Abstract 15702: Chronic Use of Electronic or Tobacco Cigarettes Alters Circulating Polyunsaturated Fatty Acids, Oxidation Status and Cardiovascular Risk in Healthy Young Adults

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Rajat Gupta ◽  
Yan Lin ◽  
Karla Luna ◽  
Anjali Logue ◽  
Alexander Yoon ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Young Adults ◽  
Cardiovascular Risk ◽  
Polyunsaturated Fatty Acids ◽  
Electronic Cigarettes ◽  
Plasma Concentrations ◽  
Multivariable Analysis ◽  
The United States ◽  
Heme Oxygenase 1 ◽  
Chronic Use

Introduction: Electronic cigarettes (EC) have recently gained unprecedented popularity with over 5 million users in the United States. However, it is not known whether chronic EC use induces cardiovascular health effects in healthy young adults. Hypothesis: We hypothesized that chronic EC use ≥1 year affects lipid peroxidation and/or antioxidant defense in the circulating blood, as compared to chronic tobacco cigarette (TC) smokers. Methods: We enrolled EC-users (n=32), TC-smokers (n=29) and non-users (n=45) with mean ages of 28.3, 27.8 and 27.4 years, respectively. Plasma concentrations of polyunsaturated fatty acids and oxidized metabolites were assessed by liquid chromatography coupled to ion trap mass spectrometry. Total antioxidant capacity (TAC), heme oxygenase-1 (HO-1) and functional activity of paraoxonase1 (PON1) and arylesterase were determined by colorimetric and enzymatic assays in the plasma. Multivariable analysis was performed using classification models aimed at segregating subjects based on biomarker profiles. Results: As shown in table 1, EC-users and TC-smokers exhibited significant alterations in oxidized linoleic acid (LA) metabolites (9- and 13-hydroxyoctadecadienoic acid (HODE)), arachidonic acid (AA), LA, TAC and HO-1. Multivariable analysis identified a set of 5 biomarkers (13-HODE, LA, 9-HODE, 12-hydroxyeicosatetraenoic acid (HETE), and AA) that discriminated EC-users from TC-smokers and non-users with an overall accuracy of 73.4%. Conclusions: Chronic use of EC induces both common (i.e. reduced 9- and 13-HODEs, and increased TAC) as well as differential effects (i.e. altered concentrations of AA and LA) to those induced by TC, along with intermediate plasma HO-1 concentrations, suggesting that EC, likewise TC smoke, could impact cardiovascular risk.

scholarly journals Electronic and Tobacco Cigarettes Alter Polyunsaturated Fatty Acids and Oxidative Biomarkers

2021 ◽  
Author(s):  
Rajat Gupta ◽  
Yan Lin ◽  
Karla Luna ◽  
Anjali Logue ◽  
Alexander J Yoon ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Cardiovascular Risk ◽  
Polyunsaturated Fatty Acids ◽  
Low Density Lipoprotein ◽  
Plasma Concentrations ◽  
Multivariable Analysis ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Heme Oxygenase 1

Rationale: Chronic electronic cigarette (EC) users exhibit a higher susceptibility of low-density lipoprotein (LDL) to undergo oxidation as compared to non-user controls. However, there is a paucity of data regarding EC effects on lipid peroxidation in the blood and their relationship to cardiovascular risk. Objective: To test the hypothesis that chronic (≥1 year) EC use exerts intermediate effects on plasma lipid peroxidation and/or antioxidant defense compared to chronic tobacco cigarette (TC) smoking. Methods and Results: We enrolled EC-users (n=32), TC-smokers (n=29) and non-users (n=45), with mean ages of 28.3, 27.8 and 27.4 years, respectively. Plasma concentrations of free polyunsaturated fatty acids and oxidized metabolites were assessed by mass spectrometry. Total antioxidant capacity (TAC), concentrations of glutathione, bilirubin, heme oxygenase-1 (HO-1), and functional activity of paraoxonase1 (PON1) were determined by colorimetric and enzymatic assays. Multivariable analysis was performed using classification models for segregating participants based on biomarker profiles. Plasma arachidonic acid (AA) concentration was higher in TC-smokers but lower in EC-users, together with linoleic acid (LA) concentration, as compared to TC-smokers and non-users (p<0.05). Oxidized LA metabolites (9- and 13-hydroxyoctadecadienoic acid (HODE)) were lower in EC-users and TC-smokers as compared to non-users (p<0.001). Consistently, TAC and bilirubin were elevated in EC-users and TC-smokers as compared to non-users (p<0.05). Of interest, plasma HO-1 concentration was higher in TC-smokers as compared to non-users (p=0.01) with intermediate levels in EC-users. Multivariable analysis identified 5 biomarkers (13-HODE, LA, 9-HODE, 12-hydroxyeicosatetraenoic acid (HETE), AA) that discriminated EC-users from TC-smokers and non-users with an accuracy of 73.4%. Conclusions: Chronic use of EC induces common (i.e. lower 9- and/or 13-HODEs and higher TAC and bilirubin) as well as differential effects (i.e. altered AA and LA concentrations) to those induced by TC, along with intermediate plasma HO-1 concentration, suggesting that EC, likewise TC smoke, could impact cardiovascular risk.

