scholarly journals Can the Diagnosis of Recurrent Vulvovaginal Candidosis Be Improved by Use of Vaginal Lavage Samples and Cultures on Chromogenic Agar?

2002 ◽  
Vol 10 (2) ◽  
pp. 89-92 ◽  
Author(s):  
N. Novikova ◽  
A. Rodrigues ◽  
P.-A. Mårdh
Keyword(s):  
Candida Albicans ◽  
Recovery Rate ◽  
Detection Rate ◽  
Rapid Identification ◽  
Biochemical Tests ◽  
Vaginal Lavage ◽  
Yeast Infection ◽  
Sabouraud Agar ◽  
Chromogenic Agar ◽  
Vulvovaginal Candidosis

Objective:To investigate if introital and vaginal flushing samples inoculated on chromogenic agar could increase the recovery rate and rapid identification ofCandidaand non-albicansspecies, as compared to culture of posterior vaginal fornix samples on Sabouraud agar and speciation of isolates by biochemical tests.Methods:Samples from the introitus and the posterior vaginal fornix and vaginal lavage samples were collected from 91 women with a history suggestive of recurrent vulvovaginal candidosis (RVVC), and with a suspected new attack of the condition. The specimens were cultured on Sabouraud and CHROMagar®. Speciation of yeast isolates was made on the chromogenic agar by API 32C®kits and by an atomized system (Vitek®).Results:Forty-six (51%) women were positive forCandidafromone or more of the samples. The introital cultures were positive in 43 (47%) women, both on Sabouraud and chromogenic agar. From the posterior vaginal fomix, 42 (46%) women were positive on the Sabouraud and 43 (47%) on chromogenic agar cultures, while the vaginal lavage cultures yieldedCandidaon those two media in 40 (44%) and 4l (45%) cases, respectively.Candida albicanswas the most frequent species recovered, from 40 (87%) cases, followed byC. kruseiin 4 (9%),C. glabratain 2 (4%), andC. parapsilosisin one case. There was only onewoman who had a mixed yeast infection, byC. albicansandC. krusei. There was only one discrepancy in the speciation as demonstrated by mean of chromogenic agar and API 32C kit.Conclusions:Neither cultures of introital nor of vaginal lavage samples increases the detection rate ofCandidain RVVC cases as compared to cultures of posterior vaginal fornix samples. Use of chromogenic agar is a convenient and reliable means to detect colonization byCandidaand differentiate betweenC. albicansand non-albicansspecies.

scholarly journals Prevalence of Candida albicans among Female Patients in Two Selected Hospitals in Owerri Metropolis, Imo State Southeastern Nigeria

2020 ◽  
pp. 1-8
Author(s):  
M. O. Nwachukwu ◽  
J. N. Azorji ◽  
P. C. Onyebuagu ◽  
L. A. Adjeroh ◽  
S. N. Nmezi
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Candida Albicans ◽  
Structured Interview ◽  
Female Reproductive Tract ◽  
Biochemical Tests ◽  
Southeastern Nigeria ◽  
Sabouraud Agar ◽  
Female Patients ◽  
Tract Infections

The incidence of candidiasis in the female reproductive tract is a serious threat to public health. This study assesses the prevalence of Candida albicans among female patients in the two selected hospitals in Owerri metropolis. A systematic random sampling technique was used to select 120 female patients between the ages of 16-56 years. Only patients who showed no symptoms of urinary tract infections and who were not on anti-fungal therapy at the time of the study were included in the study. A well-structured interview questionnaire was used to source information on socio-demographic characteristics of the respondents as well as the risk factors of Candida albicans infections. High Vaginal Swab (HVS) was aseptically collected from each of the patients, using a sterile swab stick. The collected samples were labeled appropriately and immediately sent to the microbiology/mycology lab for analysis. The HVS samples were respectively streaked directly into sabouraud agar plates and incubated anaerobically at 37oC for 48 hours. Yeast growth characteristics were noted. Positive colonies were sub-cultured in Macconkey agar to obtain pure isolates. The Candida albicans were properly identified and confirmed by germ tube test, gram staining and biochemical tests. The results showed that the overall prevalence of Candida albicans among the women was (53.34%). The prevalence was highest among the patients in the age group 16-25 (44.17%), lower education qualification (n=65, 54.17%) and pregnant women (n=44, 36.67%). The possible risk factors for the infection include use of antibiotics (n=85, 70.83%), use of nylon underwear (n=78, 65.00), use of public toilet (n=90, 75.00%), use of squat WC (n=59, 9.17%) and use of oral contraceptive (n=59, 49.17%). The results call for preventive measures to protect women. Therefore public health education and campaign should be adopted.

