2---100.01 %. Standards were prepared from the pure compounds obtained from sigma-Aldrich Pharm. The method was successfully employed for the Assay of drugs in commercial formulations. Finally determination of the drugs was carried out through HPLC method which showed that there is no appreciable difference between the results of both the methods. Results revealed that proposed method is practically suitable for routine applications in quality control laboratories for the analysis of fluoroquinolones drugs.________________________________________GRAPHICAL ABSTRACT

scholarly journals A Stability Indicating RP-HPLC Method for the Estimation of Gemcitabine HCl in Injectable Dosage forms

2010 ◽  
Vol 7 (s1) ◽  
pp. S239-S244 ◽  
Author(s):  
Shaik Mastanamma ◽  
G. Ramkumar ◽  
D. Anantha Kumar ◽  
J. V. L. N. Seshagiri Rao
Keyword(s):  
Dosage Forms ◽  
Hplc Method ◽  
Forced Degradation ◽  
Stability Indicating ◽  
Gemcitabine Hydrochloride ◽  
Rp Hplc ◽  
Forced Degradation Studies ◽  
The Stability ◽  
Injectable Dosage

A stability indicating RP HPLC method has been developed for the determination of gemcitabine hydrochloride. Chromatography was carried out on an ODS C18column (250×4.6 mm; 5μ) using a mixture of methanol and phosphate buffer (40: 60 v/v ) as the mobile phase at a flow rate of 1.0 mL/min. The detection of the drug was monitored at 270 nm. The retention time of the drug was found to be 2.31 min. The method produced linear responses in the concentration range of 10 to 60 μg/mL of gemcitabine HCl. The method was found to be reproducible for analysis of the drug in injectable dosage forms. The stability of the drug was assessed by forced degradation studies.

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