Plasma glycerophospholipid profile, erythrocyte n–3 PUFAs, and metabolic syndrome incidence: a prospective study in Chinese men and women

2021 ◽  
Author(s):  
Shuangshuang Chen ◽  
Qingqing Wu ◽  
Li Zhu ◽  
Geng Zong ◽  
Huaixing Li ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Animal Studies ◽  
Plasma Concentrations ◽  
Positive Association ◽  
Community Dwelling ◽  
P Value ◽  
Total N ◽  
Long Chain

ABSTRACT Background Animal studies have highlighted critical roles of glycerophospholipid (GP) metabolism in various metabolic syndrome (MetS)-related features such as dyslipidemia, obesity, and insulin resistance. However, human prospective studies of associations between circulating GPs and risks of MetS are scarce. Objectives We aimed to investigate whether GPs are associated with incidence of MetS in a well-established cohort. Methods A total of 1243 community-dwelling Chinese aged 50–70 y without MetS at baseline and followed up for 6 y were included in current analyses. A total of 145 plasma GPs were quantified by high-throughput targeted lipidomics. MetS was defined using the updated National Cholesterol Education Program Adult Treatment Panel III criteria for Asian Americans. Results After 6 y, 429 participants developed MetS. Eleven GPs, especially those with long-chain polyunsaturated fatty acids (LCPUFAs) or very-long-chain polyunsaturated fatty acids (VLCPUFAs) at the sn-2 position, including 1 phosphatidylcholine (PC) [PC(18:0/22:6)], 9 phosphatidylethanolamines (PEs) [PE(16:0/22:6), PE(18:0/14:0), PE(18:0/18:1), PE(18:0/18:2), PE(18:0/20:3), PE(18:0/22:5), PE(18:0/22:6), PE(18:1/22:6), and PE(18:2/22:6)], and 1 phosphatidylserine (PS) [PS(18:0/18:0)], were positively associated with incident MetS (RRs: 1.16–1.30 per SD change; Bonferroni-corrected P &lt; 0.05). In network analysis, the strongest positive association for MetS incidence was evidenced in a module mainly composed of PEs containing C22:6 and PSs [RR: 1.21; 95% CI: 1.12, 1.31 per SD change; Bonferroni-corrected P &lt; 0.05]. This association was more pronounced in participants with lower erythrocyte total n–3 PUFA concentrations [Bonferroni-corrected Pinter(P value for the interaction)&lt; 0.05]. Conclusions Elevated plasma concentrations of GPs, especially PEs with LCPUFAs or VLCPUFAs at the sn-2 position, are associated with higher risk of incident MetS. Future studies are merited to confirm our findings.

scholarly journals Plasma fatty acids in de novo lipogenesis pathway are associated with diabetogenic indicators among adults: NHANES 2003–2004

2018 ◽  
Vol 108 (3) ◽  
pp. 622-632 ◽  
Author(s):  
Elaine A Yu ◽  
Peter J Hu ◽  
Saurabh Mehta
Keyword(s):  
Fatty Acids ◽  
De Novo ◽  
Plasma Concentrations ◽  
The United States ◽  
Structure Study ◽  
De Novo Lipogenesis ◽  
Group 3 ◽  
Group 2 ◽  
Study Participants ◽  
Group 1

ABSTRACT Background Insulin regulates fatty acids (FAs) in the blood; conversely, FAs may mediate insulin sensitivity and are potentially modifiable risk factors of the diabetogenic state. Objective The objective of our study was to examine the associations between plasma concentrations of FAs, fasting plasma glucose (FPG), and glycated hemoglobin (HbA1c) among individuals (n = 1433) in the NHANES (2003–2004). Design Plasma concentrations of 24 individual FAs were considered individually and in subgroups, per chemical structure. Study participants were categorized in diabetogenic groups: Group 1 (HbA1c ≥6.5% or FPG ≥126 mg/dL), Group 2 (HbA1c 5.7% to &lt;6.5% or FPG 100 to &lt;126 mg/dL), and Group 3 (HbA1c &lt;5.7% and FPG &lt;100 mg/dL). We assessed associations between diabetogenic groups and plasma FAs in multivariate multinomial regressions (with Group 3 as the reference). Results Overall, 7.0% of study participants were in Group 1; 33.3% were in Group 2. Plasma concentrations of several individual FAs, including even-chain saturated FAs (SFAs; myristic, palmitic, stearic acids) and monounsaturated FAs (MUFAs; cis-vaccenic, oleic acids), were respectively associated with greater odds of Groups 1 and 2 status, adjusting for covariates. Higher concentrations of SFA and MUFA subgroups (highest compared with lowest quartile) were associated with increased odds of Group 2 status [SFAs adjusted OR (aOR): 1.51 (95% CI: 1.05, 2.18); MUFAs aOR: 1.78 (95% CI: 1.11, 2.85)]. Higher eicosapentaenoic acid plasma concentration was associated with decreased odds of Group 1 status [quartile 4 aOR: 0.41 (95% CI: 0.17, 0.95)]. Conclusions Higher plasma concentrations of SFAs and MUFAs, primary de novo lipogenesis products, were associated with elevated FPG and HbA1c in a nationally representative study population in the United States. Additional studies are necessary to elucidate potential causal relationships between FAs (from endogenous production and dietary consumption) and diabetogenic indicators, as well as clinical implications for managing diabetes and prediabetes.

scholarly journals Polyunsaturated fatty acids in the food chain in the United States

2000 ◽  
Vol 71 (1) ◽  
pp. 179S-188S ◽  
Author(s):  
PM Kris-Etherton ◽  
Denise Shaffer Taylor ◽  
Shaomei Yu-Poth ◽  
Peter Huth ◽  
Kristin Moriarty ◽  
...  
Keyword(s):  
United States ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Food Chain ◽  
The United States
Sign in / Sign up

Export Citation Format

Share Document