scholarly journals Candida spp. occurrence in oral cavities of breastfeeding infants and in their mothers' mouths and breasts

2003 ◽  
Vol 17 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Maria Stella Amorim da Costa Zöllner ◽  
Antonio Olavo Cardoso Jorge
Keyword(s):  
Oral Cavity ◽  
Candida Species ◽  
Statistical Difference ◽  
Control Group ◽  
Biochemical Tests ◽  
Candida Spp ◽  
Lactating Women ◽  
Sabouraud Agar ◽  
Breastfed Infants ◽  
Yeast Strains

This study aimed to determine the occurrence of Candida spp. in the oral cavity of predominantly breastfed infants and in their mothers' mouths and breasts, as well as in the oral cavity of bottlefed infants and in non-lactating women. One hundred and sixty nine women and eighty-five milk-fed infants took part in this study and were divided into four groups: 1) infants predominantly on breastfeeding (n = 55) and their mothers (n = 55); 2) infants on bottlefeeding (n = 30); 3) non-lactating women on whom oral collections were performed (n = 80) and, 4) non-lactating women on whom breast collections were performed (n = 34). Oral and mammary swabs were cultured on Sabouraud agar dextrose with chloramphenicol. The Candida yeast strains found were isolated and identified through morphological and biochemical tests. Candida species were much less frequent in infants who were predominantly breastfed than in those who were bottlefed. Yeasts were much more frequent on the breasts of lactating women, with statistical difference in relation to the control group.

scholarly journals Gene Sequence Analysis of Mitochondrial COI in Genus Saperda (Coleoptera: Cerambycidae: Lamiinae)

2021 ◽  
Author(s):  
Wei XU ◽  
Qinhua Gan ◽  
Jian Pu ◽  
Yingwen Pan ◽  
Bo Cai ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Detection Rate ◽  
Gene Sequence ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Nuclear Gene ◽  
Rapid Identification ◽  
Mitochondrial Coi ◽  
Gene Sequence Analysis ◽  
Total Dna

In this study, the total DNA of nine species of Saperda (Lopezcolonia) octopunctata (Scopoli, 1772), Saperda (Lopezcolonia) scalaris (Linnaeus, 1758), Saperda interrupta Gebler, Saperda Alberti (Plavilstshikov), Saperda (Saperda) similis Laicharting, 1784, Saperda (Compsidia) populnea (Linnaeus, 1758), Saperda (Saperda) carcharias (Linnaeus, 1758), Saperda (Lopezcolonia) perforata Pallas, 1773 and Saperda ohbayashi were extracted. Two partial sequences of mitochondrial gene and one partial sequence of nuclear gene were amplified. Comparing the COI sequence with the DNA barcode data in GenBank can effectively identify the related species of Saperda. It will be applied to the rapid identification of some species of Saperda in imported wood at ports, and improve the detection rate of plant quarantine.

scholarly journals Comparative evaluation of macrodilution and chromogenic agar screening for determining fluconazole susceptibility of Candida albicans.

1996 ◽  
Vol 34 (12) ◽  
pp. 3237-3239 ◽  
Author(s):  
T F Patterson ◽  
W R Kirkpatrick ◽  
S G Revankar ◽  
R K McAtee ◽  
A W Fothergill ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Comparative Evaluation ◽  
Chromogenic Agar

scholarly journals Evaluación de la diversidad de bacterias lácticas y levaduras en quesos frescos de cabra de la quebrada de Humahuaca

2015 ◽  
Vol 13 (1) ◽  
pp. 03
Author(s):  
E Gustavo Ancasi ◽  
S Maldonado ◽  
R Oliszewski
Keyword(s):  
Candida Albicans ◽  
Kluyveromyces Lactis ◽  
Debaryomyces Hansenii ◽  
Pichia Anomala ◽  
Dekkera Bruxellensis ◽  
Sabouraud Agar ◽  
Zygosaccharomyces Rouxii ◽  
Candida Magnoliae ◽  
Candida Versatilis

Los quesos frescos de cabra artesanales de la quebrada de Humahuaca son elaborados con leche cruda, cuya maduración genera sabores, aromas y texturas característicos de la región. Los objetivos de este estudio fueron identificar y caracterizar bacterias lácticas (BAL) y levaduras nativas, aisladas de quesos frescos de esta zona productora. De un total de 36 muestras sembradas en agar Sabouraud, agar MRS y M17, se obtuvieron 128 levaduras y 39 lactobacilos, los que fueron identificados fenotípicamente y evaluadas las siguientes propiedades tecnológicas: pH a la coagulación, tasa de acidificación, proteólisis en agar leche, lipólisis en agar triacetina, producción de acetoína en leche reconstituida y asimilación del citrato en agar citrato. Lb. delbruekii subsp. bulgaricus, Lb. casei subsp. pseudoplantarum, Lb. plantarum var. arabinosus, Lb. plantarum var. plantarum, Lb. casei subsp. rhamnosus, Lb. acidophilus, Lb. helveticus, Lb. fermentum, Lb. brevis var. brevis, Lactococos sp. y Enterococcus sp. fueron las bacterias lácticas identificadas. Del total de los aislamientos, 41,6% coagularon la leche en 10 horas y 33% en 5 horas. Lb. helveticus coaguló la leche a pH de 5,40 en 5 horas, hasta alcanzar un valor final de 4,16 en 24 h, mientras que Lb. delbrueckii subsp. bulgaricus y Lb. fermentum iniciaron la coagulación en 5 horas, con valores de pH iniciales de 4,81 y 4,92 hasta valores finales de 4,19 y 4,21 respectivamente. Lb. helveticus, Lb. delbrueckii subsp. bulgaricus, Lb. plantarum var. arabinosus, Lb. fermentum, Lb. casei subsp. rhamnsosus, Lb. casei subsp. pseudoplantarum, Lb. brevis var. brevis, en orden descendente, demostraron tener capacidad acidificante. Lb. fermentum y Lb. casei subsp. pseudoplantarum desarrollaron actividad proteolítica y sólo Lb. plantarum var. plantarum demostró tener actividad lipolítica. Las levaduras aisladas fueron Debaryomyces hansenii, Zygosaccharomyces rouxii, Kluyveromyces lactis, Wickerbamiela domerquiae, Dekkera bruxellensis, Candida valdiviana, Candida novakii, Dekkera bruxellensis, Candida versatilis, Candida magnoliae, Candida albicans, Pichia anómala, Dekkera anómala y Rodotorula sp. Cepas de D. hansenii, C. magnoliae, Z. rouxii,C. versatilis y K. lactis tuvieron actividad proteolítica y lipólitica, y una cepa de W. domerquiae tuvo solamente actividad proteolítica. Algunas cepas de K. lactis produjeron acetoína y D. bruxellensis y C. versatilis metabolizaron el citrato, hidrolizaron la caseína y tuvieron actividad lipolítica.  Los resultados obtenidos en este estudio muestran que la composición de las poblaciones de BAL y levadura en quesos artesanales es específica de la región. Los conocimientos adquiridos en este estudio podrían ser utilizados para la obtención de cultivos iniciadores con cepas de BAL y levaduras específicas de la región, destinados a la producción de quesos frescos con origen geográfico específico.

CHROMagarTM Candida Plus: A novel chromogenic agar that permits the rapid identification of Candida auris

2020 ◽  
Author(s):  
Andrew M Borman ◽  
Mark Fraser ◽  
Elizabeth M Johnson
Keyword(s):  
Candida Species ◽  
Rapid Identification ◽  
Hospital Environment ◽  
Candida Auris ◽  
Large Hospital ◽  
Pathogenic Yeast ◽  
Isolation Medium ◽  
Wide Range ◽  
Chromogenic Agar ◽  
Environment Identification

Abstract   Candida auris is a serious nosocomial health risk, with widespread outbreaks in hospitals worldwide. Successful management of such outbreaks has depended upon intensive screening of patients to identify those that are colonized and the subsequent isolation or cohorting of affected patients to prevent onward transmission. Here we describe the evaluation of a novel chromogenic agar, CHROMagarTM Candida Plus, for the specific identification of Candida auris isolates from patient samples. Candida auris colonies on CHROMagarTM Candida Plus are pale cream with a distinctive blue halo that diffuses into the surrounding agar. Of over 50 different species of Candida and related genera that were cultured in parallel, only the vanishingly rare species Candida diddensiae gave a similar appearance. Moreover, both the rate of growth and number of colonies of C. auris recovered from swabs of pure and mixed Candida species were substantially increased on CHROMagarTM Candida Plus agar when compared with growth on the traditional mycological isolation medium, Sabouraud dextrose agar. Taken together, the present data suggest that CHROMagarTM Candida Plus agar is an excellent alternative to current conventional mycological media for the screening of patients who are potentially colonized/infected with Candida auris, can be reliably used to identify this emerging fungal pathogen, and should be tested in a clinical setting. Lay Abstract Candida auris is a novel pathogenic yeast that has been associated with large hospital outbreaks across several continents. Affected patients become colonized, predominantly on the skin, with large quantities of C. auris which they then shed into the hospital environment. Identification of C. auris is challenging using routine laboratory methods, and time consuming when patients are colonized with a mixture of different Candida species. Here we demonstrate that a novel chromogenic agar, CHROMagarTM Candida Plus, permits the rapid differentiation of C. auris from a wide range of other yeast species and is potentially ideally suited to screening of patients that are suspected of being colonized or infected with this medically important yeast.

Comparative study of biochemical methods for the identification of Dichelobacter nodosus

2004 ◽  
Vol 52 (3) ◽  
pp. 267-273
Author(s):  
Ruth Jiménez ◽  
S. Píriz ◽  
E. M. Mateos ◽  
S. Vadillo
Keyword(s):  
Liquid Chromatography ◽  
Comparative Study ◽  
Rapid Identification ◽  
Gas Liquid Chromatography ◽  
Correct Identification ◽  
Type Collection ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Biochemical Methods ◽  
Dichelobacter Nodosus

The aim of this study was to compare four identification procedures to detect Dichelobacter nodosus and develop a rapid, simple and effective method to identify D. nodosus strains isolated from cases of ovine footrot. The four methods used were: (a) the classic guidelines set down by Holdeman et al. (1977) and Summanen et al. (1993) which are based on gas liquid chromatography (GLC) and different biochemical tests, this method was considered as landmark; (b) Baron and Citron's flowchart for the rapid identification of Gram-negative rod-shaped anaerobes (1997); (c) the API rapid 32 A system (bio Mérieux), and (d) Mast ID™ Anaerobe ID Ring (MID8) (Mast Diagnostics). None of the four methods used allowed us to correctly identify the D. nodosus strains (neither the strains isolated from cases of ovine footrot nor those originating from type collection). Because of the difficulties encountered in obtaining a correct identification of D. nodosus, we propose a simple, rapid and effective way to achieve this task. Our flowchart will provide the means to identify this microorganism in any laboratory of general microbiology without having to use any specialised equipment.

scholarly journals A novel multiplex-PCR for the rapid identification of Mycobacterium bovis in clinical isolates of both veterinary and human origin

2003 ◽  
Vol 130 (3) ◽  
pp. 485-490 ◽  
Author(s):  
L. COBOS-MARÍN ◽  
J. MONTES-VARGAS ◽  
S. RIVERA-GUTIERREZ ◽  
A. LICEA-NAVARRO ◽  
J. A. GONZÁLEZ-Y-MERCHAND ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Clinical Isolate ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Rapid Identification ◽  
Human Infection ◽  
Economic Losses ◽  
Human Origin ◽  
Biochemical Tests ◽  
Reliable Identification

Bovine tuberculosis is a zoonotic disease that not only causes huge economic losses but also poses an important risk for human infection. The definitive identification of a clinical isolate relies on time-consuming, highly specialized and laborious biochemical tests. We have developed a method for the rapid and reliable identification of Mycobacterium bovis and for its simultaneous differentiation from other members of the M. tuberculosis complex. Furthermore, the technique also allowed us to distinguish M. tuberculosis complex members from other Mycobacterial species. The method comprises both a single PCR and a multiplex-PCR and can be confidently applied to samples of both veterinary and human origin.